DANDRITE Master Class: Principles and Practices of Phase Plate Cryo-Microscopy
Associate professor Elizabeth R. Wright, EMORY University School of Medicine, Atlanta
Info about event
Time
Location
iNANO meeting room 1590-213, Gustav Wieds Vej 14, 8000 Aarhus C
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DANDRITE Master Class: Principles and Practices of Phase Plate Cryo-Microscopy
Cryo-electron microscopy (cryo-EM) is undergoing a technological revolution that has moved the field from sub-nanometer resolution ‘blobs’ to atomic resolution structures. However, there remain challenges associated with imaging low contrast, radiation sensitive cryo-preserved specimens. ‘Enhanced’ phase contrast (PC) transmission electron microscopy (TEM) methods were developed to overcome these complications. I will present the theory behind the technologies and results showing the success of using phase plate methods for cryo-EM investigations of biological complexes.
Host: Professor Poul Nissen, DANDRITE & Dept. of Molecular Biology and Genetics, Aarhus University