Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Illeris, K 2003, From vocational training to workplace learning.

Naghavi, M, Libby, P, Falk, E, Casscells, SW, Litovsky, S, Rumberger, J, nullJ.J., B, Stefanadis, C, Moreno, P, Pasterkamp, G, Fayad, Z, Stone, PH, Waxman, S, Raggi, P, Madjid, M, Zarrabi, A, Burke, A, Yuan, C, Fitzgerald, PJ, Siscovick, DS, de Korte, CL, Aikawa, M, Juhani Airaksinen, KE, Assmann, G, Becker, CR, Chesebro, JH, Farb, A, Galis, ZS, Jackson, C, Jang, IK, Koenig, W, Lodder, RA, March, K, Demirovic, J, Navab, M, Priori, SG, Rekhter, MD, Bahr, R, Grundy, SM, Mehran, R, Colombo, A, Boerwinkle, E, nullC., B, Insull, WJ, Schwartz, RS, Vogel, R, Serruys, PW, Hansson, GK, Faxon, DP, nullS., K, Drexler, H, Greenland, P, Muller, JE, Virmani, R, Ridker, PM, Zipes, DP, Shah, PK & Willerson, JT 2003, 'From vulnerable plaque to vulnerable patient: a call for new definitions and risk assessment strategies: Part I', Circulation, vol. 108, no. 14, pp. 1664-1672.

Naghavi, M, Libby, P, Falk, E, Casscells, SW, Litovsky, S, Rumberger, J, Badimon, JJ, Stefanadis, C, Moreno, P, Pasterkamp, G, Fayad, Z, Stone, PH, Waxman, S, Raggi, P, Madjid, M, Zarrabi, A, Burke, A, Yuan, C, Fitzgerald, PJ, Siscovick, DS, de Korte, CL, Aikawa, M, Airaksinen, KE, Assmann, G, Becker, CR, Chesebro, JH, Farb, A, Galis, ZS, Jackson, C, Jang, IK, Koenig, W, Lodder, RA, March, K, Demirovic, J, Navab, M, Priori, SG, Rekhter, MD, Bahr, R, Grundy, SM, Mehran, R, Colombo, A, Boerwinkle, E, Ballantyne, C, Insull, WJ, Schwartz, RS, Vogel, R, Serruys, PW, Hansson, GK, Faxon, DP, Kaul, S, Drexler, H, Greenland, P, nullJ.E., M, Virmani, R, Ridker, PM, Zipes, DP, Shah, PK & Willerson, JT 2003, 'From vulnerable plaque to vulnerable patient: a call for new definitions and risk assessment strategies: Part II', Circulation, vol. 108, no. 15, pp. 1772-1778.

Boelt, B 2003, 'Frøvækst Øst: Midler til regional udvikling inden for frøavl', Froeavleren, vol. 2, pp. 24.

Olsen, AV, Tjønneland, A, Thomsen, B, Loft, S, Stripp, C, Overvad, K, Møller, S, Ewertz, MK & Olsen, JH 2003, 'Fruits and vegetables intake differentially affects estrogen receptor negative and positive breast cancer incidence rates', Journal of Nutrition, vol. 133, pp. 2342-7.

Hauge, H 2003, 'Frye og fremskridtet', Norsk litteraturvitenskapeligt tidsskrift, vol. 6, no. 1.

Malmkvist, J & Hansen, SW 2003, Frygt hos mink - årsager og betydning for velfærd. in Temamøde: Pelsdyrproduktion - adfærd, sundhed, avl og ernæring. pp. 5-15, Frygt hos mink - årsager og betydning for velfærd, 18/12/2010.

Clausen, P , Holm, TE & Ringkjøbing Amt (ed.) 2003, Fugle. in Ringkjøbing Amt (ed.), Miljøtilstanden i Thyborøn og Harboøre Fjord (lagunesøerne) på Harboøre Tange 1998-2001. Ringkjøbing Amt, pp. 118-121.

Iversen, T 2003, Fuldmagt. in M Koktvedgaard, J Møller & B Carlsen (eds), Juridisk Formularsamling. 10. elektroniske edn, Djøf Forlag, København. <http://www.djoef-forlag.dk/servlet/djof.showArticle?vnr=8733345007&ident=artShow>

Nygaard, M, Winskel, G, Amadio, R (ed.) & Lugiez, D (ed.) 2003, Full Abstraction for HOPLA. in G Goos, J van Leeuwen & J Hartmanis (eds), Lecture Notes in Computer Science: Concur 2003 - Concurrency Theory. vol. 2761. Mobility, Springer, pp. 383-398, Concur 2003 - Concurrency Theory, Marseille, France, 03/09/2003.

Hasselstrøm, J & Linnet, K 2003, 'Fully automated on-line quantification of quetiapine in human serum by solid phase extraction and liquid chromatography', J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., vol. 798, no. 1, pp. 9-16.

Matthiesen, TB, Rittig, S & Djurhuus, JC 2003, 'Functional bladder capacity and urodynamics in males with nocturia', Acta Pathologica Microbiologica et Immunologica Scandinavica, vol. suppl. 109, pp. 59-65.

Toustrup-Jensen, MS & Vilsen, B 2003, 'Functional consequences of alterations to Ile²⁷⁹, Ile²⁸³, Glu²⁸⁴, His²⁸⁵, Phe²⁸⁶, and His²⁸⁸, in the NH₂-terminal part of transmembrane helix M3 of theNa⁺,K⁺-ATPase', Journal of Biological Chemistry, vol. 278, pp. 38653-38664.

Einholm, AP , Vilsen, B & Andersen, JP 2003, 'Functional consequences of charge reversals of acidic residues in M1 of the Ca-ATPase', New York Academy of Sciences. Annals, vol. 986, pp. 310-311.

Christiansen, M , Stevnsner, TV, Modin, C, Martensen, PM, Brosh Jr., RM & Bohr, V 2003, 'Functional consequences of mutations in the conserved SF2 motifs and post-translational phosphorylation of the CSB protein.', Nucleic Acids Res. , vol. 31, no. 3, pp. 963-973.

Christiansen, M, Stevnsner, T , Modin, C , Martensen, PM, Brosh Jr., RM & Bohr, V 2003, 'Functional consequences of mutations in the conserved SF2 motifs and posttranslational phosphorylation of the CSB protein', Nucl. Acids Res., vol. 31, pp. 963-973.

Ager, MS, Biernacki, D , Danvy, O & Midtgaard, J 2003, 'Functional Correspondence between Evaluators and Abstract Machines', BRICS Report Series, no. RS-03-13.

Birkedal, R & Gesser, H 2003, Functional coupling between mitochondria and cytosolic ATPases in rainbow trout ventricle. in Comp. Biochem. Physiol.. pp. 18-18, Abstracts of the Annual Main Meeting of the Society for Experimental Biology, 31 March -4 April, Southampton, UK, 17/12/2010.

Karring, H, Björnsson, A, Thirup, S , Clark, BFC & Knudsen, CR 2003, 'Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts', FEBS journal, vol. 270, pp. 4294-4305.

Brinch Pedersen, H , Gregersen, PL , Dionisio, G & Holm, PB 2003, Functional genomics approach to study phosphate allocation in wheat and as a tool for risk assesment of transgenic wheat. in Abstract selected for oral presentation. pp. 6, Plant GEMs, York, United Kingdom, 02/09/2003.

Petersen, HH, Hilpert, J, Militz, D, Zandler, V, Jacobsen, C, Roebroek, AJ & Willnow, TE 2003, 'Functional interaction of megalin with the megalinbinding protein (MegBP), a novel tetratrico peptide repeat-containing adaptor molecule', J. Cell Sci., no. 116, pp. 453-461.

Andersen, JR & Lübberstedt, T 2003, 'Functional markers in plants', Trends in Plant Science, vol. 8, no. 11, pp. 554-560. https://doi.org/10.1016/j.tplants.2003.09.010

Cornelius, F & Mahmmoud, YA 2003, 'Functional modulation of the sodium-pump:The regulatory proteins "fix-it"', News Physiol. Sci., no. 18, pp. 119-124.

Tiselius, P, Petersen, JK , Nielsen, TG , Maar, M , Møller, EF, Satapoomin, S, Tönnesson, K, Zervoudaki, T, Christou, E, Giannakourou, A, Sell, A & Vargas, C 2003, 'Functional response of Oikopleura dioica to house clogging due to exposure to algae of different sizes', Marine Biology, vol. 142, pp. 253-261.

Althage, M, Karlsson, J, Gourdon, P, Levin, M, Bill, RM, Tigerström, A & Rydström, J 2003, 'Functional split and crosslinking of the membrane domain of the beta subunit of proton-translocating transhydrogenase from Escherichia coli', Biochemistry, vol. 42, no. 37, pp. 10998-1003. https://doi.org/10.1021/bi034560x

Sørensen, CB , Andresen, BS , Jensen, UB , Jensen, TG , Jensen, PKA , Gregersen, N & Bolund, L 2003, 'Functional testing of keratin 14 mutant proteins associated with the three major subtypes of epidermolysis bullosa simplex', Experimental Dermatology, vol. 12, no. 4, pp. 472-9.

Kronvang, B, Morawska, D (ed.), Siedlecki, T (ed.) & Stelmaszczyk, M (ed.) 2003, Functioning and importance of lowland river floodplains as sinks for sediment, organic matter and nutrients during flooding. in D Morawska, T Siedlecki & M Stelmaszczyk (eds), Proceedings of the Warzaw Conference of ECOFLOOD, Towards natural flood reduction strategies, 6-13 September 2003. Warsaw Agricultural University. <http://levis.sggw.waw.pl/ecoflood>

Pedersen, L 2003, Fundamental rheological measurements of wheat flour dough and gluten and relationship to baking quality. in Nye trender innenfor næringsmiddelanalyser, KVL, 13-15 oktobe. pp. 2003-2003, Fundamental rheological measurements of wheat flour dough and gluten and relationship to baking quality, 18/12/2010.

Togeby, O 2003, Fungerer denne sætning? Funktionel dansk sproglære. Gad, København.

Steenberg, T & Kilpinen, O 2003, Fungus infection of the chicken mite Dermnayssus gallinae. in IOBC/wprs Bulletin. 1 edn, vol. 26, pp. 23-26.

Fink, P 2003, 'Funktionelle lidelser: Historisk baggrund del 3', Månedskrift for Praktisk Lægegerning, vol. 82, no. 6, pp. 829-836.

Fink, P 2003, 'Funktionelle lidelser: Historisk baggrund del 2', Månedskrift for Praktisk Lægegerning, vol. 81, no. 5, pp. 679-689.

Fink, P 2003, 'Funktionelle lidelser: Historisk baggrund del 1', Månedskrift for Praktisk Lægegerning, vol. 81, no. 4, pp. 501-509.

Fink, P 2003, 'Funktionelle Lidelser', Medicus, vol. 2, pp. 10-11.

Holm, PB 2003, 'Fup eller fakta', Weekendavisen.

Warncke, E & Kwee, S 2003, 'Fup og fakta: Viden skal - modsat tro - kunne undersøges og eventuelt dømmes falsk. Naturvidenskab beskæftiger sig med viden, så det burde være overflødigt at diskutere elementær stofoptagelse og -omsætning i kroppen. Men sådan forholder det sig åbenbart ikke i alle dele af sundhedssektoren.', Fyns Stiftstidende.

Christiansen, CB, Madsen, PB & Christensen, M 2003 'Further Evidence on Hedge Funds Performance'.

Mikkelsen, LM, Jensen, CM, Høj, B, Blakskjær, P & Skrydstrup, T 2003, 'Further studies in the acyl-type radical additions promoted by SmI2: mechanistic implications and stereoselective reduction of the keto-functionality', Tetrahedron, vol. 59, pp. 10541-10549.

Yin, C, Rosendahl, LA & Condra, TJ 2003, 'Further study of the gas temperature deviation in large-scale tangentially coal-fired boilers', Fuel, vol. 82, no. 9, pp. 1127-1137. https://doi.org/10.1016/S0016-2361(02)00418-0

Vester, P 2003, 'Fyn - pioner på plantemedicinområdet', Nyhedsbrevet, Danmarks JordbrugsForskning, no. 4, pp. 4-5.

Bach-Nielsen, C & Ingeman, P (ed.) 2003, Fyrstebibel eller folkebibel: Reformationsfyrsternes personlige engagement i oversættelsen af Bibelen til folkesprogene. in Reformation: religion og politik. Aarhus Universitetsforlag, Århus, pp. 73-125.

Carlson, D & Poulsen, HD 2003, Fysiologiske effekter af zink og kobber til fravænnede grise. in Optimering af mave-tarmmiljøet hos grise omkring fravænning. (Eds. Jensen, B.B. & Pedersen, L.T.) DJF intern rapport 174. pp. 72-75, Fysiologiske effekter af zink og kobber til fravænnede grise, 18/12/2010.

Sejrsen, K 2003, Fysiologisk tilpasning til høj ydelse - Træk eller tryk? in Dansk Kvæg Kongres 2003. pp. 92-93, Fysiologisk tilpasning til høj ydelse - Træk eller tryk?, 18/12/2010.

Schriver, N 2003, 'Fysioterapi og læring: Betydning af rettethed, relationer, rum og refleksion', Århus.

Poulsen, HD & Blaabjerg, K 2003, 'Fytase i svinefoder - en fantastisk hjælper, som skal bruges med omtanke', Nyhedsbrevet, Danmarks JordbrugsForskning, vol. 4, pp. 1-2.

Jensen, K, Chiu, CS, Sokolova, I, Lester, HA & Mody, I 2003, 'GABA transporter-1 (GAT1)-deficient mice: differential tonic activation of GABA_A versus GABA_B receptors in the hippocampus', Journal of Neurophysiology, vol. 90, pp. 2690-2701.

Mørck, LL 2003, Gadeplansarbejderuddannelse som del af det københavnske socialarbejdermiljø. København. <http://www.vildelaereprocesser.dk/drift/uddannelse/uddannelsetekst.php?id=127>

Mørck, LL 2003, 'Gadeplansarbejder uddannelse som del af det Københavnske socialarbejdermiljø', www.vildelaereprocesser.dk.

Jensen, BE 2003, Gads Historieleksikon. 2. udv. udg. edn, Gad, København.

Displaying results 325901 to 325950 out of 389685

6519