Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Nørskov-Lauritsen, N & Ridderberg, W 2019, Approaches to the Identification of Aerobic Gram-Negative Bacteria. in KC Carroll & MA Pfaller (eds), Manual of Clinical Microbiology. 12. edn, vol. Vol. 1, ASM Press, Washington, DC, pp. 630-639.

Sabatini, S, Bergerbrant, S, Brandt, L, Margaryan, A & Allentoft, ME 2019, 'Approaching sheep herds origins and the emergence of the wool economy in continental Europe during the Bronze Age', Archaeological and Anthropological Sciences, vol. 11, no. 9, pp. 4909-4925. https://doi.org/10.1007/s12520-019-00856-x

Rudbeck-Resdal, J , Nielsen, JC, Bundgaard, H & Jensen, HK 2019, 'Appropriate use of genetics in a young patient with atrioventricular block and family history of sudden cardiac death', HeartRhythm Case Reports, vol. 5, no. 3, pp. 169-172. https://doi.org/10.1016/j.hrcr.2018.12.004

Schmitz, G , Artiukhin, DG & Christiansen, O 2019, 'Approximate high mode coupling potentials using Gaussian process regression and adaptive density guided sampling', Journal of Chemical Physics, vol. 150, no. 13, 131102. https://doi.org/10.1063/1.5092228

Andersen, M, Panosetti, C & Reuter, K 2019, 'A practical guide to surface kinetic Monte Carlo simulations', Frontiers in Chemistry, vol. 7, no. APR, 202. https://doi.org/10.3389/fchem.2019.00202

Bjørnshave, A, Holst, JJ & Hermansen, K 2019, 'A pre-meal of whey proteins induces differential effects on glucose and lipid metabolism in subjects with the metabolic syndrome: a randomised cross-over trial', European Journal of Nutrition, vol. 58, no. 2, pp. 755-764. https://doi.org/10.1007/s00394-018-1684-3

Mansour, R, Zhu, J, Edgren, M & Barsoum, Z 2019, 'A probabilistic model of weld penetration depth based on process parameters.', International Journal of Advanced Manufacturing Technology, vol. 105, pp. 499–514. https://doi.org/10.1007/s00170-019-04110-5

Asmuß, B & Aggerholm, HK 2019, 'A process perspective on collective leadership', Paper presented at 1th International Process Symposium, Greece, 20/06/2019 - 22/06/2019.

Khazaee, M, Rezaniakolaei, A & Rosendahl, L 2019, A proorf for looking differently into damping modeling in piezoelectric energy harvesting systems. in Proceedings of the 26th International Congress on Sound and Vibration, ICSV 2019. 26th International Congress on Sound and Vibration, ICSV 2019, Montreal, Canada, 07/07/2019.

Stegmann, R & Schneuwly-Purdie, M 2019, A propos de l'héritage: Usages et enjeux pour les musulman.e.s de Suisse. <https://doc.rero.ch/record/327527/files/a5_csis_papers1_f_web.pdf>

Saksø, M, Andersen, E, Bentzen, J, Andersen, M, Johansen, J, Primdahl, H , Overgaard, J & Eriksen, JG 2019, 'A prospective, multicenter DAHANCA study of hyperfractionated, accelerated radiotherapy for head and neck squamous cell carcinoma', Acta Oncologica, vol. 58, no. 10, pp. 1495-1501. https://doi.org/10.1080/0284186X.2019.1658897

Rytter, S, Jensen, BG, Munk, S, Høvsgaard, SJ & Hansen, TB 2019, 'A prospective study of day of surgery discharge in 368 consecutive patients with unicompartmental knee replacement', Danish Medical Bulletin, vol. 66, no. 9, A5569.

Jakobsen, RL , Fuglsang-Frederiksen, A , Hellfritzsch, MB & Qerama, E 2019, 'A prospective study of high resolution ultrasound in brachial plexopathies: correlation with electrophysiological measurements', Clinical Neurophysiology, vol. 130, no. 7, pp. 1144-1150. https://doi.org/10.1016/j.clinph.2019.03.034

Pedersen, J , Mortensen, SO , Rölfing, JD & Thorninger, R 2019, 'A protocol for a single-center, single-blinded randomized-controlled trial investigating volar plating versus conservative treatment of unstable distal radius fractures in patients older than 65 years', BMC Musculoskeletal Disorders, vol. 20, no. 1, 309. https://doi.org/10.1186/s12891-019-2677-y

Hansen, MJ & Feilberg, A 2019, A protocol for chemical measurement of odour in relation to abatement technologies for animal production.

Deng, L, Silins, R, Castro-Mejía, JL, Kot, W, Jessen, L, Thorsen, J, Shah, S, Stokholm, J, Bisgaard, H, Moineau, S & Nielsen, DS 2019, 'A protocol for extraction of infective viromes suitable for metagenomics sequencing from low volume fecal samples', Viruses, vol. 11, no. 7, 667. https://doi.org/10.3390/v11070667

Góes, FL & Perez, CT 2019, Aproveitamento da água de chuva para irrigação do jardim em um condomínio residencial. in V SIMPÓSIO INTERNACIONAL DE INOVAÇÃO E TECNOLOGIA 2019 - SIINTEC. pp. 763-769 , V SIMPÓSIO INTERNACIONAL DE INOVAÇÃO E TECNOLOGIA 2019 - SIINTEC, Bahia, Brazil, 09/10/2019. https://doi.org/10.5151/siintec2019-95

Bjornsdottir, G, Ivarsdottir, EV, Bjarnadottir, K, Benonisdottir, S, Gylfadottir, SS, Arnadottir, GA, Benediktsson, R, Halldorsson, GH, Helgadottir, A, Jonasdottir, A, Jonasdottir, A, Jonsdottir, I, Kristinsdottir, AM, Magnusson, OT, Masson, G, Melsted, P, Rafnar, T, Sigurdsson, A, Sigurdsson, G, Skuladottir, A, Steinthorsdottir, V, Styrkarsdottir, U, Thorgeirsson, G, Thorleifsson, G, Vikingsson, A, Gudbjartsson, DF, Holm, H, Stefansson, H, Thorsteinsdottir, U, Norddahl, GL, Sulem, P, Thorgeirsson, TE & Stefansson, K 2019, 'A PRPH splice-donor variant associates with reduced sural nerve amplitude and risk of peripheral neuropathy', Nature Communications, vol. 10, no. 1, 1777. https://doi.org/10.1038/s41467-019-09719-4

Liu, L , Grombacher, D , Auken, E & Juul Larsen, J 2019, 'Apsu: A wireless multichannel receiver system for surface nuclear magnetic resonance groundwater investigations', Geoscientific Instrumentation, Methods and Data Systems, vol. 8, no. 1, pp. 1-11. https://doi.org/10.5194/gi-8-1-2019

Larsen, JJ , Liu, L , Grombacher, D , Osterman, G & Auken, E 2019, 'Apsu - A new surface nmr instrument for hydrogeophysics', Paper presented at 25th European Meeting of Environmental and Engineering Geophysics, Held at Near Surface Geoscience Conference and Exhibition 2019, NSG 2019, The Hague, Netherlands, 08/09/2019 - 12/09/2019.

Brand, BL, Spindler, H & Cannon, R 2019, A Psychological Assessment Perspective on Clinical and Conceptual Distinctions Between Dissociative Disorders and Psychotic Disorders. in Psychosis, Trauma and Dissociation: Evolving Perspectives on Severe Psychopathology, Second Edition. Wiley-Interscience, pp. 351-365. https://doi.org/10.1002/9781118585948.ch22

Sørensen, L, Wass, S, Osnes, B, Schanche, E, Adolfsdottir, S, Svendsen, JL, Visted, E, Eilertsen, T, Jensen, DA, Nordby, H, Fasmer, OB, Binder, PE, Koenig, J & Sonuga-Barke, E 2019, 'A psychophysiological investigation of the interplay between orienting and executive control during stimulus conflict: A heart rate variability study', Physiology & Behavior, vol. 211, 112657. https://doi.org/10.1016/j.physbeh.2019.112657

Skovsgaard, MB , Mortensen, MR , Palmfeldt, J & Gothelf, KV 2019, 'Aptamer-Directed Conjugation of DNA to Therapeutic Antibodies', Bioconjugate Chemistry, vol. 30, no. 8, pp. 2127-2135. https://doi.org/10.1021/acs.bioconjchem.9b00363

Park, S, Nielsen, A, Bailey, RT, Trolle, D & Bieger, K 2019, 'A QGIS-based graphical user interface for application and evaluation of SWAT-MODFLOW models', Environmental Modelling & Software, vol. 111, pp. 493-497. https://doi.org/10.1016/j.envsoft.2018.10.017

Buhl, TF, Christensen, CH & Hammershøj, M 2019, 'Aquafaba as an egg white substitute in food foams and emulsions: Protein composition and functional behavior', Food Hydrocolloids, vol. 96, pp. 354-364. https://doi.org/10.1016/j.foodhyd.2019.05.041

Dayyani, I, Terkildsen Maindal, H , Rowlands, G & Lou, S 2019, 'A qualitative study about the experiences of ethnic minority pregnant women with gestational diabetes', Scandinavian Journal of Caring Sciences, vol. 33, no. 3, pp. 621-631. https://doi.org/10.1111/scs.12655

Dayyani, I, Therkildsen Maindal, H, Rowlands, G & Lou, S 2019, 'A qualitative study about the experiences of ethnic minority pregnant women with gestational diabetes', Scandinavian Journal of Caring Sciences, vol. 33, pp. 621-631. https://doi.org/10.1111/scs.12655

Vested, A , Schlünssen, V , Burdorf, A , Andersen, JH, Christoffersen, J, Daugaard, S, Flachs, EM, Garde, AH, Hansen, ÅM, Markvart, J, Peters, S, Stokholm, Z , Vestergaard, JM , Vistisen, HT & Kolstad, HA 2019, 'A Quantitative General Population Job Exposure Matrix for Occupational Daytime Light Exposure', Annals of Work Exposures and Health, vol. 63, no. 6, pp. 666-678. https://doi.org/10.1093/annweh/wxz031

Joseph, S, Skibsted, J & Cizer, Ö 2019, 'A quantitative study of the C₃A hydration', Cement and Concrete Research, vol. 115, pp. 145-159. https://doi.org/10.1016/j.cemconres.2018.10.017

Login, FH , Jensen, HH , Pedersen, GA , Koffman, JS , Kwon, TH, Parsons, M & Nejsum, LN 2019, 'Aquaporins differentially regulate cell-cell adhesion in MDCK cells', The FASEB Journal, vol. 33, no. 6, pp. 6980-6994. https://doi.org/10.1096/fj.201802068RR

Hansen, AMB, Rawlings, J, Brill, J, Lam, M, Connors, K, Belanger, S & Sanderson, H 2019, 'Aquatic toxicity of cationic polymers', SETAC Europe, Helsinki, Finland, 26/05/2019 - 30/05/2019.

Pipitone, G, Zoppi, G, Frattini, A, Bocchini, S, Pirone, R & Bensaid, S 2019, 'Aqueous phase reforming of sugar-based biorefinery streams: from the simplicity of model compounds to the complexity of real feeds', Catalysis Today, vol. 345, pp. 267-279. https://doi.org/10.1016/j.cattod.2019.09.031

Pipitone, G, Zoppi, G, Bocchini, S, Rizzo, AM, Chiaramonti, D, Pirone, R & Bensaid, S 2019, 'Aqueous phase reforming of the residual waters derived from lignin-rich hydrothermal liquefaction: investigation of representative organic compounds and actual biorefinery streams', Catalysis Today, vol. 345, pp. 237-250. https://doi.org/10.1016/j.cattod.2019.09.040

Cicovic, A, Cicovic, S, Wong, B, Stottrup, NB, Ghattas, A & Glenie, T 2019, 'A Quicker Pace: Shockwave Lithotripsy Pacing With Electromechanical Capture', JACC: Cardiovascular Interventions, vol. 12, no. 17, pp. 1739-1740. https://doi.org/10.1016/j.jcin.2019.04.024

Casas, G, Lærke, HN , Knudsen, KEB & Stein, HH 2019, 'Arabinoxylan is the main polysaccharide in fiber from rice coproducts, and increased concentration of fiber decreases in vitro digestibility of dry matter', Animal Feed Science and Technology, vol. 247, pp. 255-261. https://doi.org/10.1016/j.anifeedsci.2018.11.017

Alvarez, MV, Bambace, MF & del Rosario Moreira, M 2019, Arándanos como alternativa no láctea en alimentos funcionales. Editorial Académica Española. EAE. <https://www.eae-publishing.com/catalog/details/store/gb/book/978-620-0-33162-5/ar%C3%A1ndanos-como-alternativa-no-l%C3%A1ctea-en-alimentos-funcionales>

Bernstorff, M , Deichgræber, P , Bruun, NH , Dalsgaard, E-M , Fenger-Grøn, M & Lauritzen, T 2019, 'A Randomised Trial Examining Cardiovascular Morbidity and All-Cause Mortality 24 years Following General Health Checks: the Ebeltoft Health Promotion Project (EHPP)', BMJ Open, vol. 9, no. 10, e030400. https://doi.org/10.1136/bmjopen-2019-030400

Stjerneklar, S , Hougaard, E, McLellan, LF & Thastum, M 2019, 'A randomized controlled trial examining the efficacy of an internet-based cognitive behavioral therapy program for adolescents with anxiety disorders', PLoS One, vol. 14, no. 9, e0222485. https://doi.org/10.1371/journal.pone.0222485

Jespersen, KV , Otto, M , Kringelbach, ML, Van Someren, E & Vuust, P 2019, 'A randomized controlled trial of bedtime music for insomnia disorder', Journal of Sleep Research, vol. 28, no. 4, e12817. https://doi.org/10.1111/jsr.12817

Bendszus, M, Bonekamp, S, Berge, E, Boutitie, F, Brouwer, P, Gizewski, E, Krajina, A, Pierot, L, Randall, G, Simonsen, CZ, Zeleňák, K, Fiehler, J & Thomalla, G 2019, 'A randomized controlled trial to test efficacy and safety of thrombectomy in stroke with extended lesion and extended time window', International Journal of Stroke, vol. 14, no. 1, pp. 87-93. https://doi.org/10.1177/1747493018798558

Boggett, S, Ou-Young, J, Heiberg, J, De Steiger, R, Richardson, M, Williams, Z & Royse, C 2019, 'A randomized trial of desflurane or sevoflurane on postoperative quality of recovery after knee arthroscopy', PLoS One, vol. 14, no. 8, e0220733. https://doi.org/10.1371/journal.pone.0220733

Lehmann, S, Ringbaek, T, Lokke, A, Grote, L, Hedner, J & Lindberg, E 2019, 'A randomized trial to determine the impact of indacaterol/glycopyrronium on nighttime oxygenation and symptoms in patients with moderate-to-severe COPD: the DuoSleep study', International Journal of Chronic Obstructive Pulmonary Disease, vol. 14, pp. 199-210. https://doi.org/10.2147/COPD.S184127

Raynolds, MK, Walker, DA, Balser, A, Bay, C, Campbell, M, Cherosov, MM, Daniëls, FJA, Eidesen, PB, Ermokhina, KA, Frost, GV, Jedrzejek, B, Jorgenson, MT, Kennedy, BE, Kholod, SS, Lavrinenko, IA, Lavrinenko, OV, Magnússon, B, Matveyeva, NV, Metúsalemsson, S, Nilsen, L, Olthof, I, Pospelov, IN, Pospelova, EB, Pouliot, D, Razzhivin, V, Schaepman-Strub, G, Šibík, J, Telyatnikov, MY & Troeva, E 2019, 'A raster version of the Circumpolar Arctic Vegetation Map (CAVM)', Remote Sensing of Environment, vol. 232, 111297. https://doi.org/10.1016/j.rse.2019.111297

Hansen, BB 2019, 'Arbejdet bærer skylden i sig selv', Dagbladet Information.

Bønløkke, JH, Duchaine, C, Schlünssen, V , Sigsgaard, T, Veillette, M & Basinas, I 2019, 'Archaea and Bacteria Exposure in Danish Livestock Farmers', Annals of Work Exposures and Health, vol. 63, no. 9, pp. 965-974. https://doi.org/10.1093/annweh/wxz058

Shepherd, N 2019, 'Archaeology in the Shadow of Apartheid: Race, Science and Prehistory', South African Archaeological Society Goodwin Series, vol. 12, pp. 13-21.

Kost, C & Hussain, ST 2019, 'Archaeo-Ornithology: Towards an Archaeology of Human-Bird Interfaces', Environmental Archaeology, vol. 24, no. 4, pp. 337-358. https://doi.org/10.1080/14614103.2019.1590984

Kristensen, HK 2019, Archäologie, Bau und Kunstgeschichte. in O Auge & K Hillebrand (eds), Klosterbuch Schleswig-Holstein und Hamburg: Klöster, Stifte und Konvente von den Anfängen bis zur Reformation. vol. 1, Schnell & Steiner, Regensburg, pp. 669-688.

Kumar, A & Mehta, PL 2019, Architectural Framework for Good Data: A Realm for General Data Protection Regulation. in 2018 21st International Symposium on Wireless Personal Multimedia Communications, WPMC 2018., 8712907, IEEE, International Symposium on Wireless Personal Multimedia Communications, WPMC, vol. 2018-November, pp. 375-380, 21st International Symposium on Wireless Personal Multimedia Communications, WPMC 2018, Chiang Rai, Thailand, 25/11/2018. https://doi.org/10.1109/WPMC.2018.8712907

Agostinho, D 2019, 'Archival Encounters: Rethinking access and care in digital colonial archives', Archival Science, vol. 19, no. 2, pp. 141-165. https://doi.org/10.1007/s10502-019-09312-0

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