Dr. Rafael Fernández Leiro, PhD Medical Research Council - Laboratory of Molecular Biology Cambridge Medical Campus,  United Kingdom

Single-particle analysis using RELION

New advances in cryo-electron microscopy (cryo-EM) have opened the window to near atomic resolution structures not only on large macromolecules but also on much smaller and challenging biological systems. Thanks to the development of new methods that can deal with compositional and conformational heterogeneity also transient and highly dynamic complexes can now be studied. We will cover the essential steps during single-particle cryo-EM analysis with emphasis in the challenges and opportunities arising from structural heterogeneity.

Host: Thomas Boesen, Cryo-EM facility manager, iNANO & Department of Molecular Biology and Genetics, Aarhus University