Aarhus University Seal

Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Larsen, CR (ed.) 2003, Kulturpolitisk forskning og debat 3: Kunstråd. Institut for Dramaturgi, Aarhus Universitet.
Rasmussen, HN, Andersen, L & Sørensen, S 2003, 'Kulturstart i nordmannsgran 1: Baggrund, forsøg og rodresultater', Videnblad, no. 4.9-5.
Rasmussen, HN, Andersen, L & Sørensen, S 2003, 'Kulturstart i nordmannsgran 2: Forøgelse af stammetilvæksten', Videnblad, no. 4.9-6.
Rasmussen, HN, Andersen, L & Sørensen, S 2003, Kulturstart i nordmannsgran - knopmanipulation, farer og fordele. in Skov & Landskabskonferencen, Skov & Landskab, Hørsholm, 2003. pp. 89-92, Skov & Landskabskonferencen, Hørsholm, Denmark, 18/12/2010.
Eskildsen, JK 2003, 'Kundetilfredshedsmodeller er dynamiske', CFL Nyhedsbrev, no. september.
Korsgaard, O 2003, Kundskabsproduktion som globaliseringens drivkraft. in Folkeskolen: Ekstern tilpasning og intern organisering. Billesø, Værløse.
Thomsen, IK 2003, 'Kun lille N-effekt af jordløsning i vårbyg', Landsbladet - Landbrugsmagasinet, pp. 32-32.
Nissen, UB 2003, 'Kunsten af fortolke', Kristeligt Dagblad, pp. 6.
Hansen, HP 2003, 'Kunsten at gøre en forskel', Jæger, vol. 12, no. 9, pp. 102-105.
Nielsen, HK & nullrum46, K (ed.) 2003, Kunsten og den demokratiske offentlighed. in Takkekortet. rum46, Århus.
Hansen, E 2003, Kunstens abstraherende karakter: Om at kvalificere og genindføre diskussionen af kunst. in SL Jørgensen, T Haunstrup & E Dalsgaard (eds), Fra verden til navlen: Kunsten i funktion. Forlaget Philosophia, Aarhus, pp. 87-112.
Sørensen, LN 2003, Kunst(fotografi). in From China. Århus Kunstbygning og Galleri Image, Århus, pp. 6-11.
Frederiksen, HJ, Frederiksen, HJ (ed.) & Sommer, A-L (ed.) 2003, Kunsthistoriske bemærkninger til Flannery O Connors novelle "Parkers ryg" in Kunsthistoriske kontakter: Tværfaglige dialoger. Aarhus Universitetsforlag, Århus, pp. 187-199.
Humaidan, PS, Agerholm, I, Ingerslev, HJ, Pedersen, C, Gerstoft, J & Andersen, AN 2003, 'Kunstig befrugtning af hiv-positive--en risikoreducerende behandling', Ugeskrift for Læger, vol. 165, no. 48, pp. 4632-5.
Nielsen, HK & Jørgensen, SL (ed.) 2003, Kunst og samfund. in Fra verden til navlen. Forlaget Philosophia, Århus.
Langsted, J 2003, Kunstrådet: Kulturpolitisk forskning og debat 2. Institut for Dramaturgi, Århus.
Kyndrup, M 2003 'Kunstværk og udsigelse' ACTS 18 edn, Æstetik og Kultur, Aarhus Universitet, Århus.
Bolander, J 2003, Kursgevinstloven. in Bo von Eyben, Jan Pedersen, Thomas Rørdam (red.) (ed.), Karnovs lovsamling med kommentarer. Karnov Group, København, pp. s. 1456-1481.
Moser, T, Didriksen, AN & Christiansen, K 2003, Kurz- und Langzeiteffekte psychomotorischen Trainings auf die Grobmotorik von Kindern mit schwachen motorischen Voraussetzungen. in Towards a Society for All through Adapted Physical Activity. Vienna.
Børsting, CF 2003, Kvægbrugets Forsøgscenter arbejder for det praktiske kvægbrug. in Årsrapport 2002. Dansk Kvæg, Årsrapport 2002, pp. 50-51.
Sørensen, P 2003, 'Kvæggylle skal nedfældes for at opnå høj udnyttelse', Ny KvægForskning, vol. 1, no. 6, pp. 2-2.
Petersen, JK & Hallonsten, AL 2003, 'Kvælstofforilte/sedation i tandplejen', Tandlægebladet, vol. 107, pp. 316-323.
Vinther, FP 2003, 'Kvælstof forsvinder', Økologisk Jordbrug, vol. 297, pp. 8-8.
Vinther, FP 2003, 'Kvælstof fra luften', Økologisk Jordbrug, vol. 288, pp. 6-6.
Danielsen, VO 2003, 'Kvælstof i gyllen kan reduceres', SVIN, Svineproducentens Fagmagasin, vol. 10, pp. 34-34.
Søegaard, K 2003, Kvælstof til kløvergræs under forskellige benyttelsesformer. in Grovfoderseminar 2003. Landbrugets Rådgivningscenter. Kvælstof til kløvergræs under forskellige benyttelsesformer, 18/12/2010. <http://www.lr.dk/planteavl/informationsserier/grovfoderseminar2003.htm>
Grevsen, K 2003, 'Kvalitet af blomkål ved dækning mod skadedyr. I: Dækkematerialer', Frugt og Gront, vol. 2, no. 2, pp. 70-73.
Jensen, SK 2003, Kvalitet af fedt og fedttyper til grise. in Fodringsseminar 30. April 2003, Herning Kongrescenter. Landsudvalget for Svin.. Kvalitet af fedt og fedttyper til grise, 18/12/2010.
Juhl, HJ, Kristensen, K, Eskildsen, JK & Østergaard, P 2003, Kvalitet i den danske dagligvaresektor - udvikling, kundetilfredshed og kundeloyalitet. in Kai Kristensen og Henrik D. Sørensen (red.) (ed.), Total Quality Management - Børsen Ledelseshåndbøger. Børsen Forum A/S, København, pp. Afsn. 9.2.
Jensen, M 2003, 'Kvalitetssortering af træfrø: Ét frø - en førstekvalitetsplante', Gartner Tidende, vol. 119, no. 12, pp. 4-5.
Larsen, L & Petersen, II 2003, 'Kvalitetsudvikling af gerontopsykiatrisk omsorg', Gerontologi og Samfund, vol. 19, no. 2, pp. 34-37.
Baadsgaard, NP, Nielsen, NC & Enevoldsen, C 2003, 'Kvantificering af den kliniske sundhedstilstand i praksis', Dansk Veterinærtidsskrift, vol. 1, pp. 12-18.
Nielsen, K, Thorsen, M, Markager, SS, Jensen, JP, Søndergaard, M, Refsgaard, JC, Styczen, M, Dahl-Madsen, KI, Duus Børgesen, CD, Wiggers, L, Pedersen, SE & Madsen, HB 2003, Kvantificering af næringsstoffers transport fra kilde til recipient samt effekt i vandmiljøet. Faglig rapport fra DMU, vol. 455, Danmarks Miljøundersøgelser, Aarhus Universitet. <http://www.dmu.dk/1_viden/2_Publikationer/3_fagrapporter/rapporter/FR455.pdf>
Rasmussen, IA, Askegaard, M, Olesen, JE & Thomsen, HC 2003, 'Kvik volder kvaler', Økologisk Jordbrug, vol. 23, no. 288, pp. 13-13.
Kratschmer, A 2003, 'L'arte del ben vivere e del ben morire i den italienske Renaissance: Semantiske felter', Renæssanceforum: Tidsskrift for Renæssanceforskning, no. 189, pp. 70-81.
Veien, N 2003, 'Label dermatitis.', Dermatitis, vol. 14, no. 2, pp. 104.
Roepstorff, A & Hastrup, K (ed.) 2003, Laboratoriet - Stedet for erkendelse. in Ind i verden. En grundbog i antropologisk metode. Hans Reitzels Forlag, pp. 365-380.
Sørensen, CAG & Nielsen, V 2003, Labour and machinery systems in organic farming. in Proc. XXX Ciosta-CIGR V Congress. pp. 525-533, Management and technology applications to empower agriculture and agro-food systems, Torino, Italy, 18/12/2010. <https://orgprints.org/id/eprint/2435/>
Westergård-Nielsen, NC, Agerbo, E, Eriksson, TV & Mortensen, PB 2003, 'Labour Market Behaviour When Minds Are Not So Beautiful. An Analysis of Schizophrenia and Affective Diseases and the Labour Market', Paper presented at American Economic Association, Washington DC, USA, 03/01/2003 - 05/01/2003.