Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Jensen, JM, Duvall, TL, Jacobsen, BH & et, A 2001, 'Imaging an emerging active region with helioseismic tomography', Astrophys. Journ., vol. 553, pp. 193-196.

Jensen, JM, Duvall, TL, Jacobsen, BH & Christensen-Dalsgaard, J 2001 'Imaging an emerging active region with helioseismic tomography' pp. 193-193.

Varea, E, Ponsoda, X, Molowny, A, Danscher, G & Lopez-Garcia, C 2001, 'Imaging synaptic zinc release in living nervous tissue', J. Neurosci. Methods, vol. 110, pp. 57-63.

Eriksen-Benros, J 2001, 'I magtens vertigo: Parallelisering og forgrening i Jens-Martin Eriksens Vinter ved daggry', Tidsskriftet SPRING, no. 17, pp. 89-103. <http://docs.google.com/viewer?a=v&q=cache:-R1P-aOKF_gJ:www.forlagetspring.dk/08benros.pdf+Parallelisering+og+forgrening+i+Jens-Martin+Eriksens+Vinter+ved+daggry&hl=da&gl=dk&pid=bl&srcid=ADGEEShbGEQLCjaXi0GWqEt_kRUxsy-6-o_P-n69vm3_yvlCuk-fSn1iimGfu_uGsJbtht9GPoTLlLTdCiXmnIxBnKlvFz9GnKyz5xqxjT1SZDFpSX1Cuik0YbYId00yCFrdbIOIxIhG&sig=AHIEtbQijIm0hm0yUa0c9Esmd2OfttU1XA>

Bøggild, CE, Knudsen, NT & Ahlstrøm, AP 2001, 'Imersuaq - vandkraftpotentiale i Grønland', Geologisk Nyt, no. 3/01, pp. 13-16.

Paludan, PS & Andersen, V (ed.) 2001, Immigranter fra en anden verden. in Religionsvidenskabelige sonderinger: Festskrift i anledning af et 10 års jubilæum. Aarhus Universitetsforlag, Århus, pp. 136-152.

Larsen, LE, Viuff, B, Tjørnehøj, K & Røntved, CM 2001, 'Immunitetsstyring i slagtekalvebesætninger og orientering om aktuelle forskningsaktiviteter', Kalveproducenten, vol. 19, no. 3, pp. 1-6.

Henrik, H, Kwon, T-H, Vinnikova, AK, Masilamini, S, Frøkiær, J, Knepper, MA & Nielsen, S 2001, 'Immunocytichemical and immunoelectron microscopical localization of alfa, beta and gamma-ENac in rat kidney', Am J Physiol, Renal Fluid and Electrolyte Physiol, vol. 280, pp. 1093-1106.

Hager, H, Kwon, T-H, Vinnikova, AK, Masilamani, S, Brooks, HL, Frokiaer, J, Knepper, MA & Nielsen, S 2001, 'Immunocytochemical and immunoelectron microscopic localization of alpha-, beta-, and gamma-ENaC in rat kidney', Am. J. Physiol., vol. 280, no. 6, pp. 1093-1106.

Elkjær, M-L, Nejsum, LN, Gresz, V, Kwon, TH, Jensen, UB , Frøkiaer, J & Nielsen, S 2001, 'Immunolocalization of aquaporin-8 in rat kidney, gastrointestinal tract, testis, and airways', American Journal of Physiology: Renal Physiology, vol. 281, no. 6, pp. F1047-57.

Andersen, SK , Toft, P & Tønnesen, EK 2001, 'Immunological aspects of major accidental trauma with special reference to cytokines: A review', Placebo, vol. 2, no. 4, pp. 123-136.

Deleuran, B, Petersen, J, Friis, J (ed.), Junker, P (ed.), Manniche, C (ed.) & Stengaard-Pedersen, K (ed.) 2001, Immunologi og inflammation. in Reumatologi. FADL's Forlag, København, pp. 81-94.

Wiggers, H , Bøttcher, M , Egeblad, H , Mølgaard, H , Nielsen, TT & Bøtker, HE 2001, 'Impact daily life myocardial ischemia in patients with chronic reversible and irreversible myocardial dysfunction', American Journal of Cardiology, vol. 89, no. 1, pp. 22-28.

Søgaard, P, Kim, WY , Jensen, HK , Mortensen, P , Pedersen, AK , Kristensen, BØ & Egeblad, H 2001, 'Impact of acute biventricular pacing on left ventricular performance and volumes in patients with severe heart failure. A tissue Doppler and three dimensional echocardiographic study', Cardiology (Switzerland), vol. 95, no. 4, pp. 173-182.

Kim, WY, Stuber, M, Kissinger, KV, Andersen, NT, Manning, WJ & Botnar, RM 2001, 'Impact of Bulk Cardiac Motion on Right Coronary MR Angiography and Vessel Wall Imaging', Journal of Magnetic Resonance Imaging, vol. 14, pp. 383-390.

Schropp, L , Wenzel, A & Kostopoulos, L 2001, 'Impact of conventional tomography on prediction of the appropriate implant size', Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, vol. 92, no. 4, pp. 458-463.

Jørgensen, RN & Jørgensen, JR 2001, Impact on grain quality when nitrogen is ?sensor applied? by the ?hydro precise system? in G Grenier & S Blackmore (eds), Proceedings of the Third European Conference on Precision Agriculture. vol. 2, pp. 929-934, European Conference on Precision Agriculture, 18/12/2010.

Sørensen, AH 2001, 'Impact-parameter dependence of the relativistic photoeffect and other high-energy photoprocesses', Physical Review A, vol. 64, pp. 012703-1-11.

Fornitz, GG, Nielsen, P, Amtorp, O, Kassis, E, Abildgård, U, Sloth, C, Winther, K, Ørskov, H, Dalsgård, J & Husted, S 2001, 'Impaired fibrinolysis determines the outcome of percutaneous transluminal coronary angioplasty (PTCA)', Eur. J. Clin. Invest., vol. 31, pp. 586-592.

Fornitz, GG, Nielsen, P, Amtorp, O, Kassis, E, Abildgard, U, Sloth, C, Winther, K, Ørskov, H, Dalsgard, J & Husted, S 2001, 'Impaired fibrinolysis determines the outcome of percutaneus transluminal coronary angioplasty', Eur. J. Clin. Invest., vol. 31, no. 7, pp. 586-592.

Ridgway, MC, Glover, CJ, Desnica-Frankovic, ID, Furic, K, Yu, KM, Forna, GJ, Clerc, C, Hansen, JL & Nylandsted Larsen, A 2001, 'Implantation-induced disorder in amorphous Ge: production and relaxation', Nucl. Instrum. and Methods in Physics Research B, vol. 175-177, pp. 21-25.

Schers, H, Wensing, M, Huijsmans, Z, van Tulder, M & Grol, R 2001, 'Implementation barriers for general practice guidelines on low back pain a qualitative study.', Spine, vol. 26, no. 15. <http://www.scopus.com/inward/record.url?scp=1842691397&partnerID=8YFLogxK>

Hansen, JG & Kapsa, J 2001, Implementation of an Internet based decision support system for integrated potato late blight management in Poland. in European Association for Potato Research, Pathology Section Meeting. pp. 286-286, Implementation of an Internet based decision support system for integrated potato late blight management in Poland, Poznan, Poland, 10/07/2001.

Jensen, B & Harmsen, H 2001, 'Implementation of success factors in new product development: The missing link', European Journal of Innovation Management, vol. 4, no. 1, pp. 37-52.

Iversen, TM 2001, 'Implementation of the Water Framework Directive in Denmark', Newsletter - ECRR, European Centre for River Restoration, vol. 5, no. 2, pp. 1-2.

Riis, JO, Hildebrandt, S, Andreasen, MM & Johansen, J 2001, 'Implementing change: lessons from five development projects', International Journal of Technology Management, vol. 22, no. 1-3, pp. 13-27. <http://elin.lub.lu.se/link2elin?genre=article&issn=02675730&year=2001&volume=22&issue=1-2-3&collection=ABI&pages=13-27&resid=c7373a56b8f1ef0f882028b51fe3820c&lang=dk>

Eriksen, J 2001, 'Implications of grazing by sows for nitrate leaching from grassland and the succeeding cereal crop', Grass and Forage Science, vol. 56, pp. 317-322.

Korsgaard, M & Kühn, BF 2001, 'Imponerende forædlingsarbejde i Tjekkiet', Frugt & Bær, vol. 30, no. 11, pp. 284-286.

Mertz, O, Lykke, AM & Reenberg, A 2001, 'Importance And Seasonality of Vegetable Consumption And Marketing In Burkina Faso', Economic Botany, vol. 55, no. 2, pp. 276-289. <http://www.jstor.org/stable/4256428>

Hammershøj, M & Qvist, KB 2001, 'Importance of hen age and egg storage time for egg albumen foaming', LWT - Food Science and Technology, vol. 34, no. 2, pp. 118-120.

Knudsen, C, Wieden, HJ & Rodnina, MV 2001, 'Importance of structural transitions of the switch II region for the functions of elongation factor Tu on the ribosome', Journal of Biological Chemistry, vol. 276, pp. 22183-22190.

Hansen, M, Busse, M & Andreasen, PA 2001, 'Importance of the amino acid composition of the shutter region of plasminogen activator inhibitor-1 for its transition to latent and substrate forms', FEBS journal, vol. 268, pp. 6274-6283.

Clausen, JD, McIntosh, DB, Woolley, DG & Andersen, JP 2001, 'Importance of Thr-353 of the conserved phosphorylation loop of the sarcoplasmic reticulum Ca²⁺-ATPase in MgATP binding and catalytic activity', Journal of Biological Chemistry, vol. 276, pp. 35741-35750.

Andersen, JP , Sørensen, TL-M, Povlsen, K & Vilsen, B 2001, 'Importance of transmembrane segment M3 of the sarcoplasmic reticulum Ca²⁺-ATPase for control of the gateway to the Ca²⁺-sites', Journal of Biological Chemistry, vol. 276, pp. 23312-23321.

Boertmann, D & Mosbech, A 2001, Important Summer Concentrations of Seaducks in West Greenland: An Input to Oil Spill Sensitivity Mapping. NERI Technical Report, vol. 345, National Environmental Research Institute. <http://www.dmu.dk/1_viden/2_Publikationer/3_fagrapporter/rapporter/FR345.pdf>

Apyan, AB, Avakian, RO, Darbinian, SM, Ispirian, KA, Taroian, SP, Mikkelsen, U & Uggerhoj, E 2001, 'Improved crystal method for photon beam linear polarization measurement at high energies', Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, vol. 173, no. 1, pp. 149-153. https://doi.org/10.1016/S0168-583X(00)00173-7

Jensen, MØJ, AA, F & AP, Y 2001, 'Improved in vitro quantification of the force exerted by the papillary muscle on the left ventricular wall: three dimensional force vector measurement system.', Biomed Eng., vol. 29, no. 5, pp. 406-413.

Sidaros, K, Andersen, IK, Gesmar, H, Rostrup, E & Larsson, HB 2001, 'Improved perfusion quantification in FAIR imaging by offset correction', Magnetic Resonance in Medicine, vol. 46, no. 1, pp. 193-7.

Murata, R, Siemann, DW, Overgaard, J & Horsman, MR 2001, 'Improved tumor response by combining radiation and the vascular-damaging drug 5,6-dimethylxanthenone-4-acetic acid', Radiation Research, vol. 156, no. 5 pt 1, pp. 503-509. <http://www.ncbi.nlm.nih.gov/pubmed/?term=Improved+tumor+response+by+combining+radiation+and+the+vascular-damaging+drug+5%2C6-dimethylxanthenone-4-acetic+acid>

Steenfeldt, S & Pettersson, D 2001, 'Improvements in nutrient digestibility and performance of broiler chickens fed a wheat-and-rye-based diet supplemented with enzymes', Journal of Animal and Feed Sciences, vol. 10, pp. 143-157.

Winther, M, Lohmann-Hansen, A (ed.) & Nielsen, J (ed.) 2001, 'Improving Fuel Statistics for Danish Aviation', ISP's Skriftserie, vol. 255, pp. 361-371.

Winther, M 2001, Improving Fuel Statistics for Danish Aviation. NERI Technical Report, vol. 387, National Environmental Research Institute. <http://www.dmu.dk/1_viden/2_Publikationer/3_fagrapporter/rapporter/FR387.pdf>

Horsman, MR, Murata, R & Overgaard, J 2001, 'Improving local tumor control by combining vascular targeting drugs, mild hyperthermia and radiation', Acta Oncologica, vol. 40, no. 4, pp. 497-503. <http://www.ncbi.nlm.nih.gov/pubmed/?term=11504310>

Madsen, MH, Kirk, HG, Sørensen, KK & Labouriau, R 2001, Improving potato starch applicability. in Øforsk workshop. pp. 13-13, Improving potato starch applicability, Mölle, Sweden, 07/06/2001.

Sørensen, LU & Gulman, NC 2001, Improving Psychoparmacotherapy in nursing homes.

Jelantik, IG, Madsen, J, Hvelplund, T & Weisbjerg, MR 2001, Improvong calf performance by supplementation in Bali cattle grazing commununal pastures in West Timor, Indonesia. in Proceedings 28th TSAP Conference, Morogoro, 7-9th August, 2001. pp. 289-303, Improvong calf performance by supplementation in Bali cattle grazing commununal pastures in West Timor, Indonesia, 18/12/2010.

Glémin, S, Bataillon, T, Ronfort, J, Mignot, A & Olivieri, I 2001, 'Inbreeding depression in small populations of self-incompatible plants', Genetics, vol. 159, no. 3, pp. 1217-29.

Lindholm, J, Juul, S , Jørgensen, JOL , Astrup, J, Bjerre, P, Feldt-Rasmussen, U, Hagen, C, Jørgensen, J, Kosteljanetz, M, Kristensen, L, Laurberg, P, Schmidt, K & Weeke, J 2001, 'Incidence and late prognosis of cushing's syndrome: a population based study', Journal of Clinical Endocrinology and Metabolism, vol. 86, no. 1, pp. 117-123. https://doi.org/10.1210/jcem.86.1.7093

Knudsen, B, Hindhede, J , Kudahl, AB & Sørensen, JT 2001, Incidence of milk fever and description of applied conrol strategies for preventing milk fever/subclinical hypocalcaemia in large dairy herds. in 11th ICPD, 12-16 August, Frederiksberg, Denmark. pp. 107-107, Incidence of milk fever and description of applied conrol strategies for preventing milk fever/subclinical hypocalcaemia in large dairy herds, 18/12/2010.

Madsen, J, Kuijken, E, Kuijken-Verscheure, C, Hansen, F & Cottaar, F 2001, 'Incidents of Neckband Icing and Consequences for Body Condition and Survival of Pink-Footed Geese Anser brachyrhynchus', Wildlife Biology, vol. 7, no. 1, pp. 49-53.

Displaying results 346201 to 346250 out of 391348

6925

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen