Aarhus University Seal

Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Bøggild, T & Laustsen, L 2015, 'Voting for Dominance? Intra-Group Contexts and Ethnic Markers Shape Preferences for Facial Dominance in Political Candidates', Paper presented at MPSA 2015 Conference, Chicago, United States, 16/04/2015 - 19/04/2015.
Sovacool, B 2015, Vulnerabilities to Climate Change: Adaptation in the Asia-Pacific Region. in PG Harris & G Lang (eds), Routledge Handbook of Environment and Society in Asia . Routledge, London, pp. 353-366. <http://www.routledge.com/books/details/9780415659857/>
Schleicher, M 2015, Vulnerable Genders, Vulnerable Loves: Genealogies in Jewish Religion. in SV Pfister & K Gardiner (eds), Past and Present: Perspectives on Gender and Love. Inter-Disciplinary Press, Oxford, pp. 25-34. <http://www.inter-disciplinary.net/publishing/product/past-and-present-perspectives-on-gender-and-love>
Hermansen, JE, Dalgaard, T, Knudsen, MT & Andersen, MS 2015, 'Vurdering af grøn biomasse: Forskningsplatform for samfundsmæssige og miljømæssige, økologiske og økonomiske vurderinger', Indvielse af pilotanlæg til hydrotermisk forvæskning (Hydro Thermal Liquefaction – HTL) af biomasse med henblik på at producere bio-o, Foulum, Denmark, 22/04/2015 - 22/04/2015.
Berthelsen, JP 2015, 'Vurdering af muligheder og behov for udsætning af bæver (Castor fiber ) i Omme Å, Borris Skydeterræn 2014', 16 p., Notat fra DCE - Nationalt Center for Miljø og Energi (2011-2019).
Holm, TE, Therkildsen, OR & Laursen, K 2015, 'Vurdering af nye aktivitetstidspunkter på skydeområde Rømø i 2015', No. 13685, 5 p., Feb 02, 2015. Notat fra DCE - Nationalt Center for Miljø og Energi (2011-2019).
Stokkink, I & van Kersbergen, K 2015, Waarom en hoe de Deense senaat werd afgeschaft. in C van Baalen, A Bos, H Goslinga, J Ramakers, H Reiding & J Turpijn (eds), Jaarboek Parlementaire Geschiedenis 2015: De Eerste Kamer. Uitgeverij Boom, Amsterdam, pp. 72-83.
Feys, P, Bibby, BM, Baert, I & Dalgas, U 2015, 'Walking capacity and ability are more impaired in progressive compared to relapsing type of multiple sclerosis', European Journal of Physical and Rehabilitation Medicine, vol. 51, no. 2, pp. 207-210.
Skyggebjerg, AK 2015, 'War in Contemporary Danish Children's Literature', Paper presented at The 22nd biennial congress of IRSCL, Worcester, United Kingdom, 08/08/2015 - 12/08/2015.
Weil, NL, Termaat, MF, Rubinstein, SM, El Moumni, M, Zuidema, WP, Derksen, RJ, Krijnen, P, van Bodegom-Vos, L, Wendt, KW, van Kuijk, C, Rosendaal, FR, Breederveld, RS, Goslings, JC, Schipper, IB & van Tulder, MW 2015, 'WARRIOR-trial - is routine radiography following the 2-week initial follow-up in trauma patients with wrist and ankle fractures necessary: study protocol for a randomized controlled trial', Current Controlled Trials in Cardiovascular Medicine, vol. 16. https://doi.org/10.1186/s13063-015-0600-x
Elvert, J 2015, 'Warum soll es in der Geschichte keine Zufälle geben?', Erwaegen, Wissen, Ethik, vol. 26, no. 4, pp. 484-486.
Frei, KM, Coutu, AN, Smiarowski, K, Harrison, R, Madsen, KK, Arneborg, J, Frei, R, Gudmundsson, G, Sindbæk, SM, Woollett, J, Hartman, S, Hicks, M & McGovern, TH 2015, 'Was it for walrus? Viking Age settlement and medieval walrus ivory trade in Iceland and Greenland', World Archaeology, vol. 47, no. 3, pp. 439-466.
Eaton, M 2015, 'Water, Conflict and Cooperation in the History of Canada-United States Relations', Paper presented at Nordic Association for Canadian Studies (NACS) XI Triannual Conference (A Land Shaped by Water: Perspectives on Canada), Turku, Finland, 12/08/2015.
Oh, C & Svendsen, GT 2015, 'Water Management Policy in California: The Status Quo of Command-And-Control', International Journal of Regional Development, vol. 2, no. 2, pp. 61-70. https://doi.org/10.5296/ijrd.v2i2.8405
Krogh, PG, Markussen, T & Bang, AL 2015, Ways of Drifting: 5 Methods of Experimentation in Research through Design. in A Chakrabarti (ed.), ICoRD’15 – Research into Design Across Boundaries Volume 1.: Theory, Research Methodology, Aesthetics, Human Factors and Education.. Springer Publishing Company, pp. 39-50, International conference on research into design, Bangalore, India, 07/01/2015.
Krogh, P, Markussen, T & Bang, AL 2015, 'Ways of Drifting: 5 Methods of Experimentation in Research through Design', Paper presented at International conference on research into design, Bangalore, India, 07/01/2015 - 09/01/2015.
Martin-Nielsen, J 2015, 'Ways of Knowing Climate: Hubert H. Lamb and Climate Research in the UK', Wiley Interdisciplinary Reviews: Climate Change, vol. 6, no. 5, pp. 465-477. https://doi.org/10.1002/wcc.349
Hafner, CM, Laurent, S & Violante, F 2015 'Weak diffusion limits of dynamic conditional correlation models' CREATES Research Paper, no. 2015-03, Institut for Økonomi, Aarhus Universitet, Aarhus.
Birch, TE 2015, Weapons, Metal. in KB Metheny & MC Beaudry (eds), Archaeology of Food: an Encyclopedia. Rowman & Littlefield, Lanham, MD, pp. 538-540.
Fibiger, MQ 2015, '"Weasternization" of the West: Kumbh Mela as a Pilgrimage Place for Spiritual Seekers from the West', Bulletin for the Study of Religion, vol. 44/number 2, no. 10.1558/bsor.v44i2.26351, pp. 15-21. https://doi.org/10.1558/bsor.v44i2.26351
Klokmose, CN, Eagan, JR, Baader, S, Mackay, W & Beaudouin-Lafon, M 2015, Webstrates: Shareable Dynamic Media. in UIST'15 Proceedings of the 28th annual ACM symposium on User interface software and technology (UIST '15). Association for Computing Machinery, ACM User Interface Software and Technology Symposium, Charlotte, United States, 08/11/2015. https://doi.org/10.1145/2807442.2807446
Nichols, V, Verhulst, N, Cox, R & Govaerts, B 2015, 'Weed dynamics and conservation agriculture principles: A review', Field Crops Research, vol. 183, pp. 56-68. https://doi.org/10.1016/j.fcr.2015.07.012
Jabran, K & Chauhan, BS 2015, 'Weed management in aerobic rice systems', Crop Protection, vol. 78, pp. 151-163. https://doi.org/10.1016/j.cropro.2015.09.005