Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Rasmussen, BS 2003, 'Choosing between different fixed exchange rate regimes', Journal of Financial Transformation, vol. 7, pp. 31-38.

Hansen, PR, Lunde, A & Nason, JM 2003, 'Choosing the Best Volatility Models: The Model Confidence Set Approach', Oxford Bulletin of Economics and Statistics, vol. 65, pp. 839-861.

Nissen, UB 2003, 'Christ and the world', Journal of Lutheran Ethics , vol. 3, no. 8.

Mührmann-Lund, J 2003, 'Christian Güldencrones rejsebog: En undersøgelse af synet på forholdet mellem forfatter, stof og læsere belyst ved en rejseberetning fra 1700-tallet', Rotunden, no. 18, pp. 4-25.

Bolwig, NG & Yndgaard, E 2003, Christian III's rentemesterregnskaber, bind V: Ordforklaringer, sagregister og stikordsregister. Kildeskriftselskabet.

Sørensen, JS 2003, Christian Theology and Intellectuals in China. Center for Multireligiøse Studier, Aarhus Universitet, Århus.

Kerndrup, GB & Kjeldsen, E 2003, 'Chromosome analysis/cytogenetic analysis in neoplasms and hereditary diseases', Ugeskrift for Læger, vol. 9, no. 165, pp. 892-897.

Madsen, LB , Petersen, AH , Nielsen, VH , Nissen, PH , Dunø, M , Krejci, L , Bendixen, C & Thomsen, B 2003, 'Chromosome location, genomic organization of the porcine COL10A1 gene and model structure of the NC1 domain', Cytogenetic and Genome Research, vol. 102, no. 1-4, pp. 173-178. <http://content.karger.com/ProdukteDB/produkte.asp?Aktion=ShowAbstract&ArtikelNr=75744&Ausgabe=229862&ProduktNr=224037>

Pitt, AD, Fernandes, C, Bewick, NL, Hemsworth, PD, Buhagiar, KA, Hansen, PS, Rasmussen, HH, Delbridge, L & Whalley, DW 2003, 'Chronic amiodarone-induced inhibition of the Na+-K+pump in rabbit cardiac myocytes in thyroid-dependent: comparison with dronedarone', Cardiovascular Research, vol. 57, no. 1, pp. 101-108.

Licht, RW, Larsen, JO, Smith, D & Brændgaard, H 2003, 'Chronic lithium treatment with or without haloperidol fails to affect the morphology of the rat cerebellum', European Neuropsychopharmacology, vol. 13, no. 3, pp. 173-176. https://doi.org/10.1016/S0924-977X(03)00003-8

Graebe, M, Brond, L, Christensen, S, Nielsen, S, Olsen, NV & Jonassen, TE 2003, 'Chronic nitric oxide synthase inhibition exacerbates renal dysfunction in cirrhotic rats', American Journal of Physiology: Renal Physiology, vol. 0, pp. 0-0.

Bro, S, Bentzon, JF , Falk, E, Andersen, CB, Ølgaard, K & Nielsen, LB 2003, 'Chronic renal failure accelerates atherogenesis in apolipoprotein e-deficient mice', Journal of the American Society of Nephrology, vol. 14, no. 10, pp. 2466-2474.

Siedersleben, W & Dahl, B 2003, Chronology of education and training policy within the European Union. in D Phillips & H Ertl (eds), Implementing European Union Education and Training Policy - A Comparative Study of Issues in Four Member States. Kluwer Academic Publishers, Dorcrecht, pp. 319-329.

Aubry, M-P, Berggren, WA, Van Couvering, JA, Ali, J, Brinkhuis, H, Cramer, B, Kent, DV, Swisher, CCIII, Dupuis, C, Gingerich, PD, Heilmann-Clausen, C, King, C, Ward.D.J., Knox, RWOB, Ouda, K, Stott, LD & Thiry, M 2003, 'Chronostratigraphic terminology at the Paleocene/Eocene Boundary: In: Wing, S.L., Gingerich, P., Schmitz, B. & Thomas, E. (eds.), Causes and Consequences of Globally Warm Climates in the Early Paleogene', Geological Society of America Special Paper, no. 369, pp. 551-566.

Jakobsen, L, Aaslyng, JM & Rosenqvist, ESK 2003, Chrysanthemum og dynamisk klimastyring: En IntelliGrow rapport.

Finsen, HC 2003, 'Cicero fra Sorø', RetorikMagasinet, vol. 13, no. 49, pp. 18-20.

Nielsen, KH 2003, 'Ciceros udfordring: Et sprogteoretisk essay', Rhetorica Skandinavica, no. 25, pp. 29-44.

Plant, P 2003, Cinq cygnes a 3 dimension: l'orientation scolaire et professionelle dans les pays nordiques.

Lomholt, JA & Kilian, M 2003, 'Ciprofloxacin susceptibility of Pseudomonas aeruginosa isolates from keratitis', British Journal of Ophthalmology, vol. 87, pp. 1238-1240.

Lomholt, JA & Kilian, M 2003, 'Ciprofloxacin susceptibility of Pseudomonas aeruginosa isolates from keratitis', British Journal of Ophthalmology, vol. 87, pp. 1238-40.

Hansen, KA , Miltersen, PB & Vinay, V 2003, Circuits on Cylinders. in Fundamentals of Computation Theory. Springer, Lecture Notes in Computer Science, vol. 2751, pp. 171-182, Symposium on Fundamentalsof Computation Theory. FCT '03, Malmø, Sweden, 13/08/2003. https://doi.org/10.1007/978-3-540-45077-1_17

Østergard, T, Hansen, TK, Nyholm, B, Gravholt, CH, Djurhuus, CB, Hosoda, H, Kangawa, K & Schmitz, O 2003, 'Circulating ghrelin concentrations are reduced in healthy offspring of Type 2 diabetic subjects, and are increased in women independent of a family history of Type 2 diabetes', Diabetologia, vol. 46, no. 1, pp. 134-136.

Østergaard, T, Hansen, TK, Nyholm, B, Gravholt, CH, Djurhuus, CB, Hosoda, H, Kangawa, K & Schmitz, O 2003, 'Circulating ghrelin concentrations are reduced in healthy offspring of Type 2 diabetic subjects, and are increased in women independent of a family history of Type 2 diabetes', Diabetologia, vol. 46, pp. 134-136.

Henschel, RF 2003, CISG art. 35 - mangelsbedømmelsen og kontraktrettens internationalisering. in Kontraktrettens internationalisering. pp. 26 s., Den Nordiske Handelsretslærerkonference i Bergen, Norge, 21/05/2003.

Henschel, RF, Lookofsky, J, Møgelvang-Hansen, P & Kristensen, LH (eds) 2003, CISG Denmark (www.cisg.dk). Handelshøjskolen i Århus, Juridisk Institut, Århus. <http://www.cisg.dk/>

Anderson, S 2003, Civilizing Children: Children's sport and civil sociality in Copenhagen, Denmark. Institut for Antropologi, Københavns Universitet, Kbh.

Nielsen, HK 2003 'Civil Society and Social Solidarity' ACTS nr. 17 edn, Æstetik og Kultur, Aarhus Universitet, Århus.

Amsinck, SL , Jeppesen, E & Ryves, D 2003, 'Cladoceran stratigraphy in two shallow brackish lakes with special reference to changes in salinity, macrophyte abundance and fish predation', Journal of Paleolimnology, vol. 29, pp. 495-507.

Andersen, HR, Sejrsen, K, Kristensen, T, Sørensen, MT, Straarup, EM & Kristensen, T 2003, 'CLA-indhold i komælk', Nyhedsbrev fra Forskningscenter for Økologisk Jordbrug., pp. 4-4.

Fynbo, HOU, Prezado, Y, Bergmann, UC, Borge, MJG, Dendooven, P, Huang, WX, Huikari, J, Jeppesen, H, Jones, P, Jonson, B, Meister, M, Nyman, G, Riisager, K, Tengblad, O, Vogelius, IS , Wang, Y, Weissman, L, Rolander, KW & Äystö, J 2003, 'Clarification of the Three-Body Decay of [Formula presented] (12.71 MeV)', Physical Review Letters, vol. 91, no. 8. https://doi.org/10.1103/PhysRevLett.91.082502

Fynbo, HOU, Prezado, Y, Bergmann, UC, Borge, MJG, Dendooven, P, Huang, WX, Huikari, J, Jeppesen, H, Jones, P, Jonson, B, Meister, M, Nyman, G, Riisager, K, Tengblad, O, Vogelius, IS, Wang, Y, Weissman, L, Wilhelmsen Rolander, K & Äystö, J 2003, 'Clarification of the Three-Body Decay of ¹²C (12.71 MeV)', Physical Review Letters, vol. 91, no. 8, pp. 021905-1.

Fynbo, HOU, Prezado, Y, Bergmann, UC, Borge, MJG, Dendooven, P, Huang, WX, Huikari, J, Jeppesen, H, Jones, P, Jonson, B, Meister, M, Nyman, G, Riisager, K, Tengblad, O, Vogelius, IS , Wang, Y, Weissman, L, Rolander, KW & Äystö, J 2003, 'Clarification of the Three-Body Decay of ¹²C (12.71 MeV)', Physical Review Letters, vol. 91, no. 8, pp. 825021-825024.

Roepstorff, A 2003, Clashing Cosmologies. Contrasting Knowledges in the Greenlandic Fishery. in A Roepstorff, N Bubandt & K Kull (eds), Imagining Nature. Practices of Cosmology and Identity. vol. 68, Aarhus Universitetsforlag, London, pp. 117-42.

Thomsen, O 2003, Classica et Mediaevalia: Revue danoise de philologie et d'histoire. 54 edn.

Andersen, LD 2003, 'Classification of bladder cancer by microarray expression profiling: towards a general clinical use of microarrays in cancer diagnostics', Expert Review of Molecular Diagnostics, vol. 5, no. 3, pp. 635-647.

Andersen, LD 2003, 'Classification of bladder cancer by microarray expression profiling: towards a general clinical use of microarrays in cancer diagnostics'.

Frederiksen, CM, Knudsen, S, Laurberg, S & Ørntoft, TF 2003, 'Classification of Dukes B and C colorectal cancers using expression arrays', J. Cancer Res. Clin. Oncol., pp. 728-734.

Jensen, AS , Riisager, K , Fedorov, DV & Garrido, E 2003, 'Classification of three-body quantum halos', Europhysics Letters, vol. 61, pp. 320-326.

Lopez, R & Baelum, V 2003, 'Classifying periodontitis among adolescents: implications for epidemiological research', Community Dentistry and Oral Epidemiology, vol. 31, no. 2, pp. 136-43.

Marschner, B 2003, 'Claus Røllum-Larsen: Impulser i Københavns koncertrepertoire 1900-1935: Studier i præsentationen af ny, især udenlandsk instrumentalmusik, I-II', Denmark. Kongelige Bibliotek. Fund og Forskning, pp. 441-441.

Rey, J-F & Kruse, A 2003, 'Cleaning and disinfection in Europe accordin to the Endoscopic Societies` Guideline', Endoskopie, vol. 35, pp. 878-881.

Petersen, JK , Sejr, MK & Larsen, J 2003, 'Clearance rates in the Arctic bivalves Hiatella arctica and Mya sp.', Polar Biology, vol. 26, pp. 334-341.

Petersen, JK , Sejr, MK & Larsen, JEN 2003, 'Clearance rates in the Arctic bivalves Hiatella arctica and Mya sp.', Polar Biology, vol. 26, no. 5, pp. 334-341.

Olsen, RK, Andresen, BS, Christensen, E, Bross, P, Skovby, F & Gregersen, N 2003, 'Clear relationship between ETF/ETFDH genotype and phenotype in patients with multiple acyl-CoA dehydrogenation deficiency', Human Mutation, no. 22, pp. 12-23.

Olsen, RKJ , Andresen, BS, Christensen, E, Bross, P, Skovby, F & Gregersen, N 2003, 'Clear relationship between ETF/ETFDH genotype and phenotype in patients with multiple acyl-CoA dehydrogenation deficiency.', Human Mutation, vol. 22, no. 1, pp. 12-23. https://doi.org/10.1002/humu.10226

Stubager, R 2003, Cleavages Reformulated: The Development of a Sociostructurally Anchored Definition. in Ikke angivet. Department of Political Science, University of Aarhus, ECPR Joint Sessions of Workshops, Edinburgh, March 28-April 2, 2003, 17/12/2010.

Kaplan, JO, Bigelow, NH, Prentice, IC, Harrison, SP, Bartlein, PJ, Christensen, TR, Cramer, W, Matveyeva, NV, McGuire, AD, Murray, DF, Razzhivin, VY, Smith, B, Walker, DA, Anderson, PM, Andreev, AA, Brubaker, LB, Edwards, ME & Lozhkin, AV 2003, 'Climate change and Arctic ecosystems: 2. Modeling, paleodata-model comparisons, and future projections', Journal of Geophysical Research: Atmospheres, vol. 108, no. 19. https://doi.org/10.1029/2002JD002559

Bigelow, NH, Brubaker, LB, Edwards, ME, Harrison, SP, Prentice, IC, Anderson, PM, Andreev, AA, Bartlein, PJ, Christensen, TR & Cramer, W 2003, 'Climate change and Arctic ecosystems: 1. Vegetation changes north of 55 N between the last glacial maximum, mid-Holocene, and present', Journal of Geophysical Research: Atmospheres, vol. 108, no. D19.

Jeppesen, E 2003, 'Climate change: Lakes of the future in mini format', Danish Environment Newsletter, vol. 27, pp. 1. <http://www.mex.dk/uk/vis_nyhed_uk.asp?id=6085&nyhedsbrev_id=877>

Displaying results 324601 to 324650 out of 390544

6493

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen