Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Kiss, EL, Berkó, S, Gácsi, A, Kovács, A, Katona, G, Soós, J, Csányi, E, Gróf, I, Harazin, A, Deli, MA & Budai-Szűcs, M 2019, 'Design and optimization of nanostructured lipid carrier containing dexamethasone for ophthalmic use', Pharmaceutics, vol. 11, no. 12, 679. https://doi.org/10.3390/pharmaceutics11120679

Unnikrishnan, R, Deshmukh, A, Ramesh, S, Babu, SK, Anitha, P & Bhavani, RR 2019, Design and Perception of a Social Robot to Promote Hand Washing among Children in a Rural Indian School. in 2019 28th IEEE International Conference on Robot and Human Interactive Communication, RO-MAN 2019., 8956450, IEEE, 28th IEEE International Conference on Robot and Human Interactive Communication, RO-MAN 2019, New Delhi, India, 14/10/2019. https://doi.org/10.1109/RO-MAN46459.2019.8956450

Soprani, S, Marongiu, F, Christensen, L, Alm, O, Petersen, KD , Ulrich, T & Engelbrecht, K 2019, 'Design and testing of a horizontal rock bed for high temperature thermal energy storage', Applied Energy, vol. 251, 113345. https://doi.org/10.1016/j.apenergy.2019.113345

Valero Moreno, J, Centola, M, Ma, Y, Škugor, M, Yu, Z, Haydell, MW, Keppner, D & Famulok, M 2019, 'Design, assembly, characterization, and operation of double-stranded interlocked DNA nanostructures', Nature Protocols, vol. 14, no. 10, pp. 2818-2855. https://doi.org/10.1038/s41596-019-0198-7

Andersen, P 2019, Design din digitale transformation.

Carlson, DF , Melvad, C , Rysgaard, S , Vesterled, L, Skovby, M, Fürsterling, A & Pedersen, SS, Design files and LabVIEW VIs for the Arctic Research Center Autonomous Boat (ARCAB), 2019, Dataset, Elsevier, Mendeley. https://doi.org/10.17632/287wmj4mzj.1

Carlson, DF, Pasma, J, Jacobsen, M, Hansen, M, Thomsen, S, Lillethorup, J, Tirsgaard, F, Flytkjær, A & Melvad, C, Design files and LabVIEW VIs for the IceDrone, 2019, Dataset, Elsevier. <https://data.mendeley.com/datasets/jx7b5t4f6j/1>

Boer, H & Boer, H 2019, 'Design-for-variety and operational performance: The mediating role of internal, supplier and customer integration', Journal of Manufacturing Technology Management, vol. 30, no. 2, pp. 438-461. https://doi.org/10.1108/JMTM-03-2018-0065

Katibeh, M, Kalantarion, M, Sabbaghi, H, Mousavi, B, Schriver, M, Nikkhah, H, Ahmadieh, H & Kallestrup, P 2019, 'Designing a screening program for prevention of avoidable blindness in Iran through a participatory action approach', Journal of Ophthalmic and Vision Research, vol. 14, no. 1, pp. 52-61. https://doi.org/10.4103/jovr.jovr_43_18

Hammershøj, M 2019, 'Designing biofunctional dairy foods: Matrix structure of dairy products in relation to lipaemia', Dairy Research Conference 2019, Billund, Denmark, 27/03/2019 - 27/03/2019.

Yildiz, E, Melo, M, Møller, C & Bessa, M 2019, Designing Collaborative and Coordinated Virtual Reality Training Integrated with Virtual and Physical Factories. in 2019 International Conference on Graphics and Interaction (ICGI). IEEE, pp. 48-55, 2019 International Conference on Graphics and Interaction, Faro, Portugal, 21/11/2019. https://doi.org/10.1109/ICGI47575.2019.8955033

Tutenges, S & Bøhling, F 2019, 'Designing drunkenness: How pubs, bars and nightclubs increase alcohol sales', International Journal of Drug Policy, vol. 70, pp. 15-21. https://doi.org/10.1016/j.drugpo.2019.04.009

Niemantsverdriet, K, van Essen, H, Pakanen, MA & Eggen, B 2019, 'Designing for Awareness in Interactions with Shared Systems: The DASS Framework', ACM Transactions on Computer-Human Interaction, vol. 26, no. 6, 36. https://doi.org/10.1145/3338845

Hilli, C, Nørgård, RT & Aaen, JH 2019, 'Designing Hybrid Learning Spaces in Higher Education', Dansk Universitetspædagogisk Tidsskrift, vol. 15, no. 27, pp. 66-82. <https://dun-net.dk/media/944279/dut27_hilli_et_al_designing_hybrid_learning.pdf>

Van Mechelen, M 2019, Designing Internet of Toys for and with Children: A Participatory Design Case Study. in G Mascheroni & D Holloway (eds), The Internet of Toys: Practices, Affordances and the Political Economy of Children’s Smart Play. Palgrave Macmillan, Cham, Studies in Childhood and Youth, pp. 181-203. https://doi.org/10.1007/978-3-030-10898-4_9

Hasse, C & Søndergaard, DM (eds) 2019, Designing Robots, Designing Humans. Routledge.

Heiselberg, K 2019, Designing self-tracking devices for vulnerable chronic ill. in DIS 2019 Companion - Companion Publication of the 2019 ACM Designing Interactive Systems Conference. Association for Computing Machinery, pp. 89-92, 2019 ACM Conference on Designing Interactive Systems, DIS 2019, San Diego, United States, 23/06/2019. https://doi.org/10.1145/3301019.3324876

Parsons, CE , Jensen, KL , Roepstorff, A , Fjorback, LO & Linehan, C 2019, 'Designing technology tools to support engagement in mindfulness-based interventions: an analysis of teacher and student experiences', Digital Health, vol. 5. https://doi.org/10.1177/2055207619868550

Hussain, A, Chaniago, YD, Riaz, A & Lee, M 2019, 'Design method for the feasibility and technical evaluation of side-reactor column configurations', Chemical Engineering and Processing - Process Intensification, vol. 144, 107648. https://doi.org/10.1016/j.cep.2019.107648

Jaén, KE, Velázquez, D, Sigala, JC & Lara, AR 2019, 'Design of a microaerobically inducible replicon for high-yield plasmid DNA production', Biotechnology and Bioengineering, vol. 116, no. 10, pp. 2514-2525. https://doi.org/10.1002/bit.27091

Wijsenbeek, M, Bendstrup, E, Valenzuela, C, Henry, MT, Moor, C, Bengus, M, Perjesi, A, Gilberg, F, Kirchgaessler, K-U & Vancheri, C 2019, 'Design of a Study Assessing Disease Behaviour During the Peri-Diagnostic Period in Patients with Interstitial Lung Disease: The STARLINER Study', Advances in Therapy, vol. 36, no. 1, pp. 232-243. https://doi.org/10.1007/s12325-018-0845-3

Lara, AR, Velázquez, D, Penella, I, Islas, F, González-De la Rosa, CH & Sigala, JC 2019, 'Design of a synthetic miniR1 plasmid and its production by engineered Escherichia coli', Bioprocess and Biosystems Engineering, vol. 42, no. 8, pp. 1391-1397. https://doi.org/10.1007/s00449-019-02129-2

Jaspers, SØ, Jakobsen, LM, Gadegaard, CA, Dyreborg, J, Andersen, LPS & Aust, B 2019, 'Design of a tailored and integrated violence prevention program in psychiatric wards and prisons', Work, vol. 62, no. 4, pp. 525-541. https://doi.org/10.3233/WOR-192888

Knudsen, J , Sørensen, KK, Steenfelt, JS & Trankjær , H 2019, 'Design of bored piles in Denmark - a historical perspective', Paper presented at XVII European Conference on Soil Mechanics and Geotechnical Engineering, Reykjavik, Iceland, 01/09/2019 - 06/09/2019. https://doi.org/10.32075/17ECSMGE-2019-0732

Barbero, N, Abbiati, G, Enrico, E, Amato, G, Ott, HR, Mesot, J & Shiroka, T 2019, 'Design Optimization through Thermomechanical Finite-Element Analysis of a Hybrid Piston-Clamped Anvil Cell for Nuclear Magnetic Resonance Experiments', Review of Scientific Instruments, vol. 90, no. 1, 013901. https://doi.org/10.1063/1.5059391

Subrizi, A, del Amo, EM, Korzhikov-Vlakh, V, Tennikova, T, Ruponen, M & Urtti, A 2019, 'Design principles of ocular drug delivery systems: importance of drug payload, release rate, and material properties', Drug Discovery Today, vol. 24, no. 8, pp. 1446-1457. https://doi.org/10.1016/j.drudis.2019.02.001

Reick, P 2019, 'Desire or Displacement? Working-Class Notions of Urban Belonging in Late-Nineteenth-Century Germany', Journal of Urban History, vol. 45, no. 6, pp. 1193-1211. https://doi.org/10.1177/0096144218792503, https://doi.org/10.1177/0096144218792503

Pfattheicher, S, Keller, J & Knezevic, G 2019, 'Destroying things for pleasure: On the relation of sadism and vandalism', Personality and Individual Differences, vol. 140, no. April, pp. 52-56. https://doi.org/10.1016/j.paid.2018.03.049

Nielsen, JC & Stilling Olesen, J 2019, De synlige talenter. in JC Nielsen & JS Olesen (eds), Talentudvikling og elitesport i skolen. Aarhus Universitetsforlag, Aarhus, Asterisk (bogserie), no. 18, pp. 191-214.

Christoffersen, EE 2019, 'Det Åbne Hjerte av Signa', Norsk Shakespeare og Teater-Tidsskrift, vol. 2019, no. 4, pp. 100-105.

Christoffersen, EE 2019, 'Det begynder at blive koldt om fødderne: En livsbekræftende rejse mod døden', Peripeti. <http://www.peripeti.dk/2019/10/31/koldt-om-foedderne/>

Højlund, S & Selmer, B 2019, 'Det brankede: Tanker om grillpølsen', Jordens Folk, vol. 54, no. 2, pp. 69-75.

Mortensen, E & Raja, R 2019, Det danske engagement i Den Arabiske Golf. Carlsbergfondet. <https://www.carlsbergfondet.dk/da/Nyheder/Formidling/Store-Danske-Arkaeologer/Bahrain>

Mortensen, E & Raja, R 2019, Det danske engagement i Palmyra. Carlsbergfondet. <https://www.carlsbergfondet.dk/da/Nyheder/Formidling/Store-Danske-Arkaeologer/Palmyra>

Pade, M 2019, 'Det Danske Institut i Rom: et hovedværk i skandinavisk modernistisk arkitektur', Romhorisont, no. 70, pp. 10-11.

Pedersen, VA (ed.) & Grundtvig, NFS 2019, Det danske Maigilde. (Til Udgiveren.). Grundtvigs Værker. <http://www.xn--grundtvigsvrker-7lb.dk/tekstvisning/18480/0#>

Qvortrup, L 2019, 'Det danske uddannelsessystem er godt,men dyrt', Skolemonitor.

Aagaard, A 2019, 'Det digitale landbrug er et must', Jyllands-Posten.

Bøss, M 2019, Det digitale massemenneske: Menneskeværd i ensomhedens tid. People'sPress, København.

Nørgård, RT 2019, Det digitale (nordiske) universitetsbibliotek som videnskabende ressource og pædagogisk aktør i nordisk universitets-uddannelse (2016-18): Opsamling på resultater fra workshop på Minikonference 19.6.19 som afslutning på Nordplus projektet. <https://library.au.dk/fileadmin/www.bibliotek.au.dk/Blogs/Nordplus_-_opsamling_af_resultater_-_Final.pdf>

Bak Herrie, M 2019, 'Det digitale objekt: æstetik, data og kulturteknik', Aarhus.

Sivitskis, AJ, Lehner, JW, Harrower, MJ, Dumitru, IA, Paulsen, PE, Nathan, S, Viete, DR, Al-Jabri, S, Helwing, B, Wiig, F, Moraetis, D & Pracejus, B 2019, 'Detecting and Mapping Slag Heaps at Ancient Copper Production Sites in Oman', Remote Sensing, vol. 11, no. 3014, 3014. https://doi.org/10.3390/rs11243014

Chen, T-HK, Prishchepov, A, Fensholt, R & Sabel, CE 2019, 'Detecting and monitoring long-term landslides in urbanized areas with nighttime light data and multi-seasonal Landsat imagery across Taiwan from 1998 to 2017', Remote Sensing of Environment, vol. 225, pp. 317-327. https://doi.org/10.1016/j.rse.2019.03.013

Chen, THK , Prishchepov, AV, Fensholt, R & Sabel, CE 2019, 'Detecting and monitoring long-term landslides in urbanized areas with nighttime light data and multi-seasonal Landsat imagery across Taiwan from 1998 to 2017', Remote Sensing of Environment, vol. 225, pp. 317-327. https://doi.org/10.1016/j.rse.2019.03.013

Andersen, LE & Wray, KB 2019, 'Detecting errors that result in retractions', Social Studies of Science, vol. 49, no. 6, pp. 942-954. https://doi.org/10.1177/0306312719872008

Skov, DS , Egholm, DL , Jansen, JD, Sandiford, M & Knudsen, MF 2019, 'Detecting landscape transience with in situ cosmogenic ¹⁴C and ¹⁰Be', Quaternary Geochronology, vol. 54, 101008. https://doi.org/10.1016/j.quageo.2019.101008

Spindola, LM, Santoro, ML, Pan, PM, Ota, VK, Xavier, G, Carvalho, CM, Talarico, F, Sleiman, P, March, M, Pellegrino, R, Brietzke, E, Grassi-Oliveira, R, Mari, JJ, Gadelha, A, Miguel, EC, Rohde, LA, Bressan, RA, Mazzotti, DR, Sato, JR, Salum, GA, Hakonarson, H & Belangero, SI 2019, 'Detecting multiple differentially methylated CpG sites and regions related to dimensional psychopathology in youths', Clinical Epigenetics, vol. 11, no. 1, 146. https://doi.org/10.1186/s13148-019-0740-z

Nissen, P 2019, 'Detecting Talent from the Perspectives of Students, Parents, and Teachers', US-China Education Review B, vol. 9, no. 4, 1, pp. 119-127. https://doi.org/10.17265/2161-6248/2019.04.001

Bhatta, TR, Chamings, A, Vibin, J & Alexandersen, S 2019, 'Detection and characterisation of canine astrovirus, canine parvovirus and canine papillomavirus in puppies using next generation sequencing', Scientific Reports, vol. 9, no. 1, 4602. https://doi.org/10.1038/s41598-019-41045-z

Luque, R, Nowak, G, Pallé, E, Dai, F, Kaminski, A, Nagel, E, Hidalgo, D, Bauer, F, Lafarga, M, Livingston, J, Barragán, O, Hirano, T, Fridlund, M, Gandolfi, D, Justesen, AB , Hjorth, M, Van Eylen, V, Winn, JN, Esposito, M, Morales, JC, Albrecht, S, Alonso, R, Amado, PJ, Beck, P, Caballero, JA, Cabrera, J, Cochran, WD, Csizmadia, S, Deeg, H, Eigmüller, P, Endl, M, Erikson, A, Fukui, A, Grziwa, S, Guenther, EW, Hatzes, AP, Knudstrup, E, Korth, J, Lam, KWF, Lund, MN, Mathur, S, Montañes-Rodríguez, P, Narita, N, Nespral, D, Niraula, P, Pätzold, M, Persson, CM, Prieto-Arranz, J, Quirrenbach, A, Rauer, H, Redfield, S, Reiners, A, Ribas, I & Smith, AMS 2019, 'Detection and characterization of an ultra-dense sub-Neptunian planet orbiting the Sun-like star K2-292', Astronomy and Astrophysics, vol. 623, A114. https://doi.org/10.1051/0004-6361/201834952

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Lise Refstrup Linnebjerg Pedersen