Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Dalgaard, E , Bredesen, K , Mathiesen, A & Balling, N 2019, 'De-risking geothermal plays by seismic reservoir characterisation', Paper presented at 1st Conference on Geophysics for Geothermal-Energy Utilization and Renewable-Energy Storage, Held at Near Surface Geoscience Conference and Exhibition 2019, NSG 2019, The Hague, Netherlands, 08/09/2019 - 12/09/2019.

Szijgyarto, Z, Rampat, R, Werner, GS, Ho, C, Reifart, N, Lefevre, T, Louvard, Y, Avran, A, Kambis, M, Buettner, HJ, Di Mario, C, Gershlick, A, Escaned, J, Sianos, G, Galassi, A, Garbo, R, Goktekin, O, Meyer-Gessner, M, Lauer, B, Elhadad, S, Bufe, A, Boudou, N, Sievert, H, Martin-Yuste, V, Thuesen, L, Erglis, A, Christiansen, E, Spratt, J, Bryniarski, L, Clayton, T & Hildick-Smith, D 2019, 'Derivation and Validation of a Chronic Total Coronary Occlusion Intervention Procedural Success Score From the 20,000-Patient EuroCTO Registry: The EuroCTO (CASTLE) Score', JACC: Cardiovascular Interventions, vol. 12, no. 4, pp. 335-342. https://doi.org/10.1016/j.jcin.2018.11.020

Schmidtmann, C, Kargo, M , Ettema, JF, Hinrichs, D & Thaller, G 2019, 'Derivation of economic values for German dairy breeds', 70th Annual Meeting of the European Federation of Animal Science EAAP 2019, Ghent, Belgium, Ghent, Belgium, 26/08/2019 - 30/08/2019 pp. 609-609.

Blömer, M 2019, Der Mons Argaios und andere göttliche Berge in römischer Zeit. in Natur und Kult in Anatolien . Deutschen Archäologischen Institut, Istanbul, pp. 253-282.

Christensen, TP, Martinsen, B, Pedersen, BH, Jensen, BB & Bisgaard, O 2019, 'Der skal tænkes nyt på tolkeområdet – rets- og patientsikkerheden er i spil', Morgensavisen Jyllands-Posten. <https://jyllands-posten.dk/debat/breve/ECE11732347/der-skal-taenkes-nyt-paa-tolkeomraadet-rets-og-patientsikkerheden-er-i-spil/>

Stegmann, R & Schneuwly-Purdie, M 2019, Der Umgang mit dem Erbe: Positionen von Muslimen und Musliminnen in der Schweiz. <https://doc.rero.ch/record/327528/files/a5_szig_papers6_d_web_final.pdf>

Fauth, SR 2019, '"Der Weltwanderer" und "der Hinhocker". Heimkehr- und Welteroberungsmotive in Wilhelm Raabes »Stopfkuchen«', 26. Deutscher Germanistentag 2019 – Zeit, Saarbrücken, Germany, 23/09/2019 - 25/09/2019.

Kristensen, HK 2019, Der Wiederaufbau Viborgs nach dem Brand von 1726 – mit Hilfe von deutschen Baumeistern. in Dänemark und der Hausbau im Norden. Michael Imhof Verlag, Jahrbuch für Hausforschung, vol. 64, pp. 131-144.

Skiveren, T 2019, 'De sære, de enkle og de talende. Sådan ser dansk lyrik ud i efteråret 2019', Jyllands-Posten, vol. Bøger, pp. 26.

Suhr, C 2019, Descending with angels: islamic exorcism and psychiatry: a film monograph. Manchester University Press.

Deckers, PS & Nolet, K 2019, 'De schittering van het nieuwe geloof. Een vliegende start voor Medea', Ex situ. Tijdschrift voor Vlaamse Archeologie, vol. 24, pp. 30-31.

Ratner, HG 2019, 'Describing Children at Risk: Experiments with Context ', Discourse: Studies in the Cultural Politics of Education, vol. 40, no. 1, pp. 16-28. https://doi.org/10.1080/01596306.2018.1549701

Poier, PP & Jensen, F 2019, 'Describing Molecular Polarizability by a Bond Capacity Model', Journal of Chemical Theory and Computation, vol. 15, no. 5, pp. 3093-3107. https://doi.org/10.1021/acs.jctc.8b01215

Griffiths, ET, McCullough, J, Barlow, J & Moore, JE 2019, 'Description of a new beaked whale echolocation pulse type in the California Current', Marine Mammal Science, vol. 35, no. 3, pp. 1058-1069. https://doi.org/10.1111/mms.12560

Ljungberg, T & Kristiansen, SM 2019, De seneste årtusinders havniveau ved Nørholm i den centrale del af Limfjorden. Forlaget Odense Bys Museer. <https://museum.odense.dk/media/13275310/Rapport-6_opsat.pdf>

Costa, C, Svystun, O , Wenzel, A, Carneiro, AL, Zambrana, JRM, MIranda Richarte de Andrade Salgado, D & Spin-Neto, R 2019, 'Desenvolvimento de programa personalizado (macro) para realização de análise cefalométrica utilizando software aberto (open source)', Brazilian Oral Research, vol. 33, no. Supplement 2, AO0181, pp. 62. <https://www.sbpqo.org.br/hotsite2019/SBPqO_BORv033_book_p5.pdf>

Bauer, SE, Im, U, Mezuman, K & Gao, CY 2019, 'Desert Dust, Industrialization, and Agricultural Fires: Health Impacts of Outdoor Air Pollution in Africa', Journal of Geophysical Research: Atmospheres, vol. 124, no. 7, pp. 4104-4120. https://doi.org/10.1029/2018JD029336

Olesen, T 2019, 'Deservingness and political preference in opinions on distant suffering', Current Sociology, vol. 67, no. 3, pp. 456-473. https://doi.org/10.1177/0011392118807536

Hänsel, A & Heck, M 2019, 'Design and analysis of an integrated widely tunable laser', European Conference on Integrated Optics 2019, Ghent, Belgium, 24/04/2019 - 26/04/2019.

Stewart, JM, Geary, C & Franco, E 2019, 'Design and characterization of RNA nanotubes', ACS Nano, vol. 13, no. 5, pp. 5214-5221. https://doi.org/10.1021/acsnano.8b09421

Jalil, AAMM, Faudzi Mat Isa, M, Rostani, K, Othman, NA, Shariff, AM, Lau, KK, Partoon, B & Tay, WH 2019, Design and engineering implementation of blowdown system for cryogenic CO2- NG pilot facility. in Proceedings - SPE Annual Technical Conference and Exhibition. Society of Petroleum Engineers, SPE Annual Technical Conference and Exhibition 2019, ATCE 2019, Calgary, Canada, 30/09/2019. https://doi.org/10.2118/196224-MS

Harikrishnan, H, Sarkar, A, Paolini, C & Mihovska, A 2019, Design and Evaluation of a novel MAC protocol for multi implantable UHF-RFID transmitters in Brain Computer Interface Applications. in 2019 Wireless Telecommunications Symposium, WTS 2019., 8715532, IEEE, 18th Annual Wireless Telecommunications Symposium, WTS 2019, New York City, United States, 09/04/2019. https://doi.org/10.1109/WTS.2019.8715532

Arabkoohsar, A & Andresen, GB 2019, 'Design and optimization of a novel system for trigeneration', Energy, vol. 168, pp. 247-260. https://doi.org/10.1016/j.energy.2018.11.086

Kiss, EL, Berkó, S, Gácsi, A, Kovács, A, Katona, G, Soós, J, Csányi, E, Gróf, I, Harazin, A, Deli, MA & Budai-Szűcs, M 2019, 'Design and optimization of nanostructured lipid carrier containing dexamethasone for ophthalmic use', Pharmaceutics, vol. 11, no. 12, 679. https://doi.org/10.3390/pharmaceutics11120679

Unnikrishnan, R, Deshmukh, A, Ramesh, S, Babu, SK, Anitha, P & Bhavani, RR 2019, Design and Perception of a Social Robot to Promote Hand Washing among Children in a Rural Indian School. in 2019 28th IEEE International Conference on Robot and Human Interactive Communication, RO-MAN 2019., 8956450, IEEE, 28th IEEE International Conference on Robot and Human Interactive Communication, RO-MAN 2019, New Delhi, India, 14/10/2019. https://doi.org/10.1109/RO-MAN46459.2019.8956450

Soprani, S, Marongiu, F, Christensen, L, Alm, O, Petersen, KD , Ulrich, T & Engelbrecht, K 2019, 'Design and testing of a horizontal rock bed for high temperature thermal energy storage', Applied Energy, vol. 251, 113345. https://doi.org/10.1016/j.apenergy.2019.113345

Valero Moreno, J, Centola, M, Ma, Y, Škugor, M, Yu, Z, Haydell, MW, Keppner, D & Famulok, M 2019, 'Design, assembly, characterization, and operation of double-stranded interlocked DNA nanostructures', Nature Protocols, vol. 14, no. 10, pp. 2818-2855. https://doi.org/10.1038/s41596-019-0198-7

Andersen, P 2019, Design din digitale transformation.

Carlson, DF , Melvad, C , Rysgaard, S , Vesterled, L, Skovby, M, Fürsterling, A & Pedersen, SS, Design files and LabVIEW VIs for the Arctic Research Center Autonomous Boat (ARCAB), 2019, Dataset, Elsevier, Mendeley. https://doi.org/10.17632/287wmj4mzj.1

Carlson, DF, Pasma, J, Jacobsen, M, Hansen, M, Thomsen, S, Lillethorup, J, Tirsgaard, F, Flytkjær, A & Melvad, C, Design files and LabVIEW VIs for the IceDrone, 2019, Dataset, Elsevier. <https://data.mendeley.com/datasets/jx7b5t4f6j/1>

Boer, H & Boer, H 2019, 'Design-for-variety and operational performance: The mediating role of internal, supplier and customer integration', Journal of Manufacturing Technology Management, vol. 30, no. 2, pp. 438-461. https://doi.org/10.1108/JMTM-03-2018-0065

Katibeh, M, Kalantarion, M, Sabbaghi, H, Mousavi, B, Schriver, M, Nikkhah, H, Ahmadieh, H & Kallestrup, P 2019, 'Designing a screening program for prevention of avoidable blindness in Iran through a participatory action approach', Journal of Ophthalmic and Vision Research, vol. 14, no. 1, pp. 52-61. https://doi.org/10.4103/jovr.jovr_43_18

Hammershøj, M 2019, 'Designing biofunctional dairy foods: Matrix structure of dairy products in relation to lipaemia', Dairy Research Conference 2019, Billund, Denmark, 27/03/2019 - 27/03/2019.

Yildiz, E, Melo, M, Møller, C & Bessa, M 2019, Designing Collaborative and Coordinated Virtual Reality Training Integrated with Virtual and Physical Factories. in 2019 International Conference on Graphics and Interaction (ICGI). IEEE, pp. 48-55, 2019 International Conference on Graphics and Interaction, Faro, Portugal, 21/11/2019. https://doi.org/10.1109/ICGI47575.2019.8955033

Tutenges, S & Bøhling, F 2019, 'Designing drunkenness: How pubs, bars and nightclubs increase alcohol sales', International Journal of Drug Policy, vol. 70, pp. 15-21. https://doi.org/10.1016/j.drugpo.2019.04.009

Niemantsverdriet, K, van Essen, H, Pakanen, MA & Eggen, B 2019, 'Designing for Awareness in Interactions with Shared Systems: The DASS Framework', ACM Transactions on Computer-Human Interaction, vol. 26, no. 6, 36. https://doi.org/10.1145/3338845

Hilli, C, Nørgård, RT & Aaen, JH 2019, 'Designing Hybrid Learning Spaces in Higher Education', Dansk Universitetspædagogisk Tidsskrift, vol. 15, no. 27, pp. 66-82. <https://dun-net.dk/media/944279/dut27_hilli_et_al_designing_hybrid_learning.pdf>

Van Mechelen, M 2019, Designing Internet of Toys for and with Children: A Participatory Design Case Study. in G Mascheroni & D Holloway (eds), The Internet of Toys: Practices, Affordances and the Political Economy of Children’s Smart Play. Palgrave Macmillan, Cham, Studies in Childhood and Youth, pp. 181-203. https://doi.org/10.1007/978-3-030-10898-4_9

Hasse, C & Søndergaard, DM (eds) 2019, Designing Robots, Designing Humans. Routledge.

Heiselberg, K 2019, Designing self-tracking devices for vulnerable chronic ill. in DIS 2019 Companion - Companion Publication of the 2019 ACM Designing Interactive Systems Conference. Association for Computing Machinery, pp. 89-92, 2019 ACM Conference on Designing Interactive Systems, DIS 2019, San Diego, United States, 23/06/2019. https://doi.org/10.1145/3301019.3324876

Parsons, CE , Jensen, KL , Roepstorff, A , Fjorback, LO & Linehan, C 2019, 'Designing technology tools to support engagement in mindfulness-based interventions: an analysis of teacher and student experiences', Digital Health, vol. 5. https://doi.org/10.1177/2055207619868550

Hussain, A, Chaniago, YD, Riaz, A & Lee, M 2019, 'Design method for the feasibility and technical evaluation of side-reactor column configurations', Chemical Engineering and Processing - Process Intensification, vol. 144, 107648. https://doi.org/10.1016/j.cep.2019.107648

Jaén, KE, Velázquez, D, Sigala, JC & Lara, AR 2019, 'Design of a microaerobically inducible replicon for high-yield plasmid DNA production', Biotechnology and Bioengineering, vol. 116, no. 10, pp. 2514-2525. https://doi.org/10.1002/bit.27091

Wijsenbeek, M, Bendstrup, E, Valenzuela, C, Henry, MT, Moor, C, Bengus, M, Perjesi, A, Gilberg, F, Kirchgaessler, K-U & Vancheri, C 2019, 'Design of a Study Assessing Disease Behaviour During the Peri-Diagnostic Period in Patients with Interstitial Lung Disease: The STARLINER Study', Advances in Therapy, vol. 36, no. 1, pp. 232-243. https://doi.org/10.1007/s12325-018-0845-3

Lara, AR, Velázquez, D, Penella, I, Islas, F, González-De la Rosa, CH & Sigala, JC 2019, 'Design of a synthetic miniR1 plasmid and its production by engineered Escherichia coli', Bioprocess and Biosystems Engineering, vol. 42, no. 8, pp. 1391-1397. https://doi.org/10.1007/s00449-019-02129-2

Jaspers, SØ, Jakobsen, LM, Gadegaard, CA, Dyreborg, J, Andersen, LPS & Aust, B 2019, 'Design of a tailored and integrated violence prevention program in psychiatric wards and prisons', Work, vol. 62, no. 4, pp. 525-541. https://doi.org/10.3233/WOR-192888

Knudsen, J , Sørensen, KK, Steenfelt, JS & Trankjær , H 2019, 'Design of bored piles in Denmark - a historical perspective', Paper presented at XVII European Conference on Soil Mechanics and Geotechnical Engineering, Reykjavik, Iceland, 01/09/2019 - 06/09/2019. https://doi.org/10.32075/17ECSMGE-2019-0732

Barbero, N, Abbiati, G, Enrico, E, Amato, G, Ott, HR, Mesot, J & Shiroka, T 2019, 'Design Optimization through Thermomechanical Finite-Element Analysis of a Hybrid Piston-Clamped Anvil Cell for Nuclear Magnetic Resonance Experiments', Review of Scientific Instruments, vol. 90, no. 1, 013901. https://doi.org/10.1063/1.5059391

Subrizi, A, del Amo, EM, Korzhikov-Vlakh, V, Tennikova, T, Ruponen, M & Urtti, A 2019, 'Design principles of ocular drug delivery systems: importance of drug payload, release rate, and material properties', Drug Discovery Today, vol. 24, no. 8, pp. 1446-1457. https://doi.org/10.1016/j.drudis.2019.02.001

Reick, P 2019, 'Desire or Displacement? Working-Class Notions of Urban Belonging in Late-Nineteenth-Century Germany', Journal of Urban History, vol. 45, no. 6, pp. 1193-1211. https://doi.org/10.1177/0096144218792503, https://doi.org/10.1177/0096144218792503

Displaying results 95651 to 95700 out of 389648

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Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen