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State of the art solid-state MAS NMR spectroscopy.

NMR spectroscopy for the structure/dynamics studies of biological and functional materials.

Akbey Lab is using advanced solid-state NMR spectroscopy to perform structural biology of difficult proteins. Our main focus is functional amyloids forming the basis of bacterial biofilms and microbial macrostructures. These two classes of proteins are facilitating many bacterial infections affecting a broad range of people, and as a result have a significant medical relevance.

We complement our main experimental technique, i.e. solid-state NMR, by solution-state NMR, X-Ray crystallography, electron microscopy, a variety of biophysical methods and recombinant protein production.

Our research is interdisciplinary lying in between many disciplines including chemistry, biology and nanoscience. With a focused set of people working on topics such as NMR method development and spectroscopy, protein biophysics and biological/functional material characterization, we aim to solve difficult problems.

E. Coli in its native biofilm. The amyloid fibrils and EPS is seen around the bacteria.
E. Coli as a -EPS mutant.
Zoom into the amyloid fibril of E. Coli biofilm -EPS. Long fibers are CsgA/CsgB protein.
2D 13C-13C MAS NMR spectra of SH3 domain recorded at room-temperature. This NMR spectrum contains information about the amino acid chemical shifts that are utilized to obtain structure of proteins.
A different representation of the 2D 13C-13C MAS NMR spectrum.