Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Hovgaard, MK, Hansen, JB , Skafte, A , Olsen, P & Brincker, R 2015, Study of vibration based SHM technologies, Part III: Localization using statistical learning theory. in Structural Health Monitoring 2015 - System Reliability for Verification and Implementation: Proceedings of the 10th International Workshop on Structural Health Monitoring. DEstech Publications, International Workshop on Structural Health Monitoring, California, United States, 01/09/2015. https://doi.org/10.12783/SHM2015/305

Hansen, JB, Hovgaard, MK, Olsen, P , Skafte, A & Brincker, R 2015, Study of Vibration Based SHM Technologies, Part IV: Localization Using Physical-based Methods. in Structural Health Monitoring 2015 - Sýstem Reliability for Verification and Implementation: Proceedings of the 10th International Workshop on Structural Health Monitoring. DEstech Publications, International Workshop on Structural Health Monitoring, California, United States, 01/09/2015. https://doi.org/10.12783/SHM2015/171

Olsen, P , Hovgaard, MK , Hansen, JB , Skafte, A & Brincker, R 2015, Study of vibration based technologies, Part I: Experimental setup and Automated Identification. in Structural Health Monitoring 2015 - System Reliability for Verification and Implementation: Proceedings of the 10th International Workshop on Structural Health Monitoring. DEstech Publications, International Workshop on Structural Health Monitoring, California, United States, 01/09/2015. https://doi.org/10.12783/SHM2015/184

Hovgaard, MK, Hansen, JB , Skafte, A , Olsen, P & Brincker, R 2015, Study of vibration based technologies, Part II: Detection. in Structural Health Monitoring 2015 - Sýstem Reliability for Verification and Implementation: Proceedings of the 10th International Workshop on Structural Health Montoring . DEstech Publications, International Workshop on Structural Health Monitoring, California, United States, 01/09/2015. https://doi.org/10.12783/SHM2015/183

Hogg, D, Andersen, MS, Elliott, T, Sherrington, C, Vergunst, T, Ettlinger, S, Elliott, L, Hudson, J, Ten Brinck, P & Withana, S 2015, Study on Environmental Fiscal Reform Potential in 14 EU Member States: Main Report & Appendices: Final Report to DG Environment of the European Commission. Bruxelles. <http://ec.europa.eu/environment/integration/green_semester/studies_en.htm>

Schmidt, U 2015, 'Styring av religion: Tros- og livssynspolitiske tendenser etter "Det livssynsåpne samfunn"', Teologisk Tidsskrift, vol. 4, no. 3, pp. 218-237.

Andersen, SC & Loftager, J 2015, Styringseffektivitet og demokrati. in Politologisk årbog 2014-2015. København, pp. 82-85.

Whitta-Jacobsen, HJ, Amundsen, ES, Svarer, M & Dalgaard, C-J 2015, 'Styrk den globale kamp for klimaet', Boersen.

Klose-Jensen, R , Hartlev, LB , Boel, LWT , Laursen, MB, Steengaard-Pedersen, K, Keller, KK & Hauge, EM 2015, 'Subchondral Bone Turnover, but not Bone Volume, is Increased in Early Stage Osteoarthritic Lesions in the Human Hip Joint', Osteoarthritis and Cartilage.

Chen, R, Zhang, L, Ge, C, Tseng, M, Bai, R, Qu, Y, Beer, C , Autrup, H & Chen, C 2015, 'Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation', Chemical Research in Toxicology, vol. 28, no. 3, pp. 440-50. https://doi.org/10.1021/tx5004003

Christiansen, MK , Jensen, JM , Bøtker, HE & Jensen, HK 2015, 'Subclinical Coronary Plaque Burden in Asymptomatic Relatives of Patients With Documented Premature Coronary Artery Disease', American Heart Association Scientific Sessions, Orlando, United States, 07/11/2015 - 11/11/2015.

Funder, JA, Christiansen, R, Sandahl, OG, Hjortdal, VE , Nygaard, H & Hasenkam, JM 2015, 'Subcoronary Stentless Aortic Valves are not Superior to Supra-Annular Stented Valves Regarding Turbulent Stress', The Journal of Heart Valve Disease, vol. 24, pp. 712-718.

Alluri, V, Toiviainen, P & Brattico, E 2015, 'Subcortical network hub differences in musicians and non-musicians during continuous listening to music', ICME4, Geneve, Switzerland, 12/10/2015 - 16/10/2015.

Chen, L 2015, 'Sub-Gaussian heat kernel estimates and quasi Riesz transforms for 1 ≤ p ≤ 2', Publicacions Matematiques, vol. 59, no. 2, pp. 313-338. https://doi.org/10.5565/PUBLMAT_59215_03

Meyer, R, Engelsgaard, PK, Sonnenborg, TO, Piotrowski, JA, Hinsby, K, Jørgensen, F & Høyer, A-S 2015, 'Subglacial groundwater flow pattern and its influence on salt water intrusions - a numerical model approach from southern Denmark', G@GPS Project International Workshop, Tallinn, Estonia, 05/07/2015 - 09/07/2015.

Pedersen, AB , Nielsen, HØ , Zandersen, M , Termansen, M & Oddershede, JS 2015, Subgroup: Agriculture. Case-study: Climate adaptation in two Danish rural municipalities. <http://base-adaptation.eu/sites/default/files/case_studies/02_Holstebro_Lolland_CSLD.pdf>

Sørensen, AC 2015, 'Subjective definition of traits and economic values for selction of organic sows in Denmark', 66th Annual Meeting of the European Federation of Animal Science, Warsaw, Poland, 31/08/2015 - 04/09/2015. https://doi.org/10.3920/978-90-8686-816-2

Sørensen, AC 2015, Subjective definition of traits and economic values for selction of organic sows in Denmark. in Book of Abstract of the 66th Annual Meeting of the European Federation of Animal Science. vol. 21, Wageningen Academic Publishers, The Netherlands, EAAP Book of Abstracts, pp. 465-465, 66th Annual Meeting of the European Federation of Animal Science, Warsaw, Poland, 31/08/2015. https://doi.org/10.3920/978-90-8686-816-2

Christensen, JW, Andersen, RM, Hawson, L, von Borstel, UK, Roepstorff, L & Egenvall, A 2015, Subjective scoring of rideability by professional riders - is it linked to rein tension and occurrence of conflict behavior? in C Heleski & K Merkies (eds), 11th International Conference International Society for Equitation Science. pp. 28-28, 11th International Equitation Science Conference, Vancouver, Denmark, 05/08/2015.

Olesen, MH , Thomsen, DK & OToole, MS 2015, 'Subjective well-being: Above neuroticism and extraversion, autonomy motivation matters', Personality and Individual Differences, vol. 77, pp. 45-49. https://doi.org/10.1016/j.paid.2014.12.033

Maslo, E 2015, Subjektive mål som drivkraft for sprog og læring. in B Kristjánsdóttir, E Maslo, LK Svarstad, L Rohde & B Dahlgreen (eds), Læringsrum. Aarhus Universitetsforlag, Aarhus, Sprogforum, no. 61, pp. 64-72.

Kerzel, M 2015, Subjektiv skattepligt. in J Pedersen, M Kerzel, J Ferniss & CH Eriksen (eds), Skatteretten 1. 7 edn, Karnov Group, Kbh., pp. 716-774.

Pedersen, J 2015, Subjektiv skattepligt. in J Pedersen, CH Eriksen, M Kerzel & J Ferniss (eds), Skatteretten 2. 7 edn, Karnov Group, Kbh.

Laursen, AN 2015, Subjektiv skattepligt for fysiske personer. in A Michelsen, S Askholt, J Bolander, J Engsig, IL Jeppesen, AN Laursen & L Madsen (eds), Lærebog om indkomstskat. 16 edn, Djøf Forlag, Kbh., pp. 753-810.

Madsen, L 2015, Subjektiv skattepligt for selskaber. in A Michelsen, S Askholt, J Bolander, J Engsig, IL Jeppesen, AN Laursen & L Madsen (eds), Lærebog om indkomstskat. 16 edn, Djøf Forlag, Kbh., pp. 811-856.

Pakyari, H & Enkegaard, A 2015, 'Sublethal and Transgenerational Effects of Abamectin on the Biological Performance of the Predatory Thrips Scolothrips longicornis (Thysanoptera: Thripidae)', Journal of Economic Entomology, vol. 108, no. 2, pp. 559-565. https://doi.org/10.1093/jee/tou098

Pakyari, H & Enkegaard, A 2015, 'Sublethal effects of abamectin and fenpropathrin on the consumption of Tetranychus urticae eggs by Scolothrips longicornis', Systematic and Applied Acarology, vol. 20, no. 4, pp. 357-365. https://doi.org/10.11158/saa.20.4.1

Pinder, C, Vermeulen, J, Beale, R & Hendley, R 2015, 'Subliminal Priming of Nonconscious Goals on Smartphones', MobileHCI '15 Adjunct, pp. 825-830. https://doi.org/10.1145/2786567.2793707

Bury, M & Schwiegelshohn, C 2015, 'Sublinear estimation of weighted matchings in dynamic data streams', Lecture Notes in Computer Science, pp. 263-274. https://doi.org/10.1007/978-3-662-48350-3_23

Tolstrup, J 2015, Subnational Level: Russian Support for Secessionism and Pockets of Autocracy. in A Obydenkova & A Libman (eds), Autocratic and Democratic External Influences in Post-Soviet Eurasia. Ashgate, pp. 109-132.

Etzold, F, Howard, IA, Forler, N, Melnyk, A, Andrienko, D, Hansen, MR & Laquai, F 2015, 'Sub-ns triplet state formation by non-geminate recombination in PSBTBT:PC70BM and PCPDTBT:PC60BM organic solar cells', Energy & Environmental Science, vol. 8, no. 5, pp. 1511-1522. https://doi.org/10.1039/c4ee03630a

Jensen, K, Schal, C & Silverman, J 2015, 'Suboptimal nutrient balancing despite dietary choice in glucose-averse German cockroaches, Blattella germanica', Journal of Insect Physiology, vol. 81, pp. 42-47. https://doi.org/10.1016/j.jinsphys.2015.07.001

Bondeven, P , Laurberg, S, Hagemann-Madsen, RH & Ginnerup, B 2015, 'Suboptimal surgery and omission of neoadjuvant therapy for upper rectal cancer is associated with a high risk of local recurrence', Colorectal Disease, vol. 17, no. 3, pp. 216-224. https://doi.org/10.1111/codi.12869

Simeone, AL, Velloso, E & Gellersen, H 2015, Substitutional reality: using the physical environment to design virtual reality experiences. in Proceedings of the 33rd Annual ACM Conference on Human Factors in Computing Systems. ACM, pp. 3307-3316. https://doi.org/10.1145/2702123.2702389

Lessmeister, A, Schumann, K, Lykke, AM, Heubach, K, Thiombiano, A & Hahn, K 2015, 'Substitution of the most important and declining wild food species in South-East Burkina Faso.', Flora et Vegetatio Sudano-Sambesica, vol. 18, pp. 11-20.

Dugan, HA, Doran, PT, Tulaczyk, S, Mikucki, JA, Arcone, SA, Auken, E , Schamper, C & Virginia, RA 2015, 'Subsurface imaging reveals a confined aquifer beneath an ice-sealed Antarctic lake', Geophysical Research Letters (Online), vol. 42, no. 1, pp. 96-103. https://doi.org/10.1002/2014GL062431

Zeinali, B , Madsen, JK, Raghavan, P & Moradi, F 2015, Sub-Threshold SRAM Design in 14Nm FinFET Technology with Improved Access Time and Leakage Power. in Proceedings of the IEEE Computer Society Annual Symposium on VLSI (ISVLSI). vol. 2015, IEEE, Proceedings of the IEEE Computer Society Annual Symposium on VLSI, vol. 2015, pp. 74-79, ISVLSI, Montpellier, France, 08/07/2015. https://doi.org/10.1109/ISVLSI.2015.73

Bregnhøj, M , Pimenta, FM, Poronik, YM, Gryko, DT & Ogilby, PR 2015, 'Subtle structural changes in octupolar merocyanine dyes influence the photosensitized production of singlet oxygen.', Photochemical & Photobiological Sciences, vol. 14, no. 6, pp. 1138-1146. https://doi.org/10.1039/C5PP00080G

Leenders, M, Siersema, PD, Overvad, K, Tjønneland, A, Olsen, A, Boutron-Ruault, M-C, Bastide, N, Fagherazzi, G, Katzke, V, Kühn, T, Boeing, H, Aleksandrova, K, Trichopoulou, A, Lagiou, P, Klinaki, E, Masala, G, Grioni, S, De Magistris, MS, Tumino, R, Ricceri, F, Peeters, PHM, Lund, E, Skeie, G, Weiderpass, E, Quirós, JR, Agudo, A, Sánchez, M-J, Dorronsoro, M, Navarro, C, Ardanaz, E, Ohlsson, B, Jirström, K, Van Guelpen, B, Wennberg, M, Khaw, K-T, Wareham, N, Key, TJ, Romieu, I, Huybrechts, I, Cross, AJ, Murphy, N, Riboli, E & Bueno-de-Mesquita, HB 2015, 'Subtypes of fruit and vegetables, variety in consumption and risk of colon and rectal cancer in the European Prospective Investigation into Cancer and Nutrition', International Journal of Cancer, vol. 137, no. 11, pp. 2705-2714. https://doi.org/10.1002/ijc.29640

Horst, M & Irwin, A 2015, Succeeding in a European Research Environment: Eleven Lessons from Denmark. in R Dingwall & MB McDonnell (eds), The SAGE Handbook of Research Management. SAGE Publications, pp. 247-258.

Nygaard, S & Bønding, S 2015, Succesfuldt studentersymposium om vikingetid og middelalder. <http://studerende.au.dk/studier/fagportaler/arts/aktuelt/nyheder/vis-nyhed/browse/6/artikel/succesfuldt-studentersymposium-om-vikingetid-og-middelalder/>

Lueg, R & Louisa, V 2015, 'Success factors in Balanced Scorecard implementations: A literature review', Management Revue, vol. 26, no. 4, pp. 306-327. https://doi.org/DOI 10.1688/mrev-2015-04-Lueg

Müller, SD & Skau, SA 2015, 'Success factors influencing implementation of e-government at different stages of maturity: A literature review', International Journal of Electronic Governance, vol. 7, no. 2, pp. 136-170. https://doi.org/10.1504/IJEG.2015.069495

Parz-Gollner, R, Marion, L, Paquet, J-Y, Volponi, S, Bregnballe, T & Pihl, S 2015, 'Successful census of wintering Cormorants Phalacrocorax carbo in Europe in January 2013', Wetlands International Cormorant Research Group Bulletin, vol. 8, pp. 7-8.

Pulczynski, EJ, Kuittinen, O, Erlanson, M, Hagberg, H, Fosså, A, Eriksson, M, Nordstrøm, M, Østenstad, B, Fluge, Ø, Leppä, S, Fiirgaard, B, Bersvendsen, H & Fagerli, U-M 2015, 'Successful change of treatment strategy in elderly patients with primary central nervous system lymphoma by de-escalating induction and introducing temozolomide maintenance: results from a phase II study by the Nordic Lymphoma Group', Haematologica, vol. 100, no. 4, pp. 534-40. https://doi.org/10.3324/haematol.2014.108472

Rathleff, CR, Cavallius, C, Jensen, HP, Simonsen, OH, Rasmussen, S, Kaalund, S & Ostgaard, SE 2015, 'Successful conservative treatment of patients with MRI-verified meniscal lesions', Knee Surgery, Sports Traumatology, Arthroscopy, vol. 23, no. 1, pp. 178-183. https://doi.org/10.1007/s00167-013-2494-z

Høgsbro, M, Agger, A & Johansen, LV 2015, 'Successful loading of a bone-anchored hearing implant at two weeks after surgery: randomized trial of two surgical methods and detailed stability measurements', Otology & Neurotology, vol. 36, no. 2, pp. e51-7. https://doi.org/10.1097/MAO.0000000000000647

Breitenbauch, MTW & Tøttrup, A 2015, 'Successful Palliation of a Malignant Cologastric Fistula with a Covered Self-Expanding Metal Stent', Clinical endoscopy, vol. 48, no. 6, pp. 576-8. https://doi.org/10.5946/ce.2015.48.6.576

Beaudet, A, Breman, A, Normand, E, Qdaisat, S, Chow, J, U'Ren, L, Stilwell, J, Singh, R, Uldbjerg, N, Seubert, R, Kaldjian, E, Schelde, P & Kølvrå, S 2015, 'Successful perrformance of array CGH on fetal cells isolated from the maternal blood during the first trimester: Oral precentation', 19th International Conference of Prenatal Diagnosis and Therapy, Wasington DC, United States, 12/07/2015 - 15/07/2015.

Bayen, P, Lykke, AM & Thiombiano, A 2015, 'Success of three soil restoration techniques on seedling survival and growth of three plant species in the Sahel of Burkina Faso (West Africa)', Journal of Forestry Research, vol. 27, no. 2, pp. 313-320. https://doi.org/10.1007/s11676-015-0159-0

Displaying results 167801 to 167850 out of 389632

3357

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen