Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Kristensen, EF, Kofman, PD & Daugbjerg Jensen, P 2003, 'Counter pressure on ventilation of different types of wood chip and chunkwood', Biomass & Bioenergy, vol. 25, no. 4, pp. 399-408.

Andersen, PH & Chao, P 2003, 'Country-of-Origin Effects in Global Industrial Sourcing: Toward an Integrated Framework', Management International Review, vol. 43, no. 4, pp. 339-361. <http://www.baser.dk/login?url=http://proquest.umi.com/pqdlink?did=549173741&sid=1&Fmt=4&clientId=53681&RQT=309&VName=PQD>

Ruden, TA, Helgaker, T, Jørgensen, P & Olsen, J 2003, 'Coupled-cluster connected-quadruples corrections to atomization energies', Chemical Physics Letters, vol. 371, pp. 62-67.

Risgaard-Petersen, N 2003, 'Coupled nitrification-denitrification in autotrophic and heterotrophic estuarine sediments: On the influence of benthic microalgae', Limnology and Oceanography, vol. 48, no. 1, pp. 93-105.

Van Roosbroeck, J, De Witte, H, Gorska, M, Huyse, M, Kruglov, K, Van de Vel, K, Van Duppen, P, Franchoo, S, Cederkall, J, Fedoseyev, VN, Fynbo, H, Georg, U, Jonsson, O, Köster, U, Weissman, L, Mueller, WF, Mishin, VI, Fedorov, D, Walters, WB, Smirnova, NA, Van Dyck, A, De Maesschalck, A & Heyde, K 2003, 'Coupling a proton and a neutron to the semidoubly magic nucleus ⁶⁸Ni: A study of ⁷⁰Cu via the β decay of ⁷⁰Ni and ⁷⁰Cu', Physical Review C (Nuclear Physics), vol. 69, pp. 012703-1-11.

Strand, J 2003, 'Coupling marine monitoring and risk assessment by integrating exposure, bioaccumulation and effect studies: A case study using the contamination of organotin compounds in the Danish marine environment'. <http://hdl.handle.net/1800/571>

Petersen, JS, Bregenholt, S, Apostolopolous, V, Homann, D, Wolfe, T, Hughes, A, De Jongh, K, Dyrberg, TB, Von Herrath, MG & Wang, M 2003, 'Coupling of oral human or porcine insulin to the B subunit of cholera toxin (CTB) overcomes critical antigenic differences for prevention of type I diabetes', Clinical and Experimental Immunology, vol. 134, no. 1, pp. 38-45.

Ratzer, AV, Wells, LM , Lassen, HM , Laursen, M, Qvortrup, JF, Stissing, MS , Westergaard, M , Christensen, S , Jensen, K, Aalst, WMPVD (ed.) & Best, E (ed.) 2003, CPN Tools for Editing, Simulating, and Analysing Coloured Petri Nets. in WVD Aalst & E Best (eds), Applications and Theory of Petri Nets 2003. vol. 2679, Springer, Lecture Notes in Computer Science, vol. 2679, pp. 450-462, International Conference on Applications and Theory of Petri Nets. ICATPN 2003, Eindhoven, Netherlands, 23/06/2003.

Damian, D & Danvy, O 2003, 'CPS Transformation of Flow Information, Part II: Administrative Reductions', Journal of Functional Programming, vol. 13, no. 5, pp. 925-933. https://doi.org/10.1017/S0956796803004702

Jensen, HM 2003, 'Crack initiation and growth in brittle bonds', Engineering Fracture Mechanics, vol. 70, no. 13, pp. 1611-1621. https://doi.org/10.1016/S0013-7944(02)00200-X

Govindarajan, S, Agarwal, PK & Arge, LA 2003, CRB-Tree: An Efficient Indexing Scheme for Range-Aggregate Queries. in D Calvanese, M Lenzerini & R Motwani (eds), Database Theory - ICDT 2003: 9th International Conference Siena, Italy, January 8-10, 2003 Proceedings. Springer, Lecture Notes in Computer Science, vol. 2572, pp. 143-157, International Conference on Database Theory, Siena, Italy, 08/01/2003. https://doi.org/10.1007/3-540-36285-1_10

Birkedal, R & Gesser, H 2003, 'Creatine kinase and mitochondrial respiration in hearts of trout, cod and freshwater turtle', Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology, vol. 173, pp. 493-499.

Gulløv, E 2003, Creating a natural place for children: An ethnographic study of Danish kondergartens. in Children's places: cross-cultural perspectives. 1. udgave edn, Routledge, London.

Markham, A 2003, Creating Lit Reviews as Arguments: A Practical Guide. <http://www.markham.internetinquiry.org/2014/07/creatinglitreviewsasarguments/>

Weyer, G , Gunnlaugsson, HP, Dietrich, M, Fanciulli, M, Bharuth-Ram, K, Sielmann, R & nullISOLDECollaboration, T 2003, 'Creation and annealing of defect structures in silicon-based semiconductors during and after implantations at 77-500 K', Nucl. Instr. Meth. B, vol. 206, pp. 90-94.

Jensen, HS 2003, Crisis, Kuhn, Fuller.

Niekerk, KVK 2003, Critical Realism. in Encyclopedia of Science and Religion. vol. 1, Mac Millan Reference, New York, pp. 190-193.

Niekerk, KVK 2003, 'Critical Realism in Science and Theology', Omega - Indian Journal of Science and Religion, vol. 2, no. 2, pp. 89-100.

Mølgaard, JP, Grant, R & Kristensen, IS 2003, 'Crop production and nitrate leaching from grassland converted to cereal cropping', DARCOF enews, no. 4. <http://www.darcof.dk/enews/dec03/dinog.html>

Olesen, JE , Askegaard, M & Rasmussen, IA 2003, Crop production and N leaching in arable organic farming. in O Niemeläinen & M Topi-Hulmi (eds), Nordic Agriculture in Global Perspective: Proceedings of NJF's 22nd Congress . pp. 195-195.

Alexander, V 2003, Cross-Cultural Encounters in Amit Chaudhuri's Afternoon Raag and Yasmine Gooneratne's A Change of Skies. in The Politics of English as a World Language. New Horizons in Postcolonial Cultural Studies. Ed. Christian Mair. Brill | Rodopi, pp. 375-383, The Politics of English as a World Language. New Horizons in Postcolonial Cultural Studies, Freiburg i.Br., Germany, 17/12/2010.

Vestergaard, J 2003, Cross-Cultural Student/Business Cooperative Education: The Denmark Project. in Proceedings of the International Business and Economy Conference 2003. International Business and Economy Conference, San Francisco, USA, 09/01/2003.

Best, CT, Halle, P, Faber, A & Bohn, O-S 2003, Cross-language perception of non-native vowels. in International Conference of Phonetic Sciences. pp. 2889-2892, International Conference of Phonetic Sciences, Barcelona, Spain, 17/12/2010.

Berthelot, F, Martinat-Botte, F, Terqui, M & Vajta, G 2003, 'Cryopreservation of porcine embryos: state of the art', Livestock Production Science, vol. 83, no. 1, pp. 73-83. https://doi.org/10.1016/S0301-6226(03)00038-1

Enemark, HL 2003, 'Cryptosporidiosis a common cause of diarrhoea in calves', Proceedings of the Annual Meeting of the Association of Danish cattle practitioners, 30/10/2003.

Enemark, HL, Ahrens, P, Bille-Hansen, V, Heegaard, PMH, Vigre, H, Thamsborg, SM & Lind, P 2003, 'Cryptosporidium parvum: infectivity and pathogenicity of the 'porcine' genotype', Parasitology, vol. 126, no. Pt 5, pp. 407-416. https://doi.org/10.1017/s0031182003003032

Enemark, HL 2003, Cryptosporidium parvum. in Annual report on zoonoses in Denmark 2002. Ministry of Food, Agriculture and Fisheries .

Enemark, HL 2003, Cryptosporidium research at the Danish Veterinary Institute – new techniques and results. in Annual report 2002. Danish Veterinary Institute.

Rasmussen, SE , Grundvig, S, Lundtoft, B & Fogh, F 2003, 'Crystal data for CaNa-iminodisulfonat, trihydrat', Powder Diffraction, vol. 18, pp. 0-0.

Lorentzen, E, Pohl, E, Zwart, P, Stark, EA, Russell, RB, Knura, T, Hensel, R & Siebers, B 2003, 'Crystal structure of an archaeal class I aldolase and the evolution of (betaalpha)8 barrel proteins', J. Biol. Chem., vol. 278, no. 47, pp. 47253-47260.

Hartmann, R , Justesen, J, Sarkar, SN, Sen, GC & Yee, VC 2003, 'Crystal structure of the 2′-specific and double-stranded RNA-activated interferon-induced antiviral protein 2′-5′-oligoadenylate synthetase', Mol. Cell., vol. 12, pp. 1173-1185.

Boesen, T, Pavitt, GD & Andersen, GR 2003, 'Crystal structure of the catalytic domain of the initiation factor 2B epsilon subunit from saccharomyces cerevisiae', Translation 2003, United Kingdom, 03/07/2003 - 04/07/2003.

Grégoire, V, Levendag, P, Ang, KK, Bernier, J, Braaksma, M, Budach, V, Chao, C, Coche, E, Cooper, JS, Cosnard, G, Eisbruch, A, El-Sayed, S, Emami, B, Grau, C, Hamoir, M, Lee, N, Maingon, P, Muller, K & Reychler, H 2003, 'CT-based delineation of lymph node levels and related CTVs in the node-negative neck: DAHANCA, EORTC, GORTEC, NCIC, RTOG consensus guidelines', Radiotherapy & Oncology, vol. 69, no. 3, pp. 227-236. https://doi.org/10.1016/j.radonc.2003.09.011

Grégoire, V, Levendag, P, Ang, KK, Bernier, J, Braaksma, M, Budach, V, Chao, C, Coche, E, Cooper, JS, Cosnard, G, Eisbruch, A, El-Sayed, S, Emami, B, Grau, C, Hamoir, M, Lee, N, Maingon, P, Muller, K & Reychler, H 2003, 'CT-based delineation of lymph node levels and related CTVs in the node-negative neck: DAHANCA, EORTC, GORTEC, NCIC,RTOG consensus guidelines', Radiotherapy & Oncology, vol. 69, no. 3, pp. 227-36.

Pedersen, BG 2003, 'CT Colonography - a Danish Perspective'.

Tsing, A 2003, Cultivating the Wild. in C Zerner (ed.), Culture and the Question of Rights. Duke University Press, pp. 24-55.

Ravn, B (ed.) 2003, Cultural and Political Divergences in Approaches to Cooperation between Home, School and Local Society in Europe. in School, family, and community partnerships in a world of difference and changes. Gdansk University.

Nielsen, HK 2003, 'Cultural Policy and Evaluation of Quality', The International Journal of Cultural Policy, vol. 9, no. 3.

Gudmundsson, G 2003, Cultural Policy in Iceland. in The Nordic Cultural Model. Klim, Aarhus.

Lindenskov, L 2003, Cultural Understanding. in Adults Learning Mathematics Across Borders: A Grundtvig project of Belgium, Denmark, the Netherlands and Norway. Cinop, Centrum voor innovatie van opleidingen, 's Hertogenbosch, the Netherlands, pp. 23-30.

Rømer, JL , Kelsen, J , Dahl, R & Hoffmann, HJ 2003, 'Culture of Regulatory T-cell Lines from Bronchial Mucosa', Scandinavian Journal of Immunology, vol. 58, no. 2, I04, pp. 48.

Mustard, R, Baker, T, Williams, PW, Ulrich, T, Mernagh, T, Ryan, CG, Van Achterbergh, E & Adshead, N 2003, 'Cu-rich brines at the Osborne and Starra deposits: Implications for immiscibility in Fe-oxide Cu-Au systems', Transactions of the Institution of Mining and Metallurgy, Section B: Applied Earth Science, vol. 112, no. 2 AUG, pp. B189-B191.

Cook, RL & ØStergaard, L 2003, 'Current Issues in Screening for Chlamydia trachomatis', Current Infectious Disease Reports, vol. 5, no. 2, pp. 153-158.

Almeida, I 2003, Curso de Metodología de la investigación.

Lüneborg, JL & Nielsen, JF 2003, 'Customer-focused technology and performance in small and large banks', European Management Journal, vol. 21, no. 2, pp. 258-269. https://doi.org/10.1016/S0263-2373(03)00020-3

Lysgaard, J 2003 'CVRPSEP: A package of separation routines for the Capacitated Vehicle Routing Problem'.

Bryld, M & Markussen, R 2003, Cyberkulturer og rekonfigurationer. Samfundslitteratur, Gylling.

Lykke, N, Markussen, R , Olesen, F & Haraway, D (ed.) 2003, Cyborgs, Coyotes and Dogs: A Kinship of Feminist Figurations and: There are always more things going on than you thought!: Methodologies as Thinking Technologies. in The Donna Haraway Reader. Frank Cass Publishers, New York, pp. 321-342.

Chen, S, Glawischnig, E, Jorgensen, K, Naur, P, Jørgensen, B, Olsen, CE, Hansen, CH, Rasmussen, H, Pickett, JA & Halkier, BA 2003, 'CYP79F1 and CYP79F2 have distinct functions in the biosynthesis of aliphatic glucosinolates in Arabidopsis', Plant Journal, vol. 33, no. 5, pp. 923-938. https://doi.org/10.1046/j.1365-313X.2003.01679.x

Naur, P, Petersen, BL, Bak, S, Olsen, CE & Halkier, BA 2003, 'CYP83A1 and CYP83B1, Two Non-Redundant Cytochrome P450 Enzymes Metabolizing Oximes in the Biosynthesis of Glucosinolates in Arabidopsis thaliana', Plant Physiology, vol. 133, pp. 63-72. https://doi.org/10.1104/pp.102.019240

Displaying results 324901 to 324950 out of 390544

6495

6496

6497