Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Kiilerich, AH & Molmer, K 2019, 'Input-Output Theory with Quantum Pulses', Physical Review Letters, vol. 123, no. 12, 123604. https://doi.org/10.1103/PhysRevLett.123.123604

Hejl, JL, Grand, MK, Siersma, V, Goetze, JP, de Fine Olivarius, N, Andersen, CL & Lind, B 2019, 'In Reply', Clinical Chemistry, vol. 65, no. 6, pp. 812-813. https://doi.org/10.1373/clinchem.2019.302034

Keall, PJ, Nguyen, DT, O'Brien, R, Zhang, P, Bertholet, J & Poulsen, PR 2019, 'In Reply to Dahele and Verbakel', International Journal of Radiation Oncology, Biology, Physics, vol. 103, no. 1, pp. 283-284. https://doi.org/10.1016/j.ijrobp.2018.08.063

Jeppesen, J , Johansen, P & Beniczky, S 2019, 'In response: Heart rate differential method simple but inefficient method for seizure detection', Epilepsia, vol. 60, no. 12, pp. 2532. https://doi.org/10.1111/epi.16391

Mehlum, CS, Groentved, AM, Godballe, C, Larsen, SR, Kiss, K, Kjaergaard, T & Møller, S 2019, 'In response to Laryngeal precursor lesions: Interrater and intrarater reliability of histopathological assessment', The Laryngoscope, vol. 129, no. 3, pp. E93. https://doi.org/10.1002/lary.27809

Borup, D & Montes Schütte, EC 2019 'In search of a job: Forecasting employment growth using Google Trends' CREATES Research Paper, no. 2019-13, Aarhus, pp. 1.

Mathisen, A, Horak, T, Klokmose, CN , Grønbæk, K & Elmqvist, N 2019, 'InsideInsights: Integrating Data-Driven Reporting in Collaborative Visual Analytics', Computer Graphics Forum, vol. 38, no. 3, pp. 649-661. https://doi.org/10.1111/cgf.13717

Simovska, V , Lagermann, LC , Abduljalil, HSY , Mørck, LL & Kousholt, D 2019, 'Inside out: what we (don’t) talk about when we talk about research', Qualitative Research, vol. 19, no. 2, pp. 113-130. https://doi.org/10.1177/1468794117749165

Firth, A 2019, 'Inside the intelligent multilingual environment: Semiotic assemblages in a Bangkok bazaar', Paper presented at Lunchtime lecture series, Dept. of English, Aarhus University, Aarhus, Denmark, 06/03/2019 - 06/03/2019 pp. 1-30.

Kallestrup-Lamb, M, Kjærgaard, S & Rosenskjold, CPT 2019 'Insight into Stagnating Life Expectancy: Analysing Cause of Death Patterns across Socio-economic Groups' CREATES Research Paper, no. 2019-20, Institut for Økonomi, Aarhus Universitet, Aarhus.

Zhou, Y , Jones, NC , Pedersen, JN, Perez, B, Hoffmann, SV , Petersen, SV , Pedersen, JS, Perriman, A, Kristensen, P, Gao, R & Guo, Z 2019, 'Insight into the structure and activity of surface-engineered lipase biofluids', ChemBioChem, vol. 20, no. 10, pp. 1266-1272. https://doi.org/10.1002/cbic.201800819

Bhattacharyya, S, Mungi, NA, Kawamichi, T, Rawat, GS, Adhikari, BS & Wilkening, JL 2019, 'Insights from present distribution of an alpine mammal Royle’s pika (Ochotona roylei) to predict future climate change impacts in the Himalaya', Regional Environmental Change, vol. 19, no. 8, pp. 2423-2435. https://doi.org/10.1007/s10113-019-01556-x

Elsworth, Y , Hekker, S, Johnson, JA, Kallinger, T, Mosser, B, Pinsonneault, M, Hon, M, Kuszlewicz, J , Miglio, A, Serenelli, A, Stello, D, Tayar, J & Vrard, M 2019, 'Insights from the APOKASC determination of the evolutionary state of red-giant stars by consolidation of different methods', Monthly Notices of the Royal Astronomical Society, vol. 489, no. 4, pp. 4641-4657. https://doi.org/10.1093/mnras/stz2356

Portillo Perez, G, Mukherjee, A & Dumont, MJ 2019, 'Insights into HMF catalysis', Journal of Industrial and Engineering Chemistry, vol. 70, pp. 1-34. https://doi.org/10.1016/j.jiec.2018.10.002

Thomsen, MK , Nyvang, A, Walsh, JPS, Bunting, PC, Long, JR, Neese, F, Atanasov, M, Genoni, A & Overgaard, J 2019, 'Insights into Single-Molecule-Magnet Behavior from the Experimental Electron Density of Linear Two-Coordinate Iron Complexes', Inorganic Chemistry, vol. 58, no. 5, pp. 3211-3218. https://doi.org/10.1021/acs.inorgchem.8b03301

Zhang, J , Song, L & Iversen, BB 2019, 'Insights into the design of thermoelectric Mg3Sb2 and its analogs by combining theory and experiment', npj Computational Materials, vol. 5, no. 1, 76. https://doi.org/10.1038/s41524-019-0215-y

Cavallero, S, Nejsum, P, Cutillas, C, Callejón, R, Doležalová, J, Modrý, D & D'Amelio, S 2019, 'Insights into the molecular systematics of Trichuris infecting captive primates based on mitochondrial DNA analysis', Veterinary Parasitology, vol. 272, pp. 23-30. https://doi.org/10.1016/j.vetpar.2019.06.019

Thomsen, MK , Nyvang, A , Overgaard, J, Walsh, JPS, Bunting, PC, Long, JR, Neese, F, Atanasov, M & Genoni, A 2019, 'Insights into the Origin of Magnetic Anisotropy in Linear Iron Complexes from the Experimental Electron Density', ECM 32 - 32nd European Crystallographic Meeting, Vienna, Austria, 18/08/2019 - 23/08/2019.

Wolf Pérez, A-M 2019, 'In silico and in vitro biophysical screening methods for proteins'.

Khawaja, I , Kousholt, D & Mathiassen, C 2019, 'Insisterende nytænkning af inklusion, fællesskab og følelser', Nordiske Udkast, vol. 46, no. 1.

Vergeynst, L, Christensen, JH, Kjeldsen, KU , Meire, L, Boone, W, Malmquist, LMV & Rysgaard, S 2019, 'In situ biodegradation, photooxidation and dissolution of petroleum compounds in Arctic seawater and sea ice', Water Research, vol. 148, pp. 459-468. https://doi.org/10.1016/j.watres.2018.10.066

Lagarteira, T, Pacheco, P, Almeida, C, Bentien, A, Monteiro, R & Mendes, A 2019, 'In-Situ Measurement of Vanadium Crossover for the Vanadium Redox Flow Battery', Journal of The Electrochemical Society, vol. 166, no. 16, pp. A4067-A4072. https://doi.org/10.1149/2.0331916jes

Kofoed, MVW 2019, 'In situ Methnation: Effect of Hydrogen on Acetate Production and Consumption Rates', 16th IWA World Conference on Anaerobic Digestion, Delft, Netherlands, 23/06/2019 - 26/06/2019.

Thevenon, A, Rosas-Hernández, A, Peters, JC & Agapie, T 2019, 'In-Situ Nanostructuring and Stabilization of Polycrystalline Copper by an Organic Salt Additive Promotes Electrocatalytic CO₂ Reduction to Ethylene', Angewandte Chemie - International Edition, vol. 58, no. 47, pp. 16952-16958. https://doi.org/10.1002/anie.201907935

Nosrati, H, Mamoory, R, Dabir, F, Perez, MC, Rodriguez, MA, Le, DQS & Bünger, C 2019, 'In situ synthesis of three dimensional graphene-hydroxyapatite nano powders via hydrothermal process', Materials Chemistry and Physics, vol. 222, pp. 251-255. https://doi.org/10.1016/j.matchemphys.2018.10.023

Roelsgaard, M, Dippel, A-C, Song, L , Blichfeld, AB & Iversen, BB 2019, 'In situ total scattering studies during growth of thin films by magnetron sputtering', Conference on Analysis of Diffraction Data in Real Space, Grenoble, France, 20/03/2019 - 22/03/2019.

Søndergaard-Pedersen, FM , Broge, NLN , Broge, NLN , Yu, J , Mamakhel, MAH , Beyer, J & Iversen, BB 2019, 'In situ X-ray scattering study of hydrothermal synthesis of anatase TiO2 nanoparticles from commercial precursor TiOSO4', ECM 32 - 32nd European Crystallographic Meeting, Vienna, Austria, 18/08/2019 - 23/08/2019.

Niemistö, J & Lund-Hansen, LC 2019, 'Instantaneous effects of sediment resuspension on inorganic and organic benthic nutrient fluxes at a shallow water coastal site in the Gulf of Finland, Baltic Sea', Estuaries and Coasts, vol. 42, no. 8, pp. 2054–2071. https://doi.org/10.1007/s12237-019-00648-5

Finke, D 2019, 'Institutional conditions for effective parliamentary oversight of European Union politics', West European Politics, vol. 42, no. 1, pp. 1-24. https://doi.org/10.1080/01402382.2018.1474418

Jørgensen, PEF 2019, 'Institutional Identity as Discourse: Explaining What We Are Made of', I-LanD International Journal, vol. 2019, no. 1, pp. 8-24.

Demangeot, C, Kipnis, E, Pullig, C, Cross, SNN, Larsen, LV, Cui, C, Williams, JD, Galalae, C, Licsandru, T, Kearney, S, Mari, C, Martin Ruiz, V & Swanepoel, S 2019, 'Institutionalising Intercultural Engagement in Multicultural Marketplaces: Developing TCR- led Interventions with the New Generation', 2019 Transformative Consumer Research Conference, Tallahssee, United States, 19/05/2019 - 21/05/2019 pp. 49-50. <https://www.mytcr.org/_files/ugd/b4cd18_f2d165df904941f08b43bd2e59ef3c1a.pdf>

Stounbjerg, P 2019, Institutional settings: introduction to section 4. in B Hjartarson, A Kollnitz, P Stounbjerg & T Ørum (eds), A cultural history of the avant-garde in the Nordic Countries 1925-1950. Brill, Leiden, Avant Garde Critical Studies, no. 36, pp. 477-486. https://doi.org/10.1163/9789004388291_027

Holm, C 2019, 'Institutleder: Man bliver ikke fuld af faglig trivsel af at drikke øl – man bliver bare fuld', Politiken. <https://politiken.dk/debat/debatindlaeg/art7355022/Man-bliver-ikke-fuld-af-faglig-trivsel-af-at-drikke-%C3%B8l-%E2%80%93-man-bliver-bare-fuld>

Uusimäki, E 2019, Instruction (4QInstruction). in LT Stuckenbruck & DM Gurtner (eds), T&T Clark Encyclopaedia of Second Temple Judaism. vol. 1, Bloomsbury T&T Clark, London, pp. 1:247-249.

Andersen, SC , Guul, TS & Humlum, MK 2019 'Instruction Time, Information, and Student Achievement: Evidence from a Field Experiment'.

Vila, G, Jorgensen, JOL, Luger, A & Stalla, GK 2019, 'Insulin Resistance in Patients With Acromegaly', Frontiers in Endocrinology, vol. 10, 509. https://doi.org/10.3389/fendo.2019.00509

Wiggers, H 2019, 'Insulin treatment in heart failure patients: the good, the bad or the ugly?', European Journal of Heart Failure, vol. 21, no. 8, pp. 985-987. https://doi.org/10.1002/ejhf.1556

Bork, CS, Lasota, AN, Lundbye-Christensen, S, Jakobsen, MU, Tjønneland, A, Calder, PC, Schmidt, EB & Overvad, K 2019, 'Intake of α-linolenic acid is not consistently associated with a lower risk of peripheral artery disease: results from a Danish cohort study', The British Journal of Nutrition, vol. 122, no. 1, pp. 86-92. https://doi.org/10.1017/S0007114519000874

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Czamara, D, Eraslan, G, Page, CM, Lahti, J, Lahti-Pulkkinen, M, Hämäläinen, E, Kajantie, E, Laivuori, H, Villa, PM, Reynolds, RM, Nystad, W, Håberg, SE, London, SJ, O'Donnell, KJ, Garg, E, Meaney, MJ, Entringer, S, Wadhwa, PD, Buss, C, Jones, MJ, Lin, DTS, MacIsaac, JL, Kobor, MS, Koen, N, Zar, HJ, Koenen, KC, Dalvie, S, Stein, DJ, Kondofersky, I, Müller, NS, Theis, FJ, Major Depressive Disorder Working Group of the Psychiatric Genomics Consortium, Räikkönen, K & Binder, EB 2019, 'Integrated analysis of environmental and genetic influences on cord blood DNA methylation in new-borns', Nature Communications, vol. 10, 2548. https://doi.org/10.1038/s41467-019-10461-0

Kronvang, B , Zak, DH , Carstensen, MV, Jensen, HS & van't Veen, SGW 2019, 'Integrated buffer zones as a new mitigation measure for enhancing nutrient retention', EGU General Assembly 2019, Vienna, Austria, 07/04/2019 - 12/04/2019.

Boateng, MA , Angel, S & Enemark, U 2019, 'Integrated Community Case Management of Malaria-Stakeholders' Assessment From a Health Literacy Perspective', 24th Annual Qualitative Health Research Conference, Halifax, Novo Scottia, CA, 22/10/2018.

Khataee, A, Azevedo, J, Dias, P, Ivanou, D, Dražević, E , Bentien, A & Mendes, A 2019, 'Integrated design of hematite and dye-sensitized solar cell for unbiased solar charging of an organic-inorganic redox flow battery', Nano Energy, vol. 62, pp. 832-843. https://doi.org/10.1016/j.nanoen.2019.06.001

K. Zadora, P, Chumduri, C, Imami, K, Berger, H, Mi, Y, Selbach, M, Meyer, TF & Kumar Gurumurthy, R 2019, 'Integrated Phosphoproteome and Transcriptome Analysis Reveals Chlamydia-Induced Epithelial-to-Mesenchymal Transition in Host Cells', Cell Reports, vol. 26, no. 5, pp. 1286-1302. https://doi.org/10.1016/j.celrep.2019.01.006

Heine, C 2019, 'Integrated Problem Decision Reports in Schreibdidaktik und Beratung', JoSch - Journal der Schreibberatung, vol. 17, no. 01, pp. 48-55. https://doi.org/10.3278/JOS1901W048

Heine, C 2019, 'Integrated Problem Decision Reports (IPDR) - Ein Unterrichtswerkzeug aus der Übersetzungswissenschaft', 7. Bremer Symposion: Rethinking the Language Learner. Paradigmen - Methoden - Disziplinen, Bremen, Germany, 28/02/2019 - 02/03/2019. <https://www.fremdsprachenzentrum-bremen.de/fileadmin/autor/bilder/Aktuelles/Symposion_2019/Folien_4/4_Heine.pdf>

Raja, AS, Liu, J, Volet, N, Wang, RN, He, J, Lucas, E, Bouchand, R, Morton, P, Bowers, J & Kippenberg, TJ 2019, 'Integrated Si3N4 Soliton Microcomb Driven by a Compact Ultra-low-noise Laser', Conference on Lasers and Electro-Optics.

Garg, V, Khan, AW, Kudapa, H, Kale, SM, Chitikineni, A, Qiwei, S, Sharma, M, Li, C, Zhang, B, Xin, L, Kishor, PBK & Varshney, RK 2019, 'Integrated transcriptome, small RNA and degradome sequencing approaches provide insights into Ascochyta blight resistance in chickpea', Plant Biotechnology Journal, vol. 17, no. 5, pp. 914-931. https://doi.org/10.1111/pbi.13026

Tao, S, Xu, Q, Feijóo, A, Kuenzel, S & Bokde, N 2019, 'Integrated wind farm power curve and power curve distribution function considering the wake effect and terrain gradient', Energies, vol. 12, no. 13, 2482. https://doi.org/10.3390/en12132482

Benjamin, J, McCarthy, J, Wiseman, C, Bevin, S, Kowlessar, J, Astrup, PM, Naumann, J & Hacker, J 2019, Integrating Aerial and Underwater Data for Archaeology: Digital Maritime Landscapes in 3D. in Coastal Research Library. Springer, Coastal Research Library, vol. 31, pp. 211-231. https://doi.org/10.1007/978-3-030-03635-5_14

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