Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Uggerhøj, LE, Poulsen, TJ, Munk, JK, Fredborg, M, Søndergaard, TE, Frimodt-Møller, N, Hansen, PR & Wimmer, R 2015, 'Rational Design of Alpha-Helical Antimicrobial Peptides: Do's and Don'ts', ChemBioChem, vol. 16, no. 2, pp. 242-253. https://doi.org/10.1002/cbic.201402581

Westberg, M , Holmegaard, L , Pimenta, FM , Etzerodt, M & Ogilby, PR 2015, 'Rational design of an efficient, genetically encodable, protein-encased singlet oxygen photosensitizer', Journal of the American Chemical Society, vol. 137, no. 4, pp. 1632-42. https://doi.org/10.1021/ja511940j

Ferapontova, E 2015, 'Rational design of genosensors and aptasensors for cancer diagnosis', SYLICA Workshop on electrochemical analysis of biomolecules and its applications in diagnostics, Brno, Czech Republic, 19/02/2015 - 20/02/2015.

Pontone, G, Patel, MR, Hlatky, MA, Chiswell, K, Andreini, D, Norgaard, BL, Byrne, RA, Curzen, N, Purcell, I, Gutberlet, M, Rioufol, G, Hink, U, Schuchlenz, HW, Feuchtner, G, Gilard, M, de Bruyne, B, Rogers, C & Douglas, PS 2015, 'Rationale and design of the Prospective LongitudinAl Trial of FFRCT: Outcome and Resource IMpacts study', American Heart Journal, vol. 170, no. 3, pp. 438-446.e44. https://doi.org/10.1016/j.ahj.2015.06.002

Bentley-Lewis, R, Aguilar, D, Riddle, MC, Claggett, B, Diaz, R, Dickstein, K, Gerstein, HC, Johnston, P, Køber, LV, Lawson, F, Lewis, EF, Maggioni, AP, McMurray, JJV, Ping, L, Probstfield, JL, Solomon, SD, Tardif, J-C, Wu, Y, Pfeffer, MA, ELIXA Investigators & Rungby, J 2015, 'Rationale, design, and baseline characteristics in Evaluation of LIXisenatide in Acute Coronary Syndrome, a long-term cardiovascular end point trial of lixisenatide versus placebo', American Heart Journal, vol. 169, no. 5, pp. 631-638.e7. https://doi.org/10.1016/j.ahj.2015.02.002

Ferapontova, E 2015, 'Rationalizing design of genosensors exploiting electric properties of DNA', Next Generation Tools for DNA Diagnostics, Edinburgh, United Kingdom, 26/02/2015 - 26/02/2015.

Knight, C & Albertsen, A 2015, 'Rawlsian Justice and Palliative Care', Bioethics, vol. 29, no. 8, pp. 536–542. https://doi.org/10.1111/bioe.12156

Elm, J, Norman, P & Mikkelsen, KV 2015, 'Rayleigh light scattering properties of atmospheric molecular clusters consisting of sulfuric acid and bases', Physical Chemistry Chemical Physics, vol. 17, no. 24, pp. 15701-15709. https://doi.org/10.1039/c5cp01012h

Jan, S, Schwering, A, Schultz, CPL & Chipofya, M 2015, RCC11: A Finer Topological Representation for the Alignment of Regions in Sketch Maps. in 28th International Workshop on Qualitative Reasoning. 28th International Workshop on Qualitative Reasoning, Minneapolis, Minnesota, United States, 10/08/2015. <https://www.researchgate.net/publication/278685943_RCC11_A_Finer_Topological_Representation_for_the_Alignment_of_Regions_in_Sketch_Maps>

Svensson, E , Horváth-Puhó, E , Thomsen, RW , Djurhuus, JC , Pedersen, L , Borghammer, P & Sørensen, HT 2015, 'Re: Protective effect of vagotomy suggests source organ for Parkinson's disease', Annals of Neurology. https://doi.org/10.1002/ana.24500

Andersen, CK & Blais, A 2015, 'Reaching ultrastrong coupling with Josephson junction arrays'.

Grigoras, I, Toor, SS & Rosendahl, LA 2015, 'Reaction mechanisms and kinetics of processing glucose, xylose and glucose-xylose mixtures under hot compressed water conditions for predicting bio-crude composition', 4th International Conference on Thermochemical Conversion Science (TcBiomass 2015), Chicago, IL, United States, 02/11/2015 - 05/11/2015.

Bennett, JM, Marks, NA, Miwa, JA, Lopinski, GP, Rosei, F, McKenzie, DR & Warschkow, O 2015, 'Reaction pathways for pyridine adsorption on silicon (001)', Journal of Physics: Condensed Matter, vol. 27, no. 5, 054001. https://doi.org/10.1088/0953-8984/27/5/054001

Gomez, CRSQ & Skibsted, J 2015, Reactivity of heated kaolinite from a combination of chemical methods and solid-state NMR spectroscopy. in ICCC 2015 Beijing : the 14th international congress on the chemistry of cement, 13-16 October, 2015. pp. 310, 14th International Congress on the Chemistry of Cement, Beijing, China, 13/10/2015.

Gomez, CRSQ & Skibsted, J 2015, Reactivity of heated kaolinite from a combination of solid-state NMR and chemical methods. in K Scrivener & A Favier (eds), Calcined Clays for Sustainable Concrete: Proceedings of the 1st International Conference on Calcined Clays for Sustainable Concrete. Springer, RILEM Bookseries, vol. 10, pp. 125-132, 1st International Conference on Calcined Clay for Sustainable Concrete, Zürich, Switzerland, 23/06/2015. https://doi.org/10.1007/978-94-017-9939-3_16

Albrechtslund, A & Albrechtslund, A-MB 2015, 'Reading, Tracking and Intimacy: The dynamics and implications of a digitized culture', Paper presented at Profile, Predict and Prevent, Paris, France, 30/10/2015 - 31/10/2015.

Olesen, KV 2015, 'Realizing Conditional Distributions and Coherence Across Financial Asset Classes', Århus.

Wahlgren, B & Nistrup, U 2015, 'Realkompetence: hvordan kan kortuddannede få deres praktiske kompetencer anerkendt i en erhvervsuddannelse?', Dansk Pædagogisk Tidsskrift, vol. 63, no. 1, pp. 89-97.

Andersen, NB 2015, 'Real Paley-Wiener theorems and Roe's theorem associated with the Opdam-Cherednik transform', Journal of Mathematical Analysis and Applications, vol. 427, no. 1, pp. 47-59. https://doi.org/10.1016/j.jmaa.2015.02.032

Raetzsch, C 2015, 'Real Pictures of Current Events: The photographic legacy of journalistic objectivity', Media History, vol. 21, no. 3, pp. 294-312. https://doi.org/10.1080/13688804.2015.1053387

Gulløv, E 2015, Real siblings. in I Winther, C Palludan & MM Rehder (eds), Sibling: practical and sensitive relations . Danish School of Education, Aarhus University, Copenhagen, pp. 23-36. <http://ebooks.au.dk/index.php/aul/catalog/book/114>

Maximov, II, Vinding, MS, Tse, DHY, Nielsen, NC & Shah, NJ 2015, 'Real-time 2D spatially selective MRI experiments: Comparative analysis of optimal control design methods', Journal of Magnetic Resonance, vol. 254, no. 110-120, pp. 110-120. https://doi.org/10.1016/j.jmr.2015.03.003

Volosniev, AG, Hammer, H-W & Zinner, NT 2015, 'Real-Time Dynamics of an Impurity in an Ideal Bose Gas in a Trap', Physical Review A, vol. 92, no. 2, 023623. https://doi.org/10.1103/PhysRevA.92.023623

Cox, G 2015, Real-time for Pirate Cinema. Postscriptum, vol. 20, Aksioma, Ljubljana. <http://aksioma.org/Geoff-Cox-Real-time-for-Pirate>

Whiting, C, Shtyrov, Y & Marslen-Wilson, W 2015, 'Real-time functional architecture of visual word recognition', Journal of Cognitive Neuroscience (Online), vol. 27, no. 2, pp. 246-65. https://doi.org/10.1162/jocn_a_00699

Kristoffersen, EL , Jørgensen, LA , Franch, O , Etzerodt, M , Frøhlich, R , Bjergbæk, L , Stougaard, M , Ho, Y-P & Knudsen, BR 2015, 'Real-time investigation of human topoisomerase I reaction kinetics using an optical sensor: a fast method for drug screening and determination of active enzyme concentrations', Nanoscale, vol. 7, no. 21, pp. 9825-9834. https://doi.org/10.1039/c5nr01474c

Zuccaro, L, Tesauro, C, Kurkina, T, Fiorani, P, Ki Yu, H, Knudsen, BR, Kern, K, Desideri, A & Balasubramanian, K 2015, 'Real-Time Label-Free Direct Electronic Monitoring of Topoisomerase Enzyme Binding Kinetics on Graphene', ACS Nano, vol. 9, no. 11, pp. 11166-11176. https://doi.org/10.1021/acsnano.5b05709

Hokland, P & Cotter, F 2015, 'Real world data on acute myeloid leukaemia therapy from the developing world - an eye-opener', British Journal of Haematology, vol. 10.1111, no. 13410, 25855402. https://doi.org/10.1111/bjh.13410

Mariegaard, NVE 2015, 'Reappropriating Sovereignty: A Critique of Giorgio Agamben’s Abandonment of Sovereignty', Tropos, vol. Anno VIII, no. 1, pp. 155-179.

Devriese, D, Piessens, F & Birkedal, L 2015 'Reasoning about Object Capabilities with Logical Relations and Effect Parametricity: Technical Report including Proofs and Details' Association for Computing Machinery. <http://cs.au.dk/~birke/papers/object-capabilities-tr.pdf>

Park, S & Hendry, D 2015, 'Reassessing Schoenfeld Residual Tests of Proportional Hazards in Political Science Event History Analyses', American Journal of Political Science, vol. 59, no. 4, pp. 1072-1087. https://doi.org/10.1111/ajps.12176

Thomsen, JPF & Birkmose, AE 2015, 'Re-assessing Self-Interest Theory: Can Intergroup Contact Constrain Self-Interested Reactions?', International Journal of Comparative Sociology, vol. 56, no. 2, pp. 98-115. https://doi.org/10.1177/0020715215598351

Pasick, J, Berhane, Y, Joseph, T, Bowes, V, Hisanaga, T, Handel, K & Alexandersen, S 2015, 'Reassortant highly pathogenic influenza a H5N2 virus containing gene segments related to eurasian H5N8 in British Columbia, Canada, 2014', Scientific Reports, vol. 5, 9484. https://doi.org/10.1038/srep09484

Arboe, T 2015, Receding idioms in West Danish (Jutlandic). in E Piirainen & A Sherris (eds), Language Endangerment: Disappearing metaphors and shifting conceptualizations. John Benjamins Publishing Company, Amsterdam / Philadelphia, Cognitive Linguistic Studies in Cultural Contexts, vol. 7, pp. 155-174. https://doi.org/10.1075/clscc.7.07arb

Engberg, J 2015, 'Recension af: The Faces of the Other: Religious Rivalry and Ethnic Encounters in the Later Roman World. (Cursor mundi, 109: Maijastina Kahlos (red.), Turnhout: Brepols, 2011', Patristica Nordica Annuaria, vol. 29, pp. 142-146.

Barndorff-Nielsen, OE, Benth, FE & Veraart, A 2015, 'Recent advances in ambit stochastics with a view towards tempo-spatial stochastic volatility/intermittency', Banach Center Publications, vol. 104, pp. 25-60. https://doi.org/10.4064/bc104-0-2

Zhang, N-Z, Wang, M, Xu, Y, Petersen, E & Zhu, X-Q 2015, 'Recent advances in developing vaccines against Toxoplasma gondii: an update', Expert Review of Vaccines, vol. 14, no. 12, pp. 1609-21. https://doi.org/10.1586/14760584.2015.1098539

Abbiati, G, Cazzador, E, Lanese, I, Eftekhar Azam, S, S. Bursi, O & Pavese, A 2015, Recent advances on the hybrid simulation of bridges base on partitioned time integration, dynamic identification and model updating. in 6th International Conference on Advances in Experimental Structural Engineering 11th International Workshop on Advanced Smart Materials and Smart Structures Technology . 6th International Conference on Advances in Experimental Structural Engineering 11th International Workshop on Advanced Smart Materials and Smart Structures Technology , Urbana-Champaign , Illinois, United States, 01/08/2015.

Mirzaei, PA 2015, 'Recent challenges in modeling of urban heat island', Sustainable Cities and Society, vol. 19, 266, pp. 200-206. https://doi.org/10.1016/j.scs.2015.04.001

Fox, AD, Christensen, TK, Dalby, L & Nagy, S 2015, 'Recent changes in wintering Eurasian Wigeon numbers in northwest Europe', Pan-European Duck Symposium, Hanko, Finland, 07/04/2015 - 11/04/2015.

Fleming-Lehtinen, V, Andersen, J , Carstensen, J, Lysiak-Pastuszak, E, Murray, C, Pyhälä, M & Laamanen, M 2015, 'Recent developments in assessment methodology reveal that the Baltic Sea eutrophication problem is expanding', Ecological Indicators, vol. 48, pp. 380-388. https://doi.org/10.1016/j.ecolind.2014.08.022

Lynge, ME , Schattling, P & Stadler, BM 2015, 'Recent developments in poly(dopamine)-based coatings for biomedical applications', Nanomedicine, vol. 10, no. 17, pp. 2725-2742. https://doi.org/10.2217/nnm.15.89

Fox, AD 2015, 'Recent shifts in wintering distributions of common dabbling and diving duck species in northwest Europe', Living on a flyway:habitat processes and bird population dynamics from breeding to wintering grounds, Helsinki, Finland, 12/03/2015 - 12/03/2015.

Trip, AK, Stiekema, J, Visser, O, Dikken, JL, Cats, A, Boot, H, van Sandick, JW, Jansen, EPM & Verheij, M 2015, 'Recent trends and predictors of multimodality treatment for oesophageal, oesophagogastric junction, and gastric cancer: A Dutch cohort-study', Acta oncologica (Stockholm, Sweden), vol. 54, no. 10, pp. 1754-62. https://doi.org/10.3109/0284186X.2015.1009638

Læssøe, J & Mochizuki, Y 2015, 'Recent trends in national policy on education for sustainable development and climate change education', Journal of Education for Sustainable Development, vol. 9, no. 1, pp. 27-43. https://doi.org/10.1177/0973408215569112

Kawaharada, Y , Kelly, S , Nielsen, MW, Hjuler, CT, Gysel, K, Muszyński, A, Carlson, RW, Thygesen, MB , Sandal, N , Asmussen, MH , Vinther, M , Andersen, SU , Krusell, L , Thirup, S , Jensen, KJ, Ronson, CW, Blaise, M , Radutoiu, S & Stougaard, J 2015, 'Receptor-mediated exopolysaccharide perception controls bacterial infection', Nature, vol. 523, pp. 308-312. https://doi.org/10.1038/nature14611

Bjerregaard-Olesen, C & Bonefeld-Jørgensen, EC 2015, 'Receptor research on xenohormone effects of human serum extracts containing the actual mixture of perfluorinated alkyl acids: a short review', Receptors and Clinical Investigation, vol. 2, no. 3, pp. e702. <http://www.smartscitech.com/index.php/rci/article/view/702/pdf_97>

Bjønness, J & Krøll, LT 2015, 'Receptpligtig medicin bruges også uden recept', STOF - Viden om rusmidler og samfund, no. 25, pp. 89.

Povlsen, KK 2015, 'Recipes for Old Age: Women's magazines and blogs 1920-2015', Paper presented at Food and the life-span in Europe 1800-2000, København, Denmark, 28/09/2015 - 30/09/2015.

Lysgaard, JG & Sund, P 2015, 'Reclaim “Education” in environmental and sustainability education research', Paper presented at World Environmental Education Conference, Gothenbrug, Sweden, 29/06/2015 - 02/07/2015.

Displaying results 166301 to 166350 out of 389732

3327

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen