Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Wils, RS, Nielsen, HB, Flachs, EM, Andersen, AJ, Dreyer, LW, Ketzel, M , Brandt, J , Khan, J, Vogel, U, Sejbaek, CS & Hougaard, KS 2025, 'Air pollution and risk of rheumatoid arthritis – A Danish register-based cohort study', Environmental Toxicology and Pharmacology, vol. 120, 104855. https://doi.org/10.1016/j.etap.2025.104855

Montanari, P, Vitali, A & Ferraretto, V 2025, 'Air pollution and subjective wellbeing: exploring the link between PM10 and a composite measure of wellbeing', Population and Environment, vol. 47, no. 4, 38. https://doi.org/10.1007/s11111-025-00504-8

ECHO Cohort Consortium 2025, 'Air Pollution Exposure and Birth Weight in the ECHO Cohort', JAMA network open, vol. 8, no. 12, e2551459. https://doi.org/10.1001/jamanetworkopen.2025.51459

Brustad, N, Wang, T, He, S, Pedersen, CET, Gruzieva, O, Chen, L, Pershagen, G, Ali, M, Kyvsgaard, JN, Pedersen, M, Ketzel, M, Hyöty, H, Agardh, D, Melén, E, Thorsen, J, Stokholm, J, Bønnelykke, K & Chawes, B 2025, 'Air pollution-induced proteomic alterations increase the risk of child respiratory infections', Nature Communications, vol. 16, no. 1, 5930. https://doi.org/10.1038/s41467-025-61392-y

Ye, D, Huang, L & Eriksson, T 2025, 'Air Pollution Induces Aggregate Productivity Growth: Evidence from Chinese Firms through Productivity Decomposition Analysis', Environment, Development and Sustainability, vol. 27, no. 5, pp. 12153-12192. https://doi.org/10.1007/s10668-024-05758-0

Renic, N & Christensen, JG 2025, 'Air Power by Small, Low-Cost Drones: Implications for Future Warfare', The Air Power Journal. <https://theairpowerjournal.com/air-power-by-small-low-cost-drones-implications-for-future-warfare/>

Jensen, SS , Ketzel, M , Khan, J , Valencia, VH , Brandt, J , Christensen, JH , Frohn, LM , Geels, C , Nielsen, OK , Plejdrup, MS & Ellermann, T 2025, 'Air Quality at Your Street 2.0—Air Quality Modelling for All Streets in Denmark', Atmosphere, vol. 16, no. 12, 1346. https://doi.org/10.3390/atmos16121346

Poncin, W, Schrøder, C, Oliveira, A, Herrero, B, Cnockaert, P, Gely, L, Osadnik, C, Reychler, G, Mechlenburg, I & Spinou, A 2025, 'Airway clearance techniques for people with acute exacerbation of COPD: a scoping review', European respiratory review : an official journal of the European Respiratory Society, vol. 34, no. 175, 240191. https://doi.org/10.1183/16000617.0191-2024

Tække, J 2025, 'AI som teknologi: fra organisationsprogrammer til teknologiske beslutningspræmisser', Paper presented at Programmes – Observed with Social Systems Theory, Cambridge, United Kingdom, 09/09/2025 - 12/09/2025 pp. 1-25.

Nooshabadi, J, Butler, C, Jonasson, C , Lauring, J & Nordbäck, E 2025, 'AI, trust, and digital leadership in virtual teams: Reconceptualising workplace relations in the digital era', 2nd International Symposium ‘Digitalisation, Artificial Intelligence, Work and Employment’, Brighton, United Kingdom, 13/10/2025 - 15/10/2025.

Rysgaard, CM 2025, 'A Journey through Persistence, External Memory, Trees, and other Unrelated Problems', PhD, Aarhus University.

Gabs, E, Aalto-Setälä, E, Välisaari, A, Malinen, AM, Jensen, TH, McLaughlin, SH, Passmore, LA & Turtola, M 2025, 'A kinetic ruler controls mRNA poly(A) tail length', Genes & Development, vol. 39, no. 21-22, pp. 1377-1394. https://doi.org/10.1101/gad.352912.125

Østergaard, CES, Bertelsen, T, Lomholt, H, Kofoed, K & Gyldenløve, M 2025, 'Akne', Ugeskrift for Læger, vol. 187, no. 14. https://doi.org/10.61409/V09240660

Armitage, E , Steglich-Petersen, A & Varga, S 2025, 'Akratic Thinking', Philosophical Psychology. https://doi.org/10.1080/09515089.2025.2563069

Cederfeldt, JBM 2025, 'Aksetiden: Status quaestionis', Slagmark: tidsskrift for idéhistorie , vol. 89.

Dalskov, S, Lægsgaard, E, Christensen, BB , Høy, K , Nielsen, MA & Rickers, KW 2025, 'Akut paraspinalt kompartmentsyndrom', Ugeskrift for Læger, vol. 187, no. 11, V10240692, pp. 1-3. https://doi.org/10.61409/V10240692

Agger-Nielsen, HE, Owen, H & Ovesen, T 2025, 'Akut vestibulær svimmelhed', Ugeskrift for Læger, vol. 187, no. 42, V03250240, pp. 1-9. https://doi.org/10.61409/V03250240

Kuang, VH , Stald Skoven, C , Arvin, S , Moberg Fitting, L , Drasbek, KR , Hansen, B , Orlowski, D & Sørensen, JCH 2025, 'A large animal model for focal stroke: Photothrombotic lesion in the cortex of Danish Landrace pigs', Journal of Neuroscience Methods, vol. 418, 110408. https://doi.org/10.1016/j.jneumeth.2025.110408

Hald, M , Nielsen, TS, Bruun, TS, Knage-Drengfeldt, K & Pedersen, TF 2025, 'A large pellet size rather than diet complexity increases piglet creep feed disappearance and feeding activity but not growth performance', Translational Animal Science, vol. 9, txaf114. https://doi.org/10.1093/tas/txaf114

Lieberoth, A, Nielsen, SM, Reynen-de Kat, C, Rotaru, C, Niazyan, L, Parandzem, P, Craig, BJ, Karstadt, OM, Demiscan, D, Paraschiv, A & Gutu, V 2025, 'A large-scale cross-country trial of WHO’s Immune Patrol: toward a game-based digital curriculum for vaccine readiness and global health education', Frontiers in Education, vol. 10, 1472018. https://doi.org/10.3389/feduc.2025.1472018

Christensen, KR & Clasen, M 2025 'A large-scale survey of patterns in the beliefs in science, the supernatural, spirituality, and magic in secular Denmark (preprint).' PsyArXiv. https://doi.org/10.31234/osf.io/pmqzf_v1

Nørgaard, J, Margold, M, Jansen, JD, Szuman, I, Lukyanycheva, M, Andersen, JL , Pedersen, VK , Olsen, J, Corbett, LB, Bierman, PR & Knudsen, MF 2025, 'A late Pleistocene glacial maximum during MIS 3 in the Chersky Mountains, central northeast Siberia', Quaternary Research (United States), vol. 128, pp. 91-101. https://doi.org/10.1017/qua.2025.20

Fuchs, E 2025, 'Albumin-Based Drug Designs for Immunotherapy', PhD, Aarhus University.

Aglago, EK, Ramos, I, Keski-Rahkonen, P, Chatziioannou, C, Freisling, H, Fedirko, V, Gunter, MJ, Dahm, CC , Langmann, F, Bondonno, N, Tjønneland, A, Severi, G, Truong, T, Katzke, V, Kaaks, R, Bergmann, M, Schulze, MB, Masala, G, Pala, V, de Magistris, MS, Di Girolamo, C, Lukic, M, Gram, IT, Bonet, C, Sánchez, M-J, Chirlaque, M-D, Amiano, P, Guevara, M, Vermeulen, R, Manjer, J, Eriksson, L, Key, TJ, Mayen, A-L, Dossus, L, Weiderpass, E, Heath, AK, Ferrari, P & Jenab, M 2025, 'Alcohol and smoking habits in association with hepatocellular carcinoma risk', International Journal of Cancer, vol. 157, no. 4, pp. 644-657. https://doi.org/10.1002/ijc.35401

Danpanichkul, P, Pang, Y, Díaz, LA, White, TM, Sirimangklanurak, S, Auttapracha, T, Suparan, K, Syn, N, Jatupornpakdee, P, Saowapa, S, Ng, CH, Kaewdech, A, Lui, RN, Fallon, MB, Yang, JD, Louvet, A, Noureddin, M, Liangpunsakul, S, Jepsen, P, Lazarus, JV, Arab, JP & Wijarnpreecha, K 2025, 'Alcohol-Attributable Cancer: Update From the Global Burden of Disease 2021 Study', Alimentary Pharmacology and Therapeutics, vol. 62, no. 1, pp. 33-47. https://doi.org/10.1111/apt.70163

GBD 2019 Nordic Alcohol Collaborators 2025, 'Alcohol-attributed disease burden and formal alcohol policies in the Nordic countries (1990-2019): an analysis using the Global Burden of Disease Study 2019', European Journal of Public Health, vol. 35, no. 1, ckae195, pp. 52-59. https://doi.org/10.1093/eurpub/ckae195

Oraii, A, Conen, D, Johnson, LS, McIntyre, WF, Kirabo, F, Balasubramanian, K, Benz, AP, Oldgren, J, Nielsen, JC & Healey, J 2025, 'Alcohol Intake and Cardiovascular Outcomes in Patients With Atrial Fibrillation: RE-LY AF Registry Analysis', Annals of Noninvasive Electrocardiology, vol. 30, no. 4, e70096. https://doi.org/10.1111/anec.70096

Clausen, KK , Sterup, J & Bregnballe, T 2025, 'Alders- og underartssammensætning af skarver reguleret i Danmark', 16 p., Fagligt notat fra DCE – Nationalt Center for Miljø og Energi (2020-...), no. 33. <https://dce.au.dk/fileadmin/dce.au.dk/Udgivelser/Notater_2025/N2025_33.pdf>

Sørensen, SS & Svendsen, M-MM 2025, 'Aldrig før er der blevet skrevet en bog med så mange imperativer: Anmeldelse af Ruben Schachtenhaufen, Michael Bach Ipsen og Mikael Fabrin: Dansk Udtaleordbog. Engelsk undertitel: Contemporary Danish Pronunciation', NyS : Nydanske Sprogstudier, vol. 68, pp. 73-90. https://doi.org/10.7146/nys.v1i68.160532

Andreasen, J 2025, Aldrig mere: Spil om Besættelsestiden 1940-45 : en introduktion. a'jon.

Weise, KF, Madsen, KH, Worbs, T, Knösche, TR, Korshoej, AR & Thielscher, A 2025, 'A leadfield-free optimization framework for transcranially applied electric currents', Computers in Biology and Medicine, vol. 195, 110648. https://doi.org/10.1016/j.compbiomed.2025.110648

Tolstrup, J 2025, Aleksandr Lukasjenko. <https://lex.dk/Aleksandr_Lukasjenko>

Feord, HK, Trautwein-Schult, A, Keuschnig, C, Ostrzinski, A, Peter, EK, Jaeger, C, Lisec, J, Mourot, R, Peters, RS, Çiftçi, O, Tranter, M , Anesio, AM, Becher, D & Benning, LG 2025, 'Algae-dominated metaproteomes uncover cellular adaptations to life on the Greenland Ice Sheet', npj Biofilms and Microbiomes, vol. 11, no. 1, 181. https://doi.org/10.1038/s41522-025-00770-2

Heymann, M 2025, Algorithmen, Politik und wissenschaftliche Standards: Wie die Klimavorhersage die Kultur der Klimawissenschaft veränderte. in U Hashageb & R Seising (eds), Algorithmische Wissenskulturen, Der Einfluss des Computers auf die Wissenschaftsentwicklung. Springer Fachmedien Wiesbaden, Die blaue Stunde der Informatik, pp. 171-189. https://doi.org/10.1007/978-3-658-35560-9

Lippert-Rasmussen, K 2025, 'Algorithmic and Non-Algorithmic Fairness: Should We Revise our View of the Latter Given Our View of the Former?', Law and Philosophy, vol. 44, no. 2, pp. 155-179. https://doi.org/10.1007/s10982-024-09505-4

Schou, JKR 2025, 'Algorithmic Innovation through Persistence, Topology, and Geometry', PhD, Aarhus University.

Eichner, S, Thiele, F & Tewari, R 2025, 'Algorithmic Media: Shifting Agency, Content and Everyday Uses', Journal of Creative Communications, vol. 20, no. 1, pp. 7-10. https://doi.org/10.1177/09732586241291935

Smith, RC & Garnham, I 2025, 'Algorithmic Sites: Exploring notions of AI beyond interactions across Global South communities', Net-IAS Debate: Computational Practices in the "Rest of the World", Aarhus , Denmark, 15/01/2025 - 15/01/2025.

Mungi, NA, Galetti, M, Ratnam, J, Chala, D, Sanín, MJ & Svenning, J-C 2025, 'Alien plants and novel ecosystems in the Greater Tropics', Nature Reviews Biodiversity, vol. 1, pp. 515–531. https://doi.org/10.1038/s44358-025-00068-3

Rydgård, M, Jensen, LS, Schwartzkopff, MV, Pinna, S, Sørensen, P & Bruun, S 2025, 'A life cycle assessment of cover crop ensiling and co-digestion with cattle manure', Nutrient Cycling in Agroecosystems, vol. 130, no. 2. https://doi.org/10.1007/s10705-024-10386-1

Barakat, A, Doncheva, NT, Prado, J, Moll, L, Jensen, JL, Díaz-delCastillo, M, Novak, I, Eijkelkamp, N, Jensen, LJ & Heegaard, A-M 2025, 'A ligand-receptor interactome of the bone tumor microenvironment in multiple myeloma bone pain', Pain, vol. 166, no. 12, 10.1097/j.pain.0000000000003746, pp. 2831-2850. https://doi.org/10.1097/j.pain.0000000000003746

Siebold, N , Günzel-Jensen, F & Korsgaard, S 2025, 'Aligning with a global agenda? How ethical imperatives and collaboration orientation shape SDG adoption in social ventures', International Small Business Journal, vol. 43, no. 8, pp. 863-894. https://doi.org/10.1177/02662426251349643

Almasi, M & Kristensen-McLachlan, RD 2025, Alignment Drift in CEFR-prompted LLMs for Interactive Spanish Tutoring. in E Kochmar, B Alhafni, M Bexte, J Burstein, A Horbach, R Laarmann-Quante, A Tack, V Yaneva & Z Yuan (eds), Proceedings of the 20th Workshop on Innovative Use of NLP for Building Educational Applications (BEA 2025). Association for Computational Linguistics, Vienna, Austria, pp. 70-82. https://doi.org/10.18653/v1/2025.bea-1.6

Fundal, HN, Rambøll, JE & Olsen, K 2025 'Alignment, Exploration, and Novelty in Human-AI Interaction' ArXiv. https://doi.org/10.48550/arXiv.2512.17117

Weeks, A, Van Eylen, V, Huber, D, Kawata, D, Stokholm, A, Børsen-Koch, VA, Pinilla, P, Rørsted, JL , Winther, ML & Berger, T 2025, 'A link between rocky planet composition and stellar age', Monthly Notices of the Royal Astronomical Society, vol. 539, no. 1, pp. 405-421. https://doi.org/10.1093/mnras/staf474

Kragh-Schwarz, M 2025, 'Alkaline Water Electrolysis Through New Eyes.Insights from electrode performance, degradation, and advanced imaging studies', PhD, Aarhus University.

Prummer, F, Weidner, F & Gellersen, H 2025, All But Static: Exploring Dynamic Eye Dominance for Foveated Rendering. in Proceedings - 2025 IEEE Conference on Virtual Reality and 3D User Interfaces Abstracts and Workshops, VRW 2025. IEEE Conference on Virtual Reality and 3D User Interfaces, vol. 2025, pp. 376-380. https://doi.org/10.1109/VRW66409.2025.00087

Fernández de la Cruz, L, Isomura, K, Kuja-Halkola, R, Lichtenstein, P, Larsson, H, Chang, Z, D'Onofrio, BM, Brikell, I, Sidorchuk, A & Mataix-Cols, D 2025, 'All-Cause and Cause-Specific Mortality in Tourette Syndrome and Chronic Tic Disorder', Movement Disorders, vol. 40, no. 2, pp. 335-344. https://doi.org/10.1002/mds.30084

Günal-Köroğlu, D, Karabulut, G, Ozkan, G, Yılmaz, H, Gültekin-Subaşı, B & Capanoglu, E 2025, 'Allergenicity of Alternative Proteins: Reduction Mechanisms and Processing Strategies', Journal of Agricultural and Food Chemistry, vol. 73, no. 13, pp. 7522-7546. https://doi.org/10.1021/acs.jafc.5c00948

Høj, S, Nielsen, FK, Chawes, B, Backer, V, Linneberg, A, Thomsen, SF, Sigsgaard, T & Meteran, H 2025, 'Allergic Rhinitis is Associated with Increased Suicidality: A Systematic Review and Meta-Analysis', Clinical Reviews in Allergy and Immunology, vol. 68, no. 1, 52. https://doi.org/10.1007/s12016-025-09061-2

Displaying results 4651 to 4700 out of 390065

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen