Specialized iNANO Lecture: Nanoprobes for Single-Cell Surgery
Ass. professor Paolo Actis, University of Leeds, UK
Info about event
Time
Location
iNANO meeting room 1590-213, Gustav Wieds Vej 14, 8000 Aarhus C
![](/fileadmin/news_import/Paolo_Actis.jpg)
Assistant professor Paolo Actis, University of Leeds, UK
Nanoprobes for Single-Cell Surgery
Physiological and pathological processes within the human body are controlled by complex cell-cell interactions within the context of a dynamic microenvironment. The ability to dynamically measure phenotypes (i.e. gene expression, protein activities, ion fluctuations, signalling) at the single cell level is key to understanding cellular behaviour in a complex environment. My research aims at developing nanoscale tools to dissect the dynamic contributions of single cells to disease processes.
In the first part of my talk, I will present the development of nanoprobes based on nanopipettes for manipulating living cells. The integration of these nanoprobes with scanning probe microscopy techniques allows the precise delivery of biomolecules1, 2 and the extraction of genetic material and organelles from living cells3.
In the second part of my talk, I will discuss the application of functionalised nanoelectrodes for intracellular electrochemical measurements. Nanoelectrodes as small as 3 nm in radius can be functionalized with platinum4 and Prussian blue5,6 to dramatically enhance their catalytic activity towards oxygen reduction and hydrogen peroxide oxidation. I will discuss their application for measuring the metabolic activity inside brain slices and I will conclude my talk showing their ability to measure intracellular endogenous molecules.
References
- Seger, Actis et al, Nanoscale, 2012
- Ivanov, Actis et al, ACS Nano, 2015
- Actis et al, ACS Nano, 2013
- Actis et al, ACS Nano, 2014
- Clausmeyer, Actis et al, Electrochem Commun, 2014
- Marquitan et al, Chem Electro Chem, 2016
Host: Assoc. professor Mingdong Dong, Interdisciplinary Nanoscience Center, Aarhus University