Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Brandenbarg, D, Maass, SWMC, Geerse, OP, Stegmann, ME, Handberg, C, Schroevers, MJ & Duijts, SFA 2019, 'A systematic review on the prevalence of symptoms of depression, anxiety and distress in long-term cancer survivors: Implications for primary care', European Journal of Cancer Care Online, vol. 28, no. 3, e13086. https://doi.org/10.1111/ecc.13086

Brunbjerg, AK, Bruun, HH, Brøndum, L, Classen, AT, Dalby, L, Fog, K, Frøslev, TG, Goldberg, I, Hansen, AJ, Hansen, MDD, Høye, TT, Illum, AA, Læssøe, T, Newman, G, Skipper, L, Søchting, U & Ejrnæs, R 2019, 'A systematic survey of regional multi-taxon biodiversity: evaluating strategies and coverage', BMC Ecology, vol. 19, 43. https://doi.org/10.1186/s12898-019-0260-x

Bjerge, K , Fynbo, HOU & Johansen, JG 2019, 'A system on programmable chip design of a digitizer with improved trapezoidal filter validation', Microprocessors and Microsystems, vol. 65, pp. 7-13. https://doi.org/10.1016/j.micpro.2018.12.004

Kristensen, OS 2019, At adskille for at inkludere - inklusion og eksklusion af anbragte børn og unge. in KE Petersen & JH Hansen (eds), Inklusion og eksklusion . Hans Reitzels Forlag, København, pp. 277-289.

Ravn, I 2019, At agere facilitator. in A Matthiessen, SF Gerholt, AM Møller & B Zeeberg (eds), Bedre møder på tværs: facilitering, processer, beslutninger. Samfundslitteratur, Frederiksberg, pp. 67-82.

Ngqulana, S, Christiansen, M, Hoelzel, AR, Galatius, A, Pistorius, P, Plon, S & Greg Hofmeyr, GJ 2019, 'A take on years of wrangling over a controversial taxonomy: Delphinus spp. in South African waters', Maritime Networks and Urbanism in the Early Medieval World, Roskilde, Denmark, 11/04/2013 - 12/04/2013.

Özbek, M , Bohn, A & Berntsen, D 2019, 'A Tale of Two Cultural Life Scripts: Immigrants’ Perception of a Normative Life', The 60th Annual Meeting of the Psychonomic Society, Montréal, Canada, 14/11/2019 - 17/11/2019.

Krejsler, JB 2019, At analysere med Gilles Deleuze: En rejse i nomadebegreber, tilblivelser og kontrolsamfund. Nyt fra Samfundsvidenskaberne, Frederiksberg.

Skaanes, T 2019, 'A tangible generator of time itself?', Paper presented at SPIRITools, København, Denmark, 02/05/2019 - 03/05/2019.

Thylstrup, B & Hesse, M 2019, At arbejde med problematisk brug af rusmidler er at arbejde med psykiske vanskeligheder. in B Bjerge & E Houborg (eds), Rusmiddelbrugere i krydsfeltet mellem sektorer og fagligheder. Aarhus Universitetsforlag, Aarhus, Samfund og Rusmidler, no. 6, pp. 183-205.

Prattichizzo, D, Malvezzi, M, Chinello, F & Pacchierotti, C, A task-custom finger device for kinesthetic and cutaneous feedback, Patent No. WO2017194527A1. <https://patentimages.storage.googleapis.com/43/cb/60/56ed9253bd1f16/WO2017194527A1.pdf>

Skyggebjerg, AK 2019, 'At blive til et træ: Døden. Prisbelønnede Anna Jacobina Jacobsen tegner og fortæller stilfærdigt om kroppens forfald og døden som en befrielse.', Weekendavisen, vol. Bøger, 12, pp. 6.

Musaeus, P 2019, 'At dømme ud fra porteføljen: Om undervisningsteknikker og teknologier', DUN Konferencen 2019, Odense, Denmark, 28/05/2019 - 29/05/2019 pp. 1.

Eggers-Kaas, T , Pedersen, JB , Hoggard, CS , Sauer, F, Hilgart, J & Riede, F 2019, 'A Technological and Typological Analysis of Lithic Material from Skovmosen I, Denmark', Danish Journal of Archaeology, vol. 8, pp. 1-18. https://doi.org/10.7146/dja.v8i0.112232

Jensen, SR , Kirkegaard, PH, Postiglione, G & Strange, A 2019, A tectonic approach to energy renovation of dwellings - the case of Gellerup. in PJS Cruz (ed.), Structures and Architecture: Bridging the Gap and Crossing Borders - Proceedings of the 4th International Conference on Structures and Architecture, ICSA 2019. Taylor & Francis, London, pp. 99-108, International Conference on Structures and Architecture (ICSA 2019), Lisbon, Portugal, 24/07/2019. https://doi.org/10.1201/9781315229126-12

Jensen, SR , Kirkegaard, PH, Postiglione, G & Strange, A 2019, A tectonic aproach to energy renovation of dwellings: the case of Gellerup. in P J. S. Cruz (ed.), Structures and architecture: bridging the gap and crossing borders. CRC Press, Leiden, pp. 99-108. <https://books.google.dk/books?id=mQSiDwAAQBAJ&pg=PA99&dq=%22A+tectonic+approach+to+energy+renovation+of+dwellings+-+The+case+of+Gellerup%22&hl=da&sa=X&ved=0ahUKEwiV5sToye7mAhVRLFAKHaE5CzgQ6AEIJzAA#v=onepage&q=%22A%20tectonic%20approach%20to%20energy%20renovation%20of%20dwellings%20-%20The%20case%20of%20Gellerup%22&f=false>

Catala Bustos, L, Kirkegaard, PH, Sonne-Frederiksen, PF & Buthke, J 2019, A tectonic methodology for timber joints: the excellence of detail in the era of technology throughout an experimental inverstigation. in P J. S. Cruz (ed.), Structures and architecture: Bridging the Gap and Crossing Borders - Proceedings of the 4th International Conference on Structures and Architecture, ICSA 2019. CRC Press, Leiden, pp. 237-244. https://doi.org/10.1201/9781315229126-29

Anwar, S , Prasad, R, Chowdhry, BS & Anjum, MR 2019, 'A Telemedicine Platform for Disaster Management and Emergency Care', Wireless Personal Communications, vol. 106, no. 1, pp. 191-204. https://doi.org/10.1007/s11277-019-06273-6

Bundsgaard, J, ATEL– Kodning af elevtekster..., 2019, Computer programme. <https://atel.au.dk/>

Rini, BI, Powles, T, Atkins, MB, Escudier, B, McDermott, DF, Suarez, C, Bracarda, S, Stadler, WM, Donskov, F, Lee, JL, Hawkins, R, Ravaud, A, Alekseev, B, Staehler, M, Uemura, M, De Giorgi, U, Mellado, B, Porta, C, Melichar, B, Gurney, H, Bedke, J, Choueiri, TK, Parnis, F, Khaznadar, T, Thobhani, A, Li, S, Piault-Louis, E, Frantz, G, Huseni, M, Schiff, C, Green, MC & Motzer, RJ 2019, 'Atezolizumab plus bevacizumab versus sunitinib in patients with previously untreated metastatic renal cell carcinoma (IMmotion151): a multicentre, open-label, phase 3, randomised controlled trial', The Lancet, vol. 393, no. 10189, pp. 2404-2415. https://doi.org/10.1016/S0140-6736(19)30723-8

Lind, H 2019, 'At gå håndværkskritisk til værks', Dansk Noter, no. 3, pp. 12-18.

Bayer, K 2019, 'At gøre faste tanker flydende', ATLAS.

Skinnebach, LK 2019, 'At hæve sig over tidens kostbare dråber: tiden og evigheden i senmiddelalderens billeder', Passepartout, no. 39, pp. 31-46. <https://tidsskrift.dk/passepartout/article/download/118788/166654/246448>

Louw, ME 2019, 'Atheism 2.0. Searching for spaces for atheism in contemporary Kyrgyzstan', Central Asian Affairs, vol. 6, no. 2-3, pp. 206-223. https://doi.org/10.1163/22142290-00602007

Welz, C 2019, 'A Theological Phenomenology of Listening: God’s ‘Voice’ and ‘Silence’ after Auschwitz', Religions, vol. 139, no. 10. https://doi.org/10.3390/rel10030139

Szatkowski, J 2019, A theory of dramaturgy. Routledge. https://doi.org/10.4324/9781351132114

Karpova, Y 2019, ‘A thing of quality defies being produced in quantity’: Suprematist Porcelain and its Afterlife in Leningrad Design. in C Lodder (ed.), Celebrating Suprematism: New Approaches to the Art of Kazimir Malevich. Brill, pp. 198-220. https://doi.org/10.1163/9789004384989

Stévart, T, Dauby, G, Lowry, P, Blach-Overgaard, A, Droissart, V, Harris, DJ, Mackinder, AB, Schatz, GE, Sonké, B, Sosef, MSM, Svenning, JC, Wieringa, J & Couvreur, TLP 2019, 'A third of the tropical African flora is potentially threatened with extinction', Science Advances, vol. 5, no. 11, eaax9444. https://doi.org/10.1126/sciadv.aax9444

Bruun, H 2019, 'A Third Television Paradigm in Production: Public Service Television in the Digital Era', Paper presented at Screen Industries in East-Central Europe, Prag, Czech Republic, 22/11/2019 - 23/11/2019.

Gleaves, J & Christiansen, AV 2019, 'Athletes¹ perspectives on WADA and the Code: a review and analysis', International Journal of Sport Policy and Politics, vol. 11, no. 2, pp. 341-353. https://doi.org/10.1080/19406940.2019.1577901

Chen, K-L & Møller, C 2019, 'A thousand-mile journey begins with the very first step: The case of a product-centric manufacturing firm’s transformation towards servitization', CIRP Journal of Manufacturing Science and Technology, vol. 27, pp. 102-113. https://doi.org/10.1016/j.cirpj.2019.06.002

Gabriel Rios-Kristjansson, J, Rizo-Roca, D, Mist Kristjansdottir, K, Andres Nunez-Espinosa, C, Ramon Torrella, J, Pages, T & Viscor, G 2019, 'A three-criteria performance score for rats exercising on a running treadmill', PLoS One, vol. 14, no. 7, 0219167. https://doi.org/10.1371/journal.pone.0219167

Hansen, BB 2019, 'At identificere med symptomet', Paper presented at Symptomet! Årsmøde for Selskab for Teoretisk Psykoanalyse, 06/12/2019 - 07/12/2019.

Hansen, BB 2019, 'At kalde til orden', Dagbladet Information.

Rabjerg, B 2019, 'At K.E. Løgstrup skulle have haft et bankende hjerte for Hitler, førerskab og nazismen, kan meget klart afvises', Kristeligt Dagblad, pp. 11. <https://www.kristeligt-dagblad.dk/debatindlaeg/leder-af-loegstrup-arkiv-ke-loegstrup-skulle-have-haft-et-bankende-hjerte-hitler>

Møller, RB & Sørensen, JK 2019, At lære at gribe og begribe. in RB Møller & JK Sørensen (eds), Lidenskab og livskvalitet gennem idræt. Aarhus Universitetsforlag, Aarhus, pp. 161-175.

Nielsen, K 2019, At lære gennem deltagelse. in P Brodersen (ed.), Det lærende menneske: At lære på forskellige måder. Hans Reitzels Forlag, København, pp. 143-161.

Christensen, G & Wiberg, M 2019, At lære gennem erfaring og tænkning. in P Brodersen (ed.), Det lærende menneske: seks tilgange til pædagogisk praksis. Hans Reitzels Forlag, København, pp. 27-47.

Keiding, TB & Thingholm, HB 2019, At lære gennem konstruktioner. in P Brodersen (ed.), Det lærende menneske: Seks tilgange til pædagogisk praksis. Hans Reitzels Forlag, Kbh., pp. 49-72.

Wahlgren, B 2019, At lære som voksen. in H Duch (ed.), På diplom: at lære som voksen. Gyldendal, København, pp. 27-38.

Welz, C 2019, At lytte til den indre stemme: Kierkegaard, Luther og Hannah Arendt. in M Rasmussen (ed.), Mennesket og det andet: Bidrag til den eksistentielle fænomenologi. Aalborg Universitetsforlag, Aalborg, pp. 111-131.

Ellermann, T , Bossi, R , Nygaard, J , Christensen, JH , Løfstrøm, P , Monies, C , Grundahl, L , Geels, C , Nielsen, IE & Poulsen, MB 2019, Atmosfærisk deposition 2017: NOVANA. Videnskabelig rapport fra DCE - Nationalt Center for Miljø og Energi, no. 304, Aarhus University, DCE - Danish Centre for Environment and Energy, Aarhus. <https://dce2.au.dk/pub/SR304.pdf>

Ellermann, T , Bossi, R , Nygaard, J , Christensen, JH , Løfstrøm, P , Monies, C , Geels, C , Nielsen, IE & Poulsen, MB 2019, Atmosfærisk deposition 2018: NOVANA. Videnskabelig rapport fra DCE - Nationalt Center for Miljø og Energi, no. 351, Aarhus Universitet, Aarhus.

Silva, AG, Li, Z, Morgen, P & Pedersen, K 2019, 'Atomically controlled growth of high current interest functional semiconductor systems', 21st International Vacuum Congress, Malmö, Sweden, 01/07/2019 - 05/07/2019.

Li, Y , Li, Z, Ahsen, A, Lammich, L, Mannie, GJA, Niemantsverdriet, JWH & Lauritsen, JV 2019, 'Atomically Defined Iron Carbide Surface for Fischer-Tropsch Synthesis Catalysis', ACS Catalysis, vol. 9, no. 2, pp. 1264-1273. https://doi.org/10.1021/acscatal.8b03684

Karamatskos, ET, Goldsztejn, G, Raabe, S, Stammer, P, Mullins, T, Trabattoni, A, Johansen, RR , Stapelfeldt, H, Trippel, S, Vrakking, MJJ, Küpper, J & Rouzée, A 2019, 'Atomic-resolution imaging of carbonyl sulfide by laser-induced electron diffraction', Journal of Chemical Physics, vol. 150, no. 24, 244301. https://doi.org/10.1063/1.5093959

Li, Y , Adamsen, KC , Lammich, L , Lauritsen, JV & Wendt, S 2019, 'Atomic-Scale View of the Oxidation and Reduction of Supported Ultrathin FeO Islands', ACS Nano, vol. 13, no. 10, pp. 11632-11641. https://doi.org/10.1021/acsnano.9b05470

Jorgensen, MS , Mortensen, HL , Meldgaard, SA , Kolsbjerg, EL , Jacobsen, TL , Sorensen, KH & Hammer, B 2019, 'Atomistic structure learning', Journal of Chemical Physics, vol. 151, no. 5, 054111. https://doi.org/10.1063/1.5108871

Mencke, I, Omigie, D, Wald-Fuhrmann, M & Brattico, E 2019, 'Atonal Music: Can Uncertainty Lead to Pleasure?', Frontiers in Neuroscience, vol. 12, no. 979, 979. https://doi.org/10.3389/fnins.2018.00979

Aagaard, A , Presser, M , Beliatis, M, Mansour, H & Nagy, S 2019, A Tool for Internet of Things Digital Business Model Innovation. in 2018 IEEE Globecom Workshops, GC Wkshps 2018 - Proceedings., 8644517, IEEE, 2018 IEEE Globecom Workshops, GC Wkshps 2018, Abu Dhabi, United Arab Emirates, 09/12/2018. https://doi.org/10.1109/GLOCOMW.2018.8644517

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Lise Refstrup Linnebjerg Pedersen