Andersen, M. Ø., Nygaard, J. V., Howard, K., Bak, R. O., Paludan, S. R., Raarup, M. K., Besenbacher, F. & Kjems, J. (2007).
Surface delivery of siRNA for implants.
European Cells & Materials,
14(3), 118.
Andersen, M. Ø., Nygaard, J. V., Burns, J. S.
, Raarup, M. K., Nyengaard, J. R., Bünger, C., Besenbacher, F., Howard, K. A., Kassem, M. & Kjems, J. (2010).
siRNA nanoparticle functionalization of nanostructured scaffolds enables controlled multilineage differentiation of stem cells.
Molecular Therapy,
18(11), 2018-2027.
https://doi.org/10.1038/mt.2010.166
Andersen, M. Ø., Howard, K. A., Paludan, S. R., Besenbacher, F. & Kjems, J. (2008).
Delivery of siRNA from lyophilized polymeric surfaces.
Biomaterials,
29(4), 506-512.
https://doi.org/10.1016/j.biomaterials.2007.10.003
Berton, M.
, Howard, K., Benimetskaya, L., Allemann, E., Weil, R., Zwillenberg, L. O., Stein, C. A. & Gurny, R. (1997).
Uptake of oligonucleotide loaded nanoparticles in prostatic cancer cells and their intracellular localization.
Proceedings of the Controlled Release Society, (24), 669-670.
Bienk, K., Hvam, M. L., Pakula, M. M., Dagnæs-Hansen, F., Wengel, J., Malle, B. M.
, Kragh-Hansen, U., Cameron, J., Bukrinski, J. T.
& Howard, K. (2016).
An albumin-mediated cholesterol design-based strategy for tuning siRNA pharmacokinetics and gene silencing.
Journal of Controlled Release,
232, 143-151.
https://doi.org/10.1016/j.jconrel.2016.04.013
Blans, K. I. M., Hansen, M. S., Sørensen, L. V.
, Hvam, M. L., Howard, K., Möller, A., Wiking, L., Larsen, L. B. & Rasmussen, J. T. (2017).
Pellet-free isolation of human and bovine milk extracellular vesicles by size-exclusion chromatography.
Journal of Extracellular Vesicles,
6(1), Article 1294340.
https://doi.org/10.1080/20013078.2017.1294340
Cai, Y., López-Ruiz , E., Wengel, J., Creemers, L.
& Howard, K. (2017).
A hyaluronic acid-based hydrogel enabling CD44-mediated chondrocyte binding and gapmer oligonucleotide release for modulation of gene expression in osteoarthritis.
Journal of Controlled Release,
253(May), 153-159.
https://doi.org/10.1016/j.jconrel.2017.03.004
Caspersen , M. B.
, Kuhlmann, M., Nicholls , K., Saxton , M. J., Andersen , B., Bunting , K., Cameron, J.
& Howard, K. (2017).
Albumin-based drug delivery using cysteine 34 chemical conjugates; important considerations and requirements.
Therapeutic Delivery,
8(7), 511-519.
https://doi.org/10.4155/tde-2017-0038
Chen, M., Gao, S., Dong, M., Song, J., Yang, C., Howard, K. A., Kjems, J. & Besenbacher, F. (2012).
Chitosan/siRNA nanoparticles encapsulated in PLGA nanofibers for siRNA delivery.
A C S Nano,
6(6), 4835-4844.
https://doi.org/10.1021/nn300106t
Christiansen, S. H., Zhang, X., Juul-Madsen, K., Hvam, M. L., Vad, B. S., Behrens, M. A., Thygesen, I. L., Jalilian, B., Pedersen, J. S., Howard, K., Otzen, D. E. & Vorup-Jensen, T. (2017).
The random co-polymer glatiramer acetate rapidly kills primary human leukocytes through sialic-acid-dependent cell membrane damage.
B B A - Biomembranes,
1859(3), 425-437.
https://doi.org/10.1016/j.bbamem.2017.01.001
Christiansen, S. H., Murphy, R. A.
, Juul-Madsen, K., Fredborg, M., Hvam, M. L., Axelgaard, E., Skovdal, S. M., Meyer, R. L., Sorensen, U. B. S., Moeller, A., Nyengaard, J. R., Norskov-Lauritsen, N., Wang, M., Gadjeva, M.
, Howard, K. A., Davies, J. C.
, Petersen, E. & Vorup-Jensen, T. (2017).
The Immunomodulatory Drug Glatiramer Acetate is Also an Effective Antimicrobial Agent that Kills Gram-negative Bacteria.
Scientific Reports,
7(1), Article 15653.
https://doi.org/10.1038/s41598-017-15969-3
Decuzzi, P., Peer, D., Di Mascolo, D., Palange, A. L., Manghnani, P. N., Moein Moghimi, S., Shadi Farhangrazi, Z.
, Howard, K. A., Rosenblum, D., Liang, T., Chen, Z., Wang, Z., Zhu, J. J., Gu, Z., Korin, N., Letourneur, D., Chauvierre, C., van der Meel, R., Kiessling, F. & Lammers, T. (2021).
Roadmap on nanomedicine.
Nanotechnology,
32(1), Article 012001.
https://doi.org/10.1088/1361-6528/abaadb
de Paz, L.
, Resin, A., Howard, K., Sutherland, D. S. & Wejse, P. (2011).
Antimicrobial effect of chitosan nanoparticles on streptococcus mutans biofilms.
Applied and Environmental Microbiology,
77(11), 3892-3895.
Dinesen, A., Winther, A., Wall, A.
, Märcher, A., Palmfeldt, J., Chudasama, V., Wengel, J.
, Gothelf, K. V., Baker, J. R.
& Howard, K. A. (2022).
Albumin Biomolecular Drug Designs Stabilized through Improved Thiol Conjugation and a Modular Locked Nucleic Acid Functionalized Assembly.
Bioconjugate Chemistry,
33(2), 333-342.
https://doi.org/10.1021/acs.bioconjchem.1c00561
Dinesen, A., Winther, A., Wall, A.
, Märcher, A., Palmfeldt, J., Chudasama, V., Wengel, J.
, Gothelf, K. V., Baker, J. R.
& Howard, K. (2022).
An Albumin Biomolecular Drug Design Stabilized through Improved Thiol Conjugation and a Modular Locked Nucleic Acid Assembly. Poster session presented at Controlled Release Society 2022 Annual Meeting, Montreal, Quebec, Canada.
Dinesen, A., Andersen, V. L., Elkhashab, M., Pilati, D., Bech, P., Fuchs, E., Samuelsen, T. R., Winther, A., Cai, Y., Märcher, A., Wall, A.
, Omer, M., Nielsen, J. S., Chudasama, V., Baker, J. R.
, Gothelf, K. V., Wengel, J.
, Kjems, J. & Howard, K. A. (2024).
An Albumin-Holliday Junction Biomolecular Modular Design for Programmable Multifunctionality and Prolonged Circulation.
Bioconjugate Chemistry,
35(2), 214-222.
https://doi.org/10.1021/acs.bioconjchem.3c00491
Dong, M. D., Howard, K. A., Oupicky, D., Bisht, H.
, Kjems, J. & Besenbacher, F. (2007).
Visualization of thermally activated nanocarriers using in situ atomic force microscopy: Nanotechnology.
Nanotechnology,
18(18), Article 185501.
https://doi.org/10.1088/0957-4484/18/18/185501
Ebbesen, M. F., Schaffert, D. H., Crowley, M. L., Oupický, D.
& Howard, K. (2013).
Synthesis of click-reactive HPMA copolymers using RAFT polymerization for drug delivery applications.
Journal of Polymer Science. Part A, Polymer Chemistry,
51(23), 5091-5099.
https://doi.org/10.1002/pola.26941
Ebbesen, M. F., Whitehead, B. J., Gonzalez, B. B., Kingshott, P.
& Howard, K. (2013).
Surface analysis of PEGylated nano-shields on nanoparticles installed by hydrophobic anchors.
Pharmaceutical Research,
30(7), 1758-1767.
https://doi.org/10.1007/s11095-013-1018-3
Ebbesen, M. F., Bienk, K., Deleuran, B. W. & Howard, K. (2014).
Extended circulation and accumulation of p(HPMA-co-AzMA) and accumulation in arthritic joints. Poster session presented at 41st Annual Meeting & Exposition of the Controlled Release Society, July 13-16, 2014 Chicago, USA , Chicago, United States.
Ebbesen, M. F., Olesen, M. T. J., Gjelstrup, M. C., Pakula, M. M., Larsen, E. K., Hansen, I. M., Hansen, P. L., Mollenhauer, J., Malle, B. M.
& Howard, K. (2015).
Tunable CD44-Specific Cellular Retargeting with Hyaluronic Acid Nanoshells.
Pharmaceutical Research,
32(4), 1462-1474.
https://doi.org/10.1007/s11095-014-1552-7
Elkhashab, M., Dilek, Y.
, Foss, M., Creemers, L. B.
& Howard, K. A. (2024).
A Modular Albumin-Oligonucleotide Biomolecular Assembly for Delivery of Antisense Therapeutics: Molecular Pharmaceutics.
Molecular Pharmaceutics,
21(2), 491-500.
https://doi.org/10.1021/acs.molpharmaceut.3c00561
Elvin, S. J., Eyles, J. E.
, Howard, K. A., Ravichandran, E., Somavarappu, S., Alpar, H. O. & Williamson, E. D. (2006).
Protection against bubonic and pneumonic plague with a single dose microencapsulated sub-unit vaccine.
Vaccine,
24(20), 4433-4439.
https://doi.org/10.1016/j.vaccine.2005.12.016
Fredsøe, J., Rasmussen, A. K. I.
, Laursen, E. B., Cai, Y., Howard, K. A., Pedersen, B. G., Borre, M., Mouritzen, P.
, Òrntoft, T. & Sørensen, K. D. (2019).
Independent Validation of a Diagnostic Noninvasive 3-MicroRNA Ratio Model (uCaP) for Prostate Cancer in Cell-Free Urine.
Clinical Chemistry,
65(4), 540-548.
https://doi.org/10.1373/clinchem.2018.296681
Fuchs, E., Rudnik-Jansen, I., Dinesen, A., Selnihhin, D., Mandrup, O. A., Thiam, K.
, Kjems, J., Pedersen, F. S. & Howard, K. A. (2022).
An albumin-angiotensin converting enzyme 2-based SARS-CoV-2 decoy with FcRn-driven half-life extension.
Acta Biomaterialia,
153, 411-418.
https://doi.org/10.1016/j.actbio.2022.09.048
Gaglianone, N., Hvam, M. L., Aslan, H., Dong, M., Howard, K. & Ho, Y.-P. (2016).
Chip-Free Microscale-Incubator-Based Synthesis of Chitosan-Based Gene Silencing Nanoparticles.
Particle & Particle Systems Characterization,
33(5), 279-285.
https://doi.org/10.1002/ppsc.201500225
Gao, S., Dagnæs-Hansen, F., Nielsen, E. J. B., Wengel, J.
, Besenbacher, F., Howard, K. A. & Kjems, J. (2009).
The effect of chemical modification and nanoparticle formulation on stability and biodistribution of siRNA in mice.
Molecular Therapy,
17(7), 1225-1233.
https://doi.org/10.1038/mt.2009.91
Garcia, JP., Stein, J.
, Cai, Y., Riemers, F., Wexselblatt, E., Wengel, J., Tryfonidou, M., Yayon, A.
, Howard, K. & Creemers, L. B. (2019).
Fibrin-hyaluronic acid hydrogel-based delivery of antisense oligonucleotides for ADAMTS5 inhibition in co-delivered and resident joint cells in osteoarthritis. Journal of Controlled Release,
294, 247-258.
https://doi.org/10.1016/j.jconrel.2018.12.030
Glud, S. Z., Bramsen, J. B., Dagnaes-Hansen, F., Wengel, J.
, Howard, K. A., Nyengaard, J. R. & Kjems, J. (2009).
Naked siLNA-mediated gene silencing of lung bronchoepithelium EGFP expression after intravenous administration.
Oligonucleotides,
19(2), 163-8.
https://doi.org/10.1089/oli.2008.0175
Glud, E. N., Rasmussen, M., Zhang, Y., Mandrup, O. A., Salachan, P. V.
, Borre, M., Sørensen, K. D. & Howard, K. A. (2022).
Identification of a high-risk immunogenic prostate cancer patient subset as candidates for T-cell engager immunotherapy and the introduction of a novel albumin-fused anti-CD3 × anti-PSMA bispecific design.
British Journal of Cancer,
127(12), 2186-2197.
https://doi.org/10.1038/s41416-022-01994-1
Gonzalez, B. B., Dagnæs-Hansen, F., Fenton, R. A., Gao, S., Hein, S., Dong, M., Kjems, J. & Howard, K. A. (2013).
Protection and systemic translocation of siRNA following oral administration of chitosan/siRNA nanoparticles.
Molecular Therapy - Nucleic Acids,
2(1), 1-8. Article e76.
https://doi.org/10.1038/mtna.2013.2
Hall, A., Nicholls , K., Caspersen , M. B.
, Skrivergaard, S., Howard, K., Karu, K., Chudasama, V. & Baker, J. R. (2019).
Optimised approach to albumin-drug conjugates using monobromomaleimide-C-2 linkers.
Organic & Biomolecular Chemistry,
17(34), 7870-7873.
https://doi.org/10.1039/C9OB00721K
Hangiu, O., Compte, M.
, Dinesen, A., Navarro, R., Tapia-Galisteo, A.
, Mandrup, O. A., Erce-Llamazares, A., Lázaro-Gorines, R., Nehme-Álvarez, D., Domínguez-Alonso, C.
, Harwood, S. L., Alfonso, C., Blanco, B., Rubio-Pérez, L., Jiménez-Reinoso, A., Díez-Alonso, L., Blanco, F. J., Sanz, L.
, Howard, K. A. & Álvarez-Vallina, L. (2022).
Tumor targeted 4-1BB agonist antibody-albumin fusions with high affinity to FcRn induce anti-tumor immunity without toxicity.
iScience,
25(9), Article 104958.
https://doi.org/10.1016/j.isci.2022.104958
Hansen, I., Ebbesen, M. F., Kaspersen, L., Thomsen, T. B., Bienk, K., Cai, Y., Malle, B. M.
& Howard, K. (2017).
Hyaluronic Acid Molecular Weight-Dependent Modulation of Mucin Nanostructure for Potential Mucosal Therapeutic Applications.
Molecular Pharmaceutics,
14(7), 2359–2367.
https://doi.org/10.1021/acs.molpharmaceut.7b00236
Hartmann, H., Lakner, U.
, Andersen, M. Ø., Kjems, J., Howard, K. A. & Schloßhauer, B. (2009).
Carrier Matrix for siRNA-Mediated Gene Knock Down to Promote Neuronal Regeneration. Tissue Engineering. Part A. Tissue Engineering, (3), 718.
Howard, K. A., Dong, M., Oupicky, D., Bisht, H. S., Buss, C.
, Besenbacher, F. & Kjems, J. (2007).
Nanocarrier stimuli-activated gene delivery.
Small,
3(1), 54-7.
https://doi.org/10.1002/smll.200600328
Howard, K. A., Paludan, S. R., Behlke, M. A.
, Besenbacher, F., Deleuran, B. W. & Kjems, J. (2008).
Chitosan/siRNA nanoparticle-mediated TNF-alpha knockdown in peritoneal macrophages for anti-inflammatory treatment in a murine arthritis model.
Molecular Therapy,
17(1), 162-168.
https://doi.org/10.1038/mt.2008.220
Howard, K. A., Rahbek, U. L., Liu, X., Damgaard, C. K., Glud, S. Z., Andersen, M. Ø., Hovgaard, M. B., Schmitz, A., Nyengaard, J. R., Besenbacher, F. & Kjems, J. (2006).
RNA interference in Vitro and in Vivo using a novel chitosan/siRNA nanoparticle system.
Molecular Therapy,
14(4), 476-484.
https://doi.org/10.1016/j.ymthe.2006.04.010