Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Ugilt, R , Hansen, BB , Jøker Bjerre, H , Porsgaard, K & Thykjær, S 2015, 'Op i røven med æstetisk politik', Dagbladet Information.

Hansen, CS 2015, Opkomsten af det børnehavepædagogiske vidensfelt i Danmark: Frobel som det modern gennembruds pædagogik, 1870-1905. in S Bayer & JE Kristensen (eds), Kald og kundskab: brydninger i børnehavepædagogikken 1870-2015. U Press, København, pp. 31-78.

Toft, B & Uhrenfeldt, L 2015, 'Oplevelser af at være svært overvægtig og fysisk aktiv: Et kvalitativt systematisk review & meta-syntese', Forsknings og udviklingssymposium, Aalborg, Denmark, 20/11/2015.

Frausing, M, Oplevelsernes herregård - danske herregårde i det 21. århundrede, 2015, Pictures, Video and sound recordings (digital). <http://danmarkshistorien.dk/leksikon-og-kilder/vis/materiale/oplevelsernes-herregaard-danske-herregaarde-i-det-21-aarhundrede/?no_cache=1>

Madsen, L 2015, Opløsning af selskaber og foreninger mv. og overgang i skattepligt. in A Michelsen, S Askholt, J Bolander, J Engsig, IL Jeppesen, AN Laursen & L Madsen (eds), Lærebog om indkomstskat. 16 edn, Djøf Forlag, Kbh., pp. 997-1012.

Skiveren, T 2015, 'Ø-pokalypsen truer', Jyllands-Posten, pp. 26.

Lauridsen, KM & Lillemose, MK (eds) 2015, Opportunities and challenges in the multilingual and multicultural learning space: Final document of the IntlUni Erasmus Academic Network project 2012-15. IntIUni, Aarhus. https://doi.org/10.13140/RG.2.1.1917.9929

Schuwer, R, Gil-Jaurena, I, Hakan Aydin, C, Costello, E, Dalsgaard, C, Brown, M, Jansen, D & Teixeira, A 2015, 'Opportunities and threats of the MOOC movement for higher education: the European perspective', International Review of Research in Open and Distributed Learning, vol. 16, no. 6, pp. 20-38. https://doi.org/10.19173/irrodl.v16i6.2153

Kolstrup, KL 2015, 'Opportunities to Speak. A Qualitative Study of a Second Language in Use', Groningen.

Koffman, JS , Christensen, EA , Marlar, S & Nejsum, LN 2015, 'Opposing effects of cAMP and T259 phosphorylation on plasma membrane diffusion of the water channel aquaporin-5 in Madin-Darby canine kidney cells', PLoS One, vol. 10, no. 7, e0133324, pp. 1-15. https://doi.org/10.1371/journal.pone.0133324

Sørensen, AD 2015, 'Oprør fra livets grænse: Med Albert Camus omkring selvmordets problem', Filosofiske Anmeldelser, vol. 3, no. 3, pp. 76-80. <http://filosofiskselskab.dk/cms/uploads/Filosofiske%20Anmeldelser,Nr3,2015.pdf>

Wendel-Hansen, J 2015, 'Oprør mod københavneriet', Weekendavisen, pp. 12-13.

Rohde, C 2015, Opsigelse. in M Birk, C Rohde & MK Stolt (eds), Lejeloven: med kommentarer. Djøf Forlag, Kbh., pp. 777-825.

Brix, J 2015, 'Opsummerende Læringsrapport om Radikal Innovation i Ikast-Brande kommune', 20 p..

Thorup, O & Laursen, K 2015, 'Optælling af ynglefugle i Vadehavet 2015', 10 p..

Thorup, O & Laursen, K 2015, 'Optællinger af ynglefugle i Vadehavet 2015', 10 p., Notat fra DCE - Nationalt Center for Miljø og Energi (2011-2019).

Nielsen, CF 2015, Optagelse af undervisning - en note. <http://filoffen.dk/optagelse-af-undervisning-en-note/>

Hempel, S, Amon, B, Ammon, C, Hoffmann, G, Berg, W, Menz, C, Zhang, G, Halachmi, I, del Prado, A, Estelles, F, Brunsch, R & Amon, T 2015, OptiBarn – Optimized animal specific barn climatisation facing temperature rise and increased climate variability. in I Körner (ed.), RAMIRAN 2015 : 16th International Conference Rural-Urban Symbiosis . TuTech Verlag , Hamburg, pp. 415-420, 16th International Conference Rural-Urban Symbiosis , Hamburg, Germany, 08/09/2015. https://doi.org/10.15480/882.1297

Mohammadhosseini, H 2015, Optical chips are looking under your skin?. <http://light2015blog.org/2015/08/11/optical-chips-are-looking-under-your-skin/>

Koren, K, Brodersen, KE, Jakobsen, SL & Kühl, M 2015, 'Optical sensor nanoparticles in artificial sediments-a new tool to visualize O₂ dynamics around the rhizome and roots of seagrasses', Environmental Science & Technology, vol. 49, no. 4, pp. 2286-2292. https://doi.org/10.1021/es505734b

Alibrandi, U & Falsone, G 2015, 'Optimal design of dampers in seismic excited structures by the Expected value of the stochastic Dissipated Power', Probabilistic Engineering Mechanics, vol. 41, pp. 129-138. https://doi.org/10.1016/j.probengmech.2015.06.001

Jain, A, Voulis, N, E. Jung, E, F. R. Doud, D, B. Miller, W, Angenent, L & Erickson, D 2015, 'Optimal intensity and biomass density for biofuel production in a thin light-path photobioreactor', Environmental Science & Technology, vol. 49, no. 10, pp. 6327–6334. https://doi.org/10.1021/es5052777

Du, B, Larsen, C & Scheller-Wolf, A 2015, Optimal inventory decisions in a capacitated online retailing system with customer priorities. in Service Systems and Service Management, ICSSSM 2015. 12th International Conference ., 7170335, IEEE, pp. 1-6, 12th International Conference on Service Systems and Service Management, ICSSSM 2015, Guangzhou, China, 22/06/2015. https://doi.org/10.1109/ICSSSM.2015.7170335

Durkota, K, Lisý, W, Kiekintveld, C & Bosansky, B 2015, Optimal Network Security Hardening Using Attack Graph Games. in N Criado, M Rehak, JM Such & L Vercouter (eds), 2nd International Workshop on Agents and Cybersecurity, ACySE 2015: Workshop Notes at AAMAS 2015. Lancaster University, pp. 7-14, International Workshop on Agents and Cybersecurity , Istanbul, Turkey, 05/05/2015. <http://www.lancaster.ac.uk/staff/suchj/acyse2015-proceedings/ACySE2015_proceedings.pdf#page=14>

Toft, S & Albo, MJ 2015, 'Optimal numbers of matings: the conditional balance between benefits and costs of mating for females of a nuptial gift-giving spider.', Journal of Evolutionary Biology, vol. 28, no. 2, pp. 457-467. https://doi.org/10.1111/jeb.12581

Robinius, M, Rodriguez, RA, Kumar, B, Bruun Andresen, G, ter Stein, F, Schiebahn, S & Stolten, D 2015, 'Optimal placement of electrolysers in a German power-to-gas infrastructure', Paper presented at World Hydrogen Energy Conference, Gwangju, Korea, Republic of, 15/06/2014 - 20/06/2014.

Andersen, IT , Hahn, U & Jensen, EBV 2015, 'Optimal PPS Sampling with Vanishing Auxiliary Variables – with Applications in Microscopy', Scandinavian Journal of Statistics, vol. 42, no. 4, pp. 1136–1148. https://doi.org/10.1111/sjos.12156

Agger, P, Lass, T, Smerup, M , Frandsen, J & Pedersen, M 2015, 'Optimal preservation of cardiac tissue prior to DTMRI', Journal of Anatomy, vol. 227, no. 5, pp. 695-701. https://doi.org/10.1111/joa.12377

Ahmadieh Khanesar, M, Kayacan, E & Kaynak, O 2015, Optimal Sliding Mode Type-2 TSK Fuzzy Control of a 2-DOF Helicopter. in Fuzzy Systems (FUZZ-IEEE), 2015 IEEE International Conference on. IEEE, pp. 1-6, 2015 IEEE International Conference on Fuzzy Systems , Istanbul, Turkey, 02/08/2015. https://doi.org/10.1109/FUZZ-IEEE.2015.7337799

Ottosen, C-O 2015, Optimal styring af fugt kan reducere energiforbruget i væksthusgartnerier. in N Halberg (ed.), Perspektiv - Årsberetning 2014 - DCA – Nationalt Center for Fødevarer og Jordbrug. pp. 12-13.

Bektas, T & Lysgaard, J 2015, 'Optimal vehicle routing with lower and upper bounds on route durations', Networks, vol. 65, no. 2, pp. 166-179. https://doi.org/10.1002/net.21592

Kindberg, SF 2015, 'Optimering av smertelindring ved sututering: Transcuta pudendus.'. <http://mm.event123.no/DNJ/Landsmote2015/programfagseminar1.cfm>

Kindberg, S 2015, 'Optimering av smertelindring ved sututering: Transcuta pudendus. Anatomi og praktiske øvelser samt introduksjon av et sertifiseringskonsept for jordmødre'.

Jakob, T, Blank, S & Spillner, E 2015, Optimierte Diagnostik der Insektengiftallergie durch rekombinante Allergene. in J Kleine-Tebbe & T Jakob (eds), Molekulare Allergiediagnostik. Springer, pp. 257-275. https://doi.org/10.1007/978-3-662-45221-9_16

Wahid, R 2015, 'Optimisation of Anaerobic Digestion by Substrates Pre-treatment and Co-digestion'.

Grønbeck, MS , Andersen, BV , Mielby, LH & Byrne, DV 2015, 'Optimisation of Projective Mapping by the use of a panel generated attribute list', Pangborn 2015: "11th Pangborn Sensory Science Symposium", Gothenburg, Sweden, 23/08/2015 - 27/08/2015.

Grønbeck, MS , Andersen, BV , Mielby, LA & Byrne, DV 2015, 'Optimisation of Projective Mapping by the use of a panel generated attribute list', Pangborn 2015: "11th Pangborn Sensory Science Symposium", Gothenburg, Sweden, 23/08/2015 - 27/08/2015.

Mancebo, CM, San Miguel, MÁ, Martínez, MM & Gómez, M 2015, 'Optimisation of rheological properties of gluten-free doughs with HPMC, psyllium and different levels of water', Journal of Cereal Science, vol. 61, pp. 8-15. https://doi.org/10.1016/j.jcs.2014.10.005

Vedelø, TW , Sørensen, JCH & Delmar, C 2015, 'Optimisation of the integrated brain cancer pathway: A study of patient experiences and need for information, care and support', PhD Day, Denmark, 23/01/2015 - 23/01/2015.

Edwards, G , Grøn Sørensen, C , Bochtis, D & Munkholm, LJ 2015, 'Optimised schedules for sequential agricultural operations using a tabu search method', Computers and Electronics in Agriculture, vol. 117, pp. 102-113. https://doi.org/10.1016/j.compag.2015.07.007

Nisbeth Jensen, M 2015, Optimising Comprehensibility in Interlingual Translation: The Need for Intralingual Translation. in K Maksymski, S Gutermuth & S Hansen-Schirra (eds), Translation and Comprehensibility. Frank & Timme, Berlin, TransÜD. Arbeiten zur Theorie und Praxis des Übersetzens und Dolmetschens , vol. 72, pp. 163-194.

Köpke, U, Athmann, M, Han, E & Kautz, T 2015, 'Optimising cropping techniques for nutrient and environmental management in organic agriculture', Sustainable Agriculture Research, vol. 4, no. 3.

Tarp, S 2015, 'Optimismo ciego o ceguera optimista', Estudios de Lexicografía, no. 1, pp. 148-149.

Holm, C 2015, 'Optimistic expectations', ASEMagazine, no. 7, pp. 4-7.

Liu, L, Pang, C, Wu, S & Dong, R 2015, 'Optimization and evaluation of an air-recirculated stripping for ammonia removal from the anaerobic digestate of pig manure', Process Safety and Environmental Protection, vol. 94, no. C, pp. 350-357. https://doi.org/10.1016/j.psep.2014.08.006

Vigna, E & Skibsted, J 2015, Optimization of alkali activated Portland cement – calcined clay blends based on the phase assemblage in the Na₂O-CaO-Al₂O₃-SiO₂-H₂O system. in K Scrivener & A Favier (eds), Calcined Clays for Sustainable Concrete: Proceedings of the 1st International Conference on Calcined Clays for Sustainable Concrete. Springer, RILEM Bookseries, vol. 10, pp. 101-107, 1st International Conference on Calcined Clay for Sustainable Concrete, Zürich, Switzerland, 23/06/2015. https://doi.org/10.1007/978-94-017-9939-3_13

Pauwels, R, Seynaeve, L, Henriques, JCG, de Oliveira-Santos, C, Souza, PC, Westphalen, FH, Rubira-Bullen, IRF, Ribeiro-Rotta, RF, Rockenbach, MIB, Haiter-Neto, F, Pittayapat, P, Bosmans, H, Bogaerts, R & Jacobs, R 2015, 'Optimization of dental CBCT exposures through mAs reduction', Dentomaxillofacial Radiology, vol. 44, no. 9, 20150108. https://doi.org/10.1259/dmfr.20150108

Luca, A , Bach, V & Edelenbos, M 2015, 'Optimization of Headspace Solid-Phase Microextraction and Static Headspace Sampling of Low-Boiling Volatiles Emitted from Wild Rocket (Diplotaxis tenuifolia L.)', Food Analytical Methods, vol. 8, no. 5, pp. 1185-1196. https://doi.org/10.1007/s12161-014-9993-5

Witt, CT , Kronborg, MB, Nohr, EA, Mortensen, PT , Gerdes, C & Nielsen, JC 2015, 'Optimization of heart failure medication after cardiac resynchronization therapy and the impact on long-term survival', European Heart Journal, vol. 1, no. 3, pp. 182-8. https://doi.org/10.1093/ehjcvp/pvv016

Weng, PBS & Johansen, P 2015, 'Optimization of Mechanical Heart Valve Cavitation Detection', BMES 2015 Annual Meeting, Tampa, Florida, United States, 07/10/2015 - 10/10/2015. <http://submissions.mirasmart.com/BMESArchive/Browse.aspx?colID=8>

Displaying results 165151 to 165200 out of 390055

3304