Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Bertram, HCS & Lindahl, EI, Fast eller flydende - hvad betyder madens struktur for næringsoptaget?, 2019, Interactive production, YouTube. <https://www.youtube.com/watch?v=dFfOV_ByCr4&feature=youtu.be>

Chatterjee, K, Goharshady, AK, Goyal, P, Ibsen-Jensen, R & Pavlogiannis, A 2019, 'Faster algorithms for dynamic algebraicqueries in basic RSMs with constant treewidth', ACM Transactions on Programming Languages and Systems, vol. 41, no. 4, 23. https://doi.org/10.1145/3363525

Gordon, AV , Rohrbeck, R & Schwarz, JO 2019, 'Faster Horses Won’t Work: Bridging Strategic Foresight and Design-Based Innovation', Paper presented at ISPIM Connnects Ottawa, Ottawa, Canada, 08/04/2019 - 10/04/2019.

Thomsen, IK , Boelt, B , Hansen, EM , Olesen, JE , Sørensen, JN & Kristensen, T 2019, 'Fastlæggelse af dyrkningsperiode for landbrugsafgrøder under danske forhold', No. 2019-760-001228, 12 p., Mar 28, 2019..

Mørup, SH 2019, 'Fastsættelse af ekspropriationserstatning - med eller uden moms?', Ugeskrift for Retsvæsen, vol. 2019, U.2019.B.157, pp. 157-167. <https://pro.karnovgroup.dk/document/7000838230/1>

Gislum, R , Thomsen, IK , Hansen, EM & Olesen, JE 2019, 'Fastsættelse af NDVI-krav i pilotprojektet om biomasse', No. 2019-760-001133, 8 p., May 22, 2019..

Pryds, K, Rasmussen, LE & Andersen, NH 2019, 'Fatal right-sided heart failure due to leukostasis in a patient with leukemic transformation of myelodysplastic syndrome', Clinical Case Reports, vol. 7, no. 1, pp. 115-119. https://doi.org/10.1002/ccr3.1929

Lauridsen, C 2019, 'Fat digestibility in pigs', Nutrition in transition 2019, Nijkerk, Netherlands, 26/11/2019 - 27/11/2019.

Xu, J-X, Li, X-M, Sun, G-X, Cui, L, Ding, L-J, He, C, Li, L-G, Shi, Q, Smets, BF & Zhu, Y-G 2019, 'Fate of Labile Organic Carbon in Paddy Soil Is Regulated by Microbial Ferric Iron Reduction', Environmental Science and Technology, vol. 53, no. 15, pp. 8633-8542. https://doi.org/10.1021/acs.est.9b01323

Arnstad, ED, Glerup, M, Rypdal, V, Peltoniemi, S, Ekelund, M, Berntson, L, Fasth, A, Nielsen, S, Zak, M, Aalto, K, Nordal, E, Herlin, T, Romundstad, PR & Rygg, M 2019, 'Fatigue in juvenile idiopatic arthritis after 18 years of follow-up.', Annals of the Rheumatic Diseases, vol. 78, no. suppl 2, pp. 176. https://doi.org/10.1136/annrheumdis-2019-eular.4006

Baumgartner, J, Yıldırım, HC & Barsoum, Z 2019, 'Fatigue strength assessment of TIG-dressed welded steel joints by local approaches', International Journal of Fatigue, vol. 126, pp. 72-78. https://doi.org/10.1016/j.ijfatigue.2019.04.038

Kristensen, AM , Nielsen, OB , Pedersen, TH & Overgaard, K 2019, 'Fatiguing stimulation increases curvature of the force-velocity relationship in isolated fast-twitch and slow-twitch rat muscles', Journal of Experimental Biology, vol. 222, no. 15, jeb204545 . https://doi.org/10.1242/jeb.204545

Thorup, M, Fat penge, 2019, Pictures, Video and sound recordings (digital), Alphafilm & Kommunikation. <http://alphafilm.dk/fat-penge/>

Sousa, AM, Andrade, TA, Errico, M, Coelho, JP, Filipe, RM & Matos, HA 2019, 'Fatty Acid Content in Biomasses: State-of-the-Art and Novel Physical Property Estimation Methods', International Journal of Chemical Engineering, vol. 2019, 2430234. https://doi.org/10.1155/2019/2430234

Jensen, BS 2019, 'Fatty acid-stimulated uncoupling mitochondrial metabolism increases during arousal from hibernation in the 13-lined ground squirrel', International Congress of Comparative Physiology and Biochemistry (ICCPB), Ottawa, Canada, 05/08/2019 - 08/08/2019.

Georgieva, M, Wei, Y, Dumitrascuta, M, Pertwee, R, Finnerup, NB & Huang, W 2019, 'Fatty acid suppression of glial activation prevents central neuropathic pain after spinal cord injury', Pain, vol. 160, no. 12, pp. 2724-2742. https://doi.org/10.1097/j.pain.0000000000001670

Knorr, S, Bytoft, B, Lohse, Z, Boisen, AB, Clausen, TD, Jensen, RB, Damm, P, Beck-Nielsen, H, Holst, JJ, Hartmann, B, Møller, HJ, Højlund, K, Gravholt, CH & Jensen, DM 2019, 'Fatty Liver Among Adolescent Offspring of Women With Type 1 Diabetes (the EPICOM Study)', Diabetes Care, vol. 42, no. 8, pp. 1560-1568. https://doi.org/10.2337/dc19-0571

Dalgaard, T 2019, Favrskov Kommune: natur og landskab : udnyttelsen af landskabet i dag. in NE Koch (ed.), Trap Danmark: Silkeborg, Favrskov, Skanderborg. 6 edn, vol. 11, Gad, København, pp. 146-148.

Noel, L , Zarazua de Rubens, G , Sovacool, B & Kester, J 2019, 'Fear and loathing of electric vehicles: The reactionary rhetoric of range anxiety', Energy Research & Social Science, vol. 48, pp. 96-107. https://doi.org/10.1016/j.erss.2018.10.001

Niepel, K, Kamp, F, Kurz, C, Hansen, D, Rit, S, Neppl, S, Hofmaier, J, Bondesson, D, Thieke, C, Dinkel, J, Belka, C, Parodi, K & Landry, G 2019, 'Feasibility of 4DCBCT-based proton dose calculation: An ex vivo porcine lung phantom study', Zeitschrift fur Medizinische Physik, vol. 29, no. 3, pp. 249-261. https://doi.org/10.1016/j.zemedi.2018.10.005

Gaballa, K, Denewer, A, Khater, A, Gallotta, V, Conte, C, Federico, A, Elfeki, H & Scambia, G 2019, 'Feasibility of early postoperative bladder catheter removal without prior bladder-training exercises after laparoscopic nerve sparing radical hysterectomy', Journal of Obstetrics and Gynaecology, vol. 39, no. 6, pp. 788-792. https://doi.org/10.1080/01443615.2019.1584883

Janssen, NNY, van Seijen, M, Loo, CE, Vrancken Peeters, MTFD, Hankel, T, Sonke, JJ & Nijkamp, J 2019, 'Feasibility of Micro-Computed Tomography Imaging for Direct Assessment of Surgical Resection Margins During Breast-Conserving Surgery', Journal of Surgical Research, vol. 241, pp. 160-169. https://doi.org/10.1016/j.jss.2019.03.029

Bach Jønsson, A , Møller Thygesen, M , Krogh, S & Kasch, H 2019, 'Feasibility of predicting improvements in motor function following SCI using the SCAR outcome measure: a retrospective study', Spinal Cord, vol. 57, no. 11, pp. 966-971. https://doi.org/10.1038/s41393-019-0307-z

Hansen, CP, Moghiseh, M, Oland, C, Poulsen, I & Sabers, A 2019, 'Feasibility of Transcutaneous Vagus Nerve Stimulation in Patients with Severe Traumatic Brain Injury', pp. 150-150.

Bangsgaard, P, Yeomans, L, Darabi, H, Gregersen, KM, Olsen, J, Richter, T & Mortensen, P 2019, 'Feasting on Wild Boar in the Early Neolithic. Evidence from an 11,400-year-old Placed Deposit at Tappeh Asiab, Central Zagros', Cambridge Archaeological Journal, vol. 29, no. 3, pp. 443-463. https://doi.org/10.1017/S095977431900009X

Ntakaris, A, Mirone, G, Kanniainen, J, Gabbouj, M & Iosifidis, A 2019, 'Feature Engineering for Mid-Price Prediction with Deep Learning', IEEE Access, vol. 7, 8743410, pp. 82390 - 82412. https://doi.org/10.1109/ACCESS.2019.2924353

Rørstad, JJ 2019, 'Features of attosecond transient-absorption spectra'.

Curtasu, MV , Knudsen, KEB , Hedemann, MS , Lærke, HN , Xu, Y, Tafintseva, V, Kohler, A, Marco, M & Bendiks, Z 2019, 'Fecal metabolome and microbiota composition in obese Gottingen Minipigs as influecned by source of carbohydrates', Metabolomics 2019: 15th Anual Conference of the Metabolomics Society, The Hague, Netherlands, 23/06/2019 - 27/06/2019.

Curtasu, MV, Tafintseva, V, Bendiks, Z, Marco, ML, Kohler, A, Xu, Y, Lærke, HN , Knudsen, KEB & Hedemann, MS 2019, Fecal metabolome and microbiota composition in obese Göttingen minipigs as influenced by source of carbohydrates. in Proceedings of the 15th Annual Conference of the Metabolomics Society. pp. 135-135, Metabolomics 2019: 15th Anual Conference of the Metabolomics Society, The Hague, Netherlands, 23/06/2019.

Hvas, CL , Jørgensen, SMD , Jørgensen, SP , Storgaard, M , Lemming, L , Hansen, MM , Erikstrup, C & Dahlerup, JF 2019, 'Fecal Microbiota Transplantation is Superior to Fidaxomicin for Treatment of Recurrent Clostridium difficile Infection', Gastroenterology, vol. 156, no. 5, pp. 1324-1332.e3. https://doi.org/10.1053/j.gastro.2018.12.019

Sun, D, Huang, Z, Zhuang, Z, Ma, Z, Man, LK, Liao, D & Gregersen, H 2019, 'Fecobionics: A Novel Bionics Device for Studying Defecation', Annals of Biomedical Engineering, vol. 47, no. 2, pp. 576-589. https://doi.org/10.1007/s10439-018-02149-1

Wildenschild, C , Riis, AH , Ehrenstein, V, Hatch, EE, Wise, LA, Rothman, KJ, Sørensen, HT & Mikkelsen, EM 2019, 'Fecundability among Danish women with a history of miscarriage: a prospective cohort study', BMJ Open, vol. 9, no. 1, e023996. https://doi.org/10.1136/bmjopen-2018-023996

Brøgger, K & Karseth, B 2019, 'Federal, state or market governance – education quality at risk? The emergence of an accreditation market in European higher education.', ECER 2019, Hamburg, Hamburg, Germany, 03/09/2019 - 06/09/2019.

Lauridsen, C 2019, 'Feed additives as alternatives to zinc oxide to reduce post-weaning diarrhoea', Zero Zinc Summit, København, Denmark, 17/06/2019 - 18/06/2019.

Sortkær, B 2019, 'Feedback, elevperspektiver og ulighed i skolen', Aarhus.

Sortkær, B 2019, 'Feedback for Everybody? Exploring the relationship between students’ perceptions of feedback and students’ socioeconomic status', British Educational Research Journal, vol. 45, no. 4, pp. 717-735. https://doi.org/10.1002/berj.3522

Østergård, ORK 2019, 'Feedback informed treatment: effectiveness and moderators', Aarhus. <https://soeg.kb.dk/permalink/45KBDK_KGL/1pioq0f/alma99123484743005763>

Børsting, CF , Hellwing, ALF , Weisbjerg, MR , Østergaard, S, Raun, BL, Røjen, BA & Kristensen, NB 2019, Feeding dairy cows with no or reduced amounts of forage. in P Udén, T Eriksson, R Spörndly, B-O Rustas & J Karlsson (eds), Proceedings of the 10th Nordic Feed Science Conference. Swedish University of Agricultural Sciences, Department of Animal Nutrition and Management, Uppsala, Sveriges Lantbruksuniversitet. Institutionen foer Husdjurens Utfordring och Vaard. Rapport, vol. 302, pp. 87-91, Nordic Feed Science Conference, Uppsala, Sweden, 11/06/2019. <https://www.slu.se/globalassets/ew/org/inst/huv/nfsc/nfsc2019/nfsc-proceedings-2019-06-05-torsten-eriksson.pdf>

Grønkjær, P , Nielsen, KV , Zoccarato, G , Meire, L , Rysgaard, S & Hedeholm, RB 2019, 'Feeding ecology of capelin (Mallotus villosus) in a fjord impacted by glacial meltwater (Godthåbsfjord, Greenland)', Polar Biology, vol. 42, no. 1, pp. 81-98. https://doi.org/10.1007/s00300-018-2400-8

Xydis, G & Vlachakis, N 2019, 'Feed-in-premium renewable energy support scheme: A scenario approach', Resources, vol. 8, no. 2, 106. https://doi.org/10.3390/resources8020106

Olijhoek, D , Løvendahl, P , Hellwing, ALF , Weisbjerg, MR & Lund, P 2019, 'Feed sorting of low and high concentrate diets by Holstein and Jersey dairy cows', British Society of Animal Science conference 2019, Edinburgh, United Kingdom, 09/04/2019 - 11/04/2019 pp. 199.

Sidenius, A, Mogensen, O, Rudnicki, M, Møller, LMA & Hansen, HP 2019, 'Feeling lucky: hierarchies of suffering and stories of endometrial cancer in a Danish context', Sociology of Health and Illness, vol. 41, no. 5, pp. 950-964. https://doi.org/10.1111/1467-9566.12875

Holm, C 2019, 'Fejl bedre!', Ledelse i Morgen - Tidsskrift for pædagogisk ledelse, vol. 22, pp. 5-7.

Knoop, HH 2019, Fejl og fejl: og når den største fejl er ikke at turde begå fejl. in R Bøgelund (ed.), Fejl: et opgør med nulfejlstyranniet og den usunde præstationskultur. Muusmann forlag, København, pp. 5-8.

Greve, MH , Pedersen, BF , Greve, MB & Gyldenkærne, S 2019, 'Fejl om udbredelse af organiske jorde – Del 2', No. 2019-760-001321, 5 p., Nov 21, 2019..

Munkholm, NV 2019, 'Fel beräkna förmåner vid uppsägning på föräldraledigs deltidslön', EU & Arbetsrätt : nordiskt nyhetsbrev, no. 2, pp. 7. <http://arbetsratt.juridicum.su.se/euarb/19-02/NR%20219%20slutlig.pdf>

Tjørnhøj-Thomsen, T, Whyte, SR & Høybye, MT 2019, Feltarbejde og deltagerobservation. in AMB Jensen & S Vallgårda (eds), Forskningsmetoder i folkesundhedsvidenskab. 5 edn, Munksgaard , København, pp. 95-122.

Esteves, SC , Humaidan, P, Roque, M & Agarwal, A 2019, 'Female infertility and assisted reproductive technology', Panminerva medica, vol. 61, no. 1, pp. 1-2. https://doi.org/10.23736/S0031-0808.18.03553-X

Thyø, A , Elfeki, H , Laurberg, S & Emmertsen, KJ 2019, 'Female sexual problems after treatment for colorectal cancer - a population-based study', Colorectal Disease, vol. 21, no. 10, pp. 1130-1139. https://doi.org/10.1111/codi.14710

Willis, SK, Hatch, EE, Wesselink, AK, Rothman, KJ, Mikkelsen, EM & Wise, LA 2019, 'Female sleep patterns, shift work, and fecundability in a North American preconception cohort study', Fertility and Sterility, vol. 111, no. 6, pp. 1201-1210.e1. https://doi.org/10.1016/j.fertnstert.2019.01.037

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