Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Pegoraro, S, Veronelli, L, Frisco, F, Brattico, E, Romano, D, Daini, R & Tosi, G 2026, 'Effectiveness of Cognitive Interventions in Healthy Aging: A Systematic Review and Bayesian Meta-analysis', Neuroscience and Biobehavioral Reviews, vol. 183, 106571. https://doi.org/10.1016/j.neubiorev.2026.106571

Jensen, EK, Lichtenstein, MB, Riper, H & Mathiasen, K 2026, 'Effectiveness of Guided Internet-Based Cognitive Behavioral Therapy for Adult Anxiety and Depression in Routine Care: An Observational Study', International Journal of Methods in Psychiatric Research, vol. 35, no. 1, e70058. https://doi.org/10.1002/mpr.70058

Andersen, KS, Hansen, AP, Pedersen, P , Langagergaard, V , Nielsen, CV, Løvschall, C & Pilegaard, MS 2026, 'Effectiveness of multidisciplinary biopsychosocial rehabilitation including workplace interventions for employees on sick leave due to low back pain: A systematic review', Work (Reading, Mass.), pp. 10519815261423506. https://doi.org/10.1177/10519815261423506

Shrestha, R, Paudel, S, Adhikari, TB , Khatri, B, Adhikari, S, Neupane, D, Vaidya, A, Kallestrup, P & Rijal, A 2026, 'Effectiveness of physical activity promotion interventions in low-income and middle-income countries: a systematic review and meta-analysis', British Journal of Sports Medicine, vol. 60, no. 4, pp. 286-299. https://doi.org/10.1136/bjsports-2025-110035

Kristensen, FPB , Christensen, DH, Callaghan, BC, Nielsen, JS , Andersen, H , Sørensen, HT & Thomsen, RW 2026, 'Effectiveness of Sodium-Glucose Cotransporter-2 Inhibitors Versus Glucagon-like Peptide-1 Receptor Agonists on Diabetic Foot Disease: An Emulated Target Trial', Annals of Internal Medicine. https://doi.org/10.7326/ANNALS-25-01262

Masri, A, Judge, DP, Ruberg, FL, Gillmore, JD, Grodin, JL, Obici, L, Maurer, MS, Fontana, M, Poulsen, SH, van der Meer, P, Cheng, RK, Cuddy, SAM, Ambardekar, AV, Xiong, K, Cao, X, Murtagh, G, Siddhanti, S, Castaño, A, Tamby, J-F, Fox, JC, Alexander, KM & Witteles, R 2026, 'Effect of Acoramidis on Recurrent and Cumulative Cardiovascular Outcomes in ATTR-CM: Exploratory Analysis From ATTRibute-CM', Journal of the American College of Cardiology, vol. 87, no. 5, pp. 490-501. https://doi.org/10.1016/j.jacc.2025.09.013

Martins, JT , Peixoto, L , Giannini-Kurina, F , Han, E & Rasmussen, J 2026, 'Effect of additional inoculation on in situ biomass, nodule formation and N₂ fixation in soybean under Northern European conditions', Plant and Soil, vol. 518, no. 1, pp. 197-210. https://doi.org/10.1007/s11104-025-07988-1

Ovesen, SH , Skaarup, SH , Aagaard, R , Løfgren, B, Posth, S, Arvig, MD, Laursen, CB , Kirkegaard, H & Weile, J 2026, 'Effect of a point-of-care ultrasound-driven diagnostic pathway on diagnostic certainty in emergency department patients with dyspnea: a randomized controlled substudy', European journal of emergency medicine : official journal of the European Society for Emergency Medicine. https://doi.org/10.1097/MEJ.0000000000001331

Wang, Y, Dechesne, A, Franck, SL, Klümper, U, Wang, G & Smets, BF 2026, 'Effect of biofilm lifestyle caused by water matric potential on invasion of exogenous plasmid', ISME Communications, vol. 6, no. 1, ycag031. https://doi.org/10.1093/ismeco/ycag031

Højen, IB, Kristensen, MK, Bertelsen, VM , Tranberg, M, Petersen, LK, Andersen, B & Bor, P 2026, 'Effect of coriolus versicolor-based vaginal gel on clearance of human papillomavirus and cervical dysplasia - a scoping review', BMC Women's Health, vol. 26, no. 1, 71. https://doi.org/10.1186/s12905-025-04206-z

Vasdal, G, Kittelsen, KE, Janczak, AM, Moe, RO, Rodenburg, TB, Gebhardt-Henrich, SG, Riber, AB & Tahamtani, FM 2026, 'Effect of early litter access on plumage and fearfulness in commercial laying hen pullets', Applied Animal Behaviour Science, vol. 297, 106929. https://doi.org/10.1016/j.applanim.2026.106929

Thomsen, JPS , Poulsen, NA & Larsen, LB 2026, 'Effect of enzymatic dephosphorylation on in vitro digestion of purified bovine caseins', International Dairy Journal, vol. 172, 106426. https://doi.org/10.1016/j.idairyj.2025.106426

Pedersen, K, Kristensen, C, Mogensen, EH, Kvisgaard, LK, Holm, C & Larsen, LE 2026, 'Effect of Far-ultraviolet C irradiation on infection risk by airborne porcine reproductive and respiratory syndrome virus 1', Veterinary Microbiology, vol. 312, 110816. https://doi.org/10.1016/j.vetmic.2025.110816

Yafasova, A, Doi, SN, Thune, JJ, Nielsen, JC, Bruun, NE, Eiskjær, H, Hassager, C, Svendsen, JH, Høfsten, DE, Torp-Pedersen, C, Pehrson, S, Køber, L & Butt, JH 2026, 'Effect of implantable cardioverter-defibrillator in non-ischemic heart failure according to heart failure etiology: Extended follow-up of the DANISH trial', International Journal of Cardiology, vol. 443, 133969. https://doi.org/10.1016/j.ijcard.2025.133969

Iversen, AM, Hansen, MB, Kristensen, B & Ellermann-Eriksen, S 2026, 'Effect of individual feedback on hand hygiene compliance in nursing homes', Infection Prevention in Practice, vol. 8, no. 2, 100508. https://doi.org/10.1016/j.infpip.2026.100508

Feyera, T, Carrión-López, MJ, Gardan-Salmon, D, Hamard, A, Robert, F & Nørgaard, JV 2026, 'Effect of Scutellaria baicalensis supplementation on sow milk yield and litter growth performance in Danish sows', Translational Animal Science, vol. 10, txag009. https://doi.org/10.1093/tas/txag009

Souissi, A, Patti, F, Spelman, T, Chisari, C, Gargouri, A, John, N, Kermode, AG, Kalincik, T, Butzkueven, H, Sajedi, SA, Lechner-Scott, J, Roos, I, Laureys, G, Taylor, B, Alroughani, R, Khoury, SJ, Macdonell, R, Weinstock-Guttman, B, Havrdova, EK, Maimone, D, Reddel, S, Fabis-Pedrini, M, Willekens, B, Moghadasi, AN, Lalive, P, Lugaresi, A, Ozakbas, S, Solaro, C, Cárdenas-Robledo, S, Shaygannejad, V, Etemadifar, M, Boz, C, Eichau, S, Tomassini, V, Terzi, M, Prat, A, Habek, M, Blanco, Y, Altintas, A, Gerlach, O, Turkoglu, R, Buzzard, K, Skibina, O, Soysal, A, van der Walt, A, Hughes, S, van Pesch, V, Foschi, M, Surcinelli, A, Prevost, J, Ramo-Tello, C, McGuigan, C, Sa, MJ, Kuhle, J, Spitaleri, D, Singhal, B, Ampapa, R, de Gans, K, Petersen, T, Simu, M, Lapointe, E, Sanchez-Menoyo, JL, Gray, O, Garber, J, Aguera-Morales, E, Gross-Paju, K, Castillo-Triviño, T, Al-Asmi, A, Grigoriadis, N, Inshasi, J, Al-Harbi, T, Hardy, TA, Ramanathan, S, Cambron, M, Shuey, N, sempere, AP, Csepany, T, Treviño-Frenk, I, Rozsa, C, Cauchi, M, Karabudak, R, Mrabet, S & Gouider, R 2026, 'Effect of late-onset on multiple sclerosis phenotype and outcome: evidence from a multi-national registry', Journal of Neurology, vol. 273, no. 2, 114. https://doi.org/10.1007/s00415-026-13632-4

Myrup Holst, C , Esperon-Abril, I , Bryske Juhl, F , Jakobsgaard, JE , Kristiansen, JB , Vissing, K & Stevnsner, T 2026, 'Effect of prolonged voluntary wheel running on oxidative stress and defence mechanisms in cortex and hippocampus of healthy female rats', Experimental Physiology, vol. 111, no. 2, pp. 449-463. https://doi.org/10.1113/EP092815

Bomediano, MP, Plivelic, TS, Pedersen, JS, Galantini, L, Schillén, K & de Oliveira, MG 2026, 'Effect of sodium taurodeoxycholate on PEO-PPO-PEO triblock copolymer F127 with incorporated SNAP: Insights into micellization, gelation, and nitric oxide release', Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 730, 138969. https://doi.org/10.1016/j.colsurfa.2025.138969

Heltoft, P, Torp, T, Wold, AB, Edelenbos, M & Thomsen, MG 2026, 'Effect of temperatures strategies in the early storage period on weightloss, respiration rate and disease development in carrot (Daucus carota subsp. sativus)', Postharvest Biology and Technology, vol. 231, 113911. https://doi.org/10.1016/j.postharvbio.2025.113911

Mohammad-Rahimi, H, Ourang, SA, Nosrat, A, Spin-Neto, R & Pauwels, R 2026, 'Effect of Voxel Size on Cone-Beam Computed Tomography-Based Assessment of Root Canal Anatomy: A Systematic Review and Meta-Analysis', International Endodontic Journal, vol. 59, no. 4, pp. 574-589. https://doi.org/10.1111/iej.70092

Kristiansen, J , Kristensen, SD , Hvas, A-M, Ellingsgaard, H, Mohr, M, Grove, EL & Sjúrðarson, T 2026, 'Effects of Acute Exercise and 12-Week High-Intensity Interval Training on Inflammatory Biomarkers in Stable Coronary Artery Disease: A Randomized Controlled Trial', Journal of the American Heart Association, vol. 15, no. 4, e042256, pp. 1-15. https://doi.org/10.1161/JAHA.125.042256

Le Morvan de Sequeira, C, Bruguera, MB, Klos, B, Albertsen, N, Thoolen, S, Hagson, H, Van Ombergen, A, Enck, P & Mack, I 2026, 'Effects of a long-term isolated, confined and extreme environment in Antarctica on psychological well-being', Journal of Environmental Psychology, vol. 111, 102991. https://doi.org/10.1016/j.jenvp.2026.102991

Drocco, C, Fernandes, S, Xie, L, Devers, M, Förster, B, Martin-Laurent, F, Romdhane, S, Spor, A, Thiour-Mauprivez, C & Coors, A 2026, 'Effects of a sequential application of plant protection products on soil microbes and free-living nematodes in a field experiment', Environmental Toxicology and Chemistry, vol. 45, no. 2, pp. 351 - 362. https://doi.org/10.1093/etojnl/vgaf274

Fernandes, S, Xie, L, Drocco, C, van Gestel, CAM, Berg, MP, Holmstrup, M , Slotsbo, S, Förster, B & Coors, A 2026, 'Effects of a sequential application of three pesticides on soil microarthropods in a field study', Environmental Toxicology and Chemistry, vol. 45, no. 2, pp. 341-350. https://doi.org/10.1093/etojnl/vgaf252

Sternhagen Nielsen, AB, Folke, S, Larsen, D, Schmidt, BW, Gjelstrup, JW, Machon, HT, Roitmann, NC, Nyboe, L & Nissen, LR 2026, 'Effects of basic body awareness therapy on movement quality, arousal regulation, and bodily experience in Danish military veterans with severe PTSD symptoms', Journal of Bodywork and Movement Therapies, vol. 46, pp. 139-146. https://doi.org/10.1016/j.jbmt.2025.11.013

Xiao, T, Duque-Estrada, P, Larsen, ME, Petersen, IL, Jensen, PE, Larsen, LB & Poulsen, NA 2026, 'Effects of dehulling and boiling on protein digestibility in faba beans with high and low trypsin inhibitor activity', Food and Function, vol. 17, no. 1, pp. 304-314. https://doi.org/10.1039/d5fo03653d

Jørgensen, N , Chen, L , Thorup, VM , Weisbjerg, MR & Østergaard, S 2026, 'Effects of dietary fat supplementation on enteric methane mitigation and farm profitability: A case study of an organic and a conventional dairy herd', Livestock Science, vol. 303, 105856. https://doi.org/10.1016/j.livsci.2025.105856

Campitelli, RR, Guagnoni, IN, Petterson, LW, De Nucci, G & Wang, T 2026, 'Effects of endothelium-derived catecholamines on heart rate, blood pressure and gill ventilation in the rainbow trout', Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology, vol. 315, 111991. https://doi.org/10.1016/j.cbpa.2026.111991

Jeppesen, E, Su, H, Wang, H & Liu, Z 2026, 'Effects of Eutrophication and Warming on Lake Ecosystems', Global change biology, vol. 32, no. 1, e70715. https://doi.org/10.1111/gcb.70715

Hansen, SB 2026, 'Effects of exogenous glucocorticoid exposure on energy expenditure, substrate metabolism and circadian rhythms: A whole room indirect calorimetry study in healthy human subjects', PhD, Aarhus University.

Zhen, W, Zhang, X, Lin, Z, Gao, Y, Wang, Q, Yang, K, Guan, B, Li, K, Jeppesen, E, Liu, Z & Yu, J 2026, 'Effects of Herbivorous Fish on Competition and Growth of Canopy-Forming and Meadow-Forming Submerged Macrophytes: Implications for Lake Restoration', Water (Switzerland), vol. 18, no. 1, 28. https://doi.org/10.3390/w18010028

Ludvig, EA, McMillan, N, Pisklak, JM, Simonsen, N, Mason, A, Long, J, Spetch, ML & Madan, CR 2026, 'Effects of initial experiences on risky choice', Quarterly journal of experimental psychology , pp. 17470218261432610. https://doi.org/10.1177/17470218261432610

Svensson, NS , Volqvartz, T , Vestergaard, AL , Vestergaard, ET , Larsen, A & Bor, P 2026, 'Effects of Maternal Vitamin D Supplementation on Childhood Health', Endocrine Reviews, vol. 46, no. 6, pp. 922-953. https://doi.org/10.1210/endrev/bnaf034

Hansen, SB , Frederiksen, JSS , Lodberg, A , Poulsen, MF , Andersen, CBF, Oster, H, Jessen, N & Jørgensen, JOL 2026, 'Effects of Prednisolone Administration on Clock Gene Expression and Indices of Circadian Rhythms in Healthy Human Males', Journal of Clinical Endocrinology and Metabolism, vol. 111, no. 4, pp. 993-1003. https://doi.org/10.1210/clinem/dgaf564

Coutant, MP , Hedemann, MS , Pedersen, LJ & Larsen, MLV 2026, 'Effects of single extensification factors on the occurrence of tail and ear biting, pen fouling and stomach ulcers in finisher pigs raised in conventional indoor husbandry', Livestock Science, vol. 307, no. 105934, 105934. https://doi.org/10.1016/j.livsci.2026.105934

Peng, R, Räisänen, SE, Rauch, R, Rytz, A, Zhang, Y, Ma, X, Barrientos-Blanco, MA, Wang, K, Zeng, Z, Yip, WT, Bobkov, M, Islam, MZ, Soussan, E, Kreuzer, M & Niu, M 2026, 'Effects of tannins and additional rumen-protected protein on nitrate responses in dairy cows: Lactational performance, enteric methane emissions, nitrogen utilization, and blood metabolites', Journal of Dairy Science, vol. 109, no. 1, pp. 372-389. https://doi.org/10.3168/jds.2025-26786

Clubley, CH, Firth, LB & Knights, AM 2026, 'Effects of topographic complexity on space-use by a key intertidal grazer in artificial environments', Movement Ecology, vol. 14, no. 1, 12. https://doi.org/10.1186/s40462-026-00629-x

Kristensen, M 2026, 'Effektivitetsvurdering af 26-KX-FL-09', No. 2026-0951757 / 2019-762-000867, 1 p., Mar 26, 2026. Rådgivningsnotat fra DCA - Nationalt Center for Fødevarer og Jordbrug.

Kragh, M , Dyrberg, H , Kristiansen, ST , Speed, M , Pedersen, P & Martiny, K 2026, 'Efficacy of a Transdiagnostic Sleep and Circadian Intervention for Outpatients With Sleep Problems and Depression, Attention Deficit Disorder, or Bipolar Disorder: A Randomised Controlled Trial', Journal of Sleep Research, vol. 35, no. 1, e70088. https://doi.org/10.1111/jsr.70088

Sobouti, F, Aryana, M, Mohammad-Rahimi, H, Dadgar, S, Alizadeh-Navaei, R & Rakhshan, V 2026, 'Efficacy of Automatic 3D Segmentation of the Upper Airway in CBCT or CT Scans via Artificial Intelligence Versus Manual Segmentation by Human Experts: A Systematic Review and Meta-Analysis', Clinical and Experimental Dental Research, vol. 12, no. 2, e70314. https://doi.org/10.1002/cre2.70314

Ellebaek, E, Weichenthal, M, Gavrilova, I, Asher, N, Terheyden, P, Utikal, J, Suljeviić, AJ, Pföhler, C, Stojkovski, I, Herbst, RA, Ugurel, S, Ruhlmann, C, Berrocal, A, Majem, M, Dujovic, B, Šabarić, V, Duvancic, T, Soria, A, Donia, M, Schmidt, H , Friis, RB, Gerasimov, A, Shalamanova-Deleva, G, Kontilev, A, Lev-Ari, S, Shapira, R, Piccin, L, Minisini, A, Özdemir, BC, Teterycz, P, Mariuk-Jarema, A, Ziogas, D, Anastasopoulou, A, Stefanou, D, Gutiérrez, L, Gebhardt, C, Dummer, R, Hauschild, A, Mohr, P, von Wasielewski, I, Meier, F, Haanen, J, Svane, IM, Ascierto, PA, Mangana, J, Rutkowski, P, Gogas, H, Bastholt, L & Schadendorf, D 2026, 'Efficacy of combined BRAF-MEK inhibitor second-line therapy in patients with non-resectable or metastatic BRAFV600-positive melanoma after prior immunotherapy: A retrospective EUMelaReg multicenter study', The European Journal of Cancer - Skin Cancer, vol. 4, 100773. https://doi.org/10.1016/j.ejcskn.2025.100773

Larsen, SØ, Axelgaard, S , Jønsson, IM, Brodersen, B, Kristensen, SB, Nielsen, BR, Hagstrøm, S & Borch, L 2026, 'Efficacy of low volume transanal irrigation in children with retentive fecal incontinence: A randomized controlled trial', Journal of Pediatric Gastroenterology and Nutrition, vol. 82, no. 2, pp. 425-433. https://doi.org/10.1002/jpn3.70279

Paul, A , Bleses, D & Rosholm, M 2026, 'Efficient targeting in childhood interventions', Journal of Human Resources, vol. 61, no. 1, pp. 161-184. https://doi.org/10.3368/jhr.0320-10756R4

Brøndal, HG & Lauritzen Dias, MN 2026, 'Efterord', Passepartout, pp. 153-154. <https://tidsskrift.dk/passepartout/article/view/165197>

Andersen, JE 2026, Efterskrift. in Friedrich Hölderlin, Hyperion. Forlaget Virkelig.

Iturbe-Espinoza, P , Elsgaard, L , Sapkota, R , Ellegaard-Jensen, L & Winding, A 2026, 'Eight-year effect of biochar amendment on soil properties, extracellular enzyme activity, N-cycling genes and microbiome structure in two Danish fallow soils', Microbiological Research, vol. 303, 128398. https://doi.org/10.1016/j.micres.2025.128398

Wray, KB 2026, Einflüsse auf Kuhn und das Verhältnis von Wissenschaftsgeschichte und Wissenshaftsphilosophie. in M Seidel (ed.), Thomas Kuhns Die Struktur wissenschaftlicher Revolutionen. De Gruyter, Klassiker Auslegen, pp. 15-28. https://doi.org/10.1515/9783111243924-003

Yates, MF, Dam Christensen, H & Larsen, AH 2026, 'Eksperter bag ny undersøgelse: Reformen har påvirket museernes forskning - men ikke på en entydig måde', Kulturmonitor. <https://kulturmonitor.dk/nyheder/debat/article18935729.ece>

Phan, NQT , Mamakhel, AH , Borup, AB , Bertelsen, AD , Kløve, MN & Iversen, BB 2026, 'Electrical Junction at the Substrate–Fe₃O₄Nanoparticle Interface Governs Oxygen Evolution Reaction Activity', ACS Applied Materials and Interfaces, vol. 18, no. 9, pp. 13872-13879. https://doi.org/10.1021/acsami.6c00310

Displaying results 1101 to 1150 out of 389656

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen