Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Eyerich, K, Brown, SJ, Perez White, BE, Tanaka, RJ, Bissonette, R, Dhar, S, Bieber, T, Hijnen, DJ, Guttman-Yassky, E, Irvine, A, Thyssen, JP, Vestergaard, C, Werfel, T, Wollenberg, A, Paller, AS & Reynolds, NJ 2019, 'Human and computational models of atopic dermatitis: A review and perspectives by an expert panel of the International Eczema Council', The Journal of Allergy and Clinical Immunology, vol. 143, no. 1, pp. 36-45. https://doi.org/10.1016/j.jaci.2018.10.033

Frisch, K , Kjærgaard, K , Horsager, J , Schacht, AC & Munk, OL 2019, 'Human biodistribution, dosimetry, radiosynthesis and quality control of the bile acid PET tracer [N-methyl-11C]cholylsarcosine', Nuclear Medicine and Biology, vol. 72-73, no. May-June, pp. 55-61. https://doi.org/10.1016/j.nucmedbio.2019.07.006

Poulsen, KO, Meng, F, Lanfranchi, E, Kelly, A & Sundekilde, UK 2019, 'Human breast milk metabolomes over the course of 6 months lactation', 16th Symposium on Milk Genomics and Human Health, Aarhus, Denmark, 12/11/2019 - 14/11/2019.

Alavi, HS, Churchill, EF, Wiberg, M, Lalanne, D, Dalsgaard, P, gen Schieck, AF & Rogers, Y 2019, 'Human-building Interaction: Sketches and Grounds for a Research Program', interactions, vol. 26, no. 4, pp. 58-61. https://doi.org/10.1145/3330342

Frank, D-A , Chrysochou, P , Mitkidis, P & Ariely, D 2019, 'Human decision-making biases in the moral dilemmas of autonomous vehicles', Scientific Reports, vol. 9, no. 1, 13080. https://doi.org/10.1038/s41598-019-49411-7

Xu, F, Eulaers, I, Alves, A, Papadopoulou, E, Padilla-Sanchez, JA, Lai, FY, Haug, LS, Voorspoels, S, Neels, H & Covaci, A 2019, 'Human exposure pathways to organophosphate flame retardants: Associations between human biomonitoring and external exposure', Environment International, vol. 127, pp. 462-472. https://doi.org/10.1016/j.envint.2019.03.053

Sonne, C , Vorkamp, K , Galatius, A , Kyhn, LA , Teilmann, J , Bossi, R , Søndergaard, J , Eulaers, I , Desforges, J-P , Siebert, U & Dietz, R 2019, 'Human exposure to PFOS and mercury through meat from baltic harbour seals (Phoca vitulina)', Environmental Research, vol. 175, pp. 376-383. https://doi.org/10.1016/j.envres.2019.05.026

Gomez-Ospina, N, Scharenberg, SG, Mostrel, N, Bak, RO, Mantri, S, Quadros, RM, Gurumurthy, CB, Lee, C, Bao, G, Suarez, CJ, Khan, S, Sawamoto, K, Tomatsu, S, Raj, N, Attardi, LD, Aurelian, L & Porteus, MH 2019, 'Human genome-edited hematopoietic stem cells phenotypically correct Mucopolysaccharidosis type I', Nature Communications, vol. 10, no. 1, 4045, pp. 4045. https://doi.org/10.1038/s41467-019-11962-8

Dupont, MA, Humbert, C, Huber, C, Siour, Q, Guerrera, IC, Jung, V, Christensen, A, Pouliet, A, Garfa-Traore, M, Nitschké, P, Injeyan, M, Millar, K, Chitayat, D, Shannon, P, Girisha, KM, Shukla, A, Mechler, C, Lorentzen, E, Benmerah, A, Cormier-Daire, V, Jeanpierre, C, Saunier, S & Delous, M 2019, 'Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion', Human Molecular Genetics, vol. 28, no. 16, pp. 2720-2737. https://doi.org/10.1093/hmg/ddz091

Løgstrup, KE, van Kooten Niekerk, K (Translator), Rabjerg, B (Translator) & Stern, R (Translator) 2019, '"Humanism and Christianity": Translated by Kees van Kooten Niekerk, Bjørn Rabjerg and Robert Stern', Zeitschrift für Neuere Theologiegeschichte, vol. 26, no. 1, pp. 132-146. https://doi.org/10.1515/znth-2019-0006

Just, J , Munk Ipsen, P, Kruhøffer, M, Lykkemark, S , Skjold, T , Schiøtz, PO & Hoffmann, HJ 2019, 'Human Mast Cell Sensitization with IgE Increases miRNA-210 Expression', International Archives of Allergy and Immunology, vol. 179, no. 2, pp. 102-107. https://doi.org/10.1159/000496513

Poulsen, KO , Young, JF, Yang, F & Sundekilde, UK 2019, 'Human milk-omics: - a study of breast milk constituents on infant gut microbial colonization and metabolism early in life', Arla Food For Health Conference, Aarhus , Denmark, 02/05/2019 - 02/05/2019.

Petersen, E, Kantele, A, Koopmans, M, Asogun, D, Yinka-Ogunleye, A, Ihekweazu, C & Zumla, A 2019, 'Human monkeypox: epidemiologic and clinical characteristics, diagnosis, and prevention', Infectious Disease Clinics of North America, vol. 33, no. 4, pp. 1027-1043. https://doi.org/10.1016/j.idc.2019.03.001

Herce Castañón, S, Moran, R, Ding, J, Egner, T, Bang, D & Summerfield, C 2019, 'Human noise blindness drives suboptimal cognitive inference', Nature Communications, vol. 10, no. 1, 1719. https://doi.org/10.1038/s41467-019-09330-7

Miguel-Escalada, I, Bonas-Guarch, S, Cebola, I, Ponsa-Cobas, J, Mendieta-Esteban, J, Atla, G, Javierre, BM, Rolando, DMY, Farabella, I, Morgan, CC, Garcia-Hurtado, J, Beucher, A, Moran, I, Pasquali, L, Ramos-Rodriguez, M, Appel, EVR, Linneberg, A, Gjesing, AP, Witte, DR, Pedersen, O, Grarup, N, Ravassard, P, Torrents, D, Mercader, JM, Piemonti, L, Berney, T, de Koning, EJP, Kerr-Conte, J, Pattou, F, Fedko, IO, Groop, L, Prokopenko, I, Hansen, T, Marti-Renom, MA, Fraser, P & Ferrer, J 2019, 'Human pancreatic islet three-dimensional chromatin architecture provides insights into the genetics of type 2 diabetes', Nature Genetics, vol. 51, no. 7, pp. 1137-1148. https://doi.org/10.1038/s41588-019-0457-0

Music, S, Salvietti, G, Dohman, PBG, Chinello, F, Prattichizzo, D & Hirchee, S 2019, 'Human-Robot Team Interaction Through Wearable Haptics for Cooperative Manipulation', IEEE Transactions on Haptics, vol. 12, no. 3, 8733002, pp. 350-362. https://doi.org/10.1109/TOH.2019.2921565

Løvschal, M, Gullbekk, SH, Johansen, M-L , O'neill, S & Willerslev, R 2019, 'Human Sacrifice and Value', Antiquity, vol. 93, no. 370, e23. https://doi.org/10.15184/aqy.2019.104

Yu, Y, zhang, Z, Zhang, X & Zeng, W 2019, 'Human-scale Quality on Streets: A Large-scale and Efficient Analytical Approach Based on Street View Images and New Urban Analytical Tools', 国际城市规划 Urban Planning International, vol. 34, no. 1, pp. 18-27. https://doi.org/10.22217/upi.2018.490

Lafaille, FG, Harschnitz, O, Lee, YS, Zhang, P, Hasek, ML, Kerner, G, Itan, Y, Ewaleifoh, O, Rapaport, F, Carlile, TM, Carter-Timofte, ME, Paquet, D, Dobbs, K, Zimmer, B, Gao, D, Rojas-Duran, MF, Kwart, D, Rattina, V, Ciancanelli, MJ, McAlpine, JL, Lorenzo, L, Boucherit, S, Rozenberg, F, Halwani, R, Henry, B, Amenzoui, N, Alsum, Z, Marques, L, Church, JA, Al-Muhsen, S, Tardieu, M, Bousfiha, AA, Paludan, SR , Mogensen, TH, Quintana-Murci, L, Tessier-Lavigne, M, Smith, GA, Notarangelo, LD, Studer, L, Gilbert, W, Abel, L, Casanova, JL & Zhang, SY 2019, 'Human SNORA31 variations impair cortical neuron-intrinsic immunity to HSV-1 and underlie herpes simplex encephalitis', Nature Medicine, vol. 25, no. 12, pp. 1873-1884. https://doi.org/10.1038/s41591-019-0672-3

Guo, XX, Liu, HT & Wu, SB 2019, 'Humic substances developed during organic waste composting: Formation mechanisms, structural properties, and agronomic functions', Science of the Total Environment, vol. 662, pp. 501-510. https://doi.org/10.1016/j.scitotenv.2019.01.137

Høiris, O 2019, Hunden. in O Høiris & PKA Jensen (eds), Evolutionens menneske – menneskets evolution. Aarhus Universitetsforlag, Aarhus, pp. 305-320.

Watt, CA, Doniol-Valcroze, T, Witting, L, Hobbs, R, Hansen, RG, Lee, DS, Marcoux, M, Lesage, V, Garde, E, Ferguson, SH & Heide-Jørgensen, MP 2019, 'Hunt Allocation Modeling for Migrating Animals: The Case of Baffin Bay Narwhal, Monodon monoceros', Marine Fisheries Review, vol. 81, no. 3-4, pp. 125-136. https://doi.org/10.7755/MFR.81.3-4.8

Silvers, JA, Squeglia, LM, Rømer Thomsen, K, Hudson, KA & Feldstein Ewing, SW 2019, 'Hunting for What Works: Adolescents in Addiction Treatment', Alcoholism: Clinical and Experimental Research, vol. 43, no. 4, pp. 578-592. https://doi.org/10.1111/acer.13984

Kveiborg, J 2019, Husdyrhold og fiskeri i yngre jernalder: En zooarkæologisk analyse af knoglemateriale fra Bejsebakken. in TB Sarauw (ed.), Bejsebakken : En nordjysk bebyggelse fra yngre jernalder og vikingetid. Det Kongelige Nordiske Oldskriftselskab, København, pp. 199-215.

Poulsen, HD , Møller, HB , Klinglmair, M & Thomsen, M 2019, Husdyrs fosforudnyttelse og fosfors værdikæde fra husdyrgødning, bioaffald og spildevand: Faglig baggrundsrapport for fosforvidensyntese. Aarhus Universitet, DCE – Nationalt Center for Miljø og Energi, 82 s. - Videnskabelig rapport nr. 325. Videnskabelig rapport fra DCE - Nationalt Center for Miljø og Energi, no. 325, Aarhus University, DCE - Danish Centre for Environment and Energy. <http://dce2.au.dk/pub/SR325.pdf>

Dyrlund Wåhlin-Jacobsen, C, Andersen, MF & Dalgaard, VLP 2019, 'Husk arbejdet i den arbejdsrelaterede stress', Ugeskrift for Læger, vol. 181, no. 15, pp. 1450.

Schild, S-LA , Foldager, L, Bonde, MK, Andersen, HM-L & Pedersen, LJ 2019, Hut climate impacts piglet survival. in Book of Abstracts of the 70th Annual Meeting of the European Federation of Animal Science. vol. 25, Wageningen Academic Publishers, Wageningen, The Netherlands, pp. 338, 70th Annual Meeting of the European Federation of Animal Science EAAP 2019, Ghent, Belgium, Ghent, Belgium, 26/08/2019. https://doi.org/10.3920/978-90-8686-890-2

Tafdrup, J (ed.) & Grundtvig, NFS 2019, Hvad Dagen hør til. Grundtvigs Værker. <http://www.xn--grundtvigsvrker-7lb.dk/tekstvisning/19738/0#>

Damgaard-Møller, SP & Lomholt, JJ 2019, 'Hvad driver de ambitiøse elever? En kritisk analyse af brugbarheden af Dwecks teori som forklaringsmodel for mentale karakteristika hos ”12-tals piger”', Pædagogisk Psykologisk Tidsskrift, vol. 56, no. 5/6, pp. 102-113.

Tipsmark, KS 2019, Hvad er en herreborg?. danmarkshistorien.dk. <https://danmarkshistorien.dk/leksikon-og-kilder/vis/materiale/hvad-er-en-herreborg/>

Eriksson, B 2019, 'Hvad er en mor? Sansemættet italiensk roman om mødre, døtre og familier uden tryghed ', Litteraturmagasinet Standart, vol. 33, no. 2, pp. 13.

Andersen, S 2019, Hvad er etik og moral. Etik.dk. <https://www.etik.dk/temaer-etik.dk/hvad-er-etik-og-moral>

Skyggebjerg, AK 2019, 'Hvad er faglitteratur for børn? Anmeldelse af Joe Sutcliff Sanders: A Literature of Questions. Non-fiction for the Critical Child', Sakprosa , vol. 11, no. 3, pp. 1-5. https://doi.org/10.5617/sakprosa.6935

Rønkilde, JB 2019, 'Hvad er meningen? Nyere gudstjenesteteorier og deres betydning for gudstjenesteteologi i Danmark', Dansk Tidsskrift for Teologi og Kirke, vol. 02, no. 3, pp. 101-117. https://doi.org/10.1080/13691457.2017.1366427

Jørgensen, EK & Kjærulff, BM 2019, 'Hvad er middelalderisme? Redaktionelt forord', Slagmark: tidsskrift for idéhistorie , no. 79, pp. 7-15. https://doi.org/10.7146/slagmark.vi79.130725

Jepsen, CJ 2019, Hvad er samtidskunst? en kort begrebshistorie om samtidskunstens 'samtidighed'. in C Juel Jepsen, R Kjærboe, S Krogh & M Søberg (eds), Terræn: Veje ind i samtidskunsten. Aarhus Universitetsforlag, Aarhus, pp. 98-109.

Jensen, NR 2019, Hvad fremmer hhv. hæmmer skolepædagogers arbejde. in Hvad fremmer hhv. hæmmer. 1. udgave edn, DPU - Danmarks Institut for Pædagogik og Uddannelse, København, pp. 5-46.

Anneberg, I 2019, Hvad fremmer og hvad hindrer landmænd og dyrlæger i at reducere brugen af antibiotika og medicinsk zink til svin? vol. 147, DCA - Nationalt Center for Fødevarer og Jordbrug. <http://web.agrsci.dk/djfpublikation/index.asp?action=show&id=1290>

Andersen, FØ 2019, Hvad gør ghettoskolen, der kan sikre sine elever god uddannelse og vellønnede job? Vi kender alle ingredienserne, og selv om opskriften på p-tech gryderetten virker ny, har vi faktisk tilbudt den herhjemme i form af de såkaldte t-klasser tilbage i 1959-75. Folkeskolen.dk. <https://www.folkeskolen.dk/660115/hvad-goer-ghettoskolen-der-kan-sikre-sine-elever-god-uddannelse-og-velloennede-job>

Skewes, L 2019, 'Hvad gør vi i Danmark mod sexchikane - og hvad kan vi gøre bedre?', Women, Gender & Research, vol. 1-2, no. 19, pp. 118. https://doi.org/10.7146/kkf.v28i1-2.116137

Jensen, NR , Kjeldsen, CC & Bjerre, J 2019, Hvad hæmmer hhv. fremmer skolepædagogers samarbejde om stærke faglige fællesskaber for lavtpræsterende drenge i indskolingen? in NR Jensen (ed.), Hvad fremmer hhv. hæmmer ..... 1. udgave edn, DPU - Danmarks Institut for Pædagogik og Uddannelse, København , pp. 5-46.

Hansen, OH 2019, 'Hvad har vi gang i? Hvad tænker vi på?', 0-14 : nul til fjorten, vol. 2019, no. 1, pp. 70-74.

Høghøj, M 2019, Hvad kan vi lære af 'betonjunglens' historie? 1. del. Eftertrykket.dk.

Høghøj, M 2019, Hvad kan vi lære af 'betonjunglens' historie? 2. del. Eftertrykket.dk.

Ejrnæs, R & Nygaard, B 2019, 'Hvad skal der til for at opnå gunstig bevaringsstatus efter Habitatdirektivet og standse tab af biodiversitet i Danmark?', 14 p., Notat fra DCE - Nationalt Center for Miljø og Energi (2011-2019). <https://dce.au.dk/fileadmin/dce.au.dk/Udgivelser/Notater_2019/Gunstig_bevaringsstatus_efter_HD.pdf>

Aagaard, A 2019, 'Hvad skal der til for at sikre vækst i Trekantområdet? Det kan opsummeres i tre ord', Jyllands-Posten.

Baunvig, KF 2019, 'Hvad skal vi egentlig med Grundtvig?', Kristeligt Dagblad, vol. Sektion 1, pp. 10-10. <https://www.kristeligt-dagblad.dk/kommentar/hvad-skal-vi-egentlig-med-grundtvig>

Skiveren, T 2019, 'Hvad sker der i dansk samtidslyrik', Jyllands-Posten, vol. Bøger, pp. 26.

Grøn, CH & Salomonsen, HH 2019, 'Hvad sker der med kommunikationen, når mediestormen raser over de kommunale organisationer?', Ledelse i Morgen - Tidsskrift for pædagogisk ledelse, vol. 23, no. 3.

Kjeldsen, CC 2019, Hvad større internationale evalueringer (TIMSS og PIRLS) kan fortælle os om kvalitet i undervisningen. in Lærerkommissionens forskerpublikation. Danmarks lærerforening, København, pp. 82-91. <https://www.kl.dk/media/22541/bilag-8-laererkommissionens-forskerpublikation.pdf>

Displaying results 99551 to 99600 out of 390507

1992

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen