Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Wang, Y, Palmfeldt, J , Gregersen, N, Makhov, AM, Conway, JF, Wang, M, McCalley, SP, Basu, S, Alharbi, H, St Croix, C, Calderon, MJ, Watkins, S & Vockley, J 2019, 'Mitochondrial fatty acid oxidation and the electron transport chain comprise a multifunctional mitochondrial protein complex', Journal of Biological Chemistry, vol. 294, no. 33, pp. 12380-12391. https://doi.org/10.1074/jbc.RA119.008680

Zervas, A , Petersen, G & Seberg, O 2019, 'Mitochondrial genome evolution in parasitic plants', BMC Evolutionary Biology, vol. 19, no. 1, 87. https://doi.org/10.1186/s12862-019-1401-8

Paiva, ALS, Passaia, G, Lobo, AKM, Jardim-Messeder, D, Silveira, JAG & Margis-Pinheiro, M 2019, 'Mitochondrial glutathione peroxidase (OsGPX3) has a crucial role in rice protection against salt stress', Environmental and Experimental Botany. https://doi.org/10.1016/j.envexpbot.2018.10.027

Jespersen, NRV 2019, 'Mitochondrial modulation strategies to attenuate ischemia reperfusion injury in non-diabetic and diabetic individuals'.

Tjørnild, MJ , Skov, SN , Røpcke, DM , Ilkjær, C, Rasmussen, J, Couetil, J-P & Nielsen, SL 2019, 'Mitral annuloplasty ring with selective flexibility for septal-lateral contraction and remodelling properties', Interactive Cardiovascular and Thoracic Surgery, vol. 28, no. 1, pp. 65-70. https://doi.org/10.1093/icvts/ivy194

Lieberoth, A 2019, Mixed methods I aktionsforskning: design, analyse og forskeres blinde vinkler. in M Sonne Kam Sunesen (ed.), Aktionsforskning indefra og udefra. Dafolo, pp. 143.

Lieberoth, A 2019, Mixed methods som aktionsforskning: design, analyse og forskeres blinde vinkler. in MSK Sunesen (ed.), Aktionsforskning: indefra og udefra. Dafolo, Frederikshavn, Undervisning og læring, pp. 143-162.

Stoynov, V, Valkova-Jarvis, Z, Mihaylova, D, Iliev, G, Mihovska, A & Poulkov, V 2019, 'Mixed Waveform Numerology for Performance Enhancement in High Mobility Wireless Networks', Paper presented at International Symposium on Wireless Personal Multimedia Communications , Lisbon, Portugal, 24/11/2019 - 27/11/2019. https://doi.org/10.1109/WPMC48795.2019.9096085

Passeggeri, R & Veraart, AED 2019, 'Mixing Properties of Multivariate Infinitely Divisible Random Fields', Journal of Theoretical Probability, vol. 32, no. 4, pp. 1845-1879. https://doi.org/10.1007/s10959-018-0864-7

Nørby, S 2019, 'Mnemonic emotion regulation: Possibilities and limitations', 21st conference of the European Society for Cognitive Psychology, Playa de las Américas, Spain, 25/09/2019 - 28/09/2019 pp. 247-247.

Nørby, S 2019, 'Mnemonic emotion regulation: A three-process model', Cognition and Emotion, vol. 33, no. 5, pp. 959-975. https://doi.org/10.1080/02699931.2018.1523137

Zhang, M, Zheng, T, Sheng, B, Wu, F, Zhang, Q, Wang, W, Shen, J, Zhou, N & Sun, Y 2019, 'Mn ²⁺ complex-modified polydopamine- and dual emissive carbon dots based nanoparticles for in vitro and in vivo trimodality fluorescent, photothermal, and magnetic resonance imaging', Chemical Engineering Journal, vol. 373, pp. 1054-1063. https://doi.org/10.1016/j.cej.2019.05.107

Hansen, HR & Kofoed, J 2019, 'Mobbeforskere: Der findes ikke onde børn eller onde skærme - der findes onde mønstre', Politiken, debatsektionen.

Valentin, K & Niraula, A 2019, 'Mobile brains and the question of 'deskilling: Highly educated South Asian migrants in Denmark', Nordic Journal of Migration Research, vol. 9, no. 1, pp. 19-35. https://doi.org/10.2478/njmr-2019-0008

Bakker, P 2019, 'Mobilian Trade Language: Phrasebook & Lexicon. By David V. Kaufman. Chicago: Exploration Press, 2017. Pp. 72. $11.99 (paper). ISBN: 978-0999548608.', International Journal of American Linguistics, vol. 85, no. 2, pp. 271-272. https://doi.org/10.1086/701804

Pulles, NJ, Ellegaard, C, Schiele, H & Kragh, H 2019, 'Mobilising supplier resources by being an attractive customer: Relevance, status and future research directions', Journal of Purchasing and Supply Management, vol. 25, no. 3, 100539. https://doi.org/10.1016/j.pursup.2019.100539

Moberg, KR, Aall, C, Dorner, F, Reimerson, E, Ceron, JP, Sköld, B, Sovacool, BK & Piana, V 2019, 'Mobility, food and housing: responsibility, individual consumption and demand-side policies in European deep decarbonisation pathways', Energy Efficiency, vol. 12, no. 2, pp. 497-519. https://doi.org/10.1007/s12053-018-9708-7

Jørgensen, TSH, Siersma, V, Lund, R & Nilsson, CJ 2019, 'Mobility limitation as determinant of primary care use and ambulatory care sensitive conditions', European Geriatric Medicine, vol. 10, no. 1, pp. 53-60. https://doi.org/10.1007/s41999-018-0149-9

Wang, H, Gao, G, Xia, Q & Zhang, X 2019, Modal Dynamics and Analysis of a Vertical Stretch-Retractable Continuum Manipulator with Large Deflection. in 2019 International Conference on Robotics and Automation, ICRA 2019., 8794172, IEEE, pp. 6258-6264, 2019 International Conference on Robotics and Automation , Montreal, Canada, 20/05/2019. https://doi.org/10.1109/ICRA.2019.8794172

Filsoof, OT, Hansen, MH, Yde, A, Bøttcher, P & Zhang, X 2019, Modal Dynamics of a Three-Bladed Tri-Rotor Wind Turbine. in A Zingoni (ed.), Advances in Engineering Materials, Structures and Systems: Innovations, Mechanics and Applications - Proceedings of the 7th International Conference on Structural Engineering, Mechanics and Computation, 2019. CRC Press, Leiden, pp. 681-686, 7th International Conference on Structural Engineering, Mechanics and Computation, Cape Town, South Africa, 02/09/2019. https://doi.org/10.1201/9780429426506

Brincker, R, Olsen, P, Amador, S, Juul, MØØ, Malekjafarian, A & Ashory, M 2019, 'Modal participation in multiple input Ibrahim time domain identification', Mathematics and Mechanics of Solids, vol. 24, no. 1, pp. 168-180. https://doi.org/10.1177/1081286517733034

Tarpø, MG , Vigsø, M & Brincker, R 2019, Modal Truncation in Experimental Modal Analysis. in M Mains & BJ Dilworth (eds), Topics in Modal Analysis and Testing, Volume 9 - Proceedings of the 36th IMAC, A Conference and Exposition on Structural Dynamics 2018: Proceedings of the 36th IMAC, A Conference and Exposition on Structural Dynamics 2018. vol. 9, Springer, Conference Proceedings of the Society for Experimental Mechanics Series, no. 9, vol. 2018, pp. 143-152, IMAC - XXXVI CONFERENCE & EXPOSITION ON STRUCTURAL DYNAMICS, Orlando, Florida, United States, 12/02/2018. https://doi.org/10.1007/978-3-319-74700-2_15

Erichsen, AC, Closter, RM, Hansen, JW , Carstensen, J , Timmermann, K , Christensen, JH & Tornbjerg, H 2019, Modelanalyser og hydrografisk rapportering. Afsluttende rapport. DHI. <http://dce.au.dk/fileadmin/dce.au.dk/Udgivelser/Oevrige_udgivelser/Modeller_i_overvaagningen_2018.pdf>

Madsen, E, Skov Rosenlund, O, Brandt, D & Zhang, X 2019, Model-Based On-line Estimation of Time-Varying Nonlinear Joint Stiffness on an e-Series Universal Robots Manipulator. in 2019 International Conference on Robotics and Automation (ICRA) ., 8793935, IEEE, pp. 8408-8414, 2019 International Conference on Robotics and Automation , Montreal, Canada, 20/05/2019. https://doi.org/10.1109/ICRA.2019.8793935

Møller, A & Torp, MT 2019, Model-based testing of breaking changes in Node.js libraries. in S Apel, M Dumas, A Russo & D Pfahl (eds), ESEC/FSE 2019 - Proceedings of the 2019 27th ACM Joint Meeting European Software Engineering Conference and Symposium on the Foundations of Software Engineering. Association for Computing Machinery, pp. 409-419, 27th ACM Joint European Software Engineering Conference and Symposium on the Foundations of Software Engineering, ESEC/FSE 2019, Tallinn, Estonia, 26/08/2019. https://doi.org/10.1145/3338906.3338940

Olesen, JE & Pullens, JWM 2019, 'Modelbaseret prognose for høstdato i korn for 2019', No. 2019-760-001085, 7 p., Jul 15, 2019..

Holm, H & Jørgensen, P 2019, 'Model categories of quiver representations', Advances in Mathematics, vol. 357, 106826. https://doi.org/10.1016/j.aim.2019.106826

Bloemen, V, Duret-Lutz, A & van de Pol, J 2019, 'Model checking with generalized Rabin and Fin-less automata', International Journal on Software Tools for Technology Transfer, vol. 21, no. 3, pp. 307-324. https://doi.org/10.1007/s10009-019-00508-4

Zhang, W, Jansson, PE, Sigsgaard, C, McConnell, A, Jammet, MM, Westergaard-Nielsen, A, Lund, M, Friborg, T, Michelsen, A & Elberling, B 2019, 'Model-data fusion to assess year-round CO ₂ fluxes for an arctic heath ecosystem in West Greenland (69°N)', Agricultural and Forest Meteorology, vol. 272-273, no. July, pp. 176-186. https://doi.org/10.1016/j.agrformet.2019.02.021

Ring, T & Kellum, JA 2019, 'Modeling Acid-Base by Minimizing Charge-Balance', ACS Omega, vol. 4, no. 4, pp. 6521-6529. https://doi.org/10.1021/acsomega.9b00270

Madhiarasan, M, Keerthana, T, Shakeel, M, Rakesh, S & Gopal, R 2019, 'Modeling and implementation IoT based transmission line monitoring, protection and control', Water and Energy International, vol. 62r, no. 5, pp. 35-39.

Safi, S, Ahmadieh, H, Katibeh, M, Yaseri, M, Nikkhah, H, Karimi, S, Nourinia, R, Tivay, A, Zareinejad, M, Azarmina, M, Ramezani, A, Moradian, S, Dehghan, MH, Daftarian, N, Abbasi, D, Eshghi Fallah, A & Kheiri, B 2019, 'Modeling a Telemedicine Screening Program for Diabetic Retinopathy in Iran and Implementing a Pilot Project in Tehran Suburb', Journal of Ophthalmology, vol. 2019, 2073679. https://doi.org/10.1155/2019/2073679

Tieppo, RC, Romanelli, TL, Milan, M, Sørensen, CAG & Bochtis, D 2019, 'Modeling cost and energy demand in agricultural machinery fleets for soybean and maize cultivated using a no-tillage system', Computers and Electronics in Agriculture, vol. 156, pp. 282-292. https://doi.org/10.1016/j.compag.2018.11.032

Iglesias, RS, Romio, C, Melo, CL, Musse, APS, do Rosário, F & Oldenburg, CM 2019, 'Modeling CO₂ flow in support of a shallow subsurface controlled leakage field test', Greenhouse Gases: Science and Technology, vol. 9, no. 5, pp. 1027-1042. https://doi.org/10.1002/ghg.1917

Bennedsen, M , Hillebrand, E & Koopman, SJ 2019 'Modeling, Forecasting, and Nowcasting U.S. CO2 Emissions Using Many Macroeconomic Predictors' CREATES Research Paper, no. 2019-21, Aarhus.

Sanchez, EML, Rosendahl, L & Pedersen, TH 2019, 'Modeling of thermochemically liquefied biomass products and heat of formation for process energy assessment', Applied Energy, vol. 254, 113654. https://doi.org/10.1016/J.APENERGY.2019.113654

Shreya, S & Kaushik, BK 2019, 'Modeling of Voltage-Controlled Spin–Orbit Torque MRAM for Multilevel Switching Application', IEEE Transactions on Electron Devices, vol. 67, no. 1, 19312204, pp. 90. https://doi.org/10.1109/TED.2019.2951684

Veraart, AED 2019, 'Modeling, simulation and inference for multivariate time series of counts using trawl processes', Journal of Multivariate Analysis, vol. 169, pp. 110-129. https://doi.org/10.1016/j.jmva.2018.08.012

Christensen, JH & Børglum, AD 2019, 'Modeling the cooperativity of schizophrenia risk genes', Nature Genetics, vol. 51, no. 10, pp. 1434-1436. https://doi.org/10.1038/s41588-019-0508-6

Darko, D, Trolle, D, Asmah, R, Bolding, K, Adjei, KA & Odai, SN 2019, 'Modeling the impacts of climate change on the thermal and oxygen dynamics of Lake Volta', Journal of Great Lakes Research, vol. 45, no. 1, pp. 73-86. https://doi.org/10.1016/j.jglr.2018.11.010

Donnellan, L 2019, 'Modeling the Rise of the City: Early Urban Networks in Southern Italy', Frontiers in Digital Humanities, vol. 6, 15, pp. 1-19. https://doi.org/10.3389/fdigh.2019.00015

Hankerson, BR, Schierhorn, F, Prishchepov, AV, Dong, C, Eisfelder, C & Mueller, D 2019, 'Modeling the spatial distribution of grazing intensity in Kazakhstan', PLoS One, vol. 14, no. 1. https://doi.org/10.1371/journal.pone.0210051

Maar, M , Larsen, J , Dahl, K & Riemann, B 2019, 'Modellering af miljøeffekter fra havbrug og mulig næringsstofkompensation ved hjælp af miljømuslinger', 20. Danske Havforskermøde, Odense, Denmark, 23/01/2019 - 25/01/2019. <https://www.dhm2019.dk/>

Engesgaard, P, Petersen, RJ, Karlsson, IB, Christiansen, IMV, Kjaergaard, C & Højbjerg, AL 2019, 'Modellering af vandstrømning i lavbundsområder', Vand & Jord, vol. 26, no. 1, pp. 37-39.

Stutz, HH, Fu, S & Yang, Z 2019, Modellierung von großen Deformationen des Boden-Bauwerk-Kontakts in expliziten numerischen Simulationen. in Numerik in der Geotechnik. Bundesanstalt für Wasserbau, Karlsruhe, pp. 43-48, Numerik in der Geotechnik, Karlsruhe, Germany, 09/10/2019. <https://henry.baw.de/handle/20.500.11970/106582>

Gerwig, H, Hellebrandt, P, Jakobs, H, Janicke, U, Ketzel, M, Schmidt, W, Wirtz, K & Lorentz, H 2019, 'Modellierung von Ultrafeinpartikeln am und um den Flughafen Frankfurt/Main', 3. Symposium "Ultrafeine Partikel in der Außenluft und in Innenräumen", Berlin, Germany, 19/09/2019 - 20/09/2019.

Manevski, K , Børgesen, CD, Li, X, Andersen, MN, Zhang, X, Shen, Y & Hu, C 2019, 'Modelling agro-environmental variables under data availability limitations and scenario managements in an alluvial region of the North China Plain', Environmental Modelling & Software, vol. 111, no. January, pp. 94-107. https://doi.org/10.1016/j.envsoft.2018.10.001

Kullolli, B, Cuéllar, P, Baessler, M & Stutz, HH 2019, Modelling and calibration for cyclic soil-structure interface behaviour. in E Ibraim & A Tarantino (eds), 7th International Symposium on Deformation Characteristics of Geomaterials, IS-Glasgow 2019., 13007, EDP Sciences, E3S Web of Conferences, vol. 92, 7th International Symposium on Deformation Characteristics of Geomaterials, Glasgow, United Kingdom, 26/06/2019. https://doi.org/10.1051/e3sconf/20199213007

Somerville, GJ , Melander, B , Kudsk, P & Mathiassen, SK 2019, 'Modelling annual grass weed seed dispersal in winter wheat, when influenced by hedges and directional wind', Ecological Modelling, vol. 410, 108729. https://doi.org/10.1016/j.ecolmodel.2019.108729

Curci, G, Alyuz, U, Barò, R, Bianconi, R, Bieser, J, H. Christensen, J, Colette, A, Farrow, A, Francis, X, Jiménez-Guerrero, P, Im, U, Liu, P, Manders, A, Palacios-Peña, L, Prank, M, Pozzoli, L, Sokhi, R, Solazzo, E, Tuccella, P, Unal, A, Vivanco, MG, Hogrefe, C & Galmarini, S 2019, 'Modelling black carbon absorption of solar radiation: Combining external and internal mixing assumptions', Atmospheric Chemistry and Physics, vol. 19, no. 1, pp. 181-204. https://doi.org/10.5194/acp-19-181-2019

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