Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Hsieh, YT, Jylhä, A, Orso, V, Andolina, S, Hoggan, E, Gamberini, L & Jacucci, G 2019, 'Developing hand-worn input and haptic support for real-world target finding', Personal and Ubiquitous Computing, vol. 23, no. 1, pp. 117-132. https://doi.org/10.1007/s00779-018-1180-z

Højholt, C & Kousholt, D 2019, 'Developing knowledge through participation and collaboration: Research as mutual learning processes', Annual Review of Critical Psychology (Online), vol. 16, pp. 575-604. <https://discourseunit.com/annual-review/arcp-16-kritische-psychologie-2019/>

Marabello, D, Antoniotti, P, Benzi, P, Cariati, E, Lo Presti, L & Canepa, C 2019, 'Developing new SrI ₂ and β-d-fructopyranose-based metal–organic frameworks with nonlinear optical properties', Acta Crystallographica Section B: Structural Science, Crystal Engineering and Materials, vol. 75, no. 2, pp. 210-218. https://doi.org/10.1107/S2052520619001951

Prilop, CN, Weber, KE & Kleinknecht, M 2019, 'Developing Peer Feedback Expertise: Effects of a Face-to-Face Versus Video-Based Feedback Environment', Annual Meeting of the American Educational Research Association (AERA), Toronto, Canada, 11/03/2019.

Ryom, K, Stelter, R & Wikman, JM 2019, 'Developing self-concept through team sport and coaching/mentoring in an immigrant setting', FEPSAC 2019, Münster, Germany, 15/07/2019 - 20/07/2019.

Musaeus, LH 2019, 'Developing Student's Computational Thinking through Agent-Based Modeling in Secondary Education', ICER2019, Fifteenth annual ACM International Computing Education Research (ICER) conference, Toronto, ON, Canada, 12-14 August, 2019, Toronto, Canada, 12/08/2019 - 14/08/2019 pp. 345-346. https://doi.org/10.1145/3291279.3339430

Yilmaz, Z, Gottfredson, NC, Zerwas, SC, Bulik, CM & Micali, N 2019, 'Developmental Premorbid Body Mass Index Trajectories of Adolescents With Eating Disorders in a Longitudinal Population Cohort', Journal of the American Academy of Child and Adolescent Psychiatry, vol. 58, no. 2, pp. 191-199. https://doi.org/10.1016/j.jaac.2018.11.008

TA, S, K, H, DA, H & Davis, FM 2019, 'Developmental Stage-Specific Distribution of Macrophages in Mouse Mammary Gland', Frontiers in Cell and Developmental Biology, vol. 7, 250. https://doi.org/10.3389/fcell.2019.00250

Ali, A, Vasileva, S, Langguth, M, Alexander, S, Cui, X, Whitehouse, A, McGrath, JJ & Eyles, D 2019, 'Developmental Vitamin D Deficiency Produces Behavioral Phenotypes of Relevance to Autism in an Animal Model', Nutrients, vol. 11, no. 5, 1187. https://doi.org/10.3390/nu11051187

Fiorentini, M, Serale, G, Kokogiannakis, G, Capozzoli, A & Cooper, P 2019, 'Development and evaluation of a comfort-oriented control strategy for thermal management of mixed-mode ventilated buildings', Energy and Buildings, vol. 202, 109347. https://doi.org/10.1016/j.enbuild.2019.109347

Glerup Lauridsen, K, Watanabe, I, Nishisaki, A, Berg, RA, Niles, DE, Hunt, EA, Duval-Arnould, JM, Good, G, Cheng, A, Løfgren, B & Nadkarni, V 2019, 'Development and Evaluation of Standardized Communication For In-hospital Resuscitation', Circulation, vol. 140, 247. <https://www.ahajournals.org/doi/10.1161/circ.140.suppl_2.247>

Friis, RB, Hjollund, NH, Mejdahl, C, Pappot, H & Skuladottir, H 2019, 'Development and feasibility of the use of electronic symptom self-reporting as a tool for intensified disease monitoring in lung cancer in Denmark', Isoqol 2019, San Diego, United States, 20/10/2019 pp. S121-S121.

Khan, J, Kakosimos K.E., KE, Raaschou-Nielsen, O , Brandt, J , Jensen, SS , Ellermann, T & Ketzel, M 2019, 'Development and performance evaluation of new AirGIS – A GIS based air pollution and human exposure modelling system', Atmospheric Environment, vol. 198, pp. 102-121. https://doi.org/10.1016/j.atmosenv.2018.10.036

del Palacio Gonzalez, A , Jensen, KL, Højgaard, F, Lauritsen, H & Roliggaard, K 2019, 'Development and pilot testing of MApp: A mobile app that targets intrusive memories during dysphoria', 9th World Congress Cognitive and Behavioral Therapies , Berlin, Germany, 17/07/2019 - 20/07/2019.

Villumsen, S, Faxvaag, A & Nøhr, C 2019, Development and Progression in Danish eHealth Policies: Towards Evidence-Based Policy Making. in L Ohno-Machado & B Séroussi (eds), MEDINFO 2019: Health and Wellbeing e-Networks for All - Proceedings of the 17th World Congress on Medical and Health Informatics. vol. 264, IOS Press, Studies in Health Technology and Informatics, pp. 1075-1079, 17th World Congress on Medical and Health Informatics, MEDINFO 2019, Lyon, France, 25/08/2019. https://doi.org/10.3233/SHTI190390

van Lier, QDJ, Barros, AHC, Iversen, BV & Vereecken, H 2019, 'Development and Uncertainty Assessment of Pedotransfer Functions for Predicting Water Contents at Specific Pressure Heads', Vadose Zone Journal, vol. 18, no. 1, 190063. https://doi.org/10.2136/vzj2019.06.0063

Justesen, CG, Astals, S, Mortensen, JR, Thorsen, R, Koch, K, Weinrich, S, Triolo, JM & Hafner, SD 2019, 'Development and Validation of a Low-Cost Gas Density Method for Measuring Biochemical Methane Potential (BMP)', Water, vol. 11, no. 12, 2431. https://doi.org/10.3390/w11122431

Jørgensen, M, Savran, MM, Christakopoulos, C, Bek, T, Grauslund, J, Toft, PB, Ziemssen, F, Konge, L, Sørensen, TL & Subhi, Y 2019, 'Development and validation of a multiple-choice questionnaire-based theoretical test in direct ophthalmoscopy', Acta Ophthalmologica, vol. 97, no. 7, pp. 700-706. https://doi.org/10.1111/aos.14065

Diamantidou, D, Begou, O, Theodoridis, G, Gika, H, Tsochatzis, E, Kalogiannis, S, Kataiftsi, N, Soufleros, E & Zotou, A 2019, 'Development and validation of an ultra high performance liquid chromatography-tandem mass spectrometry method for the determination of phthalate esters in Greek grape marc spirits', Journal of Chromatography A, vol. 1603, pp. 165-178. https://doi.org/10.1016/j.chroma.2019.06.034

Sunde, PB & Sunde, P 2019, Development and variance components in single-digit addition strategies in year one. in UT Jankvist, M Van den Heuvel-Panhuizen & M Veldhuis (eds), Proceedings of the Eleventh Congress of the European Society for Research in Mathematics Education. European Society for Research in Mathematics Education, Utrecht, Eleventh Congress of the European Society for Research in Mathematics Education (CERME11), Utrecht, Netherlands, 06/02/2019.

Gligorescu, A, Toft, S, Hauggaard-Nielsen, H, Axelsen, JA & Nielsen, SA 2019, 'Development, growth and metabolic rate of Hermetia illucens larvae', Journal of Applied Entomology, vol. 143, no. 8, pp. 875-881. https://doi.org/10.1111/jen.12653

Ingvorsen, EB, Schnohr, C, Andersen, T, Lehrmann, L, Funding, E, Poulsen, LH, Holm, KB, Laursen, AL, Gerstoft, J & Bjorner, JB 2019, '"Development in well-being and social function among Danish hemophilia patients with HIV: a three-wave panel study spanning 24 years"', BMC Public Health, vol. 19, no. 1, 1714. https://doi.org/10.1186/s12889-019-8062-9

Guzman, JJL, Z. Sousa, D & Angenent, L 2019, 'Development of a bioelectrochemical system as a tool to enrich H2-producing syntrophic bacteria.', Frontiers in Microbiology, vol. 10, no. 110. https://doi.org/10.3389/fmicb.2019.00110

Ellegaard, MBB , Jensen, AB & Lomborg, K 2019, 'Development of a Cancer Self-management Education Programme for Women with Breast Cancer at the End of Primary Treatment', Journal of Cancer Education, vol. 34, no. 5, pp. 882-889. https://doi.org/10.1007/s13187-018-1390-0

Sølberg, J & Højgaard, T 2019, 'Development of a competency-based curriculum for science education in Denmark', Paper presented at European Science Education Research Association, Bologna, Italy, 26/08/2019 - 30/08/2019.

Barber, S, Bekker, H, Marti, J, Pavitt, S, Khambay, B & Meads, D 2019, 'Development of a Discrete-Choice Experiment (DCE) to Elicit Adolescent and Parent Preferences for Hypodontia Treatment', The Patient, vol. 12, no. 1, pp. 137-148. https://doi.org/10.1007/s40271-018-0338-0

Aagaard Kristensen, J, Balstrøm, T, Jones, RJA, Jones, A, Montanarella, L, Panagos, P & Breuning-Madsen, H 2019, 'Development of a harmonised soil profile analytical database for Europe: A resource for supporting regional soil management', SOIL, vol. 5, no. 2, pp. 289-301. https://doi.org/10.5194/soil-5-289-2019

Høyer, AS, Klint, KES, Fiandaca, G , Maurya, PK , Christiansen, AV, Balbarini, N, Bjerg, PL, Hansen, TB & Møller, I 2019, 'Development of a high-resolution 3D geological model for landfill leachate risk assessment', Engineering Geology, vol. 249, pp. 45-59. https://doi.org/10.1016/j.enggeo.2018.12.015

Tay, B, Stewart, TA, Davis, FM, Deuis, JR & Vetter, I 2019, 'Development of a high-throughput fluorescent no-wash sodium influx assay', PLoS One, vol. 14, no. 3, e0213751. https://doi.org/10.1371/journal.pone.0213751

Østergaard Mariager, C , Søvsø Szocska Hansen, E, Bech, SK, Nielsen, PF, Ringgaard, S , Kimose, H-H & Laustsen, C 2019, Development of a human heart sized perfusion system for metabolic imaging studies using hyperpolarized [1-13C]pyruvate MRI. in ISMRM 2019.

Whyte, J, Poulsen, I, Ni, P, Eskildsen, M & Guldager, R 2019, 'Development of a Measure of Nociception and Pain for Patients with Severe Brain Injury', pp. 34-34.

Rong, L & Aarnink, AJA 2019, 'Development of ammonia mass transfer coefficient models for the atmosphere above two types of the slatted floors in a pig house using computational fluid dynamics', Biosystems Engineering, vol. 183, no. July, pp. 13-25. https://doi.org/10.1016/j.biosystemseng.2019.04.011

Pietersen, PI, Konge, L, Madsen, KR, Bendixen, M, Maskell, NA, Rahman, N, Graumann, O & Laursen, CB 2019, 'Development of and Gathering Validity Evidence for a Theoretical Test in Thoracic Ultrasound', Respiration, vol. 98, no. 3, pp. 221-229. https://doi.org/10.1159/000500146

Fiorentini, M, Tartarini, F, Ledo Gomis, L, Daly, D & Cooper, P 2019, 'Development of an enthalpy-based index to assess climatic potential for ventilative cooling of buildings: An Australian example', Applied Energy, vol. 251, 113169. https://doi.org/10.1016/j.apenergy.2019.04.165

Larsen, JB, Madeleine, P & Arendt-Nielsen, L 2019, 'Development of a new bed-side-test assessing conditioned pain modulation: A test-retest reliability study', Scandinavian Journal of Pain, vol. 19, no. 3, pp. 565-574. https://doi.org/10.1515/sjpain-2018-0353

Ali, S, Daniel, E, Bryce, J, Ikiroma, A, Lewsey, J, Ross, R, Birkebæk, N, Krone, N, Darendeliler, F & Ahmed, F 2019, 'Development of an international benchmark for sick day episodes as a core clinical outcome in people with congenital adrenal hyperplasia', Hormone Research in Paediatrics, vol. 91, no. 1, P1-167, pp. 209-10. <https://www.karger.com/Article/Pdf/501868>

Pedersen, MM , Fink, PK , Kasch, H & Frostholm, L 2019, 'Development of an Internet-delivered educational video for acute whiplash injuries', Pilot and Feasibility Studies, vol. 5, no. 1. https://doi.org/10.1186/s40814-019-0445-2

Dehli, J 2019, 'Development of a novel percutaneous implant and investigation of the skin-implant interphase'.

Norrish, G, Ding, T, Field, E, Ziólkowska, L, Olivotto, I, Limongelli, G, Anastasakis, A, Weintraub, R, Biagini, E, Ragni, L, Prendiville, T, Duignan, S, McLeod, K, Ilina, M, Fernández, A, Bökenkamp, R, Baban, A, Kubuš, P, Daubeney, PEF, Sarquella-Brugada, G, Cesar, S, Marrone, C, Bhole, V, Medrano, C, Uzun, O, Brown, E, Gran, F, Castro, FJ, Stuart, G, Vignati, G, Barriales-Villa, R, Guereta, LG, Adwani, S, Linter, K, Bharucha, T, Garcia-Pavia, P, Rasmussen, TB, Calcagnino, MM, Jones, CB, De Wilde, H, Toru-Kubo, J, Felice, T, Mogensen, J, Mathur, S, Reinhardt, Z, O'Mahony, C, Elliott, PM, Omar, RZ & Kaski, JP 2019, 'Development of a Novel Risk Prediction Model for Sudden Cardiac Death in Childhood Hypertrophic Cardiomyopathy (HCM Risk-Kids)', JAMA cardiology, vol. 4, no. 9, pp. 918-927. https://doi.org/10.1001/jamacardio.2019.2861

Warner, D, Dettmann, F, Buitenhuis, AJ , Kargo, M, Hostrup Kjeldsen, AM, Nielsen, NH, Lefebvre, DM & Santschi, DE 2019, 'Development of a prediction equation for body weight change in early-lactating dairy cows by Fourier-transform infrared predicted fatty acid profiles in milk', Journal of Dairy Science, vol. 102, no. Supplement 1, 95, pp. 109-110. <https://www.adsa.org/Portals/0/SiteContent/Docs/Meetings/2019ADSA/2019ADSA_Abstract_Book.pdf?v20190715>

Kamari, A & Kirkegaard, PH 2019, 'Development of a rating scale to measuring the KPIs in the generation and evaluation of holistic renovation scenarios', I O P Conference Series: Earth and Environmental Science, vol. 294, no. 1, 012043. https://doi.org/10.1088/1755-1315/294/1/012043

Wadepohl, K, Blaha, T, Van Gompel, L, Duarte, ASR, Nielsen, CL, Saatkamp, H, Wagenaar, JA & Meemken, D 2019, 'Development of a simplified on-farm animal health and welfare benchmarking tool for pig herds', Berliner und Muenchener Tieraerztliche Wochenschrift, vol. 132, no. 11-12, pp. 504-512. https://doi.org/10.2376/0005-9366-18088

Zhang, K, Liu, X, Ata-Ul-Karim, ST, Lu, J, Krienke, B, Li, S, Cao, Q, Zhu, Y, Cao, W & Tian, Y 2019, 'Development of chlorophyll-meter-index-based dynamic models for evaluation of high-yield japonica rice production in Yangtze river reaches', Agronomy, vol. 9, no. 2, 106;. https://doi.org/10.3390/agronomy9020106

Blandfort, SS , Gregersen, M, Rahbek, K, Juul, S & Damsgaard, EMS 2019, 'Development of delirium is associated with consumption of antipsychotic and anti-dementia drugs', European Delirium Association Meeting, Edinburgh, United Kingdom, 05/09/2019 - 06/09/2019.

Roell, YE , Peng, Y , Beucher, AM , Greve, MB & Greve, MH 2019, 'Development of hierarchical terron workflow based on gridded data – A case study in Denmark', Joint Workshop for Digital Soil Mapping and GlobalSoilMap, Santiago, Chile, 13/03/2019 - 16/03/2019.

Ingvardsen, CR , Massange-Sánchez, JA, Borum, F, Uauy, C & Gregersen, PL 2019, 'Development of mlo-based resistance in tetraploid wheat against wheat powdery mildew', Theoretical and Applied Genetics, vol. 132, no. 11, pp. 3009-3022. https://doi.org/10.1007/s00122-019-03402-4

Meyer, LE, Gummesson, A, Kragl, U & von Langermann, J 2019, 'Development of Ionic Liquid-Water-Based Thermomorphic Solvent (TMS)-Systems for Biocatalytic Reactions', Biotechnology Journal, vol. 14, no. 10, 1900215. https://doi.org/10.1002/biot.201900215

Zhang, LX, Sun, XM, Xu, ZP & Liu, RT 2019, 'Development of Multifunctional Clay-Based Nanomedicine for Elimination of Primary Invasive Breast Cancer and Prevention of Its Lung Metastasis and Distant Inoculation', ACS Applied Materials & Interfaces, vol. 11, no. 39, pp. 35566-35576. https://doi.org/10.1021/acsami.9b11746

Baagøe, SK, Kofoed-Hansen, M, Poulsen, I & Riberholt, CG 2019, 'Development of muscle contractures and spasticity during subacute rehabilitation after severe acquired brain injury: a prospective cohort study', Brain Injury, vol. 33, no. 11, pp. 1460-1466. https://doi.org/10.1080/02699052.2019.1646433

Nielsen, LDF 2019, 'Development of Small Molecule Sensors for Point-of-Care Testing'.

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