Elkhashab, M, Dilek, Y
, Foss, M, Creemers, LB
& Howard, KA 2024, '
A Modular Albumin-Oligonucleotide Biomolecular Assembly for Delivery of Antisense Therapeutics: Molecular Pharmaceutics',
Molecular Pharmaceutics, vol. 21, no. 2, pp. 491-500.
https://doi.org/10.1021/acs.molpharmaceut.3c00561
Dinesen, A, Andersen, VL, Elkhashab, M, Pilati, D, Bech, P, Fuchs, E, Samuelsen, TR, Winther, A, Cai, Y, Märcher, A, Wall, A
, Omer, M, Nielsen, JS, Chudasama, V, Baker, JR
, Gothelf, KV, Wengel, J
, Kjems, J & Howard, KA 2024, '
An Albumin-Holliday Junction Biomolecular Modular Design for Programmable Multifunctionality and Prolonged Circulation',
Bioconjugate Chemistry, vol. 35, no. 2, pp. 214-222.
https://doi.org/10.1021/acs.bioconjchem.3c00491
Dinesen, A, Winther, A, Wall, A
, Märcher, A, Palmfeldt, J, Chudasama, V, Wengel, J
, Gothelf, KV, Baker, JR
& Howard, KA 2022, '
Albumin Biomolecular Drug Designs Stabilized through Improved Thiol Conjugation and a Modular Locked Nucleic Acid Functionalized Assembly',
Bioconjugate Chemistry, vol. 33, no. 2, pp. 333-342.
https://doi.org/10.1021/acs.bioconjchem.1c00561
Fuchs, E, Rudnik-Jansen, I, Dinesen, A, Selnihhin, D, Mandrup, OA, Thiam, K
, Kjems, J, Pedersen, FS & Howard, KA 2022, '
An albumin-angiotensin converting enzyme 2-based SARS-CoV-2 decoy with FcRn-driven half-life extension',
Acta Biomaterialia, vol. 153, pp. 411-418.
https://doi.org/10.1016/j.actbio.2022.09.048
Dinesen, A, Winther, A, Wall, A
, Märcher, A, Palmfeldt, J, Chudasama, V, Wengel, J
, Gothelf, KV, Baker, JR
& Howard, K 2022, '
An Albumin Biomolecular Drug Design Stabilized through Improved Thiol Conjugation and a Modular Locked Nucleic Acid Assembly', Controlled Release Society 2022 Annual Meeting, Montreal, Canada,
11/07/2022 -
15/07/2022.
Østergaard, RH, Yan, Y, Venø, MT
, Dinesen, A, Kjaer-Sorensen, K, Oxvig, C, Rasmussen, JT, Howard, K, Kjems, J, van Niel, G, Verweij, F
& Hayashi, Y 2022, '
Extracellular Vesicle Biology Meets Zebrafish: Real-Time Visualization and RNA Profiling of Physiological Exosomes in the Bloodstream', The Danish Society for Extracellular Vesicles (DSEV) 3rd Annual Symposium 2022, Roskilde, Denmark,
06/10/2022 -
06/10/2022.
Märcher, A, Kumar, V, Andersen, VL, El-Chami, K, Nguyen, TJD, Skaanning, MK, Rudnik-Jansen, I, Nielsen, JS, Howard, KA, Kjems, J & Gothelf, KV 2022, '
Functionalized Acyclic (l)-Threoninol Nucleic Acid Four-Way Junction with High Stability In Vitro and In Vivo',
Angewandte Chemie - International Edition, vol. 61, no. 24, 202115275.
https://doi.org/10.1002/anie.202115275
Glud, EN, Rasmussen, M, Zhang, Y, Mandrup, OA, Salachan, PV, Borre, M, Sørensen, KD & Howard, KA 2022, '
Identification of a high-risk immunogenic prostate cancer patient subset as candidates for T-cell engager immunotherapy and the introduction of a novel albumin-fused anti-CD3 × anti-PSMA bispecific design',
British Journal of Cancer, vol. 127, no. 12, pp. 2186-2197.
https://doi.org/10.1038/s41416-022-01994-1
Symons, RA, Colella, F, Collins, FL, Rafipay, AJ, Kania, K, McClure, JJ, White, N, Cunningham, I, Ashraf, S, Hay, E, Mackenzie, KS
, Howard, KA, Riemen, AHK, Manzo, A, Clark, SM, Roelofs, AJ & De Bari, C 2022, '
Targeting the IL-6-Yap-Snail signalling axis in synovial fibroblasts ameliorates inflammatory arthritis',
Annals of the Rheumatic Diseases, vol. 81, no. 2, pp. 214-224.
https://doi.org/10.1136/annrheumdis-2021-220875Hangiu, O, Compte, M
, Dinesen, A, Navarro, R, Tapia-Galisteo, A
, Mandrup, OA, Erce-Llamazares, A, Lázaro-Gorines, R, Nehme-Álvarez, D, Domínguez-Alonso, C
, Harwood, SL, Alfonso, C, Blanco, B, Rubio-Pérez, L, Jiménez-Reinoso, A, Díez-Alonso, L, Blanco, FJ, Sanz, L
, Howard, KA & Álvarez-Vallina, L 2022, '
Tumor targeted 4-1BB agonist antibody-albumin fusions with high affinity to FcRn induce anti-tumor immunity without toxicity',
iScience, vol. 25, no. 9, 104958.
https://doi.org/10.1016/j.isci.2022.104958Howard, K 2021,
Biomolecular Assemblies as Multifunctional Drug Designs. in
Handbook of Harnessing Biomaterials in Nanomedicine: Preparation, Toxicity, and Applications. 2 edn, Jenny Stanford Publishing, Singapore, Handbook of Harnessing Biomaterials in Nanomedicine: Preparation, Toxicity, and Applications. , pp. 363-380.
Walther, R, Monge, P, Pedersen, A, Benderoth, A, Pedersen, J, Farzadfard, A, Mandrup, O, Howard, K, Otzen, D & Zelikin, AN 2021, '
Per-glycosylation of the Surface-Accessible Lysines: One-Pot Aqueous Route to Stabilized Proteins with Native Activity',
ChemBioChem, vol. 22, no. 14, pp. 2478-2485.
https://doi.org/10.1002/cbic.202100228
Mandrup, OA, Ong, SC, Lykkemark, S, Dinesen, A, Rudnik-Jansen, I, Dagnæs-Hansen, NF, Andersen, JT, Alvarez-Vallina, L
& Howard, KA 2021, '
Programmable half-life and anti-tumour effects of bispecific T-cell engager-albumin fusions with tuned FcRn affinity',
Communications Biology, vol. 4, no. 1, 310.
https://doi.org/10.1038/s42003-021-01790-2
Decuzzi, P, Peer, D, Di Mascolo, D, Palange, AL, Manghnani, PN, Moein Moghimi, S, Shadi Farhangrazi, Z
, Howard, KA, Rosenblum, D, Liang, T, Chen, Z, Wang, Z, Zhu, JJ, Gu, Z, Korin, N, Letourneur, D, Chauvierre, C, van der Meel, R, Kiessling, F & Lammers, T 2021, '
Roadmap on nanomedicine',
Nanotechnology, vol. 32, no. 1, 012001.
https://doi.org/10.1088/1361-6528/abaadbCai, Y, Lou, C, Wengel, J
& Howard, KA 2020,
Albumin-Binding Fatty Acid-Modified Gapmer Antisense Oligonucleotides for Modulation of Pharmacokinetics. in T Yokota & R Maruyama (eds),
Gapmers : Methods and Protocols. Humana Press, Methods in Molecular Biology, vol. 2176, pp. 163-174.
https://doi.org/10.1007/978-1-0716-0771-8_12
Larsen, MT, Mandrup, OA, Schelde, KK, Luo, Y, Sørensen, KD, Dagnæs-Hansen, F, Cameron, J
, Stougaard, M, Steiniche, T & Howard, KA 2020, '
FcRn overexpression in human cancer drives albumin recycling and cell growth: a mechanistic basis for exploitation in targeted albumin-drug designs',
Journal of Controlled Release, vol. 322, pp. 53-63.
https://doi.org/10.1016/j.jconrel.2020.03.004
Kania, K, Colella, F, Riemen, AHK, Wang, H
, Howard, K, Aigner, T, Dell’Accio, F, Capellini, TD, Roelofs, AJ & De Bari, C 2020, '
Regulation of Gdf5 expression in joint remodelling, repair and osteoarthritis',
Scientific Reports, vol. 10, no. 1, 157, pp. 157.
https://doi.org/10.1038/s41598-019-57011-8Juul-Madsen, K, Qvist, P, Bendtsen, KL, Langkilde, AE, Vestergaard, B
, Howard, K, Dehesa-Etxebeste, M
, Paludan, SR, Andersen, GR, Jensen, PH, Otzen, DE, Romero-Ramos, M & Vorup-Jensen, T 2020, '
Size-Selective Phagocytic Clearance of Fibrillar α-Synuclein through Conformational Activation of Complement Receptor 4',
Journal of Immunology, vol. 204, no. 5, pp. 1345-1361.
https://doi.org/10.4049/jimmunol.1900494
Schelde, KK, Nicholls , K
, Dagnæs-Hansen, F, Bunting , K, Rawsthorne, H, Andersen, B, Finnis, C, Williamson, M, Cameron, J
& Howard, K 2019, '
A new class of recombinant human albumin with multiple surface thiols exhibit stable conjugation, and enhanced FcRn binding and blood circulation',
Journal of Biological Chemistry, vol. 294, no. 10, pp. 3735-3743.
https://doi.org/10.1074/jbc.RA118.005870
Garcia, JP, Stein, J
, Cai, Y, Riemers, F, Wexselblatt, E, Wengel, J, Tryfonidou, M, Yayon, A
, Howard, K & Creemers, LB 2019, '
Fibrin-hyaluronic acid hydrogel-based delivery of antisense oligonucleotides for ADAMTS5 inhibition in co-delivered and resident joint cells in osteoarthritis.',
Journal of Controlled Release, vol. 294, pp. 247-258.
https://doi.org/10.1016/j.jconrel.2018.12.030Fredsøe, J, Rasmussen, AKI
, Laursen, EB, Cai, Y, Howard, KA, Pedersen, BG, Borre, M, Mouritzen, P
, Òrntoft, T & Sørensen, KD 2019, '
Independent Validation of a Diagnostic Noninvasive 3-MicroRNA Ratio Model (uCaP) for Prostate Cancer in Cell-Free Urine',
Clinical Chemistry, vol. 65, no. 4, pp. 540-548.
https://doi.org/10.1373/clinchem.2018.296681
Nandakumar, R, Tschismarov, R, Meissner, F
, Prabakaran, T, Krissanaprasit, A, Farahani, E, Zhang, BC, Assil, S, Martin, A, Bertrams, W
, Holm, CK, Ablasser, A
, Klause, T, Thomsen, MK, Schmeck, B
, Howard, KA, Henry, T
, Gothelf, KV, Decker, T
& Paludan, SR 2019, '
Intracellular bacteria engage a STING–TBK1–MVB12b pathway to enable paracrine cGAS–STING signalling',
Nature Microbiology, vol. 4, pp. 701-713.
https://doi.org/10.1038/s41564-019-0367-z
Hall, A, Nicholls , K, Caspersen , MB
, Skrivergaard, S, Howard, K, Karu, K, Chudasama, V & Baker, JR 2019, '
Optimised approach to albumin-drug conjugates using monobromomaleimide-C-2 linkers',
Organic & Biomolecular Chemistry, vol. 17, no. 34, pp. 7870-7873.
https://doi.org/10.1039/C9OB00721KLarsen, MT, Rawsthorne, H
, Schelde, KK, Dagnæs-Hansen, F, Cameron, J
& Howard, K 2018, '
Cellular recycling-driven in vivo half-life extension using recombinant albumin fusions tuned for neonatal Fc receptor (FcRn) engagement',
Journal of Controlled Release, vol. 287, pp. 132-141.
https://doi.org/10.1016/j.jconrel.2018.07.023
Cai, Y, Makarova, A-M, Wengel, J
& Howard, K 2018, '
Palmitoylated phosphodiester gapmer designs with albumin binding capacity and maintained in vitro gene silencing activity',
Journal of Gene Medicine, vol. 20, no. 7-8, e3025.
https://doi.org/10.1002/jgm.3025
Cai, Y, López-Ruiz , E, Wengel, J, Creemers, L
& Howard, K 2017, '
A hyaluronic acid-based hydrogel enabling CD44-mediated chondrocyte binding and gapmer oligonucleotide release for modulation of gene expression in osteoarthritis',
Journal of Controlled Release, vol. 253, no. May, pp. 153-159.
https://doi.org/10.1016/j.jconrel.2017.03.004
Caspersen , MB
, Kuhlmann, M, Nicholls , K, Saxton , MJ, Andersen , B, Bunting , K, Cameron, J
& Howard, K 2017, '
Albumin-based drug delivery using cysteine 34 chemical conjugates; important considerations and requirements',
Therapeutic Delivery, vol. 8, no. 7, pp. 511-519.
https://doi.org/10.4155/tde-2017-0038Kuhlmann, M, Hamming, JBR, Voldum, A, Tsakiridou, G, Larsen, MT, Schmøkel, J, Sohn, E, Bienk, K, Schaffert, DH, Sørensen, ES, Wengel, J
, Dupont, DM & Howard, K 2017, '
An albumin-oligonucleotide assembly for potential combinatorial drug delivery and half-life extension applications',
Molecular Therapy - Nucleic Acids, vol. 9, pp. 284-293.
https://doi.org/10.1016/j.omtn.2017.10.004
Schmidt, EGW
, Hvam, ML, Antunes, F, Cameron, J, Viuff, D, Andersen, B, Kristensen, NN
& Howard, KA 2017, '
Direct demonstration of a neonatal Fc receptor (FcRn)-driven endosomal sorting pathway for cellular recycling of albumin',
Journal of Biological Chemistry, vol. 292, no. 32, pp. 13312-13322.
https://doi.org/10.1074/jbc.M117.794248Hvam, ML, Cai, Y, Dagnæs-Hansen, F, Nielsen, J, Wengel, J
, Kjems, J & Howard, K 2017, '
Fatty acid-modified gapmer antisense oligonucleotide and human serum albumin constructs for pharmacokinetic modulation',
Molecular Therapy, vol. 25, no. 7, pp. 1710-1717.
https://doi.org/10.1016/j.ymthe.2017.05.009
Hansen, I, Ebbesen, MF, Kaspersen, L, Thomsen, TB, Bienk, K, Cai, Y, Malle, BM
& Howard, K 2017, '
Hyaluronic Acid Molecular Weight-Dependent Modulation of Mucin Nanostructure for Potential Mucosal Therapeutic Applications',
Molecular Pharmaceutics, vol. 14, no. 7, pp. 2359–2367.
https://doi.org/10.1021/acs.molpharmaceut.7b00236
Blans, KIM, Hansen, MS, Sørensen, LV
, Hvam, ML, Howard, K, Möller, A, Wiking, L, Larsen, LB & Rasmussen, JT 2017, '
Pellet-free isolation of human and bovine milk extracellular vesicles by size-exclusion chromatography',
Journal of Extracellular Vesicles, vol. 6, no. 1, 1294340.
https://doi.org/10.1080/20013078.2017.1294340
Schmøkel, J, Voldum, A, Tsakiridou, G, Kuhlmann, M, Cameron, J
, Sørensen, ES, Wengel, J
& Howard, K 2017, '
Site-selective conjugation of an anticoagulant aptamer to recombinant albumins and maintenance of neonatal Fc receptor binding',
Nanotechnology, vol. 28, no. 20, 204004 .
https://doi.org/10.1088/1361-6528/aa6a9b
Christiansen, SH, Murphy, RA
, Juul-Madsen, K, Fredborg, M, Hvam, ML, Axelgaard, E, Skovdal, SM, Meyer, RL, Sorensen, UBS, Moeller, A, Nyengaard, JR, Norskov-Lauritsen, N, Wang, M, Gadjeva, M
, Howard, KA, Davies, JC
, Petersen, E & Vorup-Jensen, T 2017, '
The Immunomodulatory Drug Glatiramer Acetate is Also an Effective Antimicrobial Agent that Kills Gram-negative Bacteria',
Scientific Reports, vol. 7, no. 1, 15653.
https://doi.org/10.1038/s41598-017-15969-3
Christiansen, SH, Zhang, X, Juul-Madsen, K, Hvam, ML, Vad, BS, Behrens, MA, Thygesen, IL, Jalilian, B, Pedersen, JS, Howard, K, Otzen, DE & Vorup-Jensen, T 2017, '
The random co-polymer glatiramer acetate rapidly kills primary human leukocytes through sialic-acid-dependent cell membrane damage',
B B A - Biomembranes, vol. 1859, no. 3, pp. 425-437.
https://doi.org/10.1016/j.bbamem.2017.01.001
Larsen, MT, Kuhlmann, M, Hvam, ML & Howard, K 2016, '
Albumin-based drug delivery: harnessing nature to cure disease',
Molecular and Cellular Therapies, vol. 4, no. 3, pp. 3.
https://doi.org/10.1186/s40591-016-0048-8
Bienk, K, Hvam, ML, Pakula, MM, Dagnæs-Hansen, F, Wengel, J, Malle, BM
, Kragh-Hansen, U, Cameron, J, Bukrinski, JT
& Howard, K 2016, '
An albumin-mediated cholesterol design-based strategy for tuning siRNA pharmacokinetics and gene silencing',
Journal of Controlled Release, vol. 232, pp. 143-151.
https://doi.org/10.1016/j.jconrel.2016.04.013
Gaglianone, N, Hvam, ML, Aslan, H, Dong, M, Howard, K & Ho, Y-P 2016, '
Chip-Free Microscale-Incubator-Based Synthesis of Chitosan-Based Gene Silencing Nanoparticles',
Particle & Particle Systems Characterization, vol. 33, no. 5, pp. 279-285.
https://doi.org/10.1002/ppsc.201500225
Viuff, D, Antunes, F, Evans, L, Cameron, J
, Dyrnesli, H, Ravn, BT
, Stougaard, M, Thiam, K, Andersen, B, Kjærulff, S
& Howard, K 2016, '
Generation of a Double Transgenic Humanised Neonatal Fc Receptor (FcRn)/Albumin Mouse to Study the Pharmacokinetics of Albumin-linked Drugs',
Journal of Controlled Release, vol. 223, pp. 22-30.
https://doi.org/10.1016/j.jconrel.2015.12.019Li, Z, Hu, Y, Chang, M
, Howard, K, Fan, X
, Sun, Y, Besenbacher, F & Yu, M 2016, '
Highly porous PEGylated Bi2S3 nano-urchins as a versatile platform for in vivo triple-modal imaging, photothermal therapy and drug delivery',
Nanoscale, vol. 8, no. 35, pp. 16005-16016.
https://doi.org/10.1039/C6NR03398A
Li, Z, Hu, Y, Jiang, T
, Howard, K, Li, Y, Fan, X, Sun, Y
, Besenbacher, F & Yu, M 2016, '
Human serum albumin coated Prussian blue nanoparticles as pH-/thermo-triggered drug delivery vehicles for cancer thermo-chemotherapy',
Particle & Particle Systems Characterization, vol. 33, no. 1, pp. 53-62.
https://doi.org/10.1002/ppsc.201500189
Martirosyan, A, Olesen, MJ, Fenton, RA, Kjems, J & Howard, KA 2016, '
Mucin-mediated nanocarrier disassembly for triggered uptake of oligonucleotides as a delivery strategy for the potential treatment of mucosal tumours',
Nanoscale, vol. 8, no. 25, pp. 12599-12607.
https://doi.org/10.1039/c5nr07206a
Li, Z, Hu, Y
, Howard, K, Yiang, T, Fan, X, Miao, Z, Sun, Y
, Besenbacher, F & Yu, M 2016, '
Multifunctional bismuth selenide nanocomposites for anti-tumor thermo-chemotherapy and imaging',
A C S Nano, vol. 10, no. 1, pp. 984-997.
https://doi.org/10.1021/acsnano.5b06259
Li, Z, Liu, J
, Howard, K, Fan, X, Chang, M, Sun, Y
, Besenbacher, F & Yu, M 2016, '
Multimodal Imaging-Guided Antitumor Photothermal Therapy and Drug Delivery Using Bismuth Selenide Spherical-Sponge',
A C S Nano, vol. 10, no. 10, pp. 9646–9658.
https://doi.org/10.1021/acsnano.6b05427Petersen, S, Kläning, E, Andersen, B, Cameron, J
, Sørensen, ES & Howard, K 2016, '
Neonatal Fc Receptor Binding Tolerance toward the Covalent Conjugation of Payloads to Cysteine 34 of Human Albumin Variants',
Molecular Pharmaceutics, vol. 13, no. 2, pp. 677–682.
https://doi.org/10.1021/acs.molpharmaceut.5b00605
