Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Bach, L, Zbawicka, M, Strand, J & Wenne, R 2019, 'Mytilus trossulus in NW Greenland is genetically more similar to North Pacific than NW Atlantic populations of the species', Marine Biodiversity, vol. 49, no. 2, pp. 1053-1059. https://doi.org/10.1007/s12526-018-0870-0

Xiao, R, Liu, K, Bai, L, Minakata, D, Seo, Y, Kaya Göktaş, R, Dionysiou, DD, Tang, CJ, Wei, Z & Spinney, R 2019, 'Inactivation of pathogenic microorganisms by sulfate radical: Present and future', Chemical Engineering Journal, vol. 371, pp. 222-232. https://doi.org/10.1016/j.cej.2019.03.296

Ryom, K, Eshøj, S, Simonsen, CB, Nielsen, G, Troelsen, J & Maindal, HT 2019, Inaktivitet i Danmark, delrapport 1: Kortlægning af eksisterende viden om faktorer, der påvirker inaktivitet. Institut for Folkesundhed, Aarhus Universitet, Aarhus.

Hilbolling, S, Berends, H, Deken, F & Tuertscher, P 2019, 'In and out of sync: Temporal coordination in digital innovation ecosystems', Paper presented at 11th International Process Symposium , Chania, Greece, 19/06/2019 - 22/06/2019.

Lykkegaard, E & Ulriksen, L 2019, 'In and out of the STEM pipeline–a longitudinal study of a misleading metaphor', International Journal of Science Education, vol. 41, no. 12, pp. 1600-1625. https://doi.org/10.1080/09500693.2019.1622054

Larsen, SN 2019, '”I naturen kan man ikke score 12-taller”: Anmelder Rane Willerslev: "Rygrad og rummelighed. Et oplæg til et mere modigt dannelsesbegreb" (People’sPress) ', Information, pp. 26.

Bak-Nielsen, S , Ramlau-Hansen, CH , Ivarsen, A , Plana-Ripoll, O & Hjortdal, J 2019, 'Incidence and prevalence of keratoconus in Denmark - an update', Acta Ophthalmologica, vol. 97, no. 8, pp. 752-755. https://doi.org/10.1111/aos.14082

Furer, V, Rondaan, C, Heijstek, M, Van Assen, S, Bijl, M, Agmon-Levin, N, Breedveld, FC, D'Amelio, R, Dougados, M, Kapetanovic, MC, Van Laar, JM, Ladefoged De Thurah, A, Landewé, R, Molto, A, Müller-Ladner, U, Schreiber, K, Smolar, L, Walker, J, Warnatz, K, Wulffraat, NM & Elkayam, O 2019, 'Incidence and prevalence of vaccine preventable infections in adult patients with autoimmune inflammatory rheumatic diseases (AIIRD): A systemic literature review informing the 2019 update of the EULAR recommendations for vaccination in adult patients with AIIRD', RMD Open, vol. 5, no. 2, e001041. https://doi.org/10.1136/rmdopen-2019-001041

Petersen, PB, Kehlet, H, Jørgensen, CC, Lundbeck Foundation Center for Fast-track Hip and Knee Replacement Collaborative Group, Madsen, F , Hansen, TB, Husted, H, Laursen, MB, Hansen, LT, Kjærsgaard-Andersen, P, Solgaard, S & Krarup, NH 2019, 'Incidence and Risk Factors for Stroke in Fast-Track Hip and Knee Arthroplasty—A Clinical Registry Study of 24,862 Procedures', The Journal of Arthroplasty, vol. 34, no. 4, pp. 743-749.e2. https://doi.org/10.1016/j.arth.2018.12.022

Aminian, A, Schmidt, B, Mazzone, P, Berti, S, Fischer, S, Montorfano, M, Lam, SCC, Lund, J, Asch, FM, Gage, R, Cruz-Gonzalez, I, Omran, H, Tarantini, G & Nielsen-Kudsk, JE 2019, 'Incidence, Characterization, and Clinical Impact of Device-Related Thrombus Following Left Atrial Appendage Occlusion in the Prospective Global AMPLATZER Amulet Observational Study', JACC: Cardiovascular Interventions, vol. 12, no. 11, pp. 1003-1014. https://doi.org/10.1016/j.jcin.2019.02.003

Ruwald, MH, Ruwald, A-C, Johansen, JB, Gislason, G, Nielsen, JC, Philbert, B, Riahi, S, Vinther, M & Lindhardt, TB 2019, 'Incidence of appropriate implantable cardioverter-defibrillator therapy and mortality after implantable cardioverter-defibrillator generator replacement: results from a real-world nationwide cohort', EP Europace, vol. 21, no. 8, pp. 1211-1219. https://doi.org/10.1093/europace/euz121

Olsen, T, Jørgensen, OD, Nielsen, JC, Thøgersen, AM, Philbert, BT & Johansen, JB 2019, 'Incidence of device-related infection in 97 750 patients: clinical data from the complete Danish device-cohort (1982-2018)', European Heart Journal, vol. 40, no. 23, pp. 1862-1869. https://doi.org/10.1093/eurheartj/ehz316

Ibáñez, L, Sabaté, M, Vidal, X, Ballarin, E, Rottenkolber, M, Schmiedl, S, Heeke, A, Huerta, C, Martin Merino, E, Montero, D, Leon-Muñoz, LM, Gasse, C, Moore, N, Droz, C, Lassalle, R, Aakjaer, M, Andersen, M, De Bruin, ML, Groenwold, R, van den Ham, R, Souverein, P, Klungel, O & Gardarsdottir, H 2019, 'Incidence of direct oral anticoagulant use in patients with nonvalvular atrial fibrillation and characteristics of users in 6 European countries (2008–2015): A cross-national drug utilization study', British Journal of Clinical Pharmacology, vol. 85, no. 11, pp. 2524-2539. https://doi.org/10.1111/bcp.14071

Musliner, KL , Liu, X , Gasse, C , Christensen, KS , Wimberley, T & Munk-Olsen, T 2019, 'Incidence of medically treated depression in Denmark among individuals 15-44 years old: A comprehensive overview based on population registers', Acta Psychiatrica Scandinavica, vol. 139, no. 6, pp. 548-557. https://doi.org/10.1111/acps.13028

Christiansen, MK, Haugaa, K, Svensson, A, Gilljam, T, Madsen, T, Hansen, J, Holst, AG, Bundgaard, H, Edvardsen, T, Svendsen, JH, Platonov, PG & Jensen, HK 2019, 'Incidence, predictors, and success of ventricular tachycardia catheter ablation in arrhythmogenic right ventricular cardiomyopathy (ARVC): A long-term cohort study from the Nordic ARVC registry', European Society of Cardiology Congress 2019, Paris, France, 31/08/2019 - 04/09/2019.

Soholm, J, Holm, DK, Mossner, B, Madsen, LW, Hansen, JF, Weis, N, Sauer, AP, Awad, T & Christensen, PB 2019, 'Incidence, prevalence and risk factors for hepatitis C in Danish prisons', PLoS One, vol. 14, no. 7, 0220297. https://doi.org/10.1371/journal.pone.0220297

Reinke, R, Mathiesen, JS, Larsen, SR, Hahn, CH, Pedersen, HB, Bentzen, J, Schytte, S, Godballe, C, Londero, SC & A study from The Danish Thyroid Cancer Group – DATHYRCA (part of the DAHANCA organization) 2019, 'Incidental and Non-incidental Papillary Thyroid Microcarcinoma in Denmark 1996–2015: A national study on incidence, outcome and thoughts on active surveillance', Cancer epidemiology, vol. 60, pp. 46-50. https://doi.org/10.1016/j.canep.2019.03.011

Bengtsson, C, Jonsson, L, Holmström, M, Hellgren, J, Franklin, K, Gíslason, T, Holm, M, Johannessen, A, Jõgi, R, Schlünssen, V, Janson, C & Lindberg, E 2019, 'Incident Chronic Rhinosinusitis Is Associated With Impaired Sleep Quality: Results of the RHINE Study', The Journal of Clinical Sleep Medicine, vol. 15, no. 6, pp. 899-905. https://doi.org/10.5664/jcsm.7846

Jensen, AK, Uggeldahl, KC, Jacobsen, BH, Jensen, JD & Hasler, B 2019, 'Including aesthetic and recreational values in cost-effectiveness analyses of land use change based nitrogen abatement measures in Denmark', Journal of Environmental Management, vol. 240, pp. 384–393. https://doi.org/10.1016/j.jenvman.2019.03.076

Poier, PP & Jensen, F 2019, 'Including implicit solvation in the bond capacity polarization model', Journal of Chemical Physics, vol. 151, no. 11, 114118. https://doi.org/10.1063/1.5120873

Ehlers, B & Bilde, T 2019, 'Inclusive fitness, asymmetric competition and kin selection in plants', Oikos, vol. 128, no. 6, pp. 765-774. https://doi.org/10.1111/oik.06390

Pedersen, PJ & Caner, A 2019 'Income distribution among Turkish immigrants in Germany and Denmark' The Rockwool Foundation Research Unit, København.

Sovacool, BK , Kester, J , Noel, L & de Rubens, GZ 2019, 'Income, political affiliation, urbanism and geography in stated preferences for electric vehicles (EVs) and vehicle-to-grid (V2G) technologies in Northern Europe', Journal of Transport Geography, vol. 78, pp. 214-229. https://doi.org/10.1016/j.jtrangeo.2019.06.006

Wu, S & Wu, H 2019, 'Incorporating Biochar into Wastewater Eco-treatment Systems: Popularity, Reality, and Complexity', Environmental Science & Technology, vol. 53, no. 7, pp. 3345-3346. https://doi.org/10.1021/acs.est.9b01101

Nardello, M, Han, S, Møller, C & Gøtze, J 2019, 'Incorporating process and data heterogeneity in enterprise architecture: Extended AMA4EA in an international manufacturing company', Computers in Industry, vol. 115, 103178. https://doi.org/10.1016/j.compind.2019.103178

Jahangiri, A , Møller, AH, Madsen, B, Jørnsgaard, B, Vaerbak, S , Danielsen, M & Dalsgaard, TK 2019, 'Incorporation of bixin in aqueous media: Self-formulation with sorbitol ester of norbixin', Food Chemistry, vol. 294, pp. 433-439. https://doi.org/10.1016/j.foodchem.2019.05.075

Ozgur, K, Bulut, H, Berkkanoglu, M, Humaidan, P & Coetzee, K 2019, 'Increased body mass index associated with increased preterm delivery in frozen embryo transfers', Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology, vol. 39, no. 3, pp. 377-383. https://doi.org/10.1080/01443615.2018.1523883

Jensen, RE , Enkegaard, A & Steenberg, T 2019, 'Increased fecundity of Aphis fabae on Vicia faba plants following seed or leaf inoculation with the entomopathogenic fungus Beauveria bassiana', PLoS One, vol. 14, no. 10, e0223616. https://doi.org/10.1371/journal.pone.0223616

Bruun, TS, Højgaard, CK & Strathe, AV 2019, 'Increased feeding level in peak lactation is an effective tool to control body weight loss in hyper prolific sows', Journal of Animal Science, vol. 97, no. Suppl. 2, 184. https://doi.org/10.1093/jas/skz122.191

Mikkelsen, LF, Nordestgaard, BG, Schnohr, P & Ellervik, C 2019, 'Increased Ferritin Concentration and Risk of Atrial Fibrillation and Heart Failure in Men and Women: Three Studies of the Danish General Population Including 35799 Individuals', Clinical Chemistry, vol. 65, no. 1, pp. 180-188. https://doi.org/10.1373/clinchem.2018.292763

Metaxas, A, Thygesen, C, Briting, SRR, Landau, A, Darvesh, S & Finsen, B 2019, 'Increased Inflammation and Unchanged Density of Synaptic Vesicle Glycoprotein 2A (SV2A) in the Postmortem Frontal Cortex of Alzheimer’s Disease Patients', Frontiers in Cellular Neuroscience, vol. 13, 538. https://doi.org/10.3389/fncel.2019.00538

Alexander Kleber, B 2019, 'Increased insula connectivity with speech motor regions in trained singers during resting-state', Organization for Human Brain Mapping (OHBM) Annual Meeting 2019, Rome, Italy, 09/06/2019 - 13/06/2019.

De Fønss, S , Ovesen, PG & Fuglsang, J 2019, 'Increased Insulin Requirements in Twin Pregnancy in Type 1 Diabetes', Diabetes Care, vol. 42, no. 10, pp. e164-e165. https://doi.org/10.2337/dc19-0859

Kampmann, FB, Thuesen, ACB, Hjort, L, Bjerregaard, AA, Chavarro, J, Frystyk, J , Bjerre, M, Tetens, I, Olsen, S, Vaag, A, Damm, P & Grunnet, LG 2019, 'Increased leptin, decreased adiponectin and FGF21 concentrations in adolescent offspring of women with gestational diabetes', European Journal of Endocrinology, vol. 181, no. 6, pp. 691-700. https://doi.org/10.1530/EJE-19-0658

Hove, MJ, Vuust, P & Stupacher, J 2019, 'Increased levels of bass in popular music recordings 1955-2016 and their relation to loudness', The Journal of the Acoustical Society of America, vol. 145, no. 4, pp. 2247-2253. https://doi.org/10.1121/1.5097587

Hussain, AA, Hübel, C, Hindborg, M, Lindkvist, E, Kastrup, AM, Yilmaz, Z, Støving, RK, Bulik, CM & Sjögren, JM 2019, 'Increased lipid and lipoprotein concentrations in anorexia nervosa: A systematic review and meta-analysis', International Journal of Eating Disorders, vol. 52, no. 6, pp. 611-629. https://doi.org/10.1002/eat.23051

Alstrup, M , Mohanakumar, S & Hjortdal, VE 2019, 'Increased lymphatic contraction frequency in Fontan: Innate or adaptive response? A NIRF imaging study', 22nd Annual Update on Pediatric and Congenital Cardiovascular Disease, United States, 13/02/2019 - 17/02/2019 pp. NP4.

Alstrup, M , Mohanakumar, S & Hjortdal, VE 2019, 'Increased lymphatic contraction frequency in Fontan: Innate or adaptive response? A NIRF imaging study', World Journal for Pediatric and Congenital Heart Surgery, vol. 10, no. 2, pp. NP4.

Kyvsgaard, N , Mikkelsen, TS , Thastum, M, Christensen, AE, Wehner, PS, Nysom, K & Herlin, T 2019, 'Increased methotrexate intolerance in juvenile idiopathic arthritis compared to acute lymphoblastic leukaemia in children', PLoS One, vol. 14, no. 7, e0219539. https://doi.org/10.1371/journal.pone.0219539

Kugathasan, P, Stubbs, B, Aagaard, J, Jensen, SE, Munk Laursen, T & Nielsen, RE 2019, 'Increased mortality from somatic multimorbidity in patients with schizophrenia: a Danish nationwide cohort study', Acta Psychiatrica Scandinavica, vol. 140, no. 4, pp. 340-348. https://doi.org/10.1111/acps.13076

Rydbirk, R, Elfving, B, Folke, J, Pakkenberg, B, Winge, K, Brudek, T & Aznar, S 2019, 'Increased prefrontal cortex interleukin-2 protein levels and shift in the peripheral T cell population in progressive supranuclear palsy patients', Scientific Reports, vol. 9, no. 1, 7781. https://doi.org/10.1038/s41598-019-44234-y

Bugge, A, de Thurah, A , Andersen, IT , Hammerich Riis, A , Hauge, EM & Bøttcher, M 2019, 'Increased prevalence and severity of coronary artery disease in patients with chest pain and sero-positive rheumatoid arthritis: an analysis from a large scale cohort referred for cardiac computed tomography', EULAR, Madrid, Spain, 12/06/2019 - 15/06/2019.

Weisbjerg, MR , Guldberg, M , Hvelplund, T , Lund, P & Madsen, J 2019, 'Increased Productivity in Tanzanian Cattle is the Main Approach to Reduce Methane Emission per Unit of Product', Tanzanian Journal of Agricultural Sciences, vol. 18, no. 1, pp. 13-21. <https://www.ajol.info/index.php/tjags/article/view/192517/181624>

Klös, G , Miola, M & Sutherland, DS 2019, 'Increased Refractive Index Sensitivity by Circular Dichroism Sensing through Reduced Substrate Effect', Journal of Physical Chemistry C, vol. 123, no. 12, pp. 7347-7355. https://doi.org/10.1021/acs.jpcc.8b12152

Hagensen, MK , Mortensen, MB , Kjolby, M , Palmfeldt, J , Bentzon, JF & Gregersen, S 2019, 'Increased retention of LDL from type 1 diabetic patients in atherosclerosis-prone areas of the murine arterial wall', Atherosclerosis, vol. 286, pp. 156-162. https://doi.org/10.1016/j.atherosclerosis.2019.02.027

Kjørholt, KE , Kristensen, NR, Prieto-Alhambra, D, Johnsen, SP & Pedersen, AB 2019, 'Increased risk of mortality after postoperative infection in hip fracture patients', Bone, vol. 127, no. October, pp. 563-570. https://doi.org/10.1016/j.bone.2019.07.023

Ludmir, EB, Mahajan, A, Paulino, AC, Jones, JY, Ketonen, LM, Su, JM, Grosshans, DR, McAleer, MF, McGovern, SL, Lassen-Ramshad, YA, Adesina, AM, Dauser, RC, Weinberg, JS & Chintagumpala, MM 2019, 'Increased risk of pseudoprogression among pediatric low-grade glioma patients treated with proton versus photon radiotherapy', Neuro-Oncology, vol. 21, no. 5, pp. 686-695. https://doi.org/10.1093/neuonc/noz042

Wang, Y, Chen, J, Wang, Q, Qin, Q, Ye, J, Han, Y, Li, L, Zhen, W, Zhi, Q, Zhang, Y & Cao, J 2019, 'Increased secondary aerosol contribution and possible processing on polluted winter days in China', Environment International, vol. 127, pp. 78-84. https://doi.org/10.1016/j.envint.2019.03.021

Piper, TB, Jørgensen, LN, Olsen, J, Nielsen, KT, Davis, G, Johansen, JS, Jarle Christensen, I & Nielsen, HJ 2019, 'Increased serological, cancer-associated protein biomarker levels at diagnosis of large bowel adenoma: Risk of subsequent primary malignancy?', Acta Oncologica, vol. 58, no. sup1, pp. S42-S48. https://doi.org/10.1080/0284186X.2018.1540885

Ferneborg, S, Thulin, M, Agenäs, S, Svennersten-Sjaunja, K, Krawczel, P & Ternman, E 2019, 'Increased take-off level in automatic milking systems - Effects on milk flow, milk yield and milking efficiency at the quarter level', Journal of Dairy Research, vol. 86, no. 1, pp. 85-87. https://doi.org/10.1017/S002202991800078X

Displaying results 99251 to 99300 out of 389758

1986

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen