Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Pedersen, MGB , Olsen, MS , Schmidt, M , Johnsen, SP, Learn, C, Laursen, HB & Madsen, NL 2019, 'Ischemic Stroke in Adults With Congenital Heart Disease: A Population-Based Cohort Study', Journal of the American Heart Association, vol. 8, no. 15, 011870. https://doi.org/10.1161/JAHA.118.011870

Wang, QJ & Spence, C 2019, 'Is complexity worth paying for? Investigating the perception of wine complexity for single varietal and blended wines in consumers and experts', Australian Journal of Grape and Wine Research, vol. 25, no. 2, pp. 243-251. https://doi.org/10.1111/ajgw.12382

Aly, A, Bernardos, A, Fernandez-Peuchena, CM, Jensen, SS & Pedersen, AB 2019, 'Is Concentrated Solar Power (CSP) a feasible option for Sub-Saharan Africa?: Investigating the techno-economic feasibility of CSP in Tanzania', Renewable Energy, vol. 135, no. May, pp. 1224-1240. https://doi.org/10.1016/j.renene.2018.09.065

Domic, D, Bertl, K, Ahmad, S, Schropp, L, Hellén-Halme, K & Stavropoulos, A 2019, 'Is detection accuracy of buccal bone level at implants in CBCT dependent on bone thickness?', Clinical Oral Implants Research, vol. 30, no. 19, pp. 6.

Juhl, B, Lauszus, F & Lykkesfeldt, J 2019, 'Is Diabetes Associated with Lower Vitamin C Status in Pregnant Women? A Prospective Study', International Journal for Vitamin and Nutrition Research, vol. 86, no. 5-6, pp. 184-189. https://doi.org/10.1024/0300-9831/a000407

Ekblad, M, Falås, P, El-taliawy, H, Nilsson, F, Bester, K, Hagman, M & Cimbritz, M 2019, 'Is dissolved COD a suitable design parameter for ozone oxidation of organic micropollutants in wastewater?', Science of the Total Environment, vol. 658, pp. 449-456. https://doi.org/10.1016/j.scitotenv.2018.12.085

Ågård, AS 2019, 'Is family-witnessed resuscitation a viable practice in the ICU? Findings from an international literature review', Paper presented at 8th EfCCNa Congress 2019, Ljubljana, Slovenia, 13/02/2019 - 16/02/2019.

Neumann, L 2019, Is formality legitimacy? defining the obligations of non-state armed groups. in B Lemann Kristiansen, K Mitkidis, L Munkholm, L Neumann & C Pelaudeix (eds), Transnationalisation and legal actors: legitimacy in question. Routledge, Abingdon, pp. 216-229.

Liljensøe, A, Laursen, JO, Søballe, K & Mechlenburg, I 2019, 'Is high body mass index a potential risk factor for poor outcome after hip arthroplasty? A cohort study of 98 patients 1 year after surgery', Acta Orthopaedica Belgica, vol. 85, no. 1, pp. 91-99. <http://www.actaorthopaedica.be/archive/volume-85/issue-1/original-studies/high-bmi-potential-risk-factor-poor-outcome-hip-arthroplasty-cohort-study-98-patients-1-year-surgery/>

Kierkegaard, S , Mechlenburg, I , Lund, B , Rømer, L , Søballe, K & Dalgas, U 2019, 'Is hip muscle strength normalised in patients with femoroacetabular impingement syndrome one year after surgery? Results from the HAFAI cohort', Journal of Science and Medicine in Sport, vol. 22, no. 4, pp. 413-419. https://doi.org/10.1016/j.jsams.2018.10.004

Lidegaard, N, Normann, U & Ellegaard, C 2019 'Is it just a feeling? Dimensions of intuitive sourcing and supplier-related decisions in SMEs'.

Christensen, JT , Hansen, EM, Hallama, M, Kandeler, E & Rubæk, GH 2019, 'Is it possible to enhance the utilisation of soil phosphorus in a main crop by adapting species and management of the preceding cover crop.', International Phosphorous Workshop 2019 - (IPW9), Zurich, Switzerland, 08/07/2019 - 12/07/2019 pp. 74.

Nyboe, L , Lemcke, S , Møller, AV & Stubbs, B 2019, 'Is it possible to prevent weight gain in young, newly diagnosed patients with schizophrenia or bipolar disorder? A systematic review of the effectiveness of lifestyle interventions', International congress of European society for child and adolescent psychiatry, Wien, Austria, 30/06/2019 - 02/07/2019.

Torp, NC, Weidle, B, Thomsen, PH, Skarphedinsson, G, Aalberg, M, Nissen, JB, Melin, KH, Dahl, K, Valderhaug, R & Ivarsson, T 2019, 'Is it time to rethink standard dosage of exposure-based cognitive behavioral therapy for pediatric obsessive-compulsive disorder?', Psychiatry Research, vol. 281, 112600. https://doi.org/10.1016/j.psychres.2019.112600

Wejse, C & Hønge, BL 2019, 'Is it time to revise the notion that HIV-2 is benign?', The Lancet HIV, vol. 6, no. 1, pp. e3-e4. https://doi.org/10.1016/S2352-3018(18)30265-0

Lippert-Rasmussen, K 2019, 'Is it unjust that elderly people suffer from poorer health than young people? Distributive and relational egalitarianism on age-based health inequalities', Politics, Philosophy & Economics, vol. 18, no. 2, pp. 145-164. https://doi.org/10.1177/1470594X19828020

Sedgwick, M 2019, Islam. Virginia Commonwealth University. <https://wrldrels.org/2019/06/07/islam-2/>

Meret, S & Gregersen, AB 2019, Islam as a "floating signifier": right-wing populism and perceptions of Muslims in Denmark. in The One Percent Problem: Muslims in the West and the Rise of the New Populists. Brookings Institution Press. <https://www.brookings.edu/research/islam-as-a-floating-signifier-right-wing-populism-and-perceptions-of-muslims-in-denmark/>

Riexinger, MT 2019, Islam, Demokratie und Rechtstaat: Versuch einer Entwirrung. in JE Klußmann, M Kreutz & A Sarhan (eds), Reformation im Islam: Perspektiven und Grenzen. Springer Fachmedien Wiesbaden, Wiesbaden, pp. 61-75, Reformation und Islam – Reformation im Islam?, Düsseldorf, Germany, 07/10/2016.

Sedgwick, M 2019, 'Islamic and Western Esotericism', Correspondences: Journal for the Study of Esotericism, vol. 7, no. 1, pp. 277-299.

Suhr, C 2019, 'Islamic exorcism and the cinema fist: Analyzing exorcism among Danish Muslims through the prism of film', Contemporary Islam, vol. 13, no. 1, pp. 121-137. https://doi.org/10.1007/s11562-017-0394-6

Rognlien, L 2019, Islamisk feminisme. Baggrund. <http://baggrund.com/islamisk-feminisme/>

Tørring, PM, Larsen, MJ, Brasch-Andersen, C, Krogh, LN, Kibæk, M, Laulund, L, Illum, N, Dunkhase-Heinl, U, Wiesener, A, Popp, B, Marangi, G, Hjortshøj, TD, Ek, J, Vogel, I , Becher, N, Roos, L, Zollino, M & Fagerberg, CR 2019, 'Is MED13L-related intellectual disability a recognizable syndrome?', European Journal of Medical Genetics, vol. 62, no. 2, pp. 129-136. https://doi.org/10.1016/j.ejmg.2018.06.014

Pavese, N 2019, 'Is neuromelanin the imaging biomarker for the early diagnosis of Parkinson's disease that we were looking for?', Parkinsonism and Related Disorders, vol. 58, pp. 1-2. https://doi.org/10.1016/j.parkreldis.2018.12.013

Sørensen, H , Nielsen, DB , Jacobsen, JS , Søballe, K & Mechlenburg, I 2019, 'Isokinetic dynamometry and gait analysis reveal different hip joint status in patients with hip dysplasia', Hip International, vol. 29, no. 2, pp. 215-221. https://doi.org/10.1177/1120700018773401

Yan, Y , Chang, CC , Venø, MT , Mothershead, CR , Su, J & Kjems, J 2019, 'Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells', Journal of visualized experiments : JoVE, vol. 2019, no. 145, e58655. https://doi.org/10.3791/58655

Tarín-Carrasco, P, Morales-Suarez-Varela, M, Im, U , Brandt, J, Palacios-Peña, L & Jiménez-Guerrero, P 2019, 'Isolating the climate change impacts on air-pollution-related-pathologies over central and southern Europe: A modelling approach on cases and costs', Atmospheric Chemistry and Physics, vol. 19, no. 14, pp. 9385-9398. https://doi.org/10.5194/acp-19-9385-2019

Moodley, A, Kot, W, Nälgård, S, Jakociune, D, Neve, H, Hansen, LH, Guardabassi, L & Vogensen, FK 2019, 'Isolation and characterization of bacteriophages active against methicillin-resistant Staphylococcus pseudintermedius', Research in Veterinary Science, vol. 122, pp. 81-85. https://doi.org/10.1016/j.rvsc.2018.11.008

Aubid, NN, Liu, Y, Peralvo Vidal, JM & Hall, VJ 2019, 'Isolation and culture of porcine primary fetal progenitors and neurons from the developing dorsal telencephalon', Journal of Veterinary Science, vol. 20, no. 2, e3. https://doi.org/10.4142/jvs.2019.20.e3

Alonso-Herranz, L, Porcuna, J & Ricote, M 2019, 'Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity', Methods in molecular biology (Clifton, N.J.), vol. 1951, pp. 59-73. https://doi.org/10.1007/978-1-4939-9130-3_5

Tsochatzis, ED, Lopes, JA, Holland, MV, Reniero, F, Emons, H & Guillou, C 2019, 'Isolation, characterization and structural elucidation of polybutylene terephthalate cyclic oligomers and purity assessment using a ¹H qNMR method', Polymers, vol. 11, no. 3, 464. https://doi.org/10.3390/polym11030464

Alstrup, T , Eijken, M , Bohn, AB , Møller, B & Damsgaard, TE 2019, 'Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat', Current Protocols in Stem Cell Biology, vol. 48, no. 1, e68. https://doi.org/10.1002/cpsc.68

Rahimi, K , Füchtbauer, AC , Fathi, F, Mowla, SJ & Füchtbauer, E-M 2019, 'Isolation of cancer stem cells by selection for miR-302 expressing cells', PeerJ, vol. 7, e6635. https://doi.org/10.7717/peerj.6635

Bhattarai, B, Steffensen, SK & Fomsgaard, IS 2019, 'Isolation of new benzoxazinoids', Copenhagen Bioscience Conferences-Natural Products Discovery, Biosynthesis and Application, Copenhagen, Denmark, 05/05/2019 - 09/05/2019 pp. 113.

Graidist, P, Plongbunjong, V, Wichienchot, S, Madla, S, Bunyapipat, P & Knudsen, KEB 2019, 'Isomalto-oligosaccharides from rice and their potential use as pharma-nutraceuticals in prevention of colon cancer', Functional Foods in Health and Disease, vol. 9, no. 6, pp. 371-383. https://doi.org/10.31989/ffhd.v9i6.598

Leach, S, Jones, NC , Hoffmann, SV & Un, S 2019, 'Isoquinoline Gas-Phase Absorption Spectrum in the Vacuum Ultraviolet between 3.7 and 10.7 eV. New Valence and Rydberg Electronic States', RSC Advances, vol. 9, pp. 5121-5141. https://doi.org/10.1039/c8ra09725a

Stefánsson, A, Arnórsson, S, Sveinbjörnsdóttir, ÁE, Heinemeier, J & Kristmannsdóttir, H 2019, 'Isotope (δD, δ¹⁸O, ³H, δ¹³C, ¹⁴C) and chemical (B, Cl) Constrains on water origin, mixing, water-rock interaction and age of low-temperature geothermal water', Applied Geochemistry, vol. 108, no. September, 104380. https://doi.org/10.1016/j.apgeochem.2019.104380

Fischer, KFF , Roth, N & Iversen, BB 2019, 'Isotropic Intra-layer Resistivity in Orthorhombic LaSb2 due to an Imperfect Structure', ECT 2019 - 17th European Conference on Thermoelectrics, Limassol, Cyprus, 23/09/2019 - 25/09/2019.

Jing, S , Kryger, P & Boelt, B 2019, Is red clover seed set potential influenced by pollen quality? in NP Anderson (ed.), Proceedings of the Tenth International Herbage Seed Conference. pp. 51-54, 10th International Herbage Seed Group Conference, Corvallis, Oregon, United States, 12/05/2019. <https://ihsg.org/publications/conference-proceedings/#more-101>

Fibiger, MQ 2019, I Sri Lanka går etniske grupper på tværs af religioner. Religion.dk. <https://www.religion.dk/religionslektor-i-sri-lanka-gaar-etniske-grupper-paa-tvaers-af-religioner>

Borup, J 2019, 'I Sri Lanka kæmper de politiske buddhister med næb og kløer mod muslimers indflydelse', Kristeligt Dagblad, vol. Sektion 1, e70c6c38.

Dongmei, Z & Møller, A (eds) 2019, ISSTA 2019: Proceedings of the 28th ACM SIGSOFT International Symposium on Software Testing and Analysis. Association for Computing Machinery, New York. <https://dl.acm.org/citation.cfm?id=3293882&picked=prox>

Green-Pedersen, C 2019, Issue attention in West European Party Politics: CAP and CMP coding compared. in F Baumgartner, C Breunig & E Grossman (eds), Comparative Policy Agendas: Theory, Tools, Data. Oxford University Press, Oxford, pp. 373-390. https://doi.org/10.1093/oso/9780198835332.003.0034

Tromborg, MW 2019, 'Issue Salience and Candidate Position Taking in Parliamentary Parties', Political Studies, vol. 67, no. 2, pp. 307-325. https://doi.org/10.1177/0032321718765520

Andersen, KL 2019, ' Stemmer fra Siden: "For mig har det altid været alt eller intet"', STOF - Viden om rusmidler og samfund, no. 33, pp. 55-59.

Andersen, KL 2019, 'Stemmer fra Siden ”Min hjerne og jeg blev forelsket i OxyContin”', STOF - Viden om rusmidler og samfund, no. 34, pp. 36-41.

Bundgaard, K & Christensen, TP 2019, 'Is the concordance feature the new black? A workplace study of translators' interaction with translation resources while post-editing TM and MT matches', Journal of Specialised Translation, vol. 31, no. 31, pp. 14-37. <http://www.jostrans.org/issue31/art_bundgaard.pdf>

Damgaard, C 2019, 'Is the degree of spatial aggregation of Calluna vulgaris regulated by nitrogen deposition or precipitation?', Acta Oecologica, vol. 97, pp. 1–5. https://doi.org/10.1016/j.actao.2019.04.002

Hatherley, JJ 2019, 'Is the exclusion of psychiatric patients from access to physician-assisted suicide discriminatory?', Journal of Medical Ethics, vol. 45, no. 12, pp. 817-820. https://doi.org/10.1136/medethics-2019-105546

De Masi, C, Vincenzo, F, Matteucci, F, Rosani, G, La Barbera, F, Pasquali, A & Spitoni, E 2019, 'Is the IMF in ellipticals bottom-heavy? Clues from their chemical abundances', Monthly Notices of the Royal Astronomical Society, vol. 483, no. 2, pp. 2217-2235. https://doi.org/10.1093/mnras/sty3127

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2004

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen