Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Nilsson, F, Davidsson, Å, Falås, P, Bengtsson, S, Bester, K & Jönsson, K 2019, 'Impact of activated sludge ozonation on filamentous bacteria viability and possible added benefits', Environmental Technology (United Kingdom), vol. 40, no. 20, pp. 2601-2607. https://doi.org/10.1080/09593330.2018.1447023

Lindhard, SM, Hamzeh, F, Gonzalez, VA, Wandahl, S & Ussing, LF 2019, 'Impact of Activity Sequencing on Reducing Variability', Journal of Construction Engineering and Management, vol. 145, no. 3, 04019001. https://doi.org/10.1061/(ASCE)CO.1943-7862.0001618

Carlsen, AH, Eriksen, JG, Godballe, C, Johansen, J, Sørensen, JA & Bjørndal, K 2019, 'Impact of age, comorbidity and WHO performance status on delay of treatment in patients undergoing fast-track work-up for head and neck cancer', Journal of Geriatric Oncology, vol. 10, no. 2, pp. 259-264. https://doi.org/10.1016/j.jgo.2018.06.003

Donatienne, A, Kristensen, HL, Canali, S, De Neve, S, Huyghebart, B & Stilmant, B 2019, Impact of agro-ecological service crops and their termination modes on soil mineral nitrogen dynamic at spring crop implantation. in A Messéa, D Drexler, I Heim, L Paresy, D Stilman & H Wille (eds), First European Conference on Crop Diversification: Book of Abstracts. INRA, pp. 148-149. https://doi.org/10.5281/zenodo.3516329

Donatienne, A, Kristensen, HL, Canali, S, De Neve, S, Sans Serra, FX, Huyghebart, B & Stilmant, B 2019, Impact of agro-ecological service crops and their termination strategies on soil mineral nitrogen availability, soil humidity, weeds development and cabbage harvest. in E Lorencowicz, J Uziak & B Huyghebaert (eds), Farm machinery and processes management in sustainable agriculture: X International Scientific Symposium FMPMSA 2019. Instytut Naukowo-Wydawniczy, Poland, pp. 395-400. https://doi.org/10.24326/fmpmsa.2019.1

Zemo, KH , Panduro, TE & Termansen, M 2019, 'Impact of biogas plants on rural residential property values and implications for local acceptance', Energy Policy, vol. 129, pp. 1121-1131. https://doi.org/10.1016/j.enpol.2019.03.008

Gamst-Jensen, H, Frishknecht Christensen, E, Lippert, F, Folke, F, Egerod, I, Brabrand, M, Tolstrup, JS, Thygesen, LC & Huibers, L 2019, 'Impact of caller's degree-of-worry on triage response in out-of-hours telephone consultations: A randomized controlled trial', Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine, vol. 27, no. 1, 44. https://doi.org/10.1186/s13049-019-0618-2

Hermann, L , Wenzel, A , Schropp, L & Matzen, LH 2019, 'Impact of CBCT on treatment decision related to surgical removal of impacted maxillary third molars: does CBCT change the surgical approach?', Dento maxillo facial radiology, vol. 48, no. 8, 20190209. https://doi.org/10.1259/dmfr.20190209

Öztürk, B , Johnsen, SP, Rock, ND, Pedersen, L & Pedersen, AB 2019, 'Impact of comorbidity on the association between surgery delay and mortality in hip fracture patients: a Danish nationwide cohort study', Injury, vol. 50, no. 2, pp. 424-431. https://doi.org/10.1016/j.injury.2018.12.032

Ørum, M , Jensen, K , Gregersen, M , Meldgaard, P & Damsgaard, EMS 2019, 'Impact of comprehensive geriatric assessment on short-term mortality in older patients with cancer: a follow-up study', European Journal of Cancer, vol. 116, pp. 27-34. https://doi.org/10.1016/j.ejca.2019.05.003

Kirkegård, J, Gaber, C, Lund, JL, Peacock-Hinton, S, Ladekarl, M , Heide-Jørgensen, U , Cronin Fenton, D & Mortensen, FV 2019, 'Impact of Concurrent Acute Pancreatitis on Pancreatic Cancer Stage, Treatment, and Prognosis: A Study of Two Cohorts', 13th Congress of the European – African Hepato-Pancreato-Biliary Association, Amsterdam, Netherlands, 03/06/2019 - 05/06/2019.

Holst, LM, Kronborg, JB, Idorn, L, Bjerre, JV, Vejlstrup, N, Juul, K & Ravn, HB 2019, 'Impact of congenital heart surgery on quality of life in children and adolescents with surgically corrected Ventricular Septal Defect, Tetralogy of Fallot, and Transposition of the Great Arteries', Cardiology in the Young, vol. 29, no. 8, pp. 1082-1087. https://doi.org/10.1017/S1047951119001604

Zhu, K , Victoria, M , Brown, T , Andresen, GB & Greiner, M 2019, 'Impact of CO₂ prices on the design of a highly decarbonised coupled electricity and heating system in Europe', Applied Energy, vol. 236, pp. 622-634. https://doi.org/10.1016/j.apenergy.2018.12.016

Pedersen, J, Thorsen, SV, Andersen, MF, Hanvold, TN, Schlünssen, V & Bültmann, U 2019, 'Impact of depressive symptoms on worklife expectancy: A longitudinal study on Danish employees', Occupational & Environmental Medicine, vol. 76, no. 11, pp. 838-844. https://doi.org/10.1136/oemed-2019-105961

Ellert, J, Christiansen, EH , Maeng, M, Raungaard, B, Jensen, SE, Kristensen, SD, Veien, KT, Junker, AB, Jakobsen, L, Aarøe, J, Terkelsen, CJ , Kahlert, J, Villadsen, AB, Bøtker, HE & Jensen, LO 2019, 'Impact of diabetes on clinical outcomes after revascularization with sirolimus-eluting and biolimus-eluting stents with biodegradable polymer from the SORT OUT VII trial', Catheterization and Cardiovascular Interventions, vol. 93, no. 4, pp. 567-573. https://doi.org/10.1002/ccd.27891

Paolo, D, Bianchi, G, Morelli, CF, Speranza, G, Campanelli, G, Kidmose, U & Lo Scalzo, R 2019, 'Impact of drying techniques, seasonal variation and organic growing on flavor compounds profiles in two Italian tomato varieties', Food Chemistry, vol. 298, 125062. https://doi.org/10.1016/j.foodchem.2019.125062

Naghizadeh, M , Dalgaard, TS & Engberg, RM 2019, 'Impact of encapsulated butyrate upon intestinal microbiota, pH and digestive function of broilers suffering from dysbacteriosis', 22 European Symposium on Poultry Nutrition, Gdansk, Poland, 10/06/2019 - 13/06/2019.

Wu, S, Wu, H, Button, M, Konnerup, D & Brix, H 2019, 'Impact of engineered nanoparticles on microbial transformations of carbon, nitrogen, and phosphorus in wastewater treatment processes – A review', Science of the Total Environment, vol. 660, pp. 1144-1154. https://doi.org/10.1016/j.scitotenv.2019.01.106

Bojsen, TB & Tambo, T 2019, 'Impact of enterprise architecture-based complexity assessment on ppm: Multitrack segmentation', Paper presented at 42nd Information systems research seminar in Scandinavia (IRIS), Nokia, Finland, 11/08/2019 - 14/08/2019 pp. 1-12.

Lyngsø, J, Kesmodel, US, Bay, B , Ingerslev, HJ, Nybo Andersen, AM & Ramlau-Hansen, CH 2019, 'Impact of female daily coffee consumption on successful fertility treatment: a Danish cohort study', Fertility and Sterility, vol. 112, no. 1, pp. 120-129.e2. https://doi.org/10.1016/j.fertnstert.2019.03.014

Zintchouk, D , Gregersen, M , Lauritzen, T & Damsgaard, EM 2019, 'Impact of Geriatrician-Performed Comprehensive Geriatric Care on Medication Use and Cognitive Function in Older Adults Referred to a Non-Hospital-Based Rehabilitation Unit', American Journal of Medicine, vol. 132, no. 1, pp. 93-102. https://doi.org/10.1016/j.amjmed.2018.09.030

Varnum, C, Pedersen, AB, Rolfson, O, Rogmark, C, Furnes, O, Hallan, G, Mäkelä, K, de Steiger, R, Porter, M & Overgaard, S 2019, 'Impact of hip arthroplasty registers on orthopaedic practice and perspectives for the future', EFORT open reviews, vol. 4, no. 6, pp. 368-376. https://doi.org/10.1302/2058-5241.4.180091

Kristiansen, SB , Pælestik, KB , Johnsen, J , Jespersen, NR , Pryds, K , Hjortbak, MV , Vibjerg Jensen, R & Bøtker, HE 2019, 'Impact of hyperglycemia on myocardial ischemia-reperfusion susceptibility and ischemic preconditioning in hearts from rats with type 2 diabetes', Cardiovascular Diabetology, vol. 18, no. 1, 66. https://doi.org/10.1186/s12933-019-0872-7

Poulsen, AH, Raaschou-Nielsen, O, Peña, A, Hahmann, AN, Nordsborg, RB, Ketzel, M , Brandt, J & Sørensen, M 2019, 'Impact of Long-Term Exposure to Wind Turbine Noise on Redemption of Sleep Medication and Antidepressants: A Nationwide Cohort Study', Environmental Health Perspectives, vol. 127, no. 3, 037005. https://doi.org/10.1289/EHP3909

Ntsoane, ML, Luca, A, Zude-Sasse, M, Sivakumar, D & Mahajan, PV 2019, 'Impact of low oxygen storage on quality attributes including pigments and volatile compounds in ‘Shelly’ mango', Scientia Horticulturae, vol. 250, pp. 174-183. https://doi.org/10.1016/j.scienta.2019.02.041

Pérez, B, Nykvist, H, Brøgger, AF, Larsen, MB & Falkeborg, MF 2019, 'Impact of macronutrients printability and 3D-printer parameters on 3D-food printing: A review', Food Chemistry, vol. 287, pp. 249-257. https://doi.org/10.1016/j.foodchem.2019.02.090

Dold, C, Hatfield, JL, Prueger, JH, Sauer, TJ, Moorman, TB & Wacha, KM 2019, 'Impact of Management Practices on Carbon and Water Fluxes in Corn–Soybean Rotations', Agrosystems, Geosciences and Environment, vol. 2, no. 1, pp. 1-8. https://doi.org/10.2134/age2018.08.0032

Andriollo, T, Zhang, Y, Fæster, S, Thorborg, J & Hattel, JH 2019, 'Impact of micro-scale residual stress on in-situ tensile testing of ductile cast iron: Digital volume correlation vs. model with fully resolved microstructure vs. periodic unit cell', Journal of the Mechanics and Physics of Solids, vol. 125, pp. 714-735. https://doi.org/10.1016/j.jmps.2019.01.021

Spin-Neto, R , Kruse, C , Kirkevang, L-L , Poulsen, LH & Wenzel, A 2019, 'Impact of motion artefact correction on diagnostic accuracy of CBCT for apical periodontitis assessment: an ex vivo pilot study', 22ND International Congress of DENTO-MAXILLO-FACIAL RADIOLOGY , Philadelphia, United States, 22/08/2019 - 25/08/2019.

Machado Santaella, G, Wenzel, A, Haiter-Neto, F, Rosalen, PL & Spin-Neto, R 2019, 'Impact of movement and motion-artefact correction on image quality and interpretability in CBCT units with aligned and lateral-offset detectors', 22ND International Congress of DENTO-MAXILLO-FACIAL RADIOLOGY , Philadelphia, United States, 22/08/2019 - 25/08/2019.

Castellano, D, Eyre-Walker, A & Munch, K 2019, 'Impact of mutation rate and selection at linked sites on DNA variation across the genomes of humans and other homininae', Genome Biology and Evolution, vol. 12, no. 1, pp. 3550-3561. https://doi.org/10.1093/gbe/evz215

Glassou, EN , Kristensen, N , Møller, BK , Erikstrup, C , Hansen, TB & Pedersen, AB 2019, 'Impact of preadmission anti-inflammatory drug use on the risk of RBC transfusion in elderly hip fracture patients: a Danish nationwide cohort study, 2005-2016', Transfusion, vol. 59, no. 3, pp. 935-944. https://doi.org/10.1111/trf.15110

Røge, R, Nielsen, S, Riber-Hansen, R & Vyberg, M 2019, 'Impact of Primary Antibody Clone, Format, and Stainer Platform on Ki67 Proliferation Indices in Breast Carcinomas', Applied immunohistochemistry & molecular morphology : AIMM, vol. 27, no. 10, pp. 732-739. https://doi.org/10.1097/PAI.0000000000000799

Ozaki, Y, Garcia-Garcia, HM, Hideo-Kajita, A, Kuku, KO, Haude, M, Ince, H, Abizaid, A, Tölg, R, Lemos, PA, von Birgelen, C, Christiansen, EH, Wijns, W, Escaned, J, Dijkstra, J & Waksman, R 2019, 'Impact of procedural characteristics on coronary vessel wall healing following implantation of second-generation drug-eluting absorbable metal scaffold in patients with de novo coronary artery lesions: an optical coherence tomography analysis', European Heart Journal Cardiovascular Imaging, vol. 20, no. 8, pp. 916-924. https://doi.org/10.1093/ehjci/jey210

Su, J, Hughes, AD, Simonsen, U , Nielsen-Kudsk, JE, Parker, KH, Howard, LS & Mellemkjaer, S 2019, 'Impact of Pulmonary Endarterectomy on Pulmonary Arterial Wave Propagation and Reservoir Function', American Journal of Physiology: Heart and Circulatory Physiology, vol. 317, no. 3, pp. H505-H516. https://doi.org/10.1152/ajpheart.00181.2019

Rasmussen, NF, Rubin, KH, Stougaard, M, Tjonneland, A, Stenager, E, Hetland, ML, Glintborg, B, Bygum, A & Andersen, V 2019, 'Impact of red meat, processed meat and fibre intake on risk of late-onset chronic inflammatory diseases: prospective cohort study on lifestyle factors using the Danish 'Diet, Cancer and Health' cohort (PROCID-DCH): protocol', BMJ Open, vol. 9, no. 3, 024555. https://doi.org/10.1136/bmjopen-2018-024555

Danso, EO, Yakubu, A, Kugblenu-Darrah, Y, Arthur, E , Manevski, K, Sabi, EB, Abenney-Mickson, S, Ofori, K, Plauborg, F & Andersen, MN 2019, 'Impact of rice straw biochar and irrigation on maize yield, intercepted radiation and water productivity in a tropical sandy clay loam', Field Crops Research, vol. 243, 107628. https://doi.org/10.1016/j.fcr.2019.107628

Sembach, FE, Fink, LN, Johansen, T, Boland, BB, Secher, T, Thrane, ST, Nielsen, JC, Fosgerau, K, Vrang, N, Jelsing, J, Pedersen, TX & Ostergaard, MV 2019, 'Impact of sex on diabetic nephropathy and the renal transcriptome in UNx db/db C57BLKS mice', Physiological Reports, vol. 7, no. 24, 14333. https://doi.org/10.14814/phy2.14333

Ikuta, M, Iida, T, Kothari, M , Shimada, A, Komiyama, O & Svensson, P 2019, 'Impact of sleep bruxism on training-induced cortical plasticity', Journal of Prosthodontic Research, vol. 63, no. 3, pp. 277-282. https://doi.org/10.1016/j.jpor.2018.12.008

Leite, FRM , Nascimento, GG , Baake, S, Pedersen, LD, Scheutz, F & López, R 2019, 'Impact of smoking cessation on periodontitis: A systematic review and meta-analysis of prospective longitudinal observational and interventional studies', Nicotine & Tobacco Research, vol. 21, no. 12, pp. 1600-1608. https://doi.org/10.1093/ntr/nty147

Hermanowski, P & Piotrowski, JA 2019, 'Impact of subglacial groundwater flow on the formation of Stargard drumlin field, NW Poland – insights from numerical modelling', EGU 2019, Vienna, Austria, 08/04/2019 - 12/04/2019.

Schelde, AB , Schmidt, M , Madsen, M, Nielsen, SS, Frøkiær, J & Christiansen, CF 2019, 'Impact of the Charlson Comorbidity Index score on risk prediction by single-photon emission computed tomography myocardial perfusion imaging following myocardial infarction', Clinical epidemiology, vol. 11, pp. 901-910. https://doi.org/10.2147/CLEP.S211555

Buitenhuis, B , Lassen, J , Noel, SJ, Plichta, DR, Sørensen, P , Difford, GF & Poulsen, NA 2019, 'Impact of the rumen microbiome on milk fatty acid composition of Holstein cattle', Genetics Selection Evolution, vol. 51, no. 1, 23. https://doi.org/10.1186/s12711-019-0464-8

Daraghmeh, OA, Petersen, CT, Munkholm, LJ, Znova, L, Obour, PB, Nielsen, SK & Green, O 2019, 'Impact of tillage intensity on clay loam soil structure', Soil Use and Management, vol. 35, no. 3, pp. 388-399. https://doi.org/10.1111/sum.12501

Claassen, YHM, Hartgrink, HH, de Steur, WO, Dikken, JL, van Sandick, JW, van Grieken, NCT, Cats, A, Trip, AK, Jansen, EPM, Kranenbarg, WMM-K, Braak, JPBM, Putter, H, van Berge Henegouwen, MI, Verheij, M & van de Velde, CJH 2019, 'Impact of upfront randomization for postoperative treatment on quality of surgery in the CRITICS gastric cancer trial', Gastric Cancer, vol. 22, no. 2, pp. 369-376. https://doi.org/10.1007/s10120-018-0875-1

Deichmann, MM , Andersen, MN , Thomsen, IK & Børgesen, CD 2019, 'Impacts of controlled drainage during winter on the physiology and yield of winter wheat in Denmark', Agricultural Water Management, vol. 216, pp. 118-126. https://doi.org/10.1016/j.agwat.2019.01.013

Denton, PW, Søgaard, OS & Tolstrup, M 2019, 'Impacts of HIV cure interventions on viral reservoirs in tissues', Frontiers in Microbiology, vol. 10, no. AUG, 1956. https://doi.org/10.3389/fmicb.2019.01956

Yang, X, Song, C, Zhu, C, Liang, C & Sun, R 2019, 'Impacts of Misalignments on Mesh Behaviors of Face-Hobbed Hypoid Gear Considering System Deformation', IEEE Access, vol. 7, pp. 79244 - 79253. https://doi.org/10.1109/ACCESS.2019.2923532

Xu, B, Tian, H, Sabel, CE & Xu, B 2019, 'Impacts of Road Traffic Network and Socioeconomic Factors on the Diffusion of 2009 Pandemic Influenza A (H1N1) in Mainland China', International Journal of Environmental Research and Public Health, vol. 16, no. 7, 1223. https://doi.org/10.3390/ijerph16071223

Asschenfeldt, B , Evald, L , Eskildsen, SF , Heiberg, J , Østergaard, L & Hjortdal, VE 2019, 'Impaired executive function in adults with a repaired atrial septal defect', Circulation, vol. 140, no. Suppl 1, A13312. <https://www.ahajournals.org/doi/abs/10.1161/circ.140.suppl_1.13312>

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