Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Ravn, I, Hvorfor ved så få mennesker, at banker skaber penge? (You Tube titel: "Intro - pengeskabelse"), 2019, Pictures, Video and sound recordings (digital).

Borup, J 2019, Hvor mange buddhister bor der i Danmark?. religion.dk. <https://www.religion.dk/hvor-mange-buddhister-er-der-i-danmark>

Fibiger, MQ 2019, 'Hvor mange hinduer bor i Danmark', Kristeligt Dagblad. <https://k.dk/s/Hs2D>

Gilliam, L 2019, 'Hvornår bliver man dansker?', Hearts & Minds. Magasin om Generationer, pp. 28-29. <https://heartsandminds.fuau.dk/media/10115/hm_temamagasin_2019_mellem.pdf>

Hansen, BB 2019, 'Hvornår er der noget fornuft tilbage i Danmark?', Dagbladet Information.

Kjeldsen, CC 2019, 'Hvornår er godt godt nok, og bør de nationale test stoppes nu?', Folkeskolen, no. 19, pp. 26-27.

Thomsen, KR 2019, 'Hvornår er vi afhængige af noget, og hvornår er et stort brug problematisk?', Generation Skærm, København, Denmark, 12/03/2019 - 12/03/2019.

Østergaard, S , Andersen, S, Sládecek, T & Villesen, P 2019, 'Hvornår får vi børn? – En historie om fødselsmønstre i et moderne samfund', Aktuel Naturvidenskab, vol. 2019, no. 5, pp. 8-11. <https://aktuelnaturvidenskab.dk/find-artikel/nyeste-numre/5-2019/>

Gislum, R 2019, 'Hvor præcist kan vi ramme frøafgrødens kvælstofbehov?', Froeavleren, vol. 102, no. 2, pp. 16-17. <https://froeavleren.e-pages.pub/titles/froeavleren/4732/publications/72>

Larsen, SN 2019, 'Hvor sidder viljen?', Information, pp. 22.

Hermann, A 2019, Hvor to er ét, er helvede forbi: Senromantiske forestillinger om kvinden som mandens forløser. in D Bugge & A Hermann (eds), Moder, søster, elskte: Frelsende kvindeskikkelser i dansk litteratur. Eksistensen Akademisk, København, pp. 11-34.

Ivanov, AS, Mihovska, AD, Poulkov, V & Prasad, R 2019, 'Hybrid Accuracy-Time Trade-off Solution for Spectrum Sensing in Cognitive Radio Networks', International Journal of Mobile Network Design and Innovation, vol. 9, no. 1, pp. 1-13. https://doi.org/10.1504/IJMNDI.2019.098223

Neumayr, T, Saatci, B, Augstein, M, Jetter, H-C, Klokmose, CN, Anderst-Kotsis, G & Rintel, S 2019, Hybrid Collaboration – Moving Beyond Purely Co-Located or Remote Collaboration. in ECSCW 2019: Proceedings of the 17th European Conference on Computer-Supported Cooperative Work: The International Venue on Practice-centred Computing and the Design of Cooperation Technologies - Workshop Proposals. Reports of the European Society for Socially Embedded Technologies: , no. 2, vol. 3. https://doi.org/10.18420/ecscw2019_ws3

Saatci, B , Rädle, R, Rintel, S, O'Hara, K & Klokmose, CN 2019, 'Hybrid Meetings in the Modern Workplace: Stories of Success and Failure', Paper presented at International Conference on Collaboration and Technology, Kyoto, Japan, 03/09/2019 - 06/09/2019 pp. 45-61. https://doi.org/10.1007/978-3-030-28011-6_4

Yang, C & Le, DQS, Hybrid nanoparticle, Patent No. PCT/DK2018/050330. <https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2019110067>

Heinrich, C , Pakkanen, M & Veraart, A 2019, 'Hybrid simulation scheme for volatility modulated moving average fields', Simulation, vol. 166, no. December, pp. 224-244. https://doi.org/10.1016/j.matcom.2019.04.006

Zhang, Y, Gal, N , Itel, F , Westensee, IN , Brodszkij, E, Mayer, D, Stenger, S, Castellote-Borrell, M , Boesen, T, Tabaei, SR, Hook, F & Stadler, B 2019, 'Hybrid vesicles as intracellular reactive oxygen species and nitric oxide generators', Nanoscale, vol. 11, no. 24, pp. 11530-11541. https://doi.org/10.1039/c9nr02584g

Fiandaca, G , Maurya, PK, Balbarini, N, Hördt, A, Møller, I, Rønde, V, Foged, N, Bjerg, PL, Christiansen, AV & Auken, E 2019, Hydraulic permeability prediction from induced polarization data at field scale. in 24th European Meeting of Environmental and Engineering Geophysics. European Association of Geoscientists and Engineers, Houten, The Netherlands, pp. 471-475, 24th European Meeting of Environmental and Engineering Geophysics, Porto, Portugal, 09/09/2018. https://doi.org/10.3997/2214-4609.201802549

Varvaris, I , Kotlar, AM, de Jong van Lier, Q, de Jonge, LW , Iversen, BV & Moldrup, P 2019, 'Hydraulic Properties Estimation by Inverse Modeling of Drip Infiltrometer Experiment Core Ideas', 2018 - 2019 SSSA International Soils Meeting , San Diego, United States, 06/01/2019 - 09/01/2019. https://doi.org/10.13140/RG.2.2.10491.16167

Hawthorne, SB, Miller, DJ, Grabanski, CB, Azzolina, N, Kurz, BA, Ardakani, OH, Smith, SA, Sanei, H & Sorensen, JA 2019, 'Hydrocarbon recovery from williston basin shale and mudrock cores with supercritical co₂: Part 1. Method validation and recoveries from cores collected across the basin', Energy and Fuels, vol. 33, no. 8, pp. 6857-6866. https://doi.org/10.1021/acs.energyfuels.9b01177

Pedersen, SA, Johannesdottir Schmidt, SA, Hölmich, LR, Friis, S, Pottegård, A & Gaist, D 2019, 'Hydrochlorothiazide use and risk for Merkel cell carcinoma and malignant adnexal skin tumors: A nationwide case-control study', Journal of the American Academy of Dermatology, vol. 80, no. 2, pp. 460-465. https://doi.org/10.1016/j.jaad.2018.06.014

Kealy, RA, Busk, T, Goldstein, J, Larsen, PS & Riisgård, HU 2019, 'Hydrodynamic characteristics of aquiferous modules in the demosponge Halichondria panicea', Marine Biology Research, vol. 15, no. 10, pp. 531-540. https://doi.org/10.1080/17451000.2019.1694691

Asadzadeh, SS, Nielsen, LT, Andersen, A, Dölger, J, Kiørboe, T, Larsen, PS & Walther, JH 2019, 'Hydrodynamic functionality of the lorica in choanoflagellates', Journal of the Royal Society Interface, vol. 16, no. 150, 20180478. https://doi.org/10.1098/rsif.2018.0478

Strong, EF, Pezzulla, M, Gallaire, F & Siconolfi, L 2019, 'Hydrodynamic loading of perforated disks in creeping flows', Physical Review Fluids, vol. 4, no. 8, 084101. https://doi.org/10.1103/PhysRevFluids.4.084101

Balog, R , Cassidy, A , Jørgensen, JH , Hansen, LK , Andersen, M , Grubisic Cabo, A, Ravani, F, Bignardi, L, Lacovig, P, Lizzit, S & Hornekær, L 2019, 'Hydrogen interaction with graphene on Ir(1 1 1): a combined intercalation and functionalization study', Journal of Physics: Condensed Matter, vol. 31, no. 8, 085001. https://doi.org/10.1088/1361-648X/aaf76b

Colares, VLP, Lima, SNL, Sousa, NCF, Araújo, MC, Pereira, DMS, Mendes, SJF, Teixeira, SA, Monteiro, CDA, Bandeca, MC, Siqueira, WL, Moffa, EB, Muscará, MN & Fernandes, ES 2019, 'Hydrogen peroxide-based products alter inflammatory and tissue damage-related proteins in the gingival crevicular fluid of healthy volunteers: a randomized trial', Scientific Reports, vol. 9, no. 1, 3457. https://doi.org/10.1038/s41598-019-40006-w

Zlotea, C, Sow, MA, Ek, G, Couzinie, J-P, Perriere, L, Guillot, I, Bourgon, J, Moller, KT , Jensen, TR, Akiba, E & Sahlberg, M 2019, 'Hydrogen sorption in TiZrNbHfTa high entropy alloy', Journal of Alloys and Compounds, vol. 775, pp. 667-674. https://doi.org/10.1016/j.jallcom.2018.10.108

Duque, C, Knee, KL, Russoniello, CJ, Sherif, M, Abu Risha, UA, Sturchio, NC & Michael, HA 2019, 'Hydrogeological processes and near shore spatial variability of radium and radon isotopes for the characterization of submarine groundwater discharge', Journal of Hydrology, vol. 579, 124192. https://doi.org/10.1016/j.jhydrol.2019.124192

Goncalves-Araujo, R, Roettgers, R, Haraguchi, L & Brandini, FP 2019, 'Hydrography-Driven Variability of Optically Active Constituents of Water in the South Brazilian Bight: Biogeochemical Implications', Frontiers in Marine Science, vol. 6, 716. https://doi.org/10.3389/fmars.2019.00716

Christensen, TR , Lund, M , Skov, K, Abermann, J, López-Blanco, E , Scheller, J , Scheel, M , Jackowicz-Korczynski, MA, Langley, K, Murphy, MJ & Mastepanov, M 2019, 'Hydrological intensification causes multiple ecosystem effects in the Arctic', AGU Fall Meeting 2019, Washington, United States, 10/12/2018 - 14/12/2018.

Zhang, Q, Dong, X, Yang, X, Odgaard, BV & Jeppesen, E 2019, 'Hydrologic and anthropogenic influences on aquatic macrophyte development in a large, shallow lake in China', Freshwater Biology, vol. 64, no. 4, pp. 799-812. https://doi.org/10.1111/fwb.13263

Manos, DP & Xydis, G 2019, 'Hydroponics: Are we moving towards that direction only because of the environment? a discussion on forecasting and a systems review', Environmental Science and Pollution Research, vol. 26, no. 13, pp. 12662-12672. https://doi.org/10.1007/s11356-019-04933-5

Nielsen, S, Olsen, MH, Kongsfelt, M, Pedersen, SU & Daasbjerg, K 2019, 'Hydrosilane-Modified Poly(2-Hydroxyethyl Methacrylate) Brush as a Nanoadhesive for Efficient Silicone Bonding', ACS Omega, vol. 4, no. 7, pp. 12130-12135. https://doi.org/10.1021/acsomega.9b01282

Toor, S, Conti, F, Shah, AA, Seehar, TH & Rosendahl, L 2019, Hydrothermal Liquefaction: A Sustainable Solution to Sewage Sludge Disposal Problem. in RP Singh, V Prasad & B Vaish (eds), Advances in Waste-to-Energy Technologies. 1 edn, CRC Press, pp. 143-163. https://doi.org/10.1201/9780429423376-9

Shah, AA, Toor, S, Conti, F & Rosendahl, L 2019, 'Hydrothermal liquefaction of high ash containing sewage sludge at sub and supercritical conditions', 27th European Biomass Conference and Exhibition (EUBCE 2019), Lisbon, Portugal, 27/05/2019 - 30/05/2019.

Larsen, P 2019, 'Hyldestskrift til det moderne bureaukrati', Dagbladet Information.

Bliksted, VF, Frith, CD, Videbech, PB, Fagerlund, B, Emborg, C , Simonsen, A , Roepstorff, A & Campbell-Meiklejohn, D 2019, 'Hyper- and Hypomentalizing in Patients with First-Episode Schizophrenia: fMRI and Behavioral Studies', Schizophrenia Bulletin, vol. 45, no. 2, pp. 377-385. https://doi.org/10.1093/schbul/sby027

Steffensen, C , Dekkers, OM , Lyhne, J , Pedersen, BG , Rasmussen, F , Rungby, J , Poulsen, PL & Jørgensen, JOL 2019, 'Hypercortisolism in Newly Diagnosed Type 2 Diabetes: A Prospective Study of 384 Newly Diagnosed Patients', Hormone and Metabolic Research, vol. 51, no. 1, pp. 62-68. https://doi.org/10.1055/a-0809-3647

Reimer, LCU , Jacobsen, JS & Mechlenburg, I 2019, 'Hypermobility among patients with greater trochanteric pain syndrome', Danish Medical Journal, vol. 66, no. 4, A5539.

Fragniere, AMC, Stott, SRW, Fazal, SV, Andreasen, M, Scott, K & Barker, RA 2019, 'Hyperosmotic stress induces cell-dependent aggregation of α-synuclein', Scientific Reports, vol. 9, 2288. https://doi.org/10.1038/s41598-018-38296-7

Tougaard, RS , Szocska Hansen, ES , Laustsen, C , Nørlinger, TS , Mikkelsen, E , Lindhardt, J , Nielsen, PM , Bertelsen, LB , Schroeder, M , Bøtker, HE , Kim, WY , Wiggers, H & Stødkilde-Jørgensen, H 2019, 'Hyperpolarized [1-13 C]pyruvate MRI can image the metabolic shift in cardiac metabolism between the fasted and fed state in a porcine model', Magnetic Resonance in Medicine, vol. 81, no. 4, pp. 2655-2665. https://doi.org/10.1002/mrm.27560

Bøgh, N , Søvsø Szocska Hansen, E , Christensen, C , Lindhardt, J , Nielsen, PM , Stephenson, RS , Laustsen, C , Hjortdal, VE & Agger, P 2019, 'Hyperpolarized [1-13C]pyruvate spectroscopy assessment of the metabolic reserve in a porcine model of compensated cardiac overload'. <https://www.ismrm.org/19/program_files/O41.htm>

Kurhanewicz, J, Vigneron, DB, Ardenkjaer-Larsen, JH, Bankson, JA, Brindle, K, Cunningham, CH, Gallagher, FA, Keshari, KR, Kjaer, A, Laustsen, C, Mankoff, DA, Merritt, ME, Nelson, SJ, Pauly, JM, Lee, P, Ronen, S, Tyler, DJ, Rajan, SS, Spielman, DM, Wald, L, Zhang, X, Malloy, CR & Rizi, R 2019, 'Hyperpolarized 13C MRI: Path to Clinical Translation in Oncology: Path to Clinical Translation in Oncology', NeoPlasia, vol. 21, no. 1, pp. 1-16. https://doi.org/10.1016/j.neo.2018.09.006

König, A, Schölzel, D, Uluca, B, Viennet, T , Akbey, Ü & Heise, H 2019, 'Hyperpolarized MAS NMR of unfolded and misfolded proteins', Solid State Nuclear Magnetic Resonance, vol. 98, pp. 1-11. https://doi.org/10.1016/j.ssnmr.2018.12.003

Elming, PB , Sørensen, BS, Oei, AL, Franken, NAP, Crezee, J, Overgaard, J & Horsman, MR 2019, 'Hyperthermia: The optimal treatment to overcome radiation resistant hypoxia', Cancers, vol. 11, no. 1, 60. https://doi.org/10.3390/cancers11010060

Mikkelsen, MS, Olesen, B, Kjærgaard, B & Seibæk, L 2019, 'Hyperthermisk intraperitoneal kemoterapi (HIPEC) til kvinder med æggestokkræft: et kirurgisk forskningsprojekt', Fokus på Kræft og Sygepleje, vol. 39, no. 1, pp. 20-24.

Clausen, CT, Hasle, H, Holmqvist, AS, Madanat-Harjuoja, L, Tryggvadottir, L, Wesenberg, F, Bautz, A, Winther, JF & Licht, SDF 2019, 'Hyperthyroidism as a late effect in childhood cancer survivors - an Adult Life after Childhood Cancer in Scandinavia (ALiCCS) study', Acta Oncologica, vol. 58, no. 2, pp. 227-231. https://doi.org/10.1080/0284186X.2018.1535187

Sikjaer, T 2019, Hypoparathyroidism and the skeleton. in Hypoparathyroidism: A Clinical Casebook. Springer International Publishing, pp. 133-141. https://doi.org/10.1007/978-3-030-29433-5_14

Khan, AA, Clarke, B, Rejnmark, L & Brandi, ML 2019, 'Hypoparathyroidism in pregnancy: review and evidence-based recommendations for management', European Journal of Endocrinology. Supplement, vol. 180, no. 2, pp. R37-R44. https://doi.org/10.1530/EJE-18-0541

Jensen, SB & Pedersen, AML 2019, 'Hyposalivation og xerostomi', Aktuel Nordisk Odontologi, vol. 44, no. 1, pp. 58-74. https://doi.org/10.18261/ISSN.2058-7538-2019-01-06

Displaying results 99351 to 99400 out of 390200

1988

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen