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						<h1 itemprop="headline">Specialized iNANO Lecture: Pushing the Limits in Fluorescence Microscopy: Highly Multiplexed Super-Resolution Imaging With DNA-PAINT</h1>
						
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							<p class="text--intro" itemprop="description">Postgraduate researcher Johannes Woehrstein, Wyss Institute for Biologically Inspired Engineering, Center for Life Science, Boston, Massachusetts, United States</p>
						
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														Friday 14  March 2014,
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														&nbsp;at 14:15 -  15:00
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														<p>Teaching room 1593-226 of the iNANO House, Gustav Wieds vej 14, 8000 Aarhus C</p>
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														<span itemprop="name">Trine Møller Hansen</span>
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									<table cellpadding="2" cellspacing="2" class="contenttable"> <tbody> <tr> <td>&nbsp;</td> <td> <h2><a href="http://yin.hms.harvard.edu/" target="_self" data-htmlarea-external="1">Postgraduate researcher Johannes Woehrstein, Wyss Institute for Biologically Inspired Engineering, Center for Life Science, Boston, Massachusetts, United States</a></h2> <h3><em><strong>Pushing the Limits in Fluorescence Microscopy: Highly Multiplexed Super-Resolution Imaging With DNA-PAINT</strong></em></h3> <p>Super-resolution fluorescence microscopy is a powerful tool for biological research, but obtaining multiplexed images for a large number of distinct target species remains challenging. In DNA-PAINT the transient binding of short fluorescently labeled oligonucleotides is used for simple and easy-to-implement multiplexed super-resolution imaging that achieves sub-10-nm spatial resolution. By labeling target proteins with antibody-DNA conjugates, DNA-PAINT is also applicable to in situ cell imaging in two and three dimensions.</p> <p>We recently used the unique programmability of DNA binding interactions in DNA-PAINT imaging to develop a novel multiplexing approach we call Exchange-PAINT. This allows sequential imaging of multiple targets using only a single dye and a single laser source. Using Exchange-PAINT, we experimentally demonstrated ten-color sub-10 nm super-resolution imaging in vitro as well as four-color two-dimensional imaging and three-color 3D imaging of proteins in fixed cells.</p> </td> </tr> <tr> <td>&nbsp;</td> <td><strong>Hosts:</strong>&nbsp;Professor Kurt Gothelf &amp; PhD student Anne Louise Kodal, iNANO, CDNA &amp; Dept. of Chemistry, Aarhus University, Aarhus&nbsp;</td> </tr> </tbody> </table>
								
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