Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Sørensen, KI, Pellerin, L & Auken, E 2003, 'An auger tool to estimate hydraulic conductivity using a resistivity analogy', Paper presented at 16th Geophysical Conference and Exhibition of the Australian Society of Exploration Geophysicists (ASEG), Adelaide, 17/12/2010.

Larsen, M , Bjarkam, C , Stoltenberg, M , Sørensen, JCH & Danscher, G 2003, 'An autometallographic technique for myelin staining in formaldehyde-fixed tissue', Histology and Histopathology, no. 18(4), pp. 1125-1130.

Stokholm, A 2003, 'Anbragte børn og døgninstitutionskultur', Anbragt på døgninstitution, no. 3, pp. 1-25.

Kristensen, OS 2003, Anbringelse uden for eget hjem. Forlaget PUC, Viborg.

Thomsen, P, Kjeldsen, AM, Sørensen, JT & Houe, H 2003, 'Andelen af aflivede malkekøer stiger', KvaegInfo, pp. 1150-1150.

Isager, H 2003, Anden lovgivning om skærpet erstatningsansvar. in Lærebog i erstatningsret (s.m. Bo von Eyben). 5. edn, Djøf Forlag, København, pp. 205-213.

Thomsen, HJ 2003, 'Åndens liv i det 20. århundrede - Hannah Arendt og Privacy', Slagmark: tidsskrift for idéhistorie , no. 37, pp. 79-105.

Meyer, MKK 2003, Åndernes fremkaldelse - spiritisme og fotografi. in M Buchardt & PR Bövadt (eds), Den gamle nyreligiøsitet. Vestens gamle kulturarv. Forlaget Anis, Frederiksberg, pp. 97 - 116.

Feldt, L 2003, 'Ånder, spøgelser og andre "umulige" ting: Om kognitiv religionsforskning', Kristeligt Dagblad.

Lund, K & Pedersen, MS 2003, 'Andetsproget på arbejde', Sprogforum, vol. 25 februar 2003, no. 25. <http://inet.dpb.dpu.dk/infodok/sprogforum/udgiv.html>

Rasmussen, LH & Just, SN 2003, 'Andrea Ott og Kirstyn English(eds): Handbook on European Enlargement. A Commentary on the Enlargement Process', Politica - Tidsskrift for politisk videnskab, vol. 35, no. nr. 2, pp. 220-221.

Bentzen, JB 2003 'An empirical analysis of gasoline price convergence for 20 OECD countries'.

Kirsopp, C, Mendes, E, Premraj, R & Shepperd, M 2003, An Empirical Analysis of Linear Adaptation Techniques for Case-Based Prediction. in K D. Ashley & D G. Bridge (eds), Case-Based Reasoning Research and Development: 5th International Conference on Case-Based Reasoning, ICCBR 2003, Trondheim, Norway, June 23-26, 2003, Proceedings. Springer Berlin, Heidelberg, Lecture Notes in Computer Science, vol. 2689, pp. 246-260, 5th International Conference on Case-Based Reasoning, Trondheim, Norway, 23/06/2003. https://doi.org/10.1007/3-540-45006-8_20

Christiansen, C , Engsted, T , Jakobsen, S & Tanggaard, C 2003 'An Empirical Study of the Term Structure of Interest Rates in Denmark, 1993 - 2002'.

Nielsen, OS, Reichardt, P, Pink, D, Lindegaard, J, Daugaard, S, Hermans, C, van Glabbeke, M & Judson, I 2003, 'An EORTC phase 1 study to determine the safety of combined caelyx and ifosfamide in previously untreated adult patients with advanced or metastatic soft tissues sarcomas.'.

Fertin, KJ , Jørgensen, LN , Nielsen, BJ , Paaske, K & Jensen, PE 2003, Anerkendelse af fungicider, insekticider, herbicider og vækstreguleringsmidler i 2002: Landbrugsafgrøder og havebrugsafgrøder. in 20. Danske Planteværnskonference: Status og fremtid. Danmarks JordbrugsForskning, DJF rapport, markbrug, no. 88, pp. 5-15, Danske Planteværnskonference, Denmark, 18/12/2010.

Olsen, AK 2003, 'Anesthesia and analgesia of pigs', ScandLAS Symposium, Lahti, Finland, 17/12/2010.

Heide-Jørgensen, MP, Richard, P, Dietz, R, Laidre, KL, Orr, J & Schmidt, HC 2003, 'An estimate of the fraction of belugas (Delphinapterus leucas) in the Canadian high Arctic that winter in West Greenland', Polar Biology, vol. 26, pp. 318-326.

Klærke, A & Paaske, W 2003, 'Aneurysmer', Månedskrift for Praktisk Lægegerning, no. 11, pp. 1437-1451.

Pedersen, PJ, Vasta, E, Ley, D & Solomos, J 2003, An evaluation of Swedish international migration and ethnic relations: (IMER) Research 1995-2002. FAS, Stockholm.

Madsen, JN, Rassmussen, KD, Schauser, L, Pedersen, CNS, Hein, J & Schierup, MH 2003 'An evaluation of the efficiency of Bayesian multipoint LD mapping'.

Bor, IP , Hindkjær, JJ , Kølvraa, S & Ingerslev, HJ 2003, 'A new approach for screening for Y microdeletions: capillary electrophoresis combined with fluorescent multiplex PCR', Journal of Assisted Reproduction and Genetics, vol. 20, no. 1, pp. 46-51.

Boisen, S 2003, A new concept for feed evaluation based on standardised digestible amino acids and potential physiological energy of nutrient fractions in pig feeds. in Souffrant, W.-B. (Ed.) Progress in Research on Energy and Protein Metabolism and Nutrition. 109. pp. 129-132, A new concept for feed evaluation based on standardised digestible amino acids and potential physiological energy of nutrient fractions in pig feeds, 18/12/2010.

Lauridsen, C & Jensen, SK 2003, A new concept on vitamin supplementation to sows and their progeny. in DSM European Swine Conference Tour, November 2003. Abstract.. A new concept on vitamin supplementation to sows and their progeny, 18/12/2010.

Belter, K, Engsted, T & Tanggaard, C 2003 'A New Daily Dividend-Adjusted Index for the Danish Stock Market, 1985-2002: Construction, Statistical Properties, and Return Predictability'.

Sørensen, JN 2003, 'A new growing system for tomatoes', DARCOF enews, no. 2. <http://www.darcof.dk/enews/jun03/tomato.html>

Johansen, P, Fontaine, AA, Deutsch, S, Manning, KB, Nygaard, H & Tarbell, JM 2003, 'A new method for evaluation of cavitation near mechanical heart valves', Journal of Biomechanical Engineering, vol. 125, no. 5, pp. 663-670.

Andersen, T, Jensen, AS, Lalla, M, Jørgensen, CS & Jørgensen, TM 2003, 'A new method to correlate histology with biomechanical properties in urethral tissue: An in-vitro study using light microscopy and scanning acoustic microscopy', APMIS, vol. 111, no. Suppl 109, pp. 35-38.

Danvy, O 2003, A New One-Pass Transformation into Monadic Normal Form. in G Hedin (ed.), Compiler Construction: 12th International Conference, CC 2003 Held as Part of the Joint European Conferences on Theory and Practice of Software, ETAPS 2003 Warsaw, Poland, April 7–11, 2003 Proceedings. Springer, Lecture Notes in Computer Science, vol. 2622, pp. 77-89, 12th International Conference on Compiler Construction (CC 2003), Warsaw, Poland, 07/04/2003. https://doi.org/10.1007/3-540-36579-6_6

Larsen, K & Larsen, SS 2003, 'A new species of Spatholirion (Commelinaceae) from Thailand and further notes on S. ornatum', Thai Forest Bulletin (BOT.), vol. 31, pp. 39-43.

Banka, R & Barfod, A 2003, 'A new spectacular species of Licuala (Arecaceae: Coryphoideae) from New Guinea', Kew Bulletin, vol. 58, no. 4, pp. 73-75.

Uggerhøj, UI 2003, An experimental foundation for electromagnetic shower formation in the geomagnetic field. in Nucl.Phys.B (Proc. Suppl.). pp. 357-363, International Symposium of Very High Energy Cosmic Ray Interactions, CERN, 17/12/2010.

Tjørnehøj, K, Uttenthal, A, Viuff, B, Larsen, LE, Røntved, CM & Rønsholt, L 2003, 'An experimental infection model for reproduction of calf pneumonia with bovine respiratory syncytial virus (BRSV) based on one combined exposure of calves', Res. Vet. Sci., vol. 74, pp. 55-65.

Andersen, HL, Duch, BU, Nielsen, JB, Jørgensen, B & Ledet, T 2003, 'An experimental model for stricture studies in the anterior urethra of the male rabbit', Urol Res, vol. 31, pp. 363-367.

Hvistendahl, GM 2003, 'An explorative study of the pharmacokinetics and effects of dDAVP on renal water and solute excretion', Århus.

Damgård, IB & Frandsen, GS 2003, An Extended Quadratic Frobenius Primality Test with Average and Worst Case Error Estimates. in A Lingas & BJ Nilsson (eds), Fundamentals of Computation Theory. Springer, Lecture Notes in Computer Science, vol. 2751, pp. 118-131, International Symposium on Fundamentals of Computation Theory, Malmö, Sweden, 12/08/2003. https://doi.org/10.1007/978-3-540-45077-1_12

Randsbaek, F, Lund, S, Kimose, HH, Grove, E, Bøtker, HE, Schmitz, O & Nielsen, TT 2003, 'Angiotensin II inhibition increases cellular glucose transport during reperfusion but not ischemia in pig hearts', Scand Cardiovascular Journal, vol. 37, no. 4, pp. 205-210.

Randsbaek, F, Lund, S, Kimose, HH, Grove, E, Botker, HE, Schmitz, O & Nielsen, TT 2003, 'Angiotensin II inhibition increases cellular glucose transport during reperfusion but not ischemia in pig hearts', Scandinavian Cardiovascular Journal, vol. 37, pp. 205-210.

Randsbæk, F, Lund, S, Kimose, H-H , Grove, E , Bøtker, HE , Schmitz, O & Nielsen, TT 2003, 'Angiotensin II inhibition increases cellular glucose transport during reperfusion but not ischemia in pig hearts', Scandinavian Cardiovascular Journal, vol. 37, no. 4, pp. 205-210.

Randsbaek, F, Lund, S, Kimose, HH , Grove, E , Bøtker, HE , Schmitz, O & Nielsen, TT 2003, 'Angiotensin II inhibition increases cellular glucose transport during reperfusion but not ischemia in pig hearts', Scandinavian Cardiovascular Journal, vol. 37, no. 4, pp. 205-10. https://doi.org/10.1080/14017430310002040

Rosenberg, R 2003, 'Angst og angsttilstande', Læge Helse, vol. 7, pp. 4-5.

Jaunsen, AP, Andersen, MI, Hjorth, J, Fynbo, JPU, Holland, ST, Thomsen, B, Gorosabel, J, Schaefer, BE, Björnsson, G, Natarajan, P & Tanvir, NR 2003, 'An HST study of three very faint GRB host galaxies', Astronomy and Astrophysics, no. 402, pp. 125-132.

Rasmussen, MD & Skjøth, F 2003, Animal health of herds converting to automatic milking. in Book of Abstracts of the 54th Annual Meeting of the European Association for Animal Production. pp. 302-302, Annual Meeting of the European Association for Animal Production, Rome, Italy, 18/12/2010.

Smidt, K , Hansen, LL, Søgaard, TMM, Petersen, LK , Knudsen, UB & Martensen, PM 2003, 'A nine-nucleotide deletion and splice variation in the coding region of the interferon induced ISG12 gene', BBA General Subjects, vol. 1636, no. 3, pp. 227-234. https://doi.org/10.1016/S0925-4439(03)00087-5

Smidt, KCJ , Hansen, LL, Søgaard, TMM, Petersen, LK , Knudsen, UB & Martensen, PM 2003, 'A nine-nucleotide deletion and splice variation in the coding region of the interferon induced ISG12 gene', B B A - Molecular Basis of Disease, vol. 1638, no. 3, pp. 227-34.

Smidt, KCJ, Hansen, LL, Søgaard, TMM, Petersen, LK, Knudsen, UB & Martensen, PM 2003, 'A nine nucleotide deletion and splice variation of the coding region of the interferon induced ISG12 gene', B B A - Molecular Basis of Disease, vol. 1638, pp. 227-234.

Raskin, R 2003, 'An Interview with John Lawlor', Nua: Studies in Contemporary Irish Writing, vol. 4, no. 1-2, pp. 147-157.

Raskin, R 2003, 'An Interview with Mitko Panov on "With Raised Hands"', P.O.V., no. 15, pp. 9-18. <http://imv.au.dk/publikationer/pov/Issue_15/section_1/artc3A.html>

Raskin, R 2003, 'An Interview with Oren Stern on "Funeral at Parc de France"', P.O.V., no. 15, pp. 100-103. <http://imv.au.dk/publikationer/pov/Issue_15/section_3/artc2A.html>

Raskin, R 2003, 'An Interview with Tom Remlov on "Derailment"', P.O.V., no. 15, pp. 54-56. <http://imv.au.dk/publikationer/pov/Issue_15/section_2/artc4A.html>

Displaying results 323901 to 323950 out of 390805