Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Zuo, Z, Wu, S, Qi, X & Dong, R 2015, 'Performance enhancement of leaf vegetable waste in two-stage anaerobic systems under high organic loading rate: Role of recirculation and hydraulic retention time', Applied Energy, vol. 147, pp. 279-286. https://doi.org/10.1016/j.apenergy.2015.03.001

Maslim, R, Chaoyi, H, Yixi, Z, Linhao, J, Bahadir Kocer, B & Kayacan, E 2015, Performance evaluation of adaptive and nonadaptive fuzzy structures for 4D trajectory tracking of quadrotors: A comparative study. in Fuzzy Systems (FUZZ-IEEE), 2015 IEEE International Conference on. IEEE, pp. 1-7, 2015 IEEE International Conference on Fuzzy Systems , Istanbul, Turkey, 02/08/2015. https://doi.org/10.1109/FUZZ-IEEE.2015.7337945

Marker, PA, Foged, N, He, X, Christiansen, AV , Refsgaard, JC , Auken, E & Bauer-Gottwein, P 2015, 'Performance evaluation of groundwater model hydrostratigraphy from airborne electromagnetic data and lithological borehole logs', Hydrology and Earth System Sciences, vol. 19, no. 9, pp. 3875-3890. https://doi.org/10.5194/hess-19-3875-2015

Staniok, CD 2015, 'Performance Implications of Goal Prioritization and Commitment: An Empirical Test of the Link between Internal Management and Organizational Performance', Paper presented at Transatlantic Dialogue, Boston, United States, 03/06/2015 - 05/06/2015.

Bækgaard, M 2015, 'Performance Information and Citizen Service Attitudes: Do Cost Information and Service Use Affect the Relationship?', International Public Management Journal, vol. 18, no. 2, pp. 228-245. https://doi.org/10.1080/10967494.2015.1022676

Nielsen, PA & Bækgaard, M 2015, 'Performance Information, Blame Avoidance, and Politicians’ Attitudes to Spending and Reform: Evidence From an Experiment', Journal of Public Administration Research and Theory, vol. 25, no. 2, pp. 545-569. https://doi.org/10.1093/jopart/mut051

Huber, G 2015, 'Performance measurement effects on organizational responses to threats', Measuring Business Excellence, vol. 19, no. 1, pp. 24-32. https://doi.org/10.1108/MBE-11-2014-0048

Hvidman, U 2015, 'Performance measurement in the public sector: Impacts on public performance and citizens' perceptions', Aarhus.

Vestergaard, M , Mikkelsen, A , Bjerring, M, Spleth, P & Kargo, M 2015, Performance of cressbred Holstein bulls and heifer calves slaughtered at 12-month of age. in Book of Abstract of the 66th Annual Meeting of the European Federation of Animal Science. vol. 21, The Netherlands, EAAP Book of Abstracts, pp. 241-241, 66th Annual Meeting of the European Federation of Animal Science, Warsaw, Poland, 31/08/2015. https://doi.org/10.3920/978-90-8686-816-2

Ferris, C, Purcell, PJ, Gordon, AW, Larsen, T & Vestergaard, M 2015, Performance of Holstein-Friesian and '3-breed' crossbred (Swedish-Red x (Jersey/Holstein-Friesian)) dairy cows within a low and moderate concentrate input grassland based system over a full lactation: Delivrable 2.5 Official deliverable of the 7th Framework Programme KBBE. in Adaptation of dairy cattle and goat breeds to organic and low input production systems. pp. 43-64.

Zhou, K , Jensen, AL , Bochtis, D & Sørensen, CAG 2015, 'Performance of machinery in potato production in one growing season', Spanish Journal of Agricultural Research, vol. 13. https://doi.org/10.5424/sjar/2015134-7448

Guzman, JJ, O. Pehlivaner Kara, M, Frey, MM & Angenent, L 2015, 'Performance of polyacrylonitrile (PAN)-based electrospun materials coated in PEDOT conductive polymer as electrodes for bioelectrochemical systems', ISMET-2015 , Phoenix, United States, 01/10/2015 - 04/10/2015.

Rubæk, GH 2015, Performance of selected soil P tests in proficiency test programmes. in Validity and analytical robustness of the Olsen soil P test and other agronomic soil P tests used in the northern Europe. DCA - Nationalt Center for Fødevarer og Jordbrug, DCA rapport, no. 071.

Ørntoft, M-BW, Nielsen, HJ, Ørntoft, TF & Andersen, CL 2015, 'Performance of the colorectal cancer screening marker Sept9 is influenced by age, diabetes and arthritis: a nested case-control study', BMC Cancer, vol. 15/819, pp. 1-10. <http://bmccancer.biomedcentral.com/articles/10.1186/s12885-015-1832-6>

Nielsen, TR & Vedel, KA 2015, 'Performanceuddannelse i Danmark? Muligheder og Udfordringer', Peripeti - tidsskrift for dramaturgiske studier, no. 23, pp. 121-126.

Krøgholt, I 2015, 'Performanceuddannelse mellem overskridelse og institutionalisering', Peripeti, no. 23, pp. 136 - 144.

Toft-Nielsen, C 2015, 'Performing gaming expertise: Maintaining social relationships and doing gender within the context of gamers' daily lives'.

Tietze, A , Mouridsen, K, Lassen-Ramshad, Y & Østergaard, L 2015, 'Perfusion MRI derived indices of microvascular shunting and flow control correlate with tumor grade and outcome in patients with cerebral glioma', PLoS One, vol. 10, no. 4. https://doi.org/10.1371/journal.pone.0123044

Jorge, ÉG, Tanomaru-Filho, M, Guerreiro-Tanomaru, JM, Reis, JMDSN, Spin-Neto, R & Gonçalves, M 2015, 'Periapical repair following endodontic surgery: two- and three-dimensional imaging evaluation methods', Brazilian Dental Journal, vol. 26, no. 1, pp. 69-74. https://doi.org/10.1590/0103-6440201300252

Farup, J, De Lisio, M, Rahbek, SK, Bjerre, J, Vendelbo, MH, Boppart, MD & Vissing, K 2015, 'Pericyte Response to Contraction Mode-Specific Resistance Exercise Training in Human Skeletal Muscle', Journal of Applied Physiology, vol. 119, no. 10, pp. 1053-1063. https://doi.org/10.1152/japplphysiol.01108.2014

Pessoa, R, Sousa, R, Pereira, L, Neves, F, Bezerra, F, Jaecques, S, Sloten, JV, Quirynen, M, Teughels, W & Spin-Neto, R 2015, 'Periimplant bone remodeling around external hexagon and Morse-taper implant connections –prospective randomized controlled split-mouth clinical trial', Clinical Oral Implants Research, vol. 26, no. Supplement S12, pp. 376-376. <http://onlinelibrary.wiley.com/doi/10.1111/clr.367_12679/full>

Schou, S, Koldsland, OC, Saaby, M, Berglundh, T & Isidor, F 2015, Periimplantitis. Et stort klinisk problem? in P Holmstrup (ed.), Aktuel Nordisk Odontologi 2015. 40. årgang edn, Munksgaard , pp. 171-184.

Kristiansen, MK, Joensen, BS, Ekelund, CK, Petersen, OB , Sandager, P & with the Danish Fetal Medicine Study Group 2015, 'Perinatal outcome after first-trimester risk assessment in monochorionic and dichorionic twin pregnancies: a population-based register study', B J O G, vol. 122, no. 10, pp. 1362-69. https://doi.org/10.1111/1471-0528.13326

Ozgur, K, Berkkanoglu, M, Bulut, H, Humaidan, P & Coetzee, K 2015, 'Perinatal outcomes after fresh versus vitrified-warmed blastocyst transfer: retrospective analysis', Fertility and Sterility, vol. 104, no. 4, pp. 899-907. https://doi.org/10.1016/j.fertnstert.2015.06.031

Sørensen, P & Børgesen, CD 2015, 'Periodisering af effekten ved trinvis udfasning af normreduktionen', no. 1563150, 5 p., Nov 23, 2015..

Grønkjær, LL 2015, 'Periodontal disease and liver cirrhosis: A systematic review', SAGE Open Medicine, vol. 3, pp. 2050312115601122. https://doi.org/10.1177/2050312115601122

López, R & Baelum, V 2015, 'Periodontal disease classifications revisited', European Journal of Oral Sciences, vol. 123, pp. 385-389. https://doi.org/10.1111/eos.12227

Klintebjerg, K, Petersen, E, Pshenichnaya, NY, Ermakova, LA, Nagorny, SA & Larsen, CS 2015, 'Periorbital Dirofilaria repens imported to Denmark: A human case report', IDCases, vol. 2, no. 1, pp. 25-6. https://doi.org/10.1016/j.idcr.2014.12.001

Tankisi, H , Pugdahl, K , Rasmussen, MM , Clemmensen, D , Rawashdeh, YFH , Christensen, P , Krogh, K & Fuglsang-Frederiksen, A 2015, 'Peripheral nervous system involvement in chronic spinal cord injury', Muscle & Nerve, vol. 52, no. 6, pp. 1016-1022. https://doi.org/10.1002/mus.24644

Camargo, LL, Denadai-Souza, A, Yshii, LM, Mesquita, FPN, Soares, AG, Lima, C, Schenka, A, Grant, A, Fernandes, E, Muscará, MN & Costa, SKP 2015, 'Peripheral Neurokinin-1 Receptors Contribute to Kaolin-Induced Acute Monoarthritis in Rats', NeuroImmunoModulation, vol. 22, no. 6, pp. 373-384. https://doi.org/10.1159/000381549

d'Amore, F, Gaulard, P, Trümper, L, Corradini, P, Kim, W-S, Specht, L, Bjerregaard Pedersen, M, Ladetto, M & ESMO Guidelines Committee 2015, 'Peripheral T-cell Lymphomas: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up', Annals of Oncology, vol. 26 Suppl 5, pp. v108-15. https://doi.org/10.1093/annonc/mdv201

D'Amore, FA & Pedersen, MB 2015, Peripheral T-cell lymphomas. Book chapter in "lymphomas - Essentials for Clinicians. in Lymphomas - Essentials for Clinicians. ESMO Press, pp. 61-66.

Verwaal, V 2015, Peritoneal metastase and peritoneal mesothelioma. in Cancer principles & Practise of Oncology. 10 edn, Wolters Kluwer Health, pp. 1761.

Jensen, FA , Bonde, L , Kim, S & Boedtkjer, E 2015, 'Perivascular tissue inhibits rho-kinase-dependent smooth muscle Ca²⁺-sensitivity and endothelium-dependent H₂S signalling in rat coronary arteries', The Journal of Physiology, pp. 4747-4764.

Sonne, O 2015, 'Per Jørgensen: Kiropraktikkens historie i Danmark. Syddansk Universitetsforlag, 2014.', Dansk Medicinhistorisk Årbog, vol. 43, pp. 195-197.

Larsen, SN 2015, 'Perkernes sproglige kamp: anmeldelse af Tobias Cadin Borup og Ali Sufi: Gade/dansk ordbog – en håndbog i ghettodansk', Information, pp. 28-29.

Larsen, AH 2015, Per Kirkeby: Malerier 1978-1989. vol. Bd. 2, 1. udgave edn, Systime, Aarhus.

Hollesen, J, Matthiesen, H, Møller, AB & Elberling, B 2015, 'Permafrost thawing in organic Arctic soils accelerated by ground heat production', Nature Climate Change, vol. 5, no. 6, pp. 574-578. https://doi.org/10.1038/nclimate2590

Croix, S 2015, 'Permanency in Early Medieval Emporia: Reassessing Ribe', European Journal of Archaeology, vol. 18, no. 3, pp. 497-523.

Boix, PP, Agarwala, S, Ming Koh, T, Mathews, N & G. Mhaisalkar, S 2015, 'Perovskite Solar Cells: Beyond Methylammnonium lead iodide', The Journal of Physical Chemistry Letters, vol. 6, no. 5, pp. 898-907. https://doi.org/10.1021/jz502547f

Gorbahn, K 2015, Perpetrators, vicitim, heroes: The Second World War and National Socialism in Danish history magazines. in S Popp, J Schumann & M Hannig (eds), Commercialised History: Popular History Magazines in . Approaches to a Historico-Cultural Phenomenon as a Basis for History Teaching . Peter Lang, pp. 319-334. https://doi.org/10.3726/ 978-3-653-05253-4

Sharma, R, Ahlm, C, Østergaard, LJ, Dowell, A, Tran, C, Thomas, S & Eymin, C 2015, 'Persistence of immunity in healthy adults aged >50 years primed with a hepatitis B vaccine 3 years previously', Human Vaccines & Immunotherapeutics, pp. 0. https://doi.org/10.1080/21645515.2015.1019187

Wilkens, R, Wilson, A, Burns, PN, Ghosh, S & Wilson, SR 2015, 'Persistent Enhancement in Contrast Ultrasound Studies of Severe Crohn's Disease: Stuck Bubbles?', Journal of Crohn's and Colitis, vol. 9, P130, pp. S141-S142.

Presman, B, Finnerup, K, Andresen, SR , Nikolajsen, L & Finnerup, NB 2015, 'Persistent Pain and Sensory Abnormalities after Abdominoplasty', Plastic and Reconstructive Surgery, vol. 3, no. 11, e561. https://doi.org/10.1097/GOX.0000000000000542

Bjørnholdt, KT , Brandsborg, B , Søballe, K & Nikolajsen, L 2015, 'Persistent pain is common 1-2 years after shoulder replacement', Acta Orthopaedica, vol. 86, no. 1, pp. 71-77. https://doi.org/10.3109/17453674.2014.987065

Barré, P, Plante, AF, Cecillon, L, Lutfalla, S, Baudin, F, Bernard, S, Quenea, K, Anquetil, C, Christensen, BT, Houot, S, Kätterer, T, Macdonald, A, van Oort, F & Chenu, C 2015, 'Persistent soil organic carbon is thermally stable low energy organic carbon', 5th International SYMPOSIUM on SOIL ORGANIC MATTER, Göttingen, Germany, 20/09/2015 - 24/09/2015.

Saksø, H , Foldager, CB & Bünger, C 2015, 'Persisting upper cervical pain as sole symptom by unstable fractures in the cervical spine', Ugeskrift for Læger, vol. 177, no. 25.

Steinle, S, Reis, S, Sabel, CE, Semple, S, Twigg, MM, Braban, CF, Leeson, SR, Heal, MR, Harrison, D, Lin, C & Wu, H 2015, 'Personal exposure monitoring of PM2.5 in indoor and outdoor microenvironments', Science of the Total Environment, vol. 508, pp. 383-394. https://doi.org/10.1016/j.scitotenv.2014.12.003

Ridgway, R 2015, 'Personalisation as Currency', A Peer-Reviewed Journal About, vol. 4, no. 1, pp. 17-28.

Vedel, A , Thomsen, DK & Larsen, L 2015, 'Personality, academic majors and performance: Revealing complex patterns', Personality and Individual Differences, vol. 85, pp. 69-76. https://doi.org/10.1016/j.paid.2015.04.030

Displaying results 165301 to 165350 out of 389758

3307