Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Britz, D & Britz, TJ 2003, 'Mathematical proof of the consistency of Feldberg's simple BDF start in electrochemical digital simulations', Journal of Electroanalytical Chemistry, vol. 546, pp. 123-125.

Misfeldt, M 2003, Mathematician's Writing. in N Pateman, B Dougerty & J Zilliox (eds), Proceedings of the 27th Conference on the Psychology of Mathematics Education. vol. 3, University of Hawaii, pp. 301-308, The 27th Conference on the Psychology of Mathematics Education, Honolulu, United States, 13/07/2003.

Pedersen, JL (Translator) 2003, 'Maurice Blanchot: "En urscene?"', Passepartout, no. 22, pp. 8-8.

Funch-Jensen, P , Christensen, LA & Kruse, A 2003, 'Mavesår', Medicus 2003, vol. 33, pp. 3-6.

Engberg, RM, Knarreborg, A, Bjerrum, L & Jensen, BB 2003, 'Mave-tarm kanalens mikrobiologi og dens påvirkning gennem fodring', Gemensam Nordisk Fjäderfätidskrift, november, vol. 2003, pp. 30-35.

Thomsen, P, Bleses, D, Lindegaard, M & Madsen, TO 2003, "Må vi bede om lidt fælles opmærksomhed! Nogle tanker om betydningen af fælles opmærksomhed og imitativ læring for barnets tidlige sprogtilegnelse". in HG Jacobsen, red., m.fl. (ed.), Take Danish for instance: Linguistic studies in honour of Hans Basbøll presented on the occasion of his 60th birthday 12 July 2003. Syddansk Universitetsforlag, Odense, pp. 283-294.

Perregaard, B 2003, Må vi skrive på vores historie? Børns organisering og udnyttelse af skriftsprog. 1. udgave edn, Akademisk Forlag, København.

Agarkova, I, Ehler, E, Lange, S, Schoenauer, R & Perriard, J-C 2003, 'M-band: a safeguard for sarcomere stability?', Journal of Muscle Research and Cell Motility, vol. 24, no. 2-3, pp. 191-203. https://doi.org/10.1023/a:1026094924677

Gaiduk, PI, Nylandsted Larsen, A & Hansen, JL 2003, 'MBE grown Ge dots on Si/SiGe ion implanted buffers', Physica B, no. 17, pp. 510.

Neto, PR, Olsen, AK , Jensen, SB , Munk, OL, Watanabe, H, Gjedde, A & Cumming, P 2003, ' MDMA-evoked changes in 11C-raclopride and 11C-NMSP binding potentials.', European Neuropsychopharmacology, pp. S453.

Neto, PR, Olsen, AK , Jensen, SB , Munk, OL, Watanabe, H, Gjedde, A & Cumming, P 2003, 'MDMA-evoked competition between [11C]raclopride and endogenous dopamine', Brain03 & BrainPET03, 17/12/2010.

Spokes, L, Brandt, J , Carstensen, J, Christiansen, T, Conley, D, Frohn, LM, Geernaert, G, Gustafsson, B, Bay Hasager, C, Henriksen, P , Hertel, O , Jensen, B, Jickells, T, Johnson, M, Kelly, S, Liljebladh, B, Lund, S, Lundsgaard, C, Markager, S, Martinsen, W, Møller, BL, Pedersen, B, Pryor, S, Sauerberg, K, Sempreviva, AM, Sørensen, LL, Svensson, G, Tjernström, M, Weston, K & Zagar, M 2003, MEAD - Marine Effects of Atmospheric Deposition: MEAD final report.

Hasager, CB, Carstensen, J , Frohn, LM, Gustafson, B, Brandt, J, Conley, D, Geernaert, G, Henriksen, P, Ambelas Skjøth, C & Johnsson, M 2003, MEAD Retrospective Analysis Report. Risø National Laboratory, Roskilde.

Cold, G 2003, 'Mean arterial pressure and intracranial pressure (correspondance)', Anesthesiology, vol. 99.

Müller, HH 2003, 'Meaning Construction within Nominal Syntagmatic Compounds (NSCs)'.

Kraaij, R, Mayer, P, Busser, FJM, Bolscher, MVH, Seinen, W, Tolls, J & Belfroid, AC 2003, 'Measured Pore-Water Concentrations Make Equilibrium Partitioning Work - A Data Analysis', Environmental Science & Technology, vol. 37, no. 2, pp. 268-274.

Sørensen, A & Mølmer, K 2003, 'Measurement induced entanglement and quantum computation with atoms in optical cavities', Physical Review Letters, vol. 91, pp. 097905.

Nielsen, T 2003, Measurement of Skin Perfusion and Blood Oxygenation Response using Surface Coil based 1H NMR Spectroscopy and SSFP Imaging - Technical Aspects. Aarhus Universitet, Århus.

Nielsen, T 2003, Measurement of Skin Perfusion and Blood Oxygenation Response using Surface Coil based 1H NMR Spectroscopy and SSFP Imaging - Technical Aspects. Aarhus Universitet.

Jensen, J, Kinch, K, Merrison, J & Nørnberg, P 2003 'Measurement of the electrical properties of suspended Martian dust' Nice, France, pp. 0-0.

Hellweg, D, Cacciapuoti, L, Kottke, M, Schulte, T, Sengstock, K, Ertmer, W & Arlt, JJ 2003, 'Measurement of the Spatial Correlation Function of Phase Fluctuating Bose-Einstein Condensates', Phys. Rev. Lett. 97, vol. 91, no. 1, pp. 010406. https://doi.org/10.1103/PhysRevLett.91.010406

Skov, H, Nielsdóttir, MC, Goodsite, ME, Christensen, J, Ambelas Skjøth, C, Geernaert, G, Hertel, O & Olsen, J 2003, 'Measurements and modelling of gaseous elemental mercury (GEM) on the Faroe Islands: a case study of the difficulties of measuring GEM', Asian Chemistry Letters, vol. 7, no. 2 & 3, pp. 151-158.

Nordsmark, M, Loncaster, J, Aquino-Parsons, C, Chou, S-C, Ladekarl, M, Havsteen, H, Lindegaard, JC, Davidson, SE, Varia, M, West, C, Hunter, R, Overgaard, J & Raleigh, JA 2003, 'Measurements of hypoxia using pimonidazole and polarograhpic oxygen-sensitive electrodes in human cervix carcinomas', Radiotherapy & Oncology, vol. 67, no. 1, pp. 35-44. https://doi.org/10.1016/S0167-8140(03)00010-0

Wåhlin, P, Mønster, JG, Glasius, M, Ketzel, M, Berkowicz, R & Palmgren, F 2003, 'Measurements of wood burning emissions in denmark - first results', NOSA 2003 Aerosol Symposium, Copenhagen, Denmark, 13/11/2003 - 14/11/2003.

Østergaard, P , Juhl, HJ , Kristensen, K & Eskildsen, JK 2003, Measuring Customer Satisfaction and Loyalty in the Danish Automobile Industry. in Proceedings of the 7th International Conference: Corporate Reputation, Image, and Identity. pp. 1-28, 7th International Conference: Corporate Reputation, Image, and Identity / Reputation Institute, Manchester, United Kingdom, 22/05/2003.

Holm, C & Steenholdt, N 2003, Measuring Learning Outcomes in Auditing Education. in Niels Kærgaard (red.) (ed.), Symposium i anvendt statistik. Den Kgl. Veterinær- og Landbohøjskole, København, pp. s. 81-92.

Poolton, NRJ, Bøtter-Jensen, L, Andersen, CE, Jain, M, Murray, AS, Malins, AER & Quinn, FM 2003, 'Measuring modulated luminescence using non-modulated stimulation: Ramping the sample period', Radiation Measurements, vol. 37, no. 6, pp. 639-645. https://doi.org/10.1016/S1350-4487(03)00244-0

Rehling, M & Peters, AM (ed.) 2003, Measuring Renal Function. in Nuclear Medicine in Radiological Diagnosis. Martin Dunitz, Taylor, London, pp. 163-179.

Bjørnskov, C & Svendsen, GT 2003 'Measuring social capital: Is there a single underlying explanation?' Aarhus School of Business.

Lippert-Rasmussen, K 2003, 'Measuring the disvalue of inequality over time', Theoria, vol. 69, no. 1-2, pp. 32-45.

Lippert-Rasmussen, K 2003, 'Measuring the Disvalue of Inquality Through Time', Theoria, no. 69, pp. 32-45.

Rasmussen, MD, Reinemann, DJ & Mein, GA 2003, 'Measuring Vacuum in Milking Machines', Bulletin of the International Dairy Federation, vol. 381, pp. 19-32.

Milana, M 2003, 'Meccanismi di co-finanziamento per favorire l?apprendimento permanente in Europa', Professionalita, no. 75, pp. 19-24. <http://www.lascuola.it/webapp/servlet/NavigationServlet?pAction=showDetailRivista&pRivista=1100595295156>

Nygaard, JV 2003, 'Mechanical characterisation of microcellular thermoplastic foams manufactured using gas diffusion technique', Aalborg.

Christensen, TD , Andersen, NT , Attermann, J , Hjortdal, VE , Maegaard, M & Hasenkam, JM 2003, 'Mechanical heart valve patients can manage oral anticoagulant therapy themselves', European Journal of Cardio-Thoracic Surgery, vol. 23, no. 3, pp. 292-298. https://doi.org/10.1016/s1010-7940(02)00817-5

Krag, S & Andreassen, TT 2003, 'Mechanical properties of the human lens capsule', Prog. Retin. Eye Res., vol. 22, no. 6, pp. 749-767.

Krag, S & Andreassen, TT 2003, 'Mechanical properties of the human lens capsule (review)', Progress in Retinal and Eye Research, no. 22, pp. 749-797.

Krag, S & Andreassen, TT 2003, 'Mechanical properties of the human posterior lens capsule', Invest. Ophthalmol. Vis. Sci., vol. 44, no. 2, pp. 691-696.

Krag, S & Andreassen, TT 2003, 'Mechanical properties of the human posterior lens capsule', Invest. Ophthalmol. Vis. Sci., no. 44, pp. 691-696.

Munkholm, LJ & Schjønning, P 2003, Mechanical subsoiling: Mitigation of recompaction by light traffic and on-land ploughing. in J Tullberg (ed.), Proceedings of the 16th International Conference of the International Soil Tillage Research Organization, ISTRO 2003. <Forlag uden navn>, pp. 813-819, Mechanical subsoiling, 18/12/2010.

Hernanz, R, Alonso, MJ, Briones, AM, Simonsen, U & Salaices, M 2003, 'Mechanisms involved in the early increase of serotonin contraction evoked by endotoxin in rat middle cerebral arteries', Br. J. Pharmacol., no. 140, pp. 671-680.

Fernandes, ES, Passos, GF, Campos, MM, Araújo, JGVC, Pesquero, JL, Avelllar, MC, Teixeira, MM & Calixto, JB 2003, 'Mechanisms underlying the modulatory action of platelet activating factor (PAF) on the upregulation of kinin B ₁ receptors in the rat paw', British Journal of Pharmacology, vol. 139, no. 5, pp. 973-981. https://doi.org/10.1038/sj.bjp.0705314

Vork, JC & Kristensen, IB 2003, 'Meckel's diverticulum and intestinal obstruction - report of a fatal case', Forensic Science International, vol. 138, pp. 114-115.

Vork, JC & Kristensen, IB 2003, 'Meckel's diverticulum and intestinal obstruction--report of a fatal case', Forensic Science International, vol. 138, no. 1-3, pp. 114-5.

Sørensen, KE & Hjortdal, VE 2003, 'Medfødte hjertesygdomme', Månedskrift for Praktisk Lægegerning, vol. 81, pp. 51-65.

Thøgersen, J 2003, 'Media attention and the market for "green" consumer products', Paper presented at GIN2003: Innovating for Sustainability, 11th International Conference - The Greening of Industry Network, San Francisco, USA, 12/10/2003 - 15/10/2003.

Perho, I 2003, Medicine and the Qur’an. in JD McAuliffe (ed.), Encyclopaedia of The Qur’an. vol. 3, Brill, Leiden, pp. 349-367.

Bonderup, G 2003, 'Medicinhistoriske Fortællinger', Bibliotek for Læger, vol. 4, no. 195, pp. 245-251.

Thorsager-Trier, X & Normander, B 2003, 'Medicinrester på vej mod grundvandet', Global Oekologi, vol. 5, pp. 20-22.

Larsen, KK 2003, Medicinsk leksikon. in J Hillingsø (ed.), Øjenvidnerne. Ni læger uden grænser fortæller.. 1 edn, FADL's Forlag, Copenhagen, pp. 353-361.

Displaying results 327851 to 327900 out of 389647

6558

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen