Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Geirnaert, S, Kappel, SL & Kidmose, P 2025, 'A direct comparison of simultaneously recorded scalp, around-ear and in-ear EEG for neural selective auditory attention decoding to speech', Scientific Reports, vol. 15, no. 1, 41655. https://doi.org/10.1038/s41598-025-25576-2

Zoelner, M 2025, 'A discursive approach to the identity work of highly skilled transnational professionals in ambivalent contexts', European Journal of International Management, vol. 25, no. 3/4, pp. 625-644. https://doi.org/10.1504/EJIM.2025.144641

Krieger, J & Hao, YA 2025, 'A Disillusion with the Olympic Fantasy: Olympic Identity Card-Related Controversies from 1972 to 1984', Paper presented at 2025 Conference of the North American Society for Sport History (NASSH) , Gatineau, Canada, 26/05/2025 - 29/05/2025.

Ahern, TP, Collin, LJ, MacLehose, RF, Littenberg, B, Haines, L, Bonnett, M, Asmussen, FB, Chen, J & Lash, TL 2025, 'Adjusting Adjustments: Using External Data to Estimate the Impact of Different Confounder Sets on Published Associations', Epidemiology, vol. 36, no. 3, pp. 381-390. https://doi.org/10.1097/EDE.0000000000001821

Jensen, MB, Balslev, E, Knoop, AS, Tuxen, MK, Højris, I, Jakobsen, EH, Cold, S, Danø, H, Glavicic, V, Kenholm, J & Ejlertsen, B 2025, 'Adjuvant Docetaxel and Cyclophosphamide with or Without Epirubicin for Early Breast Cancer: Final Analysis of the Randomized DBCG 07-READ Trial', Journal of Clinical Oncology, vol. 43, no. 4, pp. 373-380. https://doi.org/10.1200/JCO.24.00836

Galsky, MD, Witjes, JA, Gschwend, JE, Milowsky, MI, Schenker, M, Valderrama, BP, Tomita, Y, Bamias, A, Lebret, T, Shariat, SF, Park, SH, Agerbaek, M, Jha, G, Stenner, F, Ye, D, Giudici, F, Dutta, S, Askelson, M, Nasroulah, F, Zhang, J, Brophy, L & Bajorin, DF 2025, 'Adjuvant Nivolumab in High-Risk Muscle-Invasive Urothelial Carcinoma: Expanded Efficacy From CheckMate 274', Journal of Clinical Oncology, vol. 43, no. 1, pp. 15-21. https://doi.org/10.1200/JCO.24.00340

Galsky, MD, Bajorin, DF, Tomita, Y, Ye, D, Agerbaek, M, Enting, D, Peer, A, Milowsky, M, Kobayashi, K, Grimm, MO, Stenner, F, David, JM, Li, J, Chasalow, SD, Nasroulah, F, Apfel, A, Ünsal-Kaçmaz, K & Necchi, A 2025, 'Adjuvant nivolumab in muscle-invasive urothelial carcinoma: exploratory biomarker analysis of the randomized phase 3 CheckMate 274 trial', Nature Medicine, vol. 31, no. 9, pp. 3062-3073. https://doi.org/10.1038/s41591-025-03802-8

Plougmann Gislinge, JI, Rubeck Petersen, K, Borgquist, S & Ravn, P 2025, 'Adjuvant treatment with tamoxifen for estrogen receptor-positive breast cancer and gynecological risks in premenopausal and perimenopausal women–a systematic review', Climacteric, vol. 28, no. 6, pp. 657-665. https://doi.org/10.1080/13697137.2025.2509839

Zhemchuzhnikov, MK, Versluijs, TSL, Bom, RA, Blok, R, van Bleijswijk, JDL, Reneerkens, J & van Gils, JA 2025, 'A DNA-based dietary study of shorebird chicks in Greenland', Wader Study, vol. 131, no. 3, pp. 226-230. https://doi.org/10.18194/ws.00360

Dyrmann, K 2025, 'A Doctor for the Crown Princess: child mortality and women’s political agency at the Danish court, 1784–1797', The History of the Family, vol. 30, no. 1, pp. 88-107. https://doi.org/10.1080/1081602X.2024.2379808

Ballin, M, Ekblom, Ö, Nordström, A, Ahlqvist, VH & Nordström, P 2025 'Adolescent Cardiorespiratory Fitness and Future Risk of Depression and Antidepressants: A Nationwide Cohort Study with Sibling Comparators' medRxiv, pp. 2025.02.07.25321835. https://doi.org/10.1101/2025.02.07.25321835

Ballin, M, Berglind, D, Henriksson, P, Neovius, M, Nordström, A, Ortega, FB, Sillanpää, E, Nordström, P & Ahlqvist, VH 2025, 'Adolescent cardiorespiratory fitness and risk of cancer in late adulthood: A nationwide sibling-controlled cohort study in Sweden', PLOS Medicine, vol. 22, no. 5 May, e1004597. https://doi.org/10.1371/journal.pmed.1004597

Winding, TN, Bøjstrup, J & Christensen, S 2025, 'Adolescent friendships and their impact on self-rated health in early adulthood. A prospective cohort study', Scandinavian Journal of Public Health, vol. 53, no. 8, pp. 881-888. https://doi.org/10.1177/14034948251337336

Eriksson, H, Herraiz-Adillo, Á, Wennberg, P, Daka, B, Esteban-Cornejo, I, Lenander, C, Berglind, D, Müssener, U, Fang, F, Östgren, CJ, Rådholm, K, Henriksson, P & Ahlqvist, VH 2025 'Adolescent Intelligence and Imaging-Based Atherosclerosis in middle age: A Population Study of Swedish Men' medRxiv. https://doi.org/10.1101/2025.10.03.25337218

Damkjaer, MS & Soerensen, ASM 2026, Adolescents Navigating Sharenting: Autonomy, Intimacy, and Care in the Mediatized Family. in GF Lendvai, A Vizcaíno‑Verdú, A Velicu & A Koltay (eds), Transdisciplinary Perspectives on Sharenting: Parenting, Privacy, and Consent. Routledge, Routledge advances in sociology.

Hansen, ER, Kruckow, S, Ewing, SWF, Nordentoft, M, Thomsen, KR & Tolstrup, JS 2025, 'Adolescent substance use patterns and subsequent risk of mental and behavioural disorders, substance use, and suicidal behaviour: a cohort study', The Lancet Public Health, vol. 10, no. 7, pp. e578-e587. https://doi.org/10.1016/S2468-2667(25)00115-X

Zauro, B, Yuksel, D, Nio, M, Wingrove, P, Beach, D & Hultgren, AK 2025, 'Adopting social process tracing in applied linguistics: Lessons from an English-medium Instruction policy case study', Applied Linguistics. https://doi.org/10.1093/applin/amaf066

Wilson, RE, Sonsthagen, SA, Walsh, AJ & Fox, AD 2025, 'Adoption of non-related goslings and intergenerational family cohesion among Greenland White-fronted Geese (Anser albifrons flavirostris)', Ibis, vol. 167, no. 4, pp. 1080-1088. https://doi.org/10.1111/ibi.13427

Sonne, T , Sveistrup Jensen, T , Kingo, OS , Berntsen, D & Krøjgaard, P 2025, 'A double case study on spontaneous memory development in two young girls across six months', SRCD 2025 Biennial meeting, 01/05/2025 - 03/05/2025.

Kolínský, P, Meier, T, Agius, MR, Bijedić, A, Bokelmann, G, Borleanu, F, Brnović, D, Cambaz, MD, Cammarano, F, Čarman, M, Cauzzi, C, Chernih, D, Csicsay, K, Amulić, SC, Czuba, W, Diaz, J, Dimitrova, L, Dushi, E, Evangelidis, CP, Faccenna, C, Farfuliak, L, Friederich, W, Georgieva, G, Horn, N, Ivančić, I, Jia, Y, Kaviris, G, Kovács, IJ, Lebedev, S, Le Breton, E, Lukešová, R, Mazur, S, van der Meijde, M, Molinari, I, Mustafa, S, Nagel, T , Nielsen, SB, Obermann, A, Papazachos, C, Parolai, S, Paul, A, Piromallo, C, Plicka, V, Rietbrock, A, Rondenay, S, Rossi, G, Rümpker, G, Schiffer, C, Schlömer, A, Sigloch, K, Silvennoinen, H, Sokos, E, Špaček, P, Stipčević, J, Tallarico, A, Tiira, T, Tilmann, F, Valčić, D, Wassermann, J, Wesztergom, V, Xhahysa, A & Čarman, M 2025, 'AdriaArray – a Passive Seismic Experiment to Study Structure, Geodynamics and Geohazards of the Adriatic Plate', Annals of Geophysics, vol. 68, no. 5 Special Issue, DM555. https://doi.org/10.4401/ag-9284

Wierød Borčak, L 2025, Adskilt af tid og rum, men forenet i sang: Fællessang og medier. in Når vi synger: En antologi om sangens betydning. Sangens hus, Herning, pp. 185-198.

Henderson, Z, Cole, J, Thomas, AG, Jones, RG, Wagstaffe, M, Avila, J, Asensio, MC, Li, Z & Syres, KL 2025, 'Adsorption and Thermal Stability of Ionic Liquid Multilayers on ZnO Surfaces', Surfaces, vol. 8, no. 2, 29. https://doi.org/10.3390/surfaces8020029

Şahin, M, Arslan, Y, Atasoy, M & Sillanpää, M 2025, 'Adsorption Performance of Zn(II)-Based Coordination Polymer (ZnMOF) Reinforced Magnetic Activated Biochar (CmBC-Fe₃O₄@ZnMOF) Hybrid Composites', Water Environment Research, vol. 97, no. 6, e70113. https://doi.org/10.1002/wer.70113

Suleman, S, Huang, Y, Jensen, RT, Poggi, AI, Christensen, SB, Fraulund, MM, Anderson, LV, Stinson, SE, Fonvig, CE, Pedersen, O, Holm, JC, Hansen, T & Grarup, N 2025, 'Adult-based Genetic Risk Scores for Insulin Resistance Associate with Cardiometabolic Traits in Children and Adolescents', Journal of Clinical Endocrinology and Metabolism, vol. 110, no. 9, pp. 2645-2654. https://doi.org/10.1210/clinem/dgae882

Hald, C, Raunkiær, M, Nielsen, MK , Dreyer, P & Ludvigsen, MS 2025, 'Advance Care Planning During Healthcare Transitions for Community-Dwelling Older Adults in Their End of Life: A Scoping Review', Journal of Clinical Nursing. https://doi.org/10.1111/jocn.70185

Sannerum, KM, Buur, LE, Lykke, C, Nissen, TN, Nielsen, MK, Larsen, SHK & Neergaard, MA 2025, 'Advance care planning til børn og unge med livstruende eller livsbegrænsende sygdom', Ugeskrift for Læger, vol. 187, no. 48. https://doi.org/10.61409/V05250390

Elgaard, CDB, Laurberg, TB , Agnholt, J , Loft, AG & Hjuler, KF 2025, 'Advanced Combination Therapy with Tofacitinib and Biologic Agents for Psoriasis and Psoriatic Arthritis: A Case Series', Case Reports in Dermatology, vol. 17, no. 1, pp. 427-437. https://doi.org/10.1159/000547724

Oshnoei, S, Mahboubi-Moghaddam, E & Khooban, MH 2025, 'Advanced Defense Strategy for Cyber-Resilient Frequency Control in Real Power Grids', IEEE Transactions on Automation Science and Engineering, vol. 22, pp. 19366-19376. https://doi.org/10.1109/TASE.2025.3594891

Cavazza, A, Molina-Estévez, FJ, Reyes, ÁP, Ronco, V, Naseem, A, Malenšek, Š, Pečan, P, Santini, A, Heredia, P, Aguilar-González, A, Boulaiz, H, Ni, Q, Cortijo-Gutierrez, M, Pavlovic, K, Herrera, I, de la Cerda, B, Garcia-Tenorio, EM, Richard, E, Granados-Principal, S, López-Márquez, A, Köber, M, Stojanovic, M, Vidaković, M, Santos-Garcia, I, Blázquez, L, Haughton, E, Yan, D, Sánchez-Martín, RM, Mazini, L, Aseguinolaza, GG, Miccio, A, Rio, P, Desviat, LR, Gonçalves, MAFV, Peng, L, Jiménez-Mallebrera, C, Molina, FM, Gupta, D, Lainšček, D, Luo, Y & Benabdellah, K 2025, 'Advanced delivery systems for gene editing: A comprehensive review from the GenE-HumDi COST Action Working Group', Molecular Therapy Nucleic Acids, vol. 36, no. 1, 102457. https://doi.org/10.1016/j.omtn.2025.102457

Bektassov, M , Thomsen, LD, Baimatova, N & Glasius, M 2025, 'Advanced determination of biogenic volatile organic compounds in environmental water using dynamic headspace extraction', Microchemical Journal, vol. 214, 113989. https://doi.org/10.1016/j.microc.2025.113989

Drennan, IR, Berg, KM, Böttiger, BW, Chia, YW, Couper, K, Crowley, C, D'Arrigo, S, Deakin, CD, Fernando, SM, Garg, R, Granfeldt, A, Grunau, B, Hirsch, KG, Holmberg, MJ, Kudenchuk, PJ, Lavonas, EJ, Leong, CK-L, Vlok, N, Morley, PT, Moskowitz, A, Neumar, RW, Nicholson, TC, Nikolaou, NI, Nolan, JP, O'Neil, BJ, Ohshimo, S, Parr, M, Pocock, H, Sandroni, C, Scquizzato, T, Soar, J, Welsford, M, Zelop, CM, Skrifvars, MB & Advanced Life Support Task Force Collaborators 2025, 'Advanced Life Support: 2025 International Liaison Committee on Resuscitation Consensus on Science With Treatment Recommendations', Resuscitation, vol. 215 , no. Suppl 2, 110806. https://doi.org/10.1016/j.resuscitation.2025.110806

Advanced Life Support Task Force Collaborators 2025, 'Advanced Life Support: 2025 International Liaison Committee on Resuscitation Consensus on Science With Treatment Recommendations', Circulation, vol. 152, no. 16_suppl_1, pp. S72-S115. https://doi.org/10.1161/CIR.0000000000001360

Jha, AK, Jha, AK, Airao, J & Khanna, N 2025, Advanced Machine Learning for Predicting Tool Wear and Tool Life: A Comprehensive Analysis Using Classification and Regression Techniques. in Recent Trends in Material Processing, Characterization and Applications . Springer, Springer Proceedings in Materials, vol. 73, pp. 405-422. https://doi.org/10.1007/978-981-96-5856-5_31

González, XB 2025, 'Advanced Thermoplasmonic Nanostructures for Dynamic Control of Solar Heating in Smart Windows', PhD, Aarhus University.

Fakhar, A, Canatoy, RC, Galgo, SJC, Rafique, M & Sarfraz, R 2025, 'Advancements in modified biochar production techniques and soil application: a critical review', Fuel, vol. 400, 135745. https://doi.org/10.1016/j.fuel.2025.135745

Migliorini, M, Doll, U, Lawson, NJ, Melnikov, SM, Steinbock, J, Dues, M, Zachos, PK, Röhle, I & MacManus, DG 2025, 'Advancements on the use of Filtered Rayleigh Scattering (FRS) with Machine learning methods for flow distortion in Aero-Engine intakes', Experimental Thermal and Fluid Science, vol. 160, 111325, pp. 111325. https://doi.org/10.1016/j.expthermflusci.2024.111325

Norlyk, AM 2025, 'Advances in Continuous-Time Modelling: Integer-Valued and Graphical Approaches', PhD, Aarhus University, Aarhus.

Anesio, AM, Barker, JD & Bröder, L 2025, 'Advances in Glacier Biogeochemistry: A Global Survey of Dissolved Organic Matter in Glacial Meltwater', Global Biogeochemical Cycles, vol. 39, no. 10, e2025GB008595. https://doi.org/10.1029/2025GB008595

Kaussler, C 2025, 'Advances in Mechanistic Understanding of Transition Metal-Catalyzed Reactions and CO2 Sorbent Synthesis from Nitrogen-Rich Waste Polymers', PhD, Aarhus University.

Dal Corso, M, Pető, Á, Vrydaghs, L, Albert, RM, Kirleis, W, Polo-Diaz, A & Out, WA 2025, 'Advances in phytolith research in archaeology and paleoecology: developments and applications', Vegetation History and Archaeobotany, vol. 34, no. 2, pp. 153–158. https://doi.org/10.1007/s00334-025-01035-w

Cardoso, LM, Rolighed, L, Amrein, K, Pilz, S, Underbjerg, L, Pretorius, M, Cetani, F, Zahn, A, Almquist, M, Makay, O, Marcocci, C, Rejnmark, L, Siggelkow, H, Tsourdi, E, Kamenický, P & Bollerslev, J 2025, 'Advances in the clinical management of parathyroid disorders: report from the 2024 workshop by the ESE educational program on parathyroid disorders', European Journal of Endocrinology, vol. 193, no. 6, pp. R65-R88. https://doi.org/10.1093/ejendo/lvaf204

Anand, R 2025, Advancing a global perspective on board governance: Emerging Insights and Challenges Across Nations. in Advancing a global perspective on board governance: Emerging Insights and Challenges Across Nations. Academy of Management. https://doi.org/10.5465/AMPROC.2025.18306symposium

Fiorini, A, Abalos, D, Capra, F, Mortella, G, Lommi, M, Franceschi, N, Ardenti, F & Tabaglio, V 2025, 'Advancing agroecosystem management through strategic cover crop selection: The role of species identity and community composition for productivity and climate change mitigation', Field Crops Research, vol. 328, 109942. https://doi.org/10.1016/j.fcr.2025.109942

Verholt, AB, Foss, CH, Hinrichsen, LM, Madsen, LH, Odderskov, CSN, Andersen, LH & Lietzen, LW 2025, 'Advancing a patient-centered and holistic approach for older patients with frailty in a vascular surgical department: a quality improvement initiative', European Geriatric Medicine. https://doi.org/10.1007/s41999-025-01355-0

Wang, M, Liu, H, Huang, J, Cai, T, Xu, ZP & Zhang, L 2025, 'Advancing cancer gene therapy: the emerging role of nanoparticle delivery systems', Journal of Nanobiotechnology, vol. 23, no. 1, 362. https://doi.org/10.1186/s12951-025-03433-8

Andersen, L & Birkbak, NJ 2025, 'Advancing circulating tumor DNA detection: Using whole-genome sequencing to power minimal residual disease monitoring in breast cancer', Annals of Oncology, vol. 36, no. 6, pp. 609-611. https://doi.org/10.1016/j.annonc.2025.03.008

Appel, CW, Rauff Denby, K, Fynne, L & Vedsted, P 2025, 'Advancing constipation care through technologies - the development of a digital patient education programme', International Journal of Integrated Care.

Jensen, LK, Bjarnsholt, T, Gottlieb, H & Bue, M 2025, 'Advancing cross-disciplinarity in bone and joint infection science using the COMBINE approach: an example from Denmark', Journal of Bone and Joint Infection, vol. 10, no. 1. https://doi.org/10.5194/jbji-10-1-2025

Fabian-Jessing, BK 2025, 'Advancing experimental ocular gene therapy: From bedside to bench, and back again', PhD, Aarhus University.

Prummer, F, Weidner, F & Gellersen, H 2025, Advancing Eye Dominance Testing: Comparing Traditional Methods with an In-HMD Approach for AR/VR Applications. in Proceedings - 2025 IEEE Conference on Virtual Reality and 3D User Interfaces Abstracts and Workshops, VRW 2025. IEEE Conference on Virtual Reality and 3D User Interfaces, vol. 2025, pp. 112-116. https://doi.org/10.1109/VRW66409.2025.00030

Displaying results 4451 to 4500 out of 390065

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen