Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Krejsler, JB 2019, How a European 'Fear of Falling Behind' Discourse Co-produces Global Standards: exploring the Inbound and Outbound Performativity of the Transnational Turn in European Education Policy. in C Ydesen (ed.), The OECD's Historical Rise in Education: the Formation of a Global Governing Complex., 10.1007/978-3-030-33799-5_12, Palgrave Macmillan, Cham, Global Histories of Education, pp. 245-267.

Andreassen, P & Malling, BVG 2019, 'How are formative assessment methods used in the clinical setting? A qualitative study', International Journal of Medical Education, vol. 10, pp. 208-215. https://doi.org/10.5116/ijme.5db3.62e3

Anderson, RH, Niederer, PF, Sanchez-Quintana, D, Stephenson, RS & Agger, P 2019, 'How are the cardiomyocytes aggregated together within the walls of the left ventricular cone?', Journal of Anatomy, vol. 235, no. 4, pp. 697-705. https://doi.org/10.1111/joa.13027

Wamberg, J 2019, How art became posthuman: towards an extended historiography. in M Chybowska-Butler, M Lewoc & M Renberg (eds), Living in the present future. Bornholms Kunstmuseum and Muzeum Narodowe a Szczecinie, Gudhjem, pp. 44-54.

Jahn, S, Tsalis, G & Lähteenmäki, L 2019, 'How attitude towards food fortification can lead to purchase intention', Appetite, vol. 133, no. February, pp. 370-377. https://doi.org/10.1016/j.appet.2018.11.022

Andersen, C, Rosenstroem, CA & Ruchayskiy, O 2019, 'How bright was the Big Bang?', American Journal of Physics, vol. 87, no. 5, pp. 395-400. https://doi.org/10.1119/1.5092705

Persson, P, Kallivokas, L, Andersen, LV & T. Peplow, A 2019, How building adjacency affects occupant-perceivable vibrations due to urban sources: A parametric study. in M Papadrakakis & M Fragiadakis (eds), Proceedings of the 7th International Conference on Computational Methods in Structural Dynamics and Earthquake Engineering. vol. 3, National Technical University of Athens, pp. 5610-5621, 7th International Conference on Computational Methods in Structural Dynamics and Earthquake Engineering, Crete, Greece, 24/06/2019. https://doi.org/10.7712/120119.7331.18833

Wang, QJ 2019, 'How can VR be adapted for immersive dining situations? Two case studies on flavour and aroma perception', Food and Hospitality Multisensory Perspectives Symposium, Lyon, France, 15/11/2019 - 15/11/2019.

Aschemann-Witzel, J & Peschel, AO 2019, 'How circular will you eat? The sustainability challenge in food and consumer reaction to either waste-to-value or yet underused novel ingredients in food.', Food Quality and Preference, vol. 77, pp. 15-20. https://doi.org/10.1016/j.foodqual.2019.04.012

Heilmann-Clausen, J, Bruun, HH, Ejrnæs, R, Frøslev, TG, Læssøe, T & Petersen, JH 2019, 'How citizen science boosted primary knowledge on fungal biodiversity in Denmark', Biological Conservation, vol. 237, no. September, pp. 366-372. https://doi.org/10.1016/j.biocon.2019.07.008

Jørgensen, BM & Bager-Elsborg, A 2019, 'How context influences on students' engagement with feedback', Journal of Higher Education.

Calbi, M, Siri, F, Heimann, K, Barratt, D, Gallese, V, Kolesnikov , A & Umiltà, MA 2019, 'How context influences the interpretation of facial expressions: a source localization high-density EEG study on the “Kuleshov effect”', Scientific Reports, vol. 9, 2107. https://doi.org/10.1038/s41598-018-37786-y

Prilop, CN, Weber, KE & Kleinknecht, M 2019, 'How Digital Coaching Environments Contribute to Preservice Teachers' Self-Efficacy and Beliefs About Teaching and Learning', Annual Meeting of the American Educational Research Association (AERA), Toronto, Canada, 11/03/2019.

Prilop, CN, Weber, KE & Kleinknecht, M 2019, 'How digital reflection and feedback environments contribute to pre-service teachers’ beliefs during a teaching practicum', Studies in Educational Evaluation, vol. 62, pp. 158-170.

Kröner, SM & Beedholm, K 2019, 'How discourses of social vulnerability can influence nurse-patient interactions: A Foucauldian analysis', Nursing Inquiry, vol. 26, no. 4, e12309. https://doi.org/10.1111/nin.12309

Korsgaard, B , Nielsen, LP & Boesen, T 2019, 'How Do Bacteria Conduct Electrons? Towards structural and functional knowledge of the Desulfovibrio alkaliphilus conductive pili', Nordic Workshop on Scattering from Soft Matter, Aarhus, Denmark, 09/01/2019 - 10/01/2019.

Sanderson, H, Morales Irato, D, Cerezo, NP, Duel, H, Faria, P & Torres, EF 2019, 'How do climate risks affect corporations and how could they address these risks?', SN Applied Sciences, vol. 1, no. 12, 1720. https://doi.org/10.1007/s42452-019-1725-4

Whiteley, P & Kölln, A-K 2019, 'How Do Different Sources of Partisanship Influence Government Accountability in Europe?', International Political Science Review, vol. 40, no. 4, pp. 502-517. https://doi.org/10.1177/0192512118780445

Nielsen, AA 2019, 'How does informal and formal social control affect law violations?', Aarhus. <http://politica.dk/fileadmin/politica/Dokumenter/ph.d.-afhandlinger/alva_albaek_nielsen.pdf>

Poulsen, PH , Biering, K , Winding, TN , Nohr, EA , Petersen, LV, Ulijaszek, SJ & Andersen, JH 2019, 'How does psychosocial stress affect the relationship between socioeconomic disadvantage and overweight and obesity? Examining Hemmingsson's model with data from a Danish longitudinal study', B M C Public Health, vol. 19, no. 1, 1475. https://doi.org/10.1186/s12889-019-7699-8

Laursen, R 2019, '"How does school management support teachers working wellbeing when implementing a learning management system"', Paper presented at International Congress for School Effectiveness and Improvement , Stavanger, Norway, 07/01/2019 - 11/01/2019.

Klitgaard, MB & Paster, T 2019 'How Do Governments Respond to Business Demands for Tax Cuts: An analysis of Corporate and Inheritance Tax Reforms in Austria and Sweden' Minda de Gunzburg Center for European Studies - Harvard University, Cambridge, MA.

Gundlach, E & Svendsen, GT 2019, 'How do high and low levels of social trust affect the long-run performance of poor economies?', Journal of International Development, vol. 31, no. 1, pp. 3-21. https://doi.org/10.1002/jid.3388

Sørensen, J & Nielbo, KL 2019, How do humans process ritualized actions? in J Slone & W McCorkle (eds), The Cognitive Science of Religion: A Methodological Introduction to Key Empirical Studies. Bloomsbury Academic.

Madsen, RB & Glasius, M 2019, 'How Do Hydrothermal Liquefaction Conditions and Feedstock Type Influence Product Distribution and Elemental Composition?', Industrial and Engineering Chemistry Research, vol. 58, no. 37, pp. 17583-17600. https://doi.org/10.1021/acs.iecr.9b02337

Haas, R, Imami, D, Miftari, I, Ymerie, P & Grunert, K 2019, 'How do kosovar and albanian consumers perceive food quality and safety in the dairy sector?', Studies in Agricultural Economics, vol. 121, no. 3, pp. 119-126. https://doi.org/10.7896/j.1920

Lundorff, M , Thomsen, DK, Damkier, A & O'Connor, M 2019, 'How do loss- and restoration-oriented coping change across time? A prospective study on adjustment following spousal bereavement', Anxiety, Stress and Coping, vol. 32, no. 3, pp. 270-285. https://doi.org/10.1080/10615806.2019.1587751

Wendel-Hansen, J 2019, How Donald Trump’s proposal to buy Greenland really went down in Denmark. <https://theconversation.com/how-donald-trumps-proposal-to-buy-greenland-really-went-down-in-denmark-122160>

Karg, ST, Kim, M, Mitkidis, P & Young, L 2019, 'How do people judge the moral character of their leader and how do they update their judgements given new information?', SIPS 2019, 07/07/2019 - 09/07/2019.

Møller, JE & Brøgger, MN 2019, 'How do residents perceive and narrate stories about communication challenges in patient encounters? A narrative study', BMJ Open, vol. 9, no. 6, e029022. https://doi.org/10.1136/bmjopen-2019-029022

Møller, FS 2019, 'How do sources of traditional legitimacy constrain popular uprisings? The case of the Kingdom of Swaziland', Small Wars and Insurgencies, vol. 30, no. 2, pp. 392-420. https://doi.org/10.1080/09592318.2019.1602978

Jeppesen, MM, Mogensen, O, Hansen, DG, Bergholdt, SH & Jensen, PT 2019, 'How Do We Follow Up Patients With Endometrial Cancer?', Current Oncology Reports, vol. 21, 57. https://doi.org/10.1007/s11912-019-0805-3

Ambrosio, T & Tolstrup, J 2019, 'How do we tell Authoritarian Diffusion from Illusion? Exploring Methodological Issues of Qualitative Research on Authoritarian Diffusion', Quality and Quantity, vol. 53, no. 6, pp. 2741-2763. https://doi.org/10.1007/s11135-019-00892-8

Orso, R, Creutzberg, KC, Wearick-Silva, LE, Wendt Viola, T, Tractenberg, SG, Benetti, F & Grassi-Oliveira, R 2019, 'How Early Life Stress Impact Maternal Care: A Systematic Review of Rodent Studies', Frontiers in Behavioral Neuroscience, vol. 13, 197. https://doi.org/10.3389/fnbeh.2019.00197

Brøgger, K 2019, 'How Education Standards Gain Hegemonic Power and become International: The case of higher education and the Bologna Process', European Educational Research Journal, vol. 18, no. 2, pp. 158-180. https://doi.org/10.1177/1474904118790303

Harris, M, Thulesius, H, Neves, AL, Harker, S, Koskela, T, Petek, D, Hoffman, R, Brekke, M, Buczkowski, K, Buono, N, Costiug, E, Dinant, GJ, Foreva, G, Jakob, E, Marzo, M, Murchie, P, Sawicka-Powierza, J, Schneider, A, Smyrnakis, E, Streit, S, Taylor, G, Vedsted, P, Weltermann, B & Esteva, M 2019, 'How European primary care practitioners think the timeliness of cancer diagnosis can be improved: a thematic analysis', BMJ Open, vol. 9, no. 9, e030169, pp. e030169. https://doi.org/10.1136/bmjopen-2019-030169

Krieger, J 2019, How Fears Changed the Games: Of Terror, Security and Costs. in J Krieger & S Wassong (eds), Dark Sides of Sport. Common Ground Publishing, Champaign, IL, The International Journal of Sport and Society, pp. 57-72.

Svensson, PC & Pasgaard, M 2019, 'How geoengineering scientists perceive their role in climate security politics–from concern and unease to strategic positioning', Geografisk Tidsskrift - Danish Journal of Geography, vol. 119, no. 1, pp. 84-93. https://doi.org/10.1080/00167223.2019.1573352

Bisgaard, M 2019, 'How Getting the Facts Right Can Fuel Partisan-Motivated Reasoning', American Journal of Political Science, vol. 63, no. 4, pp. 824-839. https://doi.org/10.1111/ajps.12432

Hansen, FG 2019, 'How Impressions of Public Employees’ Warmth and Competence Influence Trust in Government', EGPA 2019 , Belfast, United Kingdom, 09/09/2019 - 13/09/2019.

Aggerholm, HK, Asmuß, B, Boivin, G, Buttny, R & Krippendorff, K 2019, How institutional authority and routine exertions of power can be mobilized, negotiated and challenged. in N Bencherki, F Matte & F Cooreen (eds), Authority and Power in Social Interaction: Methods and Analysis. Routledge, New York, pp. 56-76.

Xue, M, Wakamoto, T, Kejlberg, C , Yoshimura, Y , Nielsen, TA , Risør, MW , Sanggaard, KW, Kitahara, R & Mulder, FAA 2019, 'How internal cavities destabilize a protein', Proceedings of the National Academy of Sciences (PNAS), vol. 116, no. 42, pp. 21031-21036. https://doi.org/10.1073/pnas.1911181116

Salgado, S & Kingo, OS 2019, 'How is physiological arousal related to self-reported measures of emotional intensity and valence of events and their autobiographical memories?', Consciousness and Cognition, vol. 75, 102811. https://doi.org/10.1016/j.concog.2019.102811

Chen, X , Ottosen, LDM & Kofoed, MVW 2019, 'How Low Can You Go: Methane Production of Methanobacterium congolense at Low CO2 Concentrations', Frontiers in Bioengineering and Biotechnology, vol. 7, 00034. https://doi.org/10.3389/fbioe.2019.00034

Pedersen, RØ 2019, 'How mammals shape the world'.

Vase, L , Baad-Hansen, L & Pigg, M 2019, 'How May Placebo Mechanisms Influence Orofacial Neuropathic Pain?', Journal of Dental Research, vol. 98, no. 8, pp. 861-869. https://doi.org/10.1177/0022034519848719

Callesen, J , Dalgas, U , Brincks, J & Cattaneo, D 2019, 'How much does balance and muscle strength impact walking in persons with multiple sclerosis? - A cross-sectional study', Multiple Sclerosis and Related Disorders, vol. 29, pp. 137-144. https://doi.org/10.1016/j.msard.2019.01.034

Enevoldsen, P, Permien, FH, Bakhtaoui, I, Krauland, AKV, Jacobson, MZ, Xydis, G , Sovacool, BK, Valentine, SV, Luecht, D & Oxley, G 2019, 'How much wind power potential does Europe have? Examining European wind power potential with an enhanced socio-technical atlas', Energy Policy, vol. 132, pp. 1092-1100. https://doi.org/10.1016/j.enpol.2019.06.064

Hansen, NB , Dindler, C , Halskov, K , Iversen, OS , Bossen, C , Basballe, DA & Schouten, B 2019, How participatory designworks: Mechanisms and effects. in Proceedings of the 31st Australian Conference on Human-Computer-Interaction, OzCHI 2019. Association for Computing Machinery, pp. 30-41, 31st Australian Conference on Human-Computer-Interaction, OzCHI 2019, Perth/Fremantle, Australia, 02/12/2019. https://doi.org/10.1145/3369457.3369460

Hansen, NB , Dindler, C , Halskov, K , Iversen, OS , Bossen, C , Basballe, DA & Schouten, B 2019, How Participatory Design Works: Mechanisms and Effects. in OzCHI: Australian Conference on Human-Computer Interaction Proccedings. AUSTRALIAN CONFERENCE ON HUMAN-COMPUTER-INTERACTION , Fremantle, Western Australia, Australia, 03/12/2019.

Displaying results 98751 to 98800 out of 389757

1976

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen