A manuscript with the title “Topological constraints and modular structure in the folding and functional motions of GlpG, an intramembrane protease” has been published in PNAS. This paper uses simulations to investigate the effects of constraining the unfolded ensemble of GlpG in a bilayer on its folding mechanism. Using the detailed folding mechanisms found in this paper, we propose a possible source of the large number of negative phi-values found in our previous experimental work. Otzen lab co-authors include Nicholas P Schafer and Daniel E Otzen. This work is a collaboration with Kresten Lindorff-Larsen at Copenhagen University as well as Ha H Truong and Peter G Wolynes at Rice University.
A manuscript with the title “Cooperative folding of a polytopic α-helical membrane protein involves a compact N-terminal nucleus and nonnative loops” has been published in PNAS. This manuscript describes the most comprehensive phi-value analysis of a membrane protein to-date. Co-authors in the Otzen group include Wojciech Paslawski, Ove K Lillelund, Julie Veje Kristensen, Nicholas P Schafer, and Daniel E Otzen. This work was a collaboration with Rosanna P Baker and Sinisa Urban at Johns Hopkins University.
Nick Schafer will be supported by the Carlsberg Foundation and DFF-FNU for a total of two years to work on a project entitled “Combining experiment and simulation to elucidate folding and stability of the alpha-helical TMP GlpG”.
Daniel is awarded 250.000 kr from “Civilingeniør Frode V. Nyegaard og hustrus Fond” to buy apparatus
for protein purification.
Daniel is awarded 500.000 kr from the Carlsberg Foundation to buy apparatus for
biophysical characterization of proteins. See
Daniel and Lars Kjær are awarded 2.151.608 kr from The Danish Council for Independent Research
| Natural Sciences for the project “Amyloid in plants”. Lars starts work in February 2013 as post-doc
in Daniel’s group from February 2013 for a 2-year period.
Dr. Kell K. Andersen is awarded 3.178.590 DKK from The Danish Council for Independent Research |
Technology and Production Sciences for the project
“Biosurfactants – Environmentally friendly Protein Partners (BEPP)”
which he will carry out together with Daniel Otzen.
Jon Gade successfully defends his PhD thesis
“Aggregation of whey proteins – and their interactions with amphiphiles”.