Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Thuesen, L , Nørgaard, BL, Søndergaard, MM, Kragholm, KH, Christensen, MK, Tang, M & Jakobsen, CJ 2025, 'Computed tomography angiography in chronic coronary syndrome: Association to catheterization laboratory activity and survival', American Heart Journal Plus: Cardiology Research and Practice, vol. 57, 100582. https://doi.org/10.1016/j.ahjo.2025.100582

Caeli, EN , Pedersen, CS, Zambach, S, Misfeldt, M & Bundsgaard, J 2025, Computer Science Education in Secondary School: A Real-Life Approach to Teaching Students Technology-Critical Aspects of AI. in ITiCSE 2025 - Proceedings of the 30th ACM Conference on Innovation and Technology in Computer Science Education: Proceedings of the 30th ACM Conference on Innovation and Technology in Computer Science Education V. 2. pp. 727-728. https://doi.org/10.1145/3724389.3731271

Bjerge, K 2025, 'Computer Vision and Deep Learning for Insect Monitoring', PhD, Aarhus University.

Mortensen, JPB & Bizzoni, Y 2025, 'Computing Mark’s Genre', Religions , vol. 16, no. 12, 1568. https://doi.org/10.3390/rel16121568

Fathollahi, A & Beatini, V 2025, 'Concept Design of Variable Density, Flat Cut Active Bending Shells', International Symposium on Architecture, Technology, and Innovation , Izmir, Turkey, 03/10/2024 - 04/10/2024.

Brückner, M , Lysgaard, JA, Elf, N & Reid, A 2025, 'Conceptions and dimensions of quality in environmental and sustainability education', Environmental Education Research. https://doi.org/10.1080/13504622.2025.2604767

Reuter, K, Latham, AJ & Varga, S 2025, 'Concept(s) of Health: Lifestyle at the Heart of Modern Health', Erkenntnis. https://doi.org/10.1007/s10670-025-00971-3

Varga, S , Latham, AJ & Machery, E 2025, 'Concepts of Health and Disease: Insights from Experimental Philosophy of Medicine', Synthese, vol. 206, 219. https://doi.org/10.1007/s11229-025-05280-4

Haubrock, PJ, Parker, B, Błońska, D, Briski, E, Everts, T, Fernandez, RD, Kouba, A, Kourantidou, M, Kurtul, I, Mammola, S, Musolin, DL, Nuñez, MA, Olden, JD, Rasmussen, JJ, Renault, D, Russell, JC, Sousa, R, Tarkan, AS & Britton, JR 2025, 'Conceptual and ethical considerations in invasion science', BioScience, vol. 75, no. 4, pp. 317-330. https://doi.org/10.1093/biosci/biae138

Rafner, J 2025, Conceptual Co-creativity. in J Katz-Buonincontro & T Kettler (eds), The Oxford Handbook of Creativity and Education. Oxford University Press, pp. 817-846. https://doi.org/10.1093/oxfordhb/9780197698181.013.0043

Lysgaard, JA, Frydensbjerg Elf, N, Steffensen, T, Laugesen, MH-L, Brückner, M & Reid, A 2025, 'Conceptualising and (re)modelling ESE teaching quality', Paper presented at ECER 2025, 09/09/2025 - 12/09/2025.

Hald, AN , Enemark, U, Mwale, ML & Burau, V 2025, 'Conceptualising the primary health care workforce: A meta-narrative-inspired review of stakeholder perspectives in Denmark', Health Policy, vol. 162, 105474. https://doi.org/10.1016/j.healthpol.2025.105474

Camia, C & Bohn, A 2025, Conceptualizing Cultural Life Scripts as Master Narratives: An Underestimated Tool to Explore Life Stories. in C Camia & A Bohn (eds), Autobiographical Memory and the Life Story: New Perspectives on Narrative Identity. Cambridge University Press.

Valdes, H, Correa, C, Suarez, C, Laurens Arredondo, LA, Hurtado Espinosa, MF, Vera-Puerto, IL, Zagal, M & Arias, CA 2025, 'Conceptual model for facultative symbiosis between sustainable construction and nature-based solutions in the training of engineers in Chile', International Journal of Sustainability in Higher Education, vol. 26, no. 4, pp. 773-803. https://doi.org/10.1108/IJSHE-06-2024-0378

Fage-Butler, A , Nielsen, KH & Ledderer, LK 2025, Conclusion. in Science communication and trust. Palgrave Macmillan, pp. 493-509. https://doi.org/10.1007/978-981-96-1289-5_24

Hinnebusch, R & Valbjørn, M (eds) 2025, Conclusion: exposing patterns of sectarianization in Syria. in R Hinnebusch & M Valbjørn (eds), Sectarianism and Civil War in Syria. Routledge, London, pp. 367-388. <https://www.routledge.com/Sectarianism-and-Civil-War-in-Syria/Hinnebusch-Valbjorn/p/book/9781032903828>

Borup, J, Plank, K, Jacobsen, K & Härkonen, M 2025, Conclusion. in J Borup, M Härkönen & KA Jacobsen (eds), Buddhism in the Nordic Countries. Routledge, London, pp. 202-206. https://doi.org/10.4324/9781003529767-10

Nielsen, JI, Heiselberg, L & Walther, BK 2025, Conclusion. in L Heiselberg, BK Walther, JI Nielsen & R Rønlev (eds), Seriality in the Streaming Era. Routledge, London, pp. 151-159. https://doi.org/10.4324/9781003539735-6

Landorff, L, Knudsen, TB & Jørgensen, KE 2025, Conclusion and Wider Perspectives. in KE Jørgensen, TB Knudsen & L Landorff (eds), Europe's World: Policy Paradigms, Strategic Thinking and the Anti-Liberal Challenge. Bristol University Press, Bristol, pp. 274-285. https://doi.org/10.56687/9781529243642-015

Multicenter Registry for Hyperbaric Oxygen Treatment 2025, 'Conclusions from Kalaw et al. paper are flawed and do a disservice to patients who present early after central retinal artery occlusion and may benefit from hyperbaric oxygen treatment', Retina. https://doi.org/10.1097/IAE.0000000000004453

REDWOOD-HCM, SEQUOIA-HCM, and FOREST-HCM Investigators 2025, 'Concomitant Aficamten and Disopyramide in Symptomatic Obstructive Hypertrophic Cardiomyopathy', JACC: Heart Failure. https://doi.org/10.1016/j.jchf.2025.03.008

Halland, I & Solbakken, K 2025, 'Concurrencies: Mutating Methods and Latent Leverage', Metode, vol. 3, no. Currents. <https://metode.rom.no/articles/essay/concurrencies-mutating-methods-and-latent-leverage>

Ellegaard-Jensen, L , Carvalho, PN, Anwar, MZ, Schostag, MD, Bester, K & Jacobsen, CS 2025, 'Concurrent stimulation of diflufenican biodegradation and changes in the active microbiome in gravel revealed by Total RNA', Microbiology Spectrum, vol. 13, no. 10, pp. 1-17. https://doi.org/10.1128/spectrum.00164-25

Dallant, J & Iacono, J 2025, 'Conditional Lower Bounds for Dynamic Geometric Measure Problems', ACM Transactions on Algorithms, vol. 21, no. 2, 23. https://doi.org/10.1145/3727878

Yang, G, Jin, J, Wang, K , Baad-Hansen, L, Liu, H, Cao, Y, Xie, QF & Svensson, P 2025, 'Conditioned Pain Modulation Differences in Central and Peripheral Burning Mouth Syndrome (BMS) Patients', Journal of Oral Rehabilitation, vol. 52, no. 4, pp. 443-452. https://doi.org/10.1111/joor.13876

Duarte, M, Magnolfi, L, Quint, D, Sølvsten, M & Sullivan, C 2025 'Conduct and Scale Economies: Evaluating Tariffs in the US Automobile Market'.

Hussein, A, Zhang, X, Pedersen, BP & Stokes, DL 2025, 'Conduction pathway for potassium through the Escherichia coli pump KdpFABC', eLife, vol. 14. https://doi.org/10.7554/eLife.107397

Schulze, R, Klingler, S, Biel, P, Spin-Neto, R, Berkhout, E, Drage, N, Jäghagen, EL, Semper-Hogg, W, Różyło-Kalinowska, I, Hol, C & Arvidsson, L 2025, 'Cone beam computed tomography – Frequency and exposure settings at University (Dental) hospitals in Central and Northern Europe', European Journal of Radiology, vol. 183, 111886. https://doi.org/10.1016/j.ejrad.2024.111886

Klucznik, KA , Ravkilde, T , Skouboe, S , Møller, DS , Hokland, S, Keall, P, Buus, S, Bentzen, L & Poulsen, PR 2025, 'Cone-beam CT-based estimations of prostate motion and dose distortion during radiotherapy', Physics and Imaging in Radiation Oncology, vol. 35, 100798. https://doi.org/10.1016/j.phro.2025.100798

Stehr, M & Kiderlen, M 2025, 'Confidence intervals for Newton–Cotes quadratures based on stationary point processes', Statistical Methods & Applications, vol. 34, no. 1, pp. 39-67. https://doi.org/10.1007/s10260-024-00773-x

Andreoli, L, Julsgaard, M & Schreiber, K 2025, 'Confidence in the Use of Immunomodulatory Medications During Pregnancy: How Can Patient and Clinician Experience Be Improved?', Journal of Rheumatology, vol. 52, no. 11, pp. 1078-1080. https://doi.org/10.3899/jrheum.2025-0941

Sevigny, EL, Potter, G, Wilkins, C, Barratt, MJ, Søgaard, TF, Hakkarainen, P, Grigg, J & Jauffret-Roustide, M 2025, 'Conflict and Social Control among Cannabis Growers', International Journal of Drug Policy, vol. 144, no. 3, 104292. https://doi.org/10.1016/j.drugpo.2023.104292

Pfiffelmann, J, Untilov, O, Thøgersen, J & Franck, R 2025, 'Conflicting Consumer Beliefs Influencing Eco-Innovation Adoption: Motives and Barriers for Accepting the Laser Marking of Organic Products', Psychology and Marketing, vol. 42, no. 3, pp. 866-891. https://doi.org/10.1002/mar.22156

Lauridsen, AGS, Rasmussen, AP , Klinkby, N & Andersen, LH 2025, 'Conformer specific photophysical properties of an analog of the green fluorescent protein chromophore anion', Physical Chemistry Chemical Physics, vol. 27, no. 34, pp. 17686-17691. https://doi.org/10.1039/d5cp02407b

Golgeci, I, Ritala, P, Arslan, A, McKenna, B & Ali, I 2025, 'Confronting and alleviating AI resistance in the workplace: An integrative review and a process framework', Human Resource Management Review, vol. 35, no. 2, 101075. https://doi.org/10.1016/j.hrmr.2024.101075

Sovacool, BK 2025, 'Confronting a “silent killer:” reducing vulnerability to extreme heat in urban West Africa with surface cooling technology and climate-resilient roofing', Climatic Change, vol. 178, no. 12, 212. https://doi.org/10.1007/s10584-025-04061-9, https://doi.org/10.1007/s10584-025-04061-9

Ravichandran, B, Henriksen, TB, Hjortdal, VE, Ostergaard, JR & Matthiesen, NB 2025, 'Congenital Heart Defects and Apgar Score at Birth, a Nationwide Study', Journal of the American Heart Association, vol. 14, no. 8, e038798. https://doi.org/10.1161/JAHA.124.038798

Gnech, M, ’t Hoen, L, Radmayr, C, Burgu, B, Bogaert, G, O'Kelly, F, Castagnetti, M, Quaedackers, J, Silay, MS, Kennedy, U, van Uitert, A, Skott, M, Pakkasjärvi, N, Bujons, A, Yuan, Y & Rawashdeh, YF 2025, 'Congenital Lower Urinary Tract Obstruction: Update and Summary of the European Association of Urology and European Society for Paediatric Urology Guidelines', European Urology Focus, vol. 11, no. 3, pp. 519-527. https://doi.org/10.1016/j.euf.2025.01.012

Karzoun, D 2025, 'Congenital Pulmonary Malformation, Pre- and Postnatal Diagnosis and Management', PhD, Aarhus University.

Østergaard, HC, Nielsen, LH & Knudsen, A-K 2025, 'Conjoined thoracopagus twins – a case report', Danish Journal of Obstetrics and Gynaecology. https://doi.org/10.56182/h3eeka78

493907 2025, 'Connecting the Army Organization: The Command Team and the Command Senior Enlisted Leader', PhD, Aarhus University. <https://politica.dk/fileadmin/politica/Dokumenter/Afhandlinger/rasmus_marquard_andresen.pdf>

Pasgaard, M, Breed, C, Engemann, K & Brom, P 2025, 'Connecting urban green infrastructure and environmental justice in South Africa: Integrating social access, ecology, and design', Journal of Political Ecology, vol. 32, no. 1, 6186. https://doi.org/10.2458/jpe.6213

Van Maldegem, M, Vohryzek, J, Atasoy, S, Alnagger, N, Cardone, P, Bonhomme, V, Vanhaudenhuyse, A, Demertzi, A, Jaquet, O, Bahri, MA, Nunez, P, Kringelbach, ML, Stamatakis, EA & Luppi, AI 2025, 'Connectome harmonic decomposition tracks the presence of disconnected consciousness during ketamine-induced unresponsiveness', British Journal of Anaesthesia, vol. 134, no. 4, pp. 1088-1104. https://doi.org/10.1016/j.bja.2024.12.036

Bengtson, A & Munch, LA 2025, 'Consensual Discrimination', Philosophical Quarterly, vol. 75, no. 3, pp. 878-899. https://doi.org/10.1093/pq/pqae039

Arlien-Søborg, MC, Radovick, S, Boguszewski, MCS, Bidlingmaier, M, Johannsson, G, Grimberg, A, Ho, KKY, Biller, BMK, Choong, CS, Hoffman, AR, Backeljauw, P, Boguszewski, CL, Bollerslev, J, Brue, T, Chanson, P, Christ, E, Cianfarani, S, Clayton, PE, Cohen, P, Dauber, A, Fleseriu, M, Gebauer, J, Giustina, A, Higham, CE, Horikawa, R, Höybye, C, Juul, A, Lodish, M, Luo, X, Mauras, N, Miller, KK, Melmed, S, Neggers, SJCMM, Karavitaki, N, Rosenfeld, R, Ross, R, Savendahl, L, Schilbach, K, Collett-Solberg, PF, Strasburger, CJ, Tritos, NA, van Santen, HM, Yuen, KCJ & Jørgensen, JOL 2025, 'Consensus and controversies about diagnosing GH deficiency: a Delphi survey by the GH research society', Pituitary, vol. 28, no. 57, 57. https://doi.org/10.1007/s11102-025-01526-z

Emmanuel, A, Krogh, K, Perrouin-Verbe, B, Krassiukov, A, Koch, S, Mosiello, G, Bazzocchi, G, Christensen, P, Rodriguez, G & Brugaletta, C 2025, 'Consensus on Safe Initiation and Monitoring of Transanal Irrigation to Optimize Adherence With Therapy', Neurogastroenterology and Motility, vol. 37, no. 5, e15011. https://doi.org/10.1111/nmo.15011

Zhu, T , Marschall, H , Hansen, KE, Horne, AW, Saraswat, L, Zondervan, KT, Missmer, SA, Hummelshoj, L, Bokor, A, Østrup, CS, Melgaard, A & Rytter, D 2025, 'Consensus on Symptom Selection for Endometriosis Questionnaires: A Modified e-Delphi Study', BJOG: An International Journal of Obstetrics and Gynaecology, vol. 132, no. 5, pp. 656-662. https://doi.org/10.1111/1471-0528.18066

Bjerklund Johansen, TE, Bahrs, C, Bruyere, F, Cai, T, Joseph, A, Köves, B, Livermore, DM, Oliva, A, Soriano, A, Wagenlehner, F, Zahl, PH, Etienne, M, Batura, D, Graugaard-Jensen, C, Kaye, KS, Kranz, J, Lanzafame, M, Medina-Polo, J, Membrillo de Novales, FJ, Naber, KG, Nickel, JC, Schneidewind, L, Tandoğdu, Z & cUTI Consensus Group 2025, 'Consensus position statement on advancing the classification of patients and tests of cure in studies of antibiotic treatment of complicated urinary tract infections', The Lancet Infectious Diseases, vol. 25, no. 10, pp. e605-e618. https://doi.org/10.1016/S1473-3099(25)00142-2

Punwani, S, Larson, PEZ, Laustsen, C, VanderMeulen, J, Ardenkjær-Larsen, JH, Autry, AW, Bankson, JA, Bernard, J, Bok, R, Bertelsen, LB, Che, J, Chen, AP, Chowdhury, R, Comment, A, Cunningham, CH, Dang, D, Gallagher, FA, Gaunt, A, Gong, Y, Gordon, JW, Grimmer, A, Grist, J, Hansen, ESS, Lerche, MH, Hesketh, RL, Hoevener, J-B, Hsieh, C-Y, Keshari, KR, Kozerke, S, Lanz, T, Mayer, D, McLean, M, Park, JM, Slater, J, Tyler, D, Vanderheyden, J-L, von Morze, C, Zaccagna, F, Zaha, V, Xu, D, Vigneron, D & HP 13C MRI Consensus Group 2025, 'Consensus recommendations for hyperpolarized [1-13C]pyruvate MRI multi-center human studies', Magnetic Resonance in Medicine, vol. 94, no. 4, pp. 1386-1400. https://doi.org/10.1002/mrm.30570

Darfelt, IS , Neergaard, MA, Klepstad, P, Hästbacka, J, Thorarensen, G, Robertson, AC, Valsø, Å, Jensen, HI, Segerlantz, M, Lehto, JT, Malmgren, J, Møller, MH & Nielsen, AH 2025, 'Consensus statements on end-of-life care in ICU: A Scandinavian multidisciplinary Delphi study', Acta Anaesthesiologica Scandinavica, vol. 69, no. 4, e70015. https://doi.org/10.1111/aas.70015

Displaying results 7401 to 7450 out of 391130

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Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen