Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Chahine, A 2019, 'Looking for Mr. Right Now', Anthropology News, vol. 60, no. 1. https://doi.org/10.1111/AN.1071

Ramirez Gomez, A & Gellersen, H 2019, Looking Outside the Box: Reflecting on Gaze Interaction in Gameplay. in CHI PLAY 2019 - Proceedings of the Annual Symposium on Computer-Human Interaction in Play. pp. 625-637. https://doi.org/10.1145/3311350.3347150

Klausen, KC, Pagels, KØ, Bjerre, J (ed.) & Pedersen, CF (ed.) 2019, LoRa and LoRaWAN Case Studies. European Regional Development Fund and Central Denmark Region, Aarhus.

Knudsen, MB , Kæseler, SA & Rohde, J 2019, LoRaWAN End-Device Antenna Performance. in CEMi 2018 - International Workshop on Computing, Electromagnetics, and Machine Intelligence., 8610536, IEEE, pp. 77-78, IEEE CEMi'18, Stellenbosch, South Africa, 21/11/2018. https://doi.org/10.1109/CEMI.2018.8610536

Voigt, SW, Klemmensen, R & Serritzlew, S 2019, 'Losers Lose more than Winners Win: Asymmetrical Effects of Winning and Losing in Elections', European Journal of Political Research, vol. 58, no. 4, pp. 1172-1190. https://doi.org/10.1111/1475-6765.12329

Thinggaard, F & Schøler, F 2019, 'Løsning af regnskabsmæssige problemstillinger under ÅRL (årsregnskabsloven)', Revision & Regnskabsvaesen, vol. 2019, no. 10, pp. 20-31. <https://pro.karnovgroup.dk/document/7000842984/1>

Vestergaard, R , Zhang, Q & Lucani Rötter, DE 2019, Lossless Compression of Time Series Data with Generalized Deduplication. in 2019 IEEE Global Communications Conference, GLOBECOM 2019 - Proceedings., 9013957, IEEE, 2019 IEEE Global Communications Conference, Waikoloa, Hawaii, United States, 09/12/2019. https://doi.org/10.1109/GLOBECOM38437.2019.9013957

Sørensen, MP, Young, M & Pedersen, PB 2019, Lost in transition? on the migration to English language research publications. in MP Sørensen, L Geschwind, J Kekäle & R Pinheiro (eds), The responsible university: exploring the Nordic context and beyond. Palgrave Macmillan, Cham, pp. 87-114. https://doi.org/10.1007/978-3-030-25646-3_4

Thiergart, T, Zgadzaj, R, Bozsóki, Z, Garrido-Oter, R, Radutoiu, S & Schulze-Lefert, P 2019, 'Lotus japonicus Symbiosis Genes Impact Microbial Interactions between Symbionts and Multikingdom Commensal Communities', mBio, vol. 10, no. 5, e01833-19. https://doi.org/10.1128/mBio.01833-19

Helle, S 2019, 'Love isn’t what it was: How Disney took to subverting its own romantic ideals', Aeon. <https://aeon.co/essays/how-disney-took-to-subverting-its-own-romantic-ideals>

Kenter, JO, Raymond, CM, van Riper, CJ, Azzopardi, E, Brear, MR, Calcagni, F, Christie, I, Christie, M, Fordham, A, Gould, RK, Ives, CD, Hejnowicz, AP, Gunton, R, Horcea-Milcu, A-I, Kendal, D, Kronenberg, J, Massenberg, JR, O'Connor, S, Ravenscroft, N, Rawluk, A, Raymond, IJ, Rodríguez-Morales, J & Thankappan, S 2019, 'Loving the mess: navigating diversity and conflict in social values for sustainability', Sustainability science, vol. 14, no. 5, pp. 1439-1461.

Karstoft, S 2019, Lov om forsikringsformidling. in Karnovs lovsamling. 2019 edn, Karnov Group. <https://pro.karnovgroup.dk/document/7000840170/1>

Fanourakis, D, Gebraegziabher, HG , Hyldgaard, BN, Bouranis, D, Körner, O & Ottosen, C-O 2019, 'Low air humidity during growth promotes stomatal closure ability in rose', European Journal of Horticultural Science, vol. 84, no. 4, pp. 245-252. https://doi.org/10.17660/eJHS.2019/84.4.7

Mallonn, M, Köhler, J, Alexoudi, X, Von Essen, C, Granzer, T, Poppenhaeger, K & Strassmeier, KG 2019, 'Low albedos of hot to ultra-hot Jupiters in the optical to near-infrared transition regime', Astronomy and Astrophysics, vol. 624, A62. https://doi.org/10.1051/0004-6361/201935079

Nielsen, NH, Kristiansen, SM , Ljungberg, T , Enevold, R & Løvschal, M 2019, 'Low and variable: Manuring intensity in Danish Celtic fields', Journal of Archaeological Science: Reports, vol. 27, 101955. https://doi.org/10.1016/j.jasrep.2019.101955

Pieniowski, EHA, Palmer, GJ, Juul, T, Lagergren, P, Johar, A, Emmertsen, KJ, Nordenvall, C & Abraham-Nordling, M 2019, 'Low Anterior Resection Syndrome and Quality of Life After Sphincter-Sparing Rectal Cancer Surgery: A Long-term Longitudinal Follow-up', Diseases of the Colon & Rectum, vol. 62, no. 1, pp. 14-20. https://doi.org/10.1097/DCR.0000000000001228, https://doi.org/10.1097/DCR.0000000000001228

Tolborg, K 2019, 'Low barrier hydrogen bonds in negative thermal expansion material H3Co(CN)6', Danscatt 2019, Aarhus, Denmark, 23/05/2019 - 24/05/2019.

Tolborg, K , Jørgensen, MRV , Sist, M , Mamakhel, MAH , Overgaard, J & Iversen, BB 2019, 'Low‐Barrier Hydrogen Bonds in Negative Thermal Expansion Material H₃[Co(CN)₆]', Chemistry: A European Journal, vol. 25, no. 27, pp. 6814-6822. https://doi.org/10.1002/chem.201900358

Madsen, MN , Kirkegaard, ML , Laursen, M , Larsen, JR , Pedersen, MF , Skovgaard, B , PrynØ, T & Mikkelsen, LR 2019, 'Low complication rate after same-day total hip arthroplasty: a retrospective, single-center cohort study in 116 procedures', Acta Orthopaedica, vol. 90, no. 5, pp. 439-444. https://doi.org/10.1080/17453674.2019.1637631

Bøge Frederickson, L, Petersen-Sonn, EA, Shen, Y, Hertel, O, Hong, Y, Schmidt, J & Johnson, MS 2019, Low-cost sensors for indoor and outdoor pollution. in RA Meyers (ed.), Encyclopedia of sustainability science and technology. Springer, New York. https://doi.org/10.1007/978-1-4939-2493-6_1084-1

Tabori-Jensen, S , Hansen, TB & Stilling, M 2019, 'Low dislocation rate of Saturne®/Avantage^® dual-mobility THA after displaced femoral neck fracture: a cohort study of 966 hips with a minimum 1.6-year follow-up', Archives of Orthopaedic and Trauma Surgery, vol. 139, no. 5, pp. 605-612. https://doi.org/10.1007/s00402-018-3093-8

Bejder, L, Videsen, S , Hermannsen, L, Simon, M, Hanf, D & Madsen, PT 2019, 'Low energy expenditure and resting behaviour of humpback whale mother-calf pairs highlights conservation importance of sheltered breeding areas', Scientific Reports, vol. 9, no. 1, 771. https://doi.org/10.1038/s41598-018-36870-7

Farhadi, A, Hajiaghayi, MT, Larsen, KG & Shi, E 2019, Lower bounds for external memory integer sorting via network coding. in M Charikar & E Cohen (eds), STOC 2019 - Proceedings of the 51st Annual ACM SIGACT Symposium on Theory of Computing. Association for Computing Machinery, A C M Symposium on the Theory of Computing. Annual Proceedings, pp. 997-1008, 51st Annual ACM SIGACT Symposium on Theory of Computing, STOC 2019, Phoenix, United States, 23/06/2019. https://doi.org/10.1145/3313276.3316337

Afshani, P , Freksen, CB , Kamma, L & Larsen, KG 2019, 'Lower bounds for multiplication via network coding', Leibniz International Proceedings in Informatics, LIPIcs, vol. 132, 10. https://doi.org/10.4230/LIPIcs.ICALP.2019.10

Jacob, R, Larsen, KG & Nielsen, JB 2019, Lower Bounds for Oblivious Data Structures. in TM Chan (ed.), Proceedings of the Thirtieth Annual ACM-SIAM Symposium on Discrete Algorithms. Society for Industrial and Applied Mathematics, pp. 2439-2447, Thirtieth Annual ACM-SIAM Symposium on Discrete Algorithms, San Diego, California, United States, 06/01/2019. https://doi.org/10.1137/1.9781611975482.149

Aadal, L , Mortensen, J , Kellenberger, S & Nielsen, JF 2019, 'Lower Bowel Dysfunction Following Acquired Brain Injury: A Challenge During Rehabilitation', Gastroenterology Nursing, vol. 42, no. 1, pp. 12-19. https://doi.org/10.1097/SGA.0000000000000352

Vasseur, R, Boivin, S, Lathuilière, B, Lazar, I, Durlet, C, Martindale, RC, Bodin, S & Elhmidi, K 2019, 'Lower Jurassic corals from Morocco with skeletal structures convergent with those of Paleozoic rugosan corals', Palaeontologia Electronica, vol. 22, no. 2, 22.2.48A. https://doi.org/10.26879/874

Thomsen, PT , Foldager, L , Raundal, P & Capion, N 2019, 'Lower odds of sole ulcers in the following lactation in dairy cows that received hoof trimming around drying off', Veterinary Journal, vol. 254, 105408. https://doi.org/10.1016/j.tvjl.2019.105408

Nielsen, MS 2019, Low frequency estimation of Lévy-driven moving averages. in ITISE 2019 International Conference on Time Series and Forecasting: Proceedings of Papers. pp. 104-114, International Conference on Time Series and Forecasting 2019, Granada, Spain, 25/09/2019. <https://itise.ugr.es/ITISE2019_Vol1.pdf>

Dinh, KM , Kaspersen, KA , Mikkelsen, S, Pedersen, OB, Petersen, MS, Thørner, LW, Hjalgrim, H, Rostgaard, K, Ullum, H & Erikstrup, C 2019, 'Low-grade inflammation is negatively associated with physical Health-Related Quality of Life in healthy individuals: Results from The Danish Blood Donor Study (DBDS)', PLoS One, vol. 14, no. 3, e0214468. https://doi.org/10.1371/journal.pone.0214468

Gyawali, B, Mishra, SR, Virani, SS & Kallestrup, P 2019, 'Low levels of ideal cardiovascular health in a semi-urban population of Western Nepal: A population-based, cross-sectional study', Heart Asia, vol. 11, no. 1, e011131. https://doi.org/10.1136/heartasia-2018-011131

Benmachiche, A, Benbouhedja, S, Zoghmar, A & Humaidan, P 2019, 'Low LH Level on the Day of GnRH Agonist Trigger Is Associated With Reduced Ongoing Pregnancy and Live Birth Rates and Increased Early Miscarriage Rates Following IVF/ICSI Treatment and Fresh Embryo Transfer', Frontiers in Endocrinology, vol. 10, 639, pp. 639. https://doi.org/10.3389/fendo.2019.00639

Jørgensen, SL & Bohn, MB 2019, 'Low-load blood flow restricted resistance strength training in patients suffering from various lower limb injuries: results from a pilot project', Region Midt Sports Seminar, Horsens, Denmark, 26/09/2019 pp. xx.

Tohidi, M , Kargaard Madsen, J & Moradi, F 2019, 'Low-Power High-Input-Impedance EEG Signal Acquisition SoC with Fully Integrated IA and Signal-Specific ADC for Wearable Applications', IEEE Transactions on Biomedical Circuits and Systems, vol. 13, no. 6, 2936534, pp. 1437-1450. https://doi.org/10.1109/TBCAS.2019.2936534

Danstrup, CS & Klug, TE 2019, 'Low rate of co-infection in complicated infectious mononucleosis', Danish Medical Journal, vol. 66, no. 9, A5564.

Shreya, S 2019, 'Low Restoration-Energy Differential Spin Hall Effect MRAM for High-Speed Nonvolatile SRAM Application', 20th International Symposium on Quality Electronic Design (, California, United States, 06/03/2019 - 07/03/2019. https://doi.org/10.1109/ISQED.2019.8697426

Saheem, W, Brewster, C & Lauring, J 2019, 'Low status expatriates', Journal of Global Mobility, vol. 7, no. 4, pp. 321-324. https://doi.org/10.1108/JGM-12-2019-074

Hathwar, VR, Nakamura, A, Kasai, H, Suekuni, K, Tanaka, HI, Takabatake, T, Iversen, BB & Nishibori, E 2019, 'Low-Temperature Structural Phase Transitions in Thermoelectric Tetrahedrite, Cu12Sb4S13, and Tennantite, Cu12As4S13', Crystal Growth & Design, vol. 19, no. 7, pp. 3979-3988. https://doi.org/10.1021/acs.cgd.9b00385

Halkjær, S , Iversen, J , Kyhl, L , Chevallier, J , Andreatta, F, Yu, F, Stoot, A, Camilli, L, Bøggild, P, Hornekær, L & Cassidy, AM 2019, 'Low-temperature synthesis of a graphene-based, corrosion-inhibiting coating on an industrial grade alloy', Corrosion Science, vol. 152, pp. 1-9. https://doi.org/10.1016/j.corsci.2019.01.029

Lyngsø, J , Ramlau-Hansen, CH , Bay, B , Ingerslev, HJ , Strandberg-Larsen, K & Kesmodel, US 2019, 'Low-to-moderate alcohol consumption and success in fertility treatment: a Danish cohort study', Human Reproduction, vol. 34, no. 7, pp. 1334-1344. https://doi.org/10.1093/humrep/dez050

van der Meer, QHA, Scott, JM, Serre, SH, Whitehouse, MJ, Kristoffersen, M, Le Roux, PJ & Pope, EC 2019, 'Low-δ ¹⁸ O zircon xenocrysts in alkaline basalts; a window into the complex carbonatite-metasomatic history of the Zealandia lithospheric mantle', Geochimica et Cosmochimica Acta, vol. 254, pp. 21-39. https://doi.org/10.1016/j.gca.2019.03.029

Hasman, H, Clausen, PTLC, Kaya, H, Hansen, F, Knudsen, JD, Wang, M, Holzknecht, BJ, Samulioniené, J, Røder, BL, Frimodt-Møller, N, Lund, O & Hammerum, AM 2019, 'LRE-Finder, a Web tool for detection of the 23S rRNA mutations and the optrA, cfr, cfr(B) and poxtA genes encoding linezolid resistance in enterococci from whole-genome sequences', Journal of Antimicrobial Chemotherapy, vol. 74, no. 6, pp. 1473-1476. https://doi.org/10.1093/jac/dkz092

Nielsen, KH 2019, 'Lsd', Weekendavisen, vol. Sektion 4 (Ideer), pp. 10.

Ottsen, CL 2019, 'Lucky to reach the top? Gendered perspectives on leadership acquisition across Qatar and Denmark ', Gender in Management: An International Journal, vol. 34, no. 7, pp. 541-553. https://doi.org/10.1108/GM-11-2017-0158

Hertel, O , Ellermann, T , Løfstrøm, P , Jensen, SS , Ketzel, M , Brandt, J , Geels, C , Christensen, JH , Frohn, LM , Nøjgaard, JK , Nielsen, IE , Winther, M , Plejdrup, MS , Nielsen, O-K & Poulsen, MB 2019, 'Luftforurening - forskning for forebyggelse', Miljø og sundhed, vol. 25, no. 2, pp. 28-30. <http://miljoogsundhed.sst.dk/blad/ms1902.pdf>

Jensen, SS , Winther, M & Løfstrøm, P 2019, 'Luftforurening fra krydstogtskibe i Københavns Havn og Aarhus havn', Trafikdage på Aalborg Universitet 2019, Aalborg, Denmark, 26/08/2019 - 27/08/2019.

Ellermann, T 2019, 'Luftforureningsniveauer i Danmark', Sundhedsstyrelsens Rådgivende Videnskabelige Udvalg for Miljø og Sundhed, Copenhagen, Denmark, 12/06/2019.

Alstrup, AKO & Wang, T 2019, 'Lugte- og smagssanserne: Komparativ fysiologi hos hvirveldyrene', Kaskelot, vol. 226, pp. 8-11.

Strøm, J , Høybye, MT , Laursen, M , Jørgensen, LB & Nielsen, CV 2019, 'Lumbar Spine Fusion Patients' Use of an Internet Support Group: Mixed Methods Study', Journal of Medical Internet Research, vol. 21, no. 7, 9805. https://doi.org/10.2196/jmir.9805

Constantin, D, Veres, D, Panaiotu, C, Anechitei-Deacu, V, Groza, SM, Begy, R, Kelemen, S, Buylaert, JP, Hambach, U, Marković, SB, Gerasimenko, N & Timar-Gabor, A 2019, 'Luminescence age constraints on the Pleistocene-Holocene transition recorded in loess sequences across SE Europe', Quaternary Geochronology, vol. 49, no. February, pp. 71-77. https://doi.org/10.1016/j.quageo.2018.07.011

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