Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Pedersen, LG & Tørring, N 2019, 'Finanskrisen har fået en historie', Slagmark: tidsskrift for idéhistorie , vol. 79. <https://www.slagmark.dk/79-finanskrisen-har-fet-en-historie>

Rattan, S 2019, Findes der et rigtigt tidspunkt at dø? in Hvad døden har lært mig om livet: 21 personlige fortællinger. Kristeligt Dagblads Forlag, Kbh., pp. 179-185. <https://shop.k.dk/collections/boger-e-boger/products/hvad-doden-har-laert-mig-om-livet>

Dietrich, J 2019, 'Findes der moralsk realisme i den Hebraiske Bibel? Om at stille moralen op over for Gud', Dansk Teologisk Tidsskrift, vol. 82 , no. 1, pp. 2-21. <https://www.eksistensen.dk/tidsskrifter/dansk-teologisk-tidsskrift/82-argang-2019/dansk-teologisk-tidsskrift-82-1-2.html>

Tahamtani, F & Riber, AB 2019, Finding the best laying hens for organic farms. CORE Organic Cofound. <https://projects.au.dk/coreorganiccofund/news-and-events/show/artikel/finding-the-best-laying-hens-for-organic-farms/>

Kitzmüller, G, Mangset, M, Evju, AS, Angel, S , Aadal, L, Martinsen, R, Bronken, BA, Kvigne, K, Bragstad, LK, Hjelle, EG, Sveen, U & Kirkevold, M 2019, 'Finding the Way Forward: The Lived Experience of People With Stroke After Participation in a Complex Psychosocial Intervention', Qualitative Health Research, vol. 29, no. 12, pp. 1711-1724. https://doi.org/10.1177/1049732319833366

Dodd, JA & Milstreu, G 2019, 'Find of the (last) decade! The first ships discovered on vertical surfaces on Bornholm', Adoranten, pp. 45-53. <https://www.rockartscandinavia.com/find-of-the-last-decade-the-first-ships-discovered-on-vertical-surfaces-on-bornholm-by-james-dodd-gerhard-milstreu-aa149.php>

Haagh, H 2019, 'Fine-grained Access and Secure Computation on Encrypted Data'.

Peyre, G, Balslev, H, Font, X & Tello, JS 2019, 'Fine-Scale Plant Richness Mapping of the Andean Paramo According to Macroclimate', Frontiers in Ecology and Evolution, vol. 7, 377. https://doi.org/10.3389/fevo.2019.00377

Geraldi, NR, Ortega, A, Serrano, O, Macreadie, PI, Lovelock, CE, Krause-Jensen, D, Kennedy, H, Lavery, P, Pace, ML, Kaal, J & Duarte, CM 2019, 'Fingerprinting blue carbon: Rationale and Tools to Determine the Source of Organic Carbon in Marine Depositional Environments', Frontiers in Marine Science, vol. 6, no. May, 263. https://doi.org/10.3389/fmars.2019.00263

Habibi, S, Gholami, H, Fathabadi, A & Jansen, JD 2019, 'Fingerprinting sources of reservoir sediment via two modelling approaches', Science of the Total Environment, vol. 663, no. May, pp. 78-96. https://doi.org/10.1016/j.scitotenv.2019.01.327

Amador, S, Juul, MØØ, Friis, T & Brincker, R 2019, Finite element model updating using the local correspondence principle. in M Mains & BJ Dilworth (eds), Topics in Modal Analysis and Testing, Volume 9 - Proceedings of the 36th IMAC, A Conference and Exposition on Structural Dynamics 2018: Proceedings of the 36th IMAC, A Conference and Exposition on Structural Dynamics 2018. vol. 9, Springer, Conference Proceedings of the Society for Experimental Mechanics Series, no. 9, pp. 309-314, 36th Conference and Exposition on Structural Dynamics, Orlando, Florida, United States, 12/02/2018. https://doi.org/10.1007/978-3-319-74700-2_34

Svejvig, P 2019, 'Fire år med Project Half Double – Blev de store ambitioner indfriet?', Projektledelse, vol. 23, no. 2, pp. 29-31.

Jacobs, E, Duffy, M, Magolan, J, Galletti Vernazzani, B, Cabrera, E, Landea, R, Buchan, S & Sayigh, L 2019, 'First acoustic recordings of critically endangered eastern South Pacific southern right whales (Eubalaena australis)', Marine Mammal Science, vol. 35, no. 1, pp. 284-289. https://doi.org/10.1111/mms.12519

Knorst, V, Yates, S, Byrne, S, Asp, T, Widmer, F, Studer, B & Kölliker, R 2019, 'First assembly of the gene-space of Lolium multiflorum and comparison to other Poaceae genomes', Grassland Science, vol. 65, no. 2, pp. 125-134. https://doi.org/10.1111/grs.12225

Skouboe, S , Ravkilde, T , Bertholet, J , Hansen, R , Worm, ES , Muurholm, CG , Weber, B , Høyer, M & Poulsen, PR 2019, 'First clinical real-time motion-including tumor dose reconstruction during radiotherapy delivery', Radiotherapy and Oncology, vol. 139, pp. 66-71. https://doi.org/10.1016/j.radonc.2019.07.007

Creevey, O, Grundahl, F, Thevenin, F, Corsaro, E, Palle, PL, Salabert, D, Pichon, B, Collet, R, Bigot, L, Antoci, V & Andersen, MF 2019, 'First detection of oscillations in the Halo giant HD 122563: Validation of seismic scaling relations and new parameters', Astronomy & Astrophysics (A&A), vol. 625, 33. https://doi.org/10.1051/0004-6361/201834721

von Essen, C, Stefansson, G, Mallonn, M, Pursimo, T, Djupvik, AA, Mahadevan, S, Kjeldsen, H, Freudenthal, J & Dreizler, S 2019, 'First light of engineered diffusers at the Nordic Optical Telescope reveal time variability of the optical eclipse depth of WASP-12b', Astronomy & Astrophysics (A&A), vol. 628, 115. https://doi.org/10.1051/0004-6361/201935312

Dudani, S, Graham, J, Wells, JC, Bakouny, Z, Pal, SK, Dizman, N, Donskov, F, Porta, C, de Velasco, G, Hansen, A, Iafolla, M, Beuselinck, B, Vaishampayan, UN, Wood, LA, Liow, E, Yan, F, Yuasa, T, Bjarnason, GA, Choueiri, TK & Heng, DYC 2019, 'First-line Immuno-Oncology Combination Therapies in Metastatic Renal-cell Carcinoma: Results from the International Metastatic Renal-cell Carcinoma Database Consortium', European Urology, vol. 76, no. 6, pp. 861-867. https://doi.org/10.1016/j.eururo.2019.07.048

Larridon, I, Tanaka, N, Liang, Y, Phillips, SM, Barfod, AS, Cho, SH, Gale, SW, Jobson, RW, Kim, YD, Li, J, Muasya, AM, Parnell, JAN, Prajaksood, A, Shutoh, K, Souladeth, P, Tagane, S, Tanaka, N, Yano, O, Mesterházy, A, Newman, MF & Ito, Y 2019, 'First molecular phylogenetic insights into the evolution of Eriocaulon (Eriocaulaceae, Poales)', Journal of Plant Research, vol. 132, no. 5, pp. 589-600. https://doi.org/10.1007/s10265-019-01129-3

Bruix, A, Margraf, JT, Andersen, M & Reuter, K 2019, 'First-principles-based multiscale modelling of heterogeneous catalysis', Nature Catalysis, vol. 2, no. 8, pp. 659-670. https://doi.org/10.1038/s41929-019-0298-3

Hideo-Kajita, A, Garcia-Garcia, HM, Haude, M, Joner, M, Koolen, J, Ince, H, Abizaid, A, Toelg, R, Lemos, PA, von Birgelen, C, Christiansen, EH, Wijns, W, Neumann, FJ, Kaiser, C, Eeckhout, E, Teik, LS, Escaned, J, Azizi, V, Kuku, KO, Ozaki, Y, Dan, K & Waksman, R 2019, 'First Report of Edge Vascular Response at 12 Months of Magmaris, A Second-Generation Drug-Eluting Resorbable Magnesium Scaffold, Assessed by Grayscale Intravascular Ultrasound, Virtual Histology, and Optical Coherence Tomography: A Biosolve-II Trial Sub-Study', Cardiovascular Revascularization Medicine, vol. 20, no. 5, pp. 392-398. https://doi.org/10.1016/j.carrev.2019.02.019

Uslu, B & Gümüs, S 2019, 'First-year Success of Student Teachers: Interrelations with Self-efficacy and Social Intelligence', International Higher education conference, Çanakkale, Turkey, 10/10/2019.

McNulty, YM, Lauring, J , Jonasson, C & Selmer, J 2019, 'Fit-dependent Expatriate Crisis Events: Triggers, Causes and Outcomes', Paper presented at EURAM, Lissabon, 26/06/2019 - 28/06/2019.

Vigh-Larsen, JF , Beck, JH , Daasbjerg, A , Knudsen, CB, Kvorning, T, Overgaard, K , Andersen, TB & Mohr, M 2019, 'Fitness Characteristics of Elite and Subelite Male Ice Hockey Players: A Cross-Sectional Study', Journal of Strength and Conditioning Research, vol. 33, no. 9, pp. 2352-2360. https://doi.org/10.1519/JSC.0000000000003285

Anciaux, YE 2019, 'Fitness Lanscape properties across environments: How to infer Fisher's Geometric Model properties from empirical fitness landscapes across environements ', Nordita meeting, Stockholm, Sweden, 08/07/2019 - 20/12/2019.

Andersen, T 2019, 'Five half truths about IHRM – Data from Scandinavian MNEs', Paper presented at Global IHRM conference, United States, 10/05/2019 - 11/05/2019.

Spaniol, M , Bidmon, CM , Holm, AB & Rohrbeck, R 2019, 'Five strategic foresight tools to enhance business model innovation teaching', Journal of Business Models, vol. 7, no. 3, pp. 77-88. https://doi.org/10.5278/ojs.jbm.v7i3.2637

Sollid, SJM, Dieckman, P, Aase, K, Søreide, E, Ringsted, C & Østergaard, D 2019, 'Five topics health care simulation can address to improve patient safety: results from a consensus process', Journal of Patient Safety, vol. 15, no. 2, pp. 111-120. https://doi.org/10.1097/PTS.0000000000000254

Kirk, M , Rasmussen, KW , Overgaard, SB & Berntsen, D 2019, 'Five weeks of immersive reminiscence therapy improves autobiographical memory in Alzheimer's disease', Memory, vol. 27, no. 4, pp. 441-454. https://doi.org/10.1080/09658211.2018.1515960

Brun, S, Cleemann, LH, Holm, K, Salskov, G, Erlandsen, M , Berglund, A, Andersen, NH & Gravholt, CH 2019, 'Five-Year Randomized Study Demonstrates Blood Pressure Increases in Young Women With Turner Syndrome Regardless of Estradiol Dose', Hypertension, vol. 73, no. 1, pp. 242-248. https://doi.org/10.1161/HYPERTENSIONAHA.118.11742

Muru, K, Reinson, K, Künnapas, K, Lilleväli, H, Nochi, Z , Mosegaard, S, Pajusalu, S, Olsen, RKJ & Õunap, K 2019, 'FLAD1-associated multiple acyl-CoA dehydrogenase deficiency identified by newborn screening', Molecular Genetics and Genomic Medicine, vol. 7, no. 9, e915. https://doi.org/10.1002/mgg3.915

Grønbæk, M, Tybirk, E, Neugart, S, Sundekilde, UK, Schreiner, M & Kristensen, HL 2019, 'Flavonoid Glycosides and Hydroxycinnamic Acid Derivatives in Baby Leaf Rapeseed From White and Yellow Flowering Cultivars With Repeated Harvest in a 2-Years Field Study', Frontiers in Plant Science, vol. 10, 355. https://doi.org/10.3389/fpls.2019.00355

Bondonno, NP, Dalgaard, F, Kyrø, C, Murray, K, Bondonno, CP, Lewis, JR, Croft, KD, Gislason, G, Scalbert, A, Cassidy, A, Tjønneland, A, Overvad, K & Hodgson, JM 2019, 'Flavonoid intake is associated with lower mortality in the Danish Diet Cancer and Health Cohort', Nature Communications, vol. 10, no. 1, 3651. https://doi.org/10.1038/s41467-019-11622-x

Kühle, L & Pedersen, JH 2019, Flere domme.

Bjerre, J 2019, 'Flere læreruddannelser medfører ikke X-factor-tilstande: Replik', Altinget. <https://www.altinget.dk/uddannelse/artikel/dpu-lektor-flere-laereruddannelser-medfoerer-ikke-x-factor-tilstande>

Audrino, F, Huang, C & Okhrin, O 2019, 'Flexible HAR Model for Realized Volatility', Studies in Nonlinear Dynamics & Econometrics, vol. 23, no. 3, 20170080. https://doi.org/10.1515/snde-2017-0080

Fatouhi, F, Hudert, MM, Kayo, G & Kotnik, T 2019, Flex Skin: Developing a material system based on interlocking wooden panels. in PJS Cruz (ed.), Structures and Architecture: Bridging the Gap and Crossing Borders: Proceedings of the Fourth International Conference on Structures and Architecture (ICSA 2019). CRC Press, pp. 730-737, International Conference on Structures and Architecture (ICSA 2019), Lisbon, Portugal, 24/07/2019. https://doi.org/10.1201/9781315229126

Bugnet, L, García, RA, Mathur, S, Davies, GR , Hall, OJ , Lund, MN & Rendle, BM 2019, 'FliPer _Class: In search of solar-like pulsators among TESS targets', Astronomy and Astrophysics, vol. 624, A79. https://doi.org/10.1051/0004-6361/201834780

Konnerup, D & Pedersen, O 2019, Flooding tolerance in wetland plants - the effects of leaf gas films. in CA Arias, CA Ramírez-Vargas, L Peñacoba-Antona & H Brix (eds), Book of Abstracts8th International Symposium on Wetland Pollutant Dynamics and Control, WETPOL 2019, 17 –21 June, 2019, Aarhus University, Denmark. Aarhus University, Aarhus, pp. 158, WETPOL2019: 8th International Symposium on Wetland Pollutants Dynamics and Control, Aarhus, Denmark, 17/06/2019. <http://wetpol.com/wp-content/uploads/2019/06/Book_of_Abstracts_WETPOL2019.pdf>

Adams, DH, Tremain, DM, Paperno, R & Sonne, C 2019, 'Florida lagoon at risk of ecosystem collapse', Science, vol. 365, no. 6457, pp. 991-992. https://doi.org/10.1126/science.aaz0175

Wu, N , Thodsen, H , Andersen, HE , Tornbjerg, H , Baattrup-Pedersen, A & Riis, T 2019, 'Flow regimes filter species traits of benthic diatom communities and modify the functional features of lowland streams - a nationwide scale study', Science of the Total Environment, vol. 651, no. 1, pp. 357-366. https://doi.org/10.1016/j.scitotenv.2018.09.210

Grumiaux, C , Andersen, MK, Colinet, H & Overgaard, J 2019, 'Fluctuating thermal regime preserves physiological homeostasis and reproductive capacity in Drosophila suzukii', Journal of Insect Physiology, vol. 113, pp. 33-41. https://doi.org/10.1016/j.jinsphys.2019.01.001

Davies, LR , Schou, MF , Kristensen, TN & Loeschcke, V 2019, 'Fluctuations in nutrient composition affect male reproductive output in Drosophila melanogaster', Journal of Insect Physiology, vol. 118, 103940. https://doi.org/10.1016/j.jinsphys.2019.103940

Tarling, MS, Smith, SAF & Scott, JM 2019, 'Fluid overpressure from chemical reactions in serpentinite within the source region of deep episodic tremor', Nature Geoscience, vol. 12, no. 12, pp. 1034-1042. https://doi.org/10.1038/s41561-019-0470-z

Pezzulla, M, Gallaire, F & Reis, P 2019, 'Fluid-Structure Interactions in Bristled Insect Wings', 72nd Annual Meeting of the APS Division of Fluid Dynamics, Seattle, United States, 23/11/2019 - 26/11/2019. <http://meetings.aps.org/Meeting/DFD19/Session/L27.1>

Gudnason, DA 2019, 'Fluorescence Control at the DNA / Polymer Interface'.

Staun Larsen, L , Baelum, V , Richards, A & Nyvad, B 2019, 'Fluoride in Saliva and Oral Mucosa after Brushing with 1,450 or 5,000 ppm Fluoride Toothpaste', Caries Research, vol. 53, no. 6, pp. 675-681. https://doi.org/10.1159/000501264

Hippe, K, Gordijn, T, Hajdas, I, Jansen, J, Christl, M, Vockenhuber, C, Maden, C, Akcar, N & Ivy-Ochs, S 2019, 'Fluvial dynamics and ¹⁴C‐¹⁰Be disequilibrium on the Bolivian Altiplano', Earth Surface Processes and Landforms, vol. 44, no. 3, pp. 766-780. https://doi.org/10.1002/esp.4529

Nutz, A, Kwiecien, O, Breitenbach, SFM, Cai, Y, Della Porta, G, Danisch, J, Kabiri, L & Bodin, S 2019, 'Fluvio‐lacustrine sedimentation in the Agadir‐Tissint Feija (Anti‐Atlas, Morocco): A promising palaeoclimate archive for the last glacial cycle in northwest Africa', Depositional Record, vol. 5, pp. 362-387. https://doi.org/10.1002/dep2.65

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