Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Schilhab, T 2015, 'Re-live and learn Interlocutor-induced elicitation of phenomenal experiences in learning offline.', Progress in Biophysics & Molecular Biology, vol. 119, no. 3, pp. 649-660.

Skibsted, E & Ito, K 2015, 'Rellich’s theorem, limiting absorption principle and radiation condition on manifold with ends', RIMS Kokyuroku, vol. 1975, pp. 117-126. <http://www.kurims.kyoto-u.ac.jp/~kyodo/kokyuroku/contents/pdf/1975-10.pdf>

Supur, M, Yurtsever, A & Akbey, Ü 2015, 'Remarkable enhancement of ambient-air electrical conductivity of the perylenediimide π-stacks isolated in the flexible films of a hydrogen-bonded polymer', RSC Advances, vol. 5, no. 79, pp. 64240-64246. https://doi.org/10.1039/c5ra12169h

Sauni, R, Verbeek, JH, Uitti, J, Jauhiainen, M, Kreiss, K & Sigsgaard, T 2015, 'Remediating buildings damaged by dampness and mould for preventing or reducing respiratory tract symptoms, infections and asthma', The Cochrane Database of Systematic Reviews, vol. 2, pp. CD007897. https://doi.org/10.1002/14651858.CD007897.pub3

Özbek, M , Bohn, A & Berntsen, D 2015, 'Remembering and Imagining: How does episodic counterfactual thinking fit in?', AIAS conference The Hands of Time: Multiple Perspectives on Aging, 11-12 June 2015., Aarhus, Denmark, 11/06/2015 - 12/06/2015. <http://aias.au.dk/events/the-hands-of-time/>

Özbek, M , Bohn, A & Berntsen, D 2015, 'Remembering and Imagining: How does episodic counterfactual thinking fit in?', SARMAC XI, Victoria, BC, Canada, 24/06/2015 - 27/06/2015. <http://static1.squarespace.com/static/504170d6e4b0b97fe5a59760/t/55887e52e4b0eb99d4b5b905/1435008594892/Program15June.pdf>

Ryzewski, K & McAtackney, L 2015, Remembering ‘home’? Contemporary Irish identity on Montserrat, the ‘Emerald Isle of the Caribbean’ . in Caribbean Irish Connections. . University of West Indies Press, pp. 119-139.

Mortensen, E 2015, Remembering or Concealing Mythical and Historical Events in the Cityscape of Early Roman Ephesus. in E Mortensen & SG Saxkjær (eds), Revealing and Concealing in Antiquity: Textual and Archaeological Approaches to Secrecy. Aarhus Universitetsforlag, Århus, Aarhus Studies in Mediterranean Antiquity, vol. XIII, pp. 53-74.

Tanderup, S 2015, 'Remembering the Book : Nostalgia and Intermediality in Two Contemporary Experimental Novels / Herinneringen aan het Boek : Nostalgie en intermedialiteit in twee hedendaagse experimentele romans', Kunstlicht, vol. 36, no. 1, 9, pp. 115-129.

Le, TH , Madsen, P, Lundeheim, N, Nilsson, K & Norberg, E 2015, REML and Bayesian analysis of association between leg conformation in young pigs and sow longevity. in Book of Abstract of the 66th Annual Meeting of the European Federation of Animal Science. vol. 21, Wageningen Academic Publishers, The Netherlands, EAAP Book of Abstracts, pp. 459-459, 66th Annual Meeting of the European Federation of Animal Science, Warsaw, Poland, 31/08/2015. https://doi.org/10.3920/978-90-8686-816-2

Hess, DC, Blauenfeldt, RA , Andersen, G , Hougaard, KD, Hoda, MN, Ding, Y & Ji, X 2015, 'Remote ischaemic conditioning-a new paradigm of self-protection in the brain', Nature Reviews. Neurology, vol. 11, no. 12, pp. 698-710. https://doi.org/10.1038/nrneurol.2015.223

Krogstrup, NV, Oltean, M, Bibby, BM, Niewenhuijs-Moeke, GJ, Dor, FJ, Birn, H & Jespersen, B 2015, 'Remote Ischaemic conditioning on recipients of deceased renal transplants, effect on immediate and extended kidney graft function: a multicentre, randomised controlled trial protocol (CONTEXT)', BMJ Open, vol. 5, no. 8, e007941. https://doi.org/10.1136/bmjopen-2015-007941

Pickard, JMJ, Bøtker, HE, Crimi, G, Davidson, B, Davidson, SM, Dutka, D, Ferdinandy, P, Ganske, R, Garcia-Dorado, D, Giricz, Z, Gourine, AV, Heusch, G, Kharbanda, R, Kleinbongard, P, MacAllister, R, McIntyre, C, Meybohm, P, Prunier, F, Redington, A, Robertson, NJ, Suleiman, MS, Vanezis, A, Walsh, S, Yellon, DM & Hausenloy, DJ 2015, 'Remote ischemic conditioning: from experimental observation to clinical application: report from the 8th Biennial Hatter Cardiovascular Institute Workshop', Basic Research in Cardiology, vol. 110, no. 1, pp. 453. https://doi.org/10.1007/s00395-014-0453-6

Heusch, G, Bøtker, HE, Przyklenk, K, Redington, A & Yellon, D 2015, 'Remote Ischemic Conditioning', Journal of the American College of Cardiology, vol. 65, no. 2, pp. 177-195. https://doi.org/10.1016/j.jacc.2014.10.031

Birkelund, T , Obad, D, Matejec, R, Bøtker, HE & Ravn, HB 2015, 'Remote ischemic preconditioning does not increase circulating or effector organ concentrations of proopiomelanocortin derivates', Scandinavian Cardiovascular Journal, vol. 49, no. 5, pp. 257-263. https://doi.org/10.3109/14017431.2015.1046401

Bramming Jørgensen, T, Søndergaard, M , Trolle, D, Boye Hansen, L, Kamp Sørensen, M, Closter, RM & Larsen, JE 2015, Remote sensing af søers klorofylindhold: Optimering af vandmiljøovervågning. Naturstyrelsen.

Malinowski, R 2015, 'Remote sensing applications for analysing localised flooding on riverine floodplains'.

Groom, GB , Juel, A & Ejrnæs, R 2015, Remote sensing based classification of structural elements of coastal habitats. Scientific Report from DCE - Danish Centre for Environment and Energy, no. 144.

Bokde, N, Gupta, A, Peshwe, PD & Kulat, K 2015, RemoteWSN: A novel technique for remotely visualizing connectivity in WSN working on a weight based routing algorithm. in National Conference on Recent Advances in Electronics & Computer Engineering. pp. 92-95, Recent Advances in Electronics & Computer Engineering 2015, Roorkee, India, 13/02/2015. <https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=7510232>

Arias, CA, Sanchez, M, Montoya, J & Mendez, A 2015, 'Removal efficiency and clogging in a HSSF industrial wastewater treatment system in Colombia', WETPOL 2015, York, United Kingdom, 13/09/2015 - 19/09/2015.

Lyu, T , Zhang, Y , Carvalho, P , Arias, CA & Brix, H 2015, 'Removal of imazalil and tebuconazole in saturated constructed wetland mesocosms'.

Furgal, KM , Meyer, RL & Bester, K 2015, 'Removing selected steroid hormones, biocides and pharmaceuticals from water by means of biogenic manganese oxide nanoparticles in situ at ppb levels', Chemosphere, vol. 136, pp. 321-326. https://doi.org/10.1016/j.chemosphere.2014.11.059

Mjörnstedt, L, Schwartz Sørensen, S, von Zur Mühlen, B, Jespersen, B, Hansen, JM, Bistrup, C, Andersson, H, Gustafsson, B, Solbu, D & Holdaas, H 2015, 'Renal function three years after early conversion from a calcineurin inhibitor to everolimus: results from a randomized trial in kidney transplantation', Transplant International, vol. 28, no. 1, pp. 42-51. https://doi.org/10.1111/tri.12437

Lauritsen, J, Mortensen, MS, Kier, MGG, Christensen, IJ, Agerbaek, M, Gupta, R & Daugaard, G 2015, 'Renal impairment and late toxicity in germ-cell cancer survivors', Annals of Oncology, vol. 26, no. 1, pp. 173-8. https://doi.org/10.1093/annonc/mdu506

Mathiassen, O , Bech, JN , Buus, NH , Christensen, KL, Vase, H, Bertelsen, J , Rickers, H , Kampmann, U , Pedersen, ME , L. Pedersen, O , Peters, CD , Poulsen, PL , Lassen, JF , Hansen, KW , Bøtker, HE , Schroeder, P, Pedersen, EB & Kaltoft, AK 2015, 'Renal Sympathetic Denervation in Treatment Resistant Essential Hypertension. A Sham-Controlled, Double-blinded Randomized Trial (ReSET trial)', Journal of the American College of Cardiology, vol. 66, no. 15, pp. B41. <http://content.onlinejacc.org/article.aspx?articleid=2452348>

Jensen, TB , Cheema, MU, Szymiczek, A, Damkier, HH & Praetorius, J 2015, 'Renal type a intercalated cells contain albumin in organelles with aldosterone-regulated abundance', PLoS One, vol. 10, no. 4, pp. e0124902. https://doi.org/10.1371/journal.pone.0124902

Bjørnsten , T & Stephensen, JL 2015, 'Renegotiating Data Ecologies through Trees, Soil, and Pigs’ Lungs', Spheres - Journal For Digital Culture, vol. 2. <http://spheres-journal.org/renegotiating-data-ecologies-through-trees-soil-and-pigs-lungs/>

Becker, S, Frew, BA, Andresen, GB, Jacobson, MZ, Schramm, S & Greiner, M 2015, 'Renewable build-up pathways for the US: Generation costs are not system costs', Energy, vol. 81, pp. 437-445. https://doi.org/10.1016/j.energy.2014.12.056

Kerzel, M 2015, Renter. in J Pedersen, M Kerzel, J Ferniss & CH Eriksen (eds), Skatteretten 1. 7 edn, Karnov Group, Kbh., pp. 408-430.

Paldam, M 2015, Rents in a welfare state. in RD Congleton & AL Hillman (eds), Companion to the Political Economy of Rent Seeking. Edward Elgar Publishing, Cheltenham, UK. and Northampton, MA, US, pp. 328-49.

Hajari, JN, Christensen, U, Kiilgaard, JF, Bek, T & la Cour, M 2015, 'Reoperation for rhegmatogenous retinal detachment as quality indicator for disease management: a register study', Acta Ophthalmologica, vol. 93, no. 6, pp. 505-511.

Bak-Nielsen, S , Pedersen, IB , Ivarsen, A & Hjortdal, J 2015, 'Repeatability, reproducibility, and age dependency of dynamic Scheimpflug-based pneumotonometer and its correlation with a dynamic bidirectional pneumotonometry device', Cornea, vol. 34, no. 1, pp. 71-7. https://doi.org/10.1097/ICO.0000000000000293

Søholm, H, Lønborg, J, Andersen, MJ, Vejlstrup, N, Engstrøm, T, Møller, JE & Hassager, C 2015, 'Repeated echocardiography after first ever ST-segment elevation myocardial infarction treated with primary percutaneous coronary intervention--is it necessary?', European Heart Journal: Acute Cardiovascular Care, vol. 4, no. 6, pp. 528-36. https://doi.org/10.1177/2048872614556000

Mannström, P, Kirkegaard, M & Ulfendahl, M 2015, 'Repeated Moderate Noise Exposure in the Rat-an Early Adulthood Noise Exposure Model', J Assoc Res Otolaryngol. https://doi.org/10.1007/s10162-015-0537-5

Peltrea, C, Nyord, T, Bruun, S, Jensen, LS & Magid, J 2015, 'Repeated soil application of organic waste amendments reduces draught force and fuel consumption for soil tillage', Agriculture, Ecosystems & Environment, vol. 211, pp. 94–101. https://doi.org/10.1016/j.agee.2015.06.004

Komoda, Y, Lida, T, Kothari, M, Komiyama, O, Baad-Hansen, L, Kawara , M, Sessle, B & Svensson, P 2015, 'Repeated tongue lift movement induces neuroplasticity in corticomotor control of tongue and jaw muscles in humans', Brain Research, vol. 1627, pp. 70-79. https://doi.org/10.1016/j.brainres.2015.09.016

Løvschal, M & Holst, MK 2015, 'Repeating boundaries: Repertoires of landscape regulations in southern Scandinavia in the Late Bronze Age and Pre-Roman Iron Age', Danish Journal of Archaeology, vol. 3, no. 2, pp. 95-118. https://doi.org/10.1080/21662282.2014.990311

Cho, T-H, Nighoghossian, N, Mikkelsen, IK, Derex, L, Hermier, M, Pedraza, S, Fiehler, J, Østergaard, L, Berthezène, Y & Baron, J-C 2015, 'Reperfusion Within 6 Hours Outperforms Recanalization in Predicting Penumbra Salvage, Lesion Growth, Final Infarct, and Clinical Outcome', Stroke; a journal of cerebral circulation, vol. 46, no. 6, pp. 1582-1589. https://doi.org/10.1161/STROKEAHA.114.007964

He, Q, Cai, Z, Hu, T, Liu, H, Bao, C, Mao, W & Jin, W 2015, 'Repetitive sequence analysis and karyotyping reveals centromere-associated DNA sequences in radish (Raphanus sativus L.)', BMC Plant Biology, vol. 15, no. 105, pp. 1-12. https://doi.org/10.1186/s12870-015-0480-y

Hovmøller, MS , Walter, S, Bayles, R, Amelia Hubbard, A, Flath, K, Sommerfeldt, N, Leconte, M, Rodriguez Algaba, J , Hansen, JG , Lassen, P , Justesen, AF , Ali, S & de Vallavieille-Pope, C 2015, 'Replacement of the European wheat yellow rust population', IPM Innovation in Europe, Poznan, Poland, 14/01/2015 - 16/01/2015.

Soffer, AKB 2015, 'Replacing and representing patients: Professional feelings and plastic body replicas in nursing education', Emotion, Space and Society, vol. 2015, no. 16, pp. 11-18. <http://www.sciencedirect.com.ez.statsbiblioteket.dk:2048/science/journal/17554586/16>

Skovhus, RB , Poulsen, BK, Buhl, R & Thomsen, R 2015, 'Replik om karrierelæring og udsyn i udskolingen', Vejlederen, no. 4, pp. 28-29.

Wendel-Hansen, J 2015, 'Replik til Claus Friisberg: Venstres kamp for folketingsparlamentarisme 1870-1909?', Historisk Tidsskrift, vol. 115, no. 1, pp. 146-152. <https://tidsskrift.dk/historisktidsskrift/article/view/56733/76933 >

Aagaard, K 2015, 'Replik til den norske strukturdebat', Forskningspolitikk, vol. 2015, no. 1, pp. 27. <http://fpol.no/wp-content/uploads/2015/03/Forskningspolitikk-1-2015.pdf>

Sarafoff, N, Byrne, RA, Mehilli, J, Sibbing, D, Kristensen, SD, Laugwitz, K-L, Maeng, M & Kastrati, A 2015, 'Reply: Duration of Triple Therapy in Patients Requiring Oral Anticoagulation After Drug-Eluting Stent Implantation', Journal of the American College of Cardiology, vol. 66, no. 9, pp. 1089-90. https://doi.org/10.1016/j.jacc.2015.06.1312

Sarafoff, N, Byrne, RA, Mehilli, J, Sibbing, D, Kristensen, SD, Laugwitz, K-L, Maeng, M & Kastrati, A 2015, 'Reply: Duration of Triple Therapy: A Clinical Question Yet to Be Answered', Journal of the American College of Cardiology, vol. 66, no. 20, pp. 2266-7. https://doi.org/10.1016/j.jacc.2015.08.886

Kancir, ASP, Ekeløf, NP & Pedersen, EB 2015, 'Reply. Letter to Editor', Anaesthesiology, vol. 123, pp. 483-4.

Kazankov, K , Møller, HJ , Bibby, BM , Vilstrup, H, George, J & Grønbæk, H 2015, 'Reply: Soluble CD163 correlates with the fibrosis models APRI and FIB-4 and independently predicts liver fibrosis in chronic HBV and HCV infection', Hepatology, vol. 61, no. 1, pp. 735-736. https://doi.org/10.1002/hep.27302

Wright, S & Shore, C 2015, 'Reply to comments on “Audit culture revisited: Rankings, ratings, and the reassembling of society"', Current Anthropology, vol. 57, no. 1, pp. 20-25. <http://www.press.uchicago.edu/ucp/journals/journal/ca.html>

Daugaard, G, Kier, MG, Mortensen, MS, Agerbæk, M, Holm, NV, Rørth, M, von der Maase, H, Jarle Christensen, I & Lauritsen, J 2015, 'Reply to C. Rusner et al, L.C. Pagliaro et al, and K. Lu', Journal of Clinical Oncology, vol. 33, no. 20, pp. 2326-7. https://doi.org/10.1200/JCO.2015.60.9313

Displaying results 166501 to 166550 out of 389685

3331

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen