Research

There are three main focus areas of the lab

a) The understanding of how small non-coding RNA and circular RNA contribute to cell maintenance and disease development with a primary aim of defining new targets for disease intervention.

picture of heatmap of non-coding RNA elements under investigation as biomarkers for disease investigation
Non-coding RNA Biomarker development for disease targeting
picture of nucleic acid oligo used for drug design
Nucleic acid based drug design

b) The creation of novel bioimaging and delivery systems for gene medicine including siRNA, miRNA mimics, antimiRs (antisense targeting microRNA) with a specific focus on inflammation, cancer, influenza, and regeneration of damaged tissue (tissue engineering).

Picture of a mouse seen in optical and magnetic resonance bioimaging
Optical and magnetic resonance bioimaging
picture of a nanoparticle used for drug delivery
Nanoparticle delivery
picture of a 3d printer and scaffolds made by this printer
Implants, 3D printing, scaffolds, and tissue regeneration

c) Design and construction of functionalized self assembled DNA and RNA nanostructures capable of complex biosensing, coupled with controlled action e.g. drug release, enzyme activation, and receptor signaling. 

picture of how to do dna origami, nucleic acid nanostructures and cellular interphasing
DNA origami, nucleic acid nanostructures, and cellular interphasing

To accomplish the above we use a lot of different techniques, for instance:

  •  Deep sequencing for RNA profiling
  •  Photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP)
  •  RNA bioinformatics
  •  Aptamer selection
  •  Fluorescence microscopy
  •  DNA and RNA origami techniques
  •  Ensemble and single molecule FRET
  •  3D in vivo optical imaging
  •  siRNA delivery techniques
  •  Conjugation chemistry (e.g. Click Chemistry)
  •  High Throughput Q-PCR
  •  Fluorescence-activated cell analysis
  •  RNA and DNA biochemistry

Research Networks

International:

  • EU-FP7 Innovative anti-influenza drugs excluding viral escape (ANTIFLU) (2011-2016)
  • EU-FP7 MicroRNAs in the Pathogenesis, Treatment and Prevention of Epilepsy (EpimiRNA) (2013-2018)

National:

  • Jørgen Kjems is the director of Interdisciplinary Nanoscience Center (iNANO) (2002–  )
  • Jørgen Kjems is head of Lundbeck Nanomedicine center: Individualized management of tissue damage and regeneration “LUNA”  (2010-2015)
  • The Danish National Research Foundation: The Center for DNA Nanotechnology (CDNA). Gothelf, K. V., Besenbacher, F., Kjems, J., Ferapontova, E. & Birkedal, V. (01/03/12 → 28/02/16)
  • Jørgen Kjems is head of NABIIT strategic research center “NANOBAR” (2011-2015)
  • Partner of Aarhus University Centre for Integrative Sequencing  “iSEQ”  (2012-    )
  • Encapsulation of antimicrobial essential oils. Nielsen, C. K., Kjems, J., Mygind, T. & Meyer, R. L. (01/08/12 → 31/07/16)