Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Landgrebe, J 2019, Community Mapping. in M Femø Nielsen & S Skriver (eds), Metodekogebogen: 130 analysemetoder fra humaniora og samfundsvidenskab., 10, Upress, København, pp. 420-422.

Hellum, R, Nielsen, AS, Bischof, G, Andersen, K, Hesse, M, Ekstrøm, CT & Bilberg, R 2019, 'Community reinforcement and family training (CRAFT) - design of a cluster randomized controlled trial comparing individual, group and self-help interventions', BMC Public Health, vol. 19, 307. https://doi.org/10.1186/s12889-019-6632-5

Rodríguez-Delgado, A, Fresán, A, Miranda, E, Garza-Villarreal, E, Alcalá-Lozano, R, Duque-Alarcón, X, Balducci, T & Arango de Montis, I 2019, 'Comorbid personality disorders and their impact on severe dissociative experiences in Mexican patients with borderline personality disorder', Nordic Journal of Psychiatry, vol. 73, no. 8, pp. 509-514. https://doi.org/10.1080/08039488.2019.1658127

Shreya, S & Kaushik, BK 2019, 'Compact Modeling of Differential Spin-Orbit Torque based MRAM', 4th IEEE International Conference on Emerging Electronics , Bangalore, India, 17/12/2018 - 19/12/2018. https://doi.org/10.1109/ICEE44586.2018.8937873

Corazza, M, García-Valverde, R, Dam, HF, Hösel, M, Benatto, GADR, Beliatis, MJ, Pastorelli, F, Veenstra, S, Knelissen, G, Galagan, Y, Roesch, R, Faber, T, Hoppe, H, Berson, S, Manceau, M, Chaperon, M, Castro, F, Blakesley, J, Gevorgyan, SA & Madsen, MV 2019, 'Compact multifunctional source-meter system for characterisation of laboratory-scale solar cell devices', Measurement Science and Technology, vol. 30, no. 3, 035901. https://doi.org/10.1088/1361-6501/aafae4

Zhang, Y, Ma, Y, Li, Y, Shen, X, Yu, Y, Pan, Y, Zhang, Y, Zheng, D, Zhao, Y, Ye, T, Li, B, Hu, H, Sun, Y, Zhang, Y, Xiang, J & Chen, H 2019, 'Comparative analysis of co-occurring mutations of specific tumor suppressor genes in lung adenocarcinoma between Asian and Caucasian populations', Journal of Cancer Research and Clinical Oncology, vol. 145, no. 3, pp. 747-757. https://doi.org/10.1007/s00432-018-02828-5

Rajan, MR, Sotak, M, Barrenäs, F, Shen, T, Borkowski, K, Ashton, NJ, Biörserud, C, Lindahl, TL, Ramström, S, Schöll, M, Lindahl, P, Fiehn, O, Newman, JW, Perkins, R, Wallenius, V, Lange, S & Börgeson, E 2019, 'Comparative analysis of obesity-related cardiometabolic and renal biomarkers in human plasma and serum', Scientific Reports, vol. 9, no. 1, 15385. https://doi.org/10.1038/s41598-019-51673-0

Mahalle, N, Bhide, V, Greibe, E , Heegaard, CW , Nexø, E , Fedosov, S & Naik, S 2019, 'Comparative Bioavailability of Synthetic B12 and Dietary Vitamin B12 Present in Cow and Buffalo Milk', Nutrients, vol. 11, no. 2, 304. https://doi.org/10.3390/nu11020304

Andersen, DDE 2019, 'Comparative democratization and democratic backsliding: the case for a historical-institutional approach', Comparative Politics, vol. 51, no. 4, pp. 645-663. <https://www-ingentaconnect-com.ez.statsbiblioteket.dk:12048/contentone/cuny/cp/2019/00000051/00000004/art00009>

Grøn, KL, Glintborg, B, Nørgaard, M , Mehnert, F, Østergaard, M, Dreyer, L, Krogh, NS, Bjørner, JB & Hetland, ML 2019, 'Comparative Effectiveness of Certolizumab Pegol, Abatacept, and Biosimilar Infliximab in Patients With Rheumatoid Arthritis Treated in Routine Care: Observational Data From the Danish DANBIO Registry Emulating a Randomized Trial', Arthritis and Rheumatology, vol. 71, no. 12, pp. 1997-2004. https://doi.org/10.1002/art.41031

Sharma, M, Nazareth, I & Petersen, I 2019, 'Comparative effectiveness of sitagliptin vs sulphonylureas in older people', Age and Ageing, vol. 48, no. 5, pp. 725-732. https://doi.org/10.1093/ageing/afz078

Buron, MD, Chalmer, TA, Sellebjerg, F, Frederiksen, J, Góra, MK, Illes, Z, Kant, M, Mezei, Z, Petersen, T , Rasmussen, PV, Roshanisefat, H, Hassanpour-Kalam-Roudy, H, Sejbæk, T, Tsakiri, A, Weglewski, A, Sorensen, PS & Magyari, M 2019, 'Comparative effectiveness of teriflunomide and dimethyl fumarate: A nationwide cohort study', Neurology, vol. 92, no. 16, pp. e1811-e1820. https://doi.org/10.1212/WNL.0000000000007314

Sun, J, Yu, L, Cai, Z, Zhang, A, Jin, W, Han, Y & Li, Z 2019, 'Comparative karyotype analysis among six Ipomoea species based on two newly identified repetitive sequences', Genome, vol. 62, no. 4, pp. 243-252. https://doi.org/10.1139/gen-2018-0169

Anwar, MZ, Lanzen, A, Bang-Andreasen, T & Jacobsen, CS, Comparative Metatranscriptomics Workflow (CoMW), 2019, Computer programme, Codeocean. https://doi.org/10.24433/CO.1793842.v1

Wilson, P, Linge, H, Matthews, JA, Mourne, RW & Olsen, J 2019, 'Comparative numerical surface exposure-age dating (¹⁰Be and Schmidt hammer) of an early-Holocene rock avalanche at Alstadfjellet, Valldalen, southern Norway', Geografiska Annaler, Series A: Physical Geography, vol. 101, no. 4, pp. 293-309. https://doi.org/10.1080/04353676.2019.1644815

Stergiadis, S, Nørskov, NP , Purup, S, Givens, I & Lee, MRF 2019, 'Comparative Nutrient Profiling of Retail Goat and Cow Milk', Nutrients, vol. 11, no. 10, 2282. https://doi.org/10.3390/nu11102282

Moller, AP, Xia, C, Zhou, B, Che, X, Chu, X, Feng, C, Laursen, K, Morelli, F, Li, W, Liu, J, Quan, Q, Zhang, M, Zhang, Q, Zhan, Q, Ma, L, Wang, H, Zou, F & Liang, W 2019, 'Comparative urbanization of birds in China and Europe based on birds associated with trees', Current Zoology, vol. 65, no. 6, pp. 617-625. https://doi.org/10.1093/cz/zoz007

Burau, V & Blank, RH 2019, Comparing health policy: an assessment of typologies of health systems. in I Geva-May, J Muhleisen & BG Peters (eds), Classics of comparative policy analysis. Routledge.

He, C, Kang, J, Teräsvirta, T & Zhang, S 2019 'Comparing long monthly Chinese and selected European temperature series using the Vector Seasonal Shifting Mean and Covariance Autoregressive model' CREATES Research Paper, no. 2019-19, Institut for Økonomi, Aarhus Universitet, Aarhus, pp. 1-34.

Pedersen, P , Nielsen, CV, Hovgaard Andersen, M, Langagergaard, V, Boes, A, Jensen, OK, Jensen, C, Sørensen, VN & Labriola, M 2019, 'Comparing multidisciplinary and brief intervention in employees with different job relations on sick leave due to low back pain - one year follow-up', Work Disability Prevention & Integration Conference 2019, Odense, Denmark, 04/06/2019 - 07/06/2019.

Secher, AE, Glintborg, B, Gudbergsen, H, Krogh, NS, Sørensen, IJ, Jensen, DV, Christensen, R, Skougaard, M, Pedersen, PL & Hetland, ML 2019, 'Comparing patient-reported outcomes entered at home versus at hospital, and testing touch screens for initial recruitment to scientific trials in arthritis patients', Scandinavian Journal of Rheumatology, vol. 48, no. 3, pp. 178-184. https://doi.org/10.1080/03009742.2018.1522666

Ray, S, Cekanaviciute, E, Lima, IP, Sørensen, BS & Costes, SV 2019, 'Comparing Photon and Charged Particle Therapy Using DNA Damage Biomarkers', International Journal of Particle Therapy, vol. 5, no. 1, pp. 15-24. https://doi.org/10.14338/IJPT-18-00018.1

Kruse, R, Leschinski, C & Will, M 2019, 'Comparing Predictive Accuracy under Long Memory, With an Application to Volatility Forecasting', Journal of Financial Econometrics, vol. 17, no. 2, pp. 180-228. https://doi.org/10.1093/jjfinec/nby011

Wu, W, Dai, RT & Bendixen, E 2019, 'Comparing SRM and SWATH Methods for Quantitation of Bovine Muscle Proteomes', Journal of Agricultural and Food Chemistry, vol. 67, no. 5, pp. 1608-1618. https://doi.org/10.1021/acs.jafc.8b05459

Liu, W, Park, S, Bailey, RT, Molina Navarro, E , Andersen, HE , Thodsen, H, Nielsen, A, Jeppesen, E, Jensen, JS, Jensen, JB & Trolle, D 2019, 'Comparing SWAT with SWAT-MODFLOW hydrological simulations when assessing the impacts of groundwater abstractions for irrigation and drinking water', Hydrology and Earth System Sciences Discussions. https://doi.org/10.5194/hess-2019-232

Bertelsen, KP 2019 'Comparing Tests for Identification of Bubbles' CREATES Research Paper, no. 2019-16, Institut for Økonomi, Aarhus Universitet, Aarhus, pp. 1-74.

Smedley, RK, Buylaert, JP & Újvári, G 2019, 'Comparing the accuracy and precision of luminescence ages for partially-bleached sediments using single grains of K-feldspar and quartz', Quaternary Geochronology, vol. 53, 101007. https://doi.org/10.1016/j.quageo.2019.101007

Kellermann, V, Chown, SL, Schou, MF, Aitkenhead, I, Janion-Scheepers, C, Clemson, A, Scott, MT & Sgrò, CM 2019, 'Comparing thermal performance curves across traits: How consistent are they?', Journal of Experimental Biology, vol. 222, no. 11, jeb193433. https://doi.org/10.1242/jeb.193433

Pittaki-Chrysodonta, Z , Arthur, E, Moldrup, P, Knadel, M , Norgaard, T , Iversen, B & de Jonge, LW 2019, 'Comparing Visible-Near-Infrared Spectroscopy and a Pedotransfer Function for Predicting the Dry Region of the Soil-Water Retention Curve', Vadose Zone Journal, vol. 18, no. 1, 180180. https://doi.org/10.2136/vzj2018.09.0180

Mampentzidis, K 2019, 'Comparison and Construction of Phylogenetic Trees and Networks'.

Molina-Navarro, E, Bailey, RT, Andersen, HE , Thodsen, H , Nielsen, A, Park, S, Jensen, JS, Jensen, JB & Trolle, D 2019, 'Comparison of abstraction scenarios simulated by SWAT and SWAT-MODFLOW', Hydrological Sciences Journal, vol. 64, no. 4, pp. 434-454. https://doi.org/10.1080/02626667.2019.1590583

Rasmussen, MB , Stengaard, C , Sørensen, JT , Riddervold, IS , Søndergaard, HM , Niemann, T , Dodt, KK , Frost, L , Jensen, T , Raungaard, B , Hansen, TM, Giebner, M, Rasmussen, C-H, Bøtker, HE , Kristensen, SD , Maeng, M , Christiansen, EH & Terkelsen, CJ 2019, 'Comparison of Acute Versus Subacute Coronary Angiography in Patients With NON-ST-Elevation Myocardial Infarction (from the NONSTEMI Trial)', The American Journal of Cardiology, vol. 124, no. 6, pp. 825-832. https://doi.org/10.1016/j.amjcard.2019.06.007

Groenestein, CM, Hutchings, NJ, Haenel, HD, Amon, B, Menzi, H, Mikkelsen, MH, Misselbrook, TH, van Bruggen, C, Kupper, T & Webb, J 2019, 'Comparison of ammonia emissions related to nitrogen use efficiency of livestock production in Europe', Journal of Cleaner Production, vol. 211, pp. 1162-1170. https://doi.org/10.1016/j.jclepro.2018.11.143

Gautam, KR , Rong, L , Zhang, G & Abkar, M 2019, 'Comparison of analysis methods for wind-driven cross ventilation through large openings', Building and Environment, vol. 154, pp. 375-388. https://doi.org/10.1016/j.buildenv.2019.02.009

Cronin, IV, Cichota, R & Giltrap, D 2019, 'Comparison of APSIM and DNDC when predicting N2O emissions from urine patches', Climate change, reactive nitrogen, food security, and sustainable agriculture, Garmisch, Germany, 15/04/2019 - 16/04/2019.

Jensen, DB, Larsen, MLV & Pedersen, LJ 2019, 'Comparison of architectures and training strategies for convolutional neural networks intended for location-specific counting of slaughter pigs', Paper presented at The 9th European Conference on Precision Livestock Farming, Cork, Ireland, 26/08/2019 - 29/08/2019 pp. 476-483.

Kottsieper, J, Schückel, U, Schwemmer, P, Fox, AD & Garthe, S 2019, 'Comparison of bivalve communities between moulting and wintering areas used by Common Scoter Melanitta nigra in the German North Sea', Estuarine, Coastal and Shelf Science, vol. 229, 106398. https://doi.org/10.1016/j.ecss.2019.106398

Hageman, JHJ, Danielsen, M, Nieuwenhuizen, AG, Feitsma, AL & Dalsgaard, TK 2019, 'Comparison of bovine milk fat and vegetable fat for infant formula: Implications for infant health', International Dairy Journal, vol. 92, no. May, pp. 37-49. https://doi.org/10.1016/j.idairyj.2019.01.005

Rehman, HU , Knadel, M , de Jonge, LW, Moldrup, P, Greve, MH & Arthur, E 2019, 'Comparison of cation exchange capacity estimated from Vis–NIR spectral reflectance data and a pedotransfer function', Vadose Zone Journal, vol. 18, no. 1, 0192. https://doi.org/10.2136/vzj2018.10.0192

Tozlu, C, Ozenne, B, Cho, TH, Nighoghossian, N, Mikkelsen, IK, Derex, L, Hermier, M, Pedraza, S, Fiehler, J, Østergaard, L , Berthezène, Y, Baron, JC & Maucort-Boulch, D 2019, 'Comparison of classification methods for tissue outcome after ischaemic stroke', European Journal of Neuroscience, vol. 50, no. 10, pp. 3590-3598. https://doi.org/10.1111/ejn.14507

Nielsen, S , Bassler, N, Grzanka, L, Laursen, L, Swakon, J, Olko, P, Andreassen, CN , Alsner, J & Singers Sørensen, B 2019, 'Comparison of Coding Transcriptomes in Fibroblasts Irradiated With Low and High LET Proton Beams and Cobalt-60 Photons', International Journal of Radiation Oncology Biology Physics, vol. 103, no. 5, pp. 1203-1211. https://doi.org/10.1016/j.ijrobp.2018.11.065

Pedersen, S , Zhang, T, Zhou, Y, Aschemann-Witzel, J & Thøgersen, J 2019, 'Comparison of Consumer preferences for Imported Organic Food Products in a Developed and Developing Economy', EMAC 2019, Hamburg, Germany, 28/05/2019 - 31/05/2019.

Grubb, S, Vestergaard, ML, Andersen, AS, Rasmussen, KK, Mamsen, LS, Tuckute, G, Grunnet-Lauridsen, K, Møllgård, K, Ernst, E, Christensen, ST, Calloe, K & Andersen, CY 2019, 'Comparison of cultured human cardiomyocyte clusters obtained from embryos/fetuses or derived from human embryonic stem cells', Stem Cells and Development, vol. 28, no. 9, pp. 608-619. https://doi.org/10.1089/scd.2018.0231

Benhassen, LL , Ropcke, DM , Sharghbin, M , Lading, T , Skov, JK , Tjørnild, MJ , Poulsen, KB , Bechsgaard, T , Skov, SN , Nielsen, SL & Hasenkam, JM 2019, 'Comparison of Dacron ring and suture annuloplasty for aortic valve repair-a porcine study', Annals of Cardiothoracic Surgery, vol. 8, no. 3, pp. 342-350. https://doi.org/10.21037/acs.2019.04.02

Köhler, C, Lübben, J, Krause, L, Hoffmann, C, Herbst-Irmer, R & Stalke, D 2019, 'Comparison of different strategies for modelling hydrogen atoms in charge density analyses', Acta Crystallographica Section B: Structural Science, Crystal Engineering and Materials, vol. 75, pp. 434-441. https://doi.org/10.1107/S2052520619004517

Kwong, YTJ, Apte, SC, Asmund, G, Haywood, MDE & Morello, EB 2019, 'Comparison of Environmental Impacts of Deep-sea Tailings Placement Versus On-land Disposal', Water, Air, and Soil Pollution, vol. 230, no. 12, 287. https://doi.org/10.1007/s11270-019-4336-1

Kristiansen, J , Friborg, MK, Eller, N, Brandt, LPA, Glasscock, DJ, Pihl-Thingvad, J, Persson, R, Beser, A, Asberg, M & Thorsen, SV 2019, 'Comparison of exhaustion symptoms in patients with stress-related and other psychiatric and somatic diagnoses', BMC Psychiatry, vol. 19, no. 1, 84. https://doi.org/10.1186/s12888-019-2066-y

Stougaard, M , Jakobsen, A-K , Johnsen, LØ, Thomsen, R, Holm, K, Franch, O , Knudsen, BR , Riber-Hansen, R , Steiniche, T & Ulhøi, BP 2019, 'Comparison of FISH and NGS Based 1p/19q Co-Deletion in Gliomas'.

Zhao, D, Sheng, B, Wu, Y, Li, H, Xu, D, Nian, Y, Mao, S, Li, C, Xu, X & Zhou, G 2019, 'Comparison of Free and Bound Advanced Glycation End Products in Food: A Review on the Possible Influence on Human Health', Journal of Agricultural and Food Chemistry, vol. 67, no. 51, pp. 14007-14018. https://doi.org/10.1021/acs.jafc.9b05891

Olesen, KKW, Steensig, K, Madsen, M , Thim, T, Jensen, LO, Raungaard, B, Eikelboom, J, Kristensen, SD , Bøtker, HE & Maeng, M 2019, 'Comparison of Frequency of Ischemic Stroke in Patients With Versus Without Coronary Heart Disease and Without Atrial Fibrillation', The American Journal of Cardiology, vol. 123, no. 1, pp. 153-158. https://doi.org/10.1016/j.amjcard.2018.09.011

Displaying results 95151 to 95200 out of 390064

1904

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen