Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Østergaard, LG, Riis-Djernæs, LM & Jørgensen, SS 2019, Ergoterapeutisk somatisk rehabilitering til voksne og ældre. in Å Brandt, H Peoples & U Pedersen (eds), Basisbog i ergoterapi. 4 edn, Ergo/Munksgaard, København, pp. 289.

Pilegaard, MS, la Cour, K & Peoples, H 2019, Ergoterapi inden for det palliative område. in Å Brandt, H Peoples & U Pedersen (eds), Basisbog i ergoterapi. 4. edn, Munksgaard , pp. 373-388.

Arboe, T 2019, 'Er Harboøremål Thybomål?', Årsskrift for Foreningen Thybomål, vol. 2019, pp. 19-28.

Iversen, AK 2019, 'Erindringens kontemporaneitet', Passepartout, no. 39, pp. 127-142. <https://tidsskrift.dk/passepartout/article/download/118796/166661/>

Huggler, J 2019, 'Erkendelse i undervisningssammenhæng ifølge K. Grue-Sørensen', Studier i Pædagogisk Filosofi, vol. 7, no. 1, pp. 74-98. https://doi.org/10.7146/spf.v7i1.106507

Gregersen, AB 2019, 'Er kristendommen europæisk?', ATLAS, vol. 4, pp. 36-37. <https://atlasmag.dk/kultur/b%C3%B8ger/er-kristendommen-europ%C3%A6isk>

Binderkrantz, AS 2019, 'Er kvinder dårligere undervisere end mænd - ifølge de studerende?', Politica - Tidsskrift for politisk videnskab, vol. 51, no. 1, pp. 102-119. <http://politica.dk/fileadmin/politica/Dokumenter/politica_51_1/binderkrantz.pdf>

Zhou, P, Braud, T, Alhilal, A, Hui, P & Kangasharju, J 2019, ERL: Edge Based Reinforcement Learning for Optimized Urban Traffic Light Control. in 2019 IEEE International Conference on Pervasive Computing and Communications Workshops, PerCom Workshops 2019., 8730706, IEEE, pp. 849-854, 2019 IEEE International Conference on Pervasive Computing and Communications Workshops, PerCom Workshops 2019, Kyoto, Japan, 11/03/2019. https://doi.org/10.1109/PERCOMW.2019.8730706

Ejersbo, LR 2019, 'Er matematik retfærdigt?', Folkeskolen. <https://www.folkeskolen.dk/659042/er-matematik-retfaerdigt>

Ejersbo, LR 2019, 'Er nogle regnestrategier bedre end andre?', Folkeskolen. <https://www.folkeskolen.dk/1103801/er-nogle-regnestrategier-bedre-end-andre>

Jandl, G, Baum, C, Heckrath, GJ , Greve, MH, Kanal, A, Mander, Ü, Maliszewska-Kordybach, B, Niedzwiecki, J, Eckhardt, K-U & Leinweber , P 2019, 'Erosion Induced Heterogeneity of Soil Organic Matter in Catenae from the Baltic Sea Catchment', Soil systems, vol. 3, no. 2, 42, pp. 1-29. https://doi.org/10.3390/soilsystems3020042

Rodriguez-Mozos, JM & Moya, A 2019, 'Erosion of an exoplanetary atmosphere caused by stellar winds', Astronomy & Astrophysics (A&A), vol. 630, 52. https://doi.org/10.1051/0004-6361/201935543

Jansen, JD , Knudsen, MF , Andersen, JL, Heyman, J & Egholm, DL 2019, 'Erosion rates in Fennoscandia during the past million years', Quaternary Science Reviews, vol. 207, pp. 37-48. https://doi.org/10.1016/j.quascirev.2019.01.010

Stange, KM, Midtkandal, I, Nystuen, JP, Murray, A, Sohbati, R, Thompson, W, Spiegel, C & Kuss, HJ 2019, 'Erosive response of non-glaciated pyrenean headwater catchments to the last major climate transition and establishing interglacial conditions', Quaternary, vol. 2, no. 2, 17. https://doi.org/10.3390/quat2020017

Vase, L 2019, 'Er placebokontrol af operationer kontroversielt eller essentielt?', Ugeskrift for Læger, vol. 181, no. 8, V70470, pp. 757.

Koch, BEV, Yang, S, Lamers, G, Stougaard, J & Spaink, HP 2019, 'Erratum: Author Correction: Intestinal microbiome adjusts the innate immune setpoint during colonization through negative regulation of MyD88 (Nature communications (2018) 9 1 (4099))', Nature Communications, vol. 10, no. 1, 526. https://doi.org/10.1038/s41467-019-08456-y

Vinding, MS, Guérin, B, Vosegaard, T & Chr. Nielsen, N 2019, 'Erratum to: Local SAR, global SAR, and power-constrained large-flip-angle pulses with optimal control and virtual observation points: SAR Constrained Large-Flip-Angle Pulses (Magnetic Resonance in Medicine, (2017), 77, 1, (374-384), 10.1002/mrm.26086)', Magnetic Resonance in Medicine, vol. 81, no. 3, pp. 2195-2195. https://doi.org/10.1002/mrm.27581

Nielsen, DU , Hu, XM , Daasbjerg, K & Skrydstrup, T 2019, 'Erratum to: Chemically and electrochemically catalysed conversion of CO2 to CO with follow-up utilization to value-added chemicals (Nature Catalysis, (2018), 1, 4, (244-254), 10.1038/s41929-018-0051-3)', Nature Catalysis, vol. 2, no. 1, pp. 95. https://doi.org/10.1038/s41929-018-0152-z

Clough, RE, Barillà, D, Delsart, P, Ledieu, G, Spear, R, Crichton, S, Vehier, CM, Peacock, J, Sobocinski, J & Haulon, S 2019, 'Erratum to ‘Long-term Survival and Risk Analysis in 136 Consecutive Patients With Type B Aortic Dissection Presenting to a Single Centre Over an 11-year Period’ (European Journal of Vascular & Endovascular Surgery (2019) 57(5) (633–638), (S1078588418306427), (10.1016/j.ejvs.2018.08.042))', European Journal of Vascular and Endovascular Surgery, vol. 58, no. 6, pp. 944. https://doi.org/10.1016/j.ejvs.2019.09.506

Nørskov, KH, Overgaard, D, Lomborg, K , Kjeldsen, L & Jarden, M 2019, 'Erratum to “Patients experiences and social support needs following the diagnosis and initial treatment of acute leukemia - A qualitative study” [Eur. J. Oncol. Nurs. 41 (2019) 49–55](S1462388919300535)(10.1016/j.ejon.2019.05.005)', European Journal of Oncology Nursing, vol. 42, pp. 162. https://doi.org/10.1016/j.ejon.2019.08.004

Shcherbakova, OV, Filippova, MG & Shtyrov, YY 2019, 'ERROR-RELATED NEGATIVITY AS A MARKER OF UNCONSCIOUS SENSITIVITY TO AMBIGUITY', Zhurnal Vysshei Nervnoi Deyatelnosti Imeni I.P. Pavlova, vol. 69, no. 3, pp. 334-338. https://doi.org/10.1134/S0044467719030146

Isager, H 2019, Erstatningsansvar for manglende tilsyn med børn. in T Iversen, S Karstoft & LHG Madsen (eds), Festskrift til Bent Iversen. Djøf Forlag, Kbh., pp. 507-519.

Graven Nielsen, K 2019, Erstatningsansvar ved prisfastsættelse af andelsboliger - set fra bestyrelsens perspektiv. Karnov Group.

Jensen, MB , Lehmann, JO , Mogensen, L & Vaarst, M 2019, Er tidlig adskillelse af ko og kalv mest skånsomt?.

Riisager, E (ed.) & Grundtvig, NFS 2019, Er Troen virkelig en Skole-Sag? Grundtvigs Værker. <http://grundtvigsværker.dk/tekstvisning/19707/0#{"0":0,"v0":0,"k":0}>

Tudek, A , Schmid, M & Jensen, TH 2019, 'Escaping nuclear decay: the significance of mRNA export for gene expression', Current Genetics, vol. 65, no. 2, pp. 473-476. https://doi.org/10.1007/s00294-018-0913-x

Gordon, AV , Rohrbeck, R & Schwarz, JO 2019, 'Escaping the 'Faster Horses' Trap: Bridging Strategic Foresight and Design-Based Innovation', Technology Innovation Management Review, vol. 9, no. 8, pp. 30-42. https://doi.org/10.22215/timreview/1259

Cordoves Santiesteban, A 2019, Escola e transformação social: a (re)configuração da educação cubana após 1959. in Antropologia e Educação: refletindo sobre processos educativos em contextos escolares, não escolares e de políticas públicas. Copiart-EDUA-EDUFRN, Tubarão, pp. 199. https://doi.org/10.5281/zenodo.13345334

Madonna, R, Van Laake, LW, Botker, HE, Davidson, SM, De Caterina, R, Engel, FB, Eschenhagen, T, Fernandez-Aviles, F, Hausenloy, DJ, Hulot, J-S, Lecour, S, Leor, J, Menasché, P, Pesce, M, Perrino, C, Prunier, F, Van Linthout, S, Ytrehus, K, Zimmermann, W-H, Ferdinandy, P & Sluijter, JPG 2019, 'ESC Working Group on Cellular Biology of the Heart: Position paper for Cardiovascular Research: Tissue engineering strategies combined with cell therapies for cardiac repair in ischaemic heart disease and heart failure', Cardiovascular Research, vol. 115, no. 3, pp. 488-500. https://doi.org/10.1093/cvr/cvz010

Nielsen, AH , Angel, S , Hansen, TB & Egerod, I 2019, 'ESICM LIVES 2019: Relatives' diaries for critically ill patients prevents distress in relatives. A mixed methods analysis', Intensive Care Medicine Experimental, vol. 7, 000154, pp. 248-248. https://doi.org/10.1186/s40635-019-0265-y

Pereira, VS , Joca, SRL, Harvey, BH, Elfving, B & Wegener, G 2019, 'Esketamine and Rapastinel, but not Imipramine, have antidepressant-like effect in a treatment-resistant animal model of depression', Acta Neuropsychiatrica, vol. 31, no. 5, pp. 258-265. https://doi.org/10.1017/neu.2019.25

Ringgaard, VK , Wemmelund, KB , Sloth, E & Juhl-Olsen, P 2019, 'Esmolol does not affect circulation negatively during resuscitation', American Journal of Emergency Medicine, vol. 37, no. 4, pp. 690-695. https://doi.org/10.1016/j.ajem.2018.07.023

Lievens, Y, Audisio, R, Banks, I, Collette, L, Grau, C, Oliver, K, Price, R & Aggarwal, A 2019, 'ESMO-MCBS: setting the record straight – Authors' reply', The Lancet Oncology, vol. 20, no. 4, pp. e193. https://doi.org/10.1016/S1470-2045(19)30166-4

Jiang, H , Zhao, J , Liao, D, Wang, G & Gregersen, H 2019, 'Esophageal stress softening recovery is altered in STZ-induced diabetic rats', Journal of Biomechanics, vol. 92, pp. 126-136. https://doi.org/10.1016/j.jbiomech.2019.05.042

Skjærbæk, C , Knudsen, K , Horsager, J & Borghammer, P 2019, 'Esophageal transit and intestinal dysfunction in Parkinson’s disease', Kongres for Medicinsk Studenterforskning, Sønderborg, Denmark, 21/03/2019 - 24/03/2019.

Skjærbæk, C , Knudsen, K , Horsager, J & Borghammer, P 2019, 'Esophageal transit and intestinal dysfunction in Parkinson’s disease', Neuroscience Day 2019, Aarhus, Denmark, 13/05/2019 - 13/05/2019.

Frandsen, K 2019, 'E-sport: media becoming sport', Paper presented at IAMCR 2019, Madrid, Spain, 07/07/2019 - 11/07/2019.

Hansen, BB 2019, 'Essay: Det foruroligende hotel', Politiken.

Andersen, CBH 2019, 'Essays in Health Economics', Aarhus.

Jensen, TM 2019, 'Essays on Dynamic Term Structure Models', Aarhus.

Poulsen, SM 2019, 'Essays on Mixed Service Delivery - Concurrent Sourcing in the Public Sector', Aarhus.

Fluchtmann, J 2019, 'Essays on the economics of job search', Aarhus.

Rømer, TA 2019, 'Essentializing postmodernity', Educational Philosophy and Theory, vol. 50, no. 14, pp. 1598-99. https://doi.org/10.1080/00131857.2018.1462460, https://doi.org/10.1080/00131857.2018.1462460

Christensen, EM, Simpson, GL & Ernest, SKM 2019, 'Established rodent community delays recovery of dominant competitor following experimental disturbance', Proceedings of the Royal Society B: Biological Sciences, vol. 286, no. 1917, 20192269. https://doi.org/10.1098/rspb.2019.2269

Brügger, N, Laursen, D & Nielsen, J 2019, Establishing a corpus of the archived web: The case of the Danish web from 2005 to 2015. in N Brügger & D Laursen (eds), The historical web and Digital Humanities: The case of national web domains. Routledge, Abingdon, pp. 124-142.

van Luijk, JAKR, Popa, M, Swinkels, J, Menon, JML, Alkema, W, Roeleveld, N, Hoffmann, SE, Schlünssen, V, Mandrioli, D, Ritskes-Hoitinga, M & Scheepers, PTJ 2019, 'Establishing a health-based recommended occupational exposure limit for nitrous oxide using experimental animal data: A systematic review protocol', Environmental Research, vol. 178, 108711. https://doi.org/10.1016/j.envres.2019.108711

Amele, S, Peters, L, Sluzhynska, M, Yakovlev, A, Scherrer, A, Domingo, P, Gerstoft, J, Viard, JP, Gisinger, M, Flisiak, R, Bhaghani, S, Ristola, M, Leen, C, Jablonowska, E, Wandeler, G, Stellbrink, H, Falconer, K, D'Arminio Monforte, A, Horban, A, Rockstroh, JK, Lundgren, JD, Mocroft, A & EuroSIDA Study Group 2019, 'Establishing a hepatitis C continuum of care among HIV/hepatitis C virus-coinfected individuals in EuroSIDA', HIV Medicine, vol. 20, no. 4, pp. 264-273. https://doi.org/10.1111/hiv.12711

Bager-Elsborg, A & Greve, L 2019, 'Establishing a Method for Analysing Metaphors in Higher Education Teaching: A case from business management teaching', Higher Education Research and Development, vol. 38, no. 7, pp. 1329-1342. https://doi.org/10.1080/07294360.2017.1327945

Svenningsen, EB & Poulsen, TB 2019, 'Establishing cell painting in a smaller chemical biology lab – A report from the frontier', Bioorganic and Medicinal Chemistry, vol. 27, no. 12, pp. 2609-2615. https://doi.org/10.1016/j.bmc.2019.03.052

Vieira Macêdo, W, Diniz Santos, CE, de Bello Solcia Guerrero, R, Sakamoto, I, Cavalcante Amorim, EL, Bessa Azecedo, E & Damianovic, MHRZ 2019, 'Establishing simultaneous nitrification and denitrification under continuous aeration for the treatment of multi-electrolytes saline wastewater.', Bioresource Technology, vol. 288, 121529. https://doi.org/10.1016/j.biortech.2019.121529

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