Publications

Recent publications

López Hernández, M., Scavenius, C., Otzen, D. E. & Pedersen, J. S. (2026). Divergent effects of anionic surfactants on laundry enzymes: Structural stability, activity modulation, and self-cleavage mechanisms. International Journal of Biological Macromolecules, 335(1), Article 149069. https://doi.org/10.1016/j.ijbiomac.2025.149069

Otzen, D. E., Gamon, L. F., Hägglund, P., Nielsen, J., Pedersen, J. N., Nybo, T., Nowak, J. S., Amstrup, S. K., Pirhaghi, M. & Davies, M. J. (2026). Nitrated products formed on α-synuclein are preferentially incorporated into oligomers but excluded from fibrils: A mechanism for accumulation of neurotoxic species. Biochimica et Biophysica Acta - Proteins and Proteomics, 1874(2), Article 141118. https://doi.org/10.1016/j.bbapap.2025.141118

Ramos Docampo, M. A., Abild Meyer, C., Ryberg, C., Otzen, D. E., Hirsch, C. & Städler, B. (2025). Actin Polymerizing Motors to Assist Cytoskeleton-like Networks Formation in Artificial Cells. ACS Nano, 19(42), 37324-37338. https://doi.org/10.1021/acsnano.5c12624

Teixeira, L. M. C., Paiva, P., Ferreira, P., Rotilio, L., Morth, J. P., Otzen, D. E., Fernandes, P. A. & Ramos, M. J. (2025). Bridging experiment and theory: a computational exploration of UMG-SP3 dynamics. Pure and Applied Chemistry, 97(10), 1405-1418. https://doi.org/10.1515/pac-2025-0489

Bendtsen, M. K., Nowak, J. S., Paiva, P., López Hernández, M., Ferreira, P., Pedersen, J. S., Bekker, N. S., Viezzi, E., Bisiak, F., Brodersen, D. E., Pedersen, L. H., Zervas, A., Fernandes, P. A., Ramos, M. J., Stougaard, P., Thøgersen, M. S. & Otzen, D. E. (2025). Cold-Active Starch-Degrading Enzymes from a Cold and Alkaline Greenland Environment: Role of Ca²⁺ Ions and Conformational Dynamics in Psychrophilicity. Biomolecules, 15(3), Article 415. https://doi.org/10.3390/biom15030415

Marzookian, K., Aliakbari, F., Hourfar, H., Sabouni, F., Otzen, D. E. & Morshedi, D. (2025). Corrigendum to "The neuroprotective effect of human umbilical cord MSCs-derived secretome against α-synuclein aggregates on the blood-brain barrier" [Int. J. Biol. Macromol. Volume 290 (2025), 140387]. International Journal of Biological Macromolecules, Article 149909. Advance online publication. https://doi.org/10.1016/j.ijbiomac.2025.149909

Nybo, T., Gamon, L. F., Fuentes-Lemus, E., Otzen, D. E., Davies, M. J. & Hägglund, P. (2025). Dimethyl labeling of N-terminal amines allows unambiguous identification of protein crosslinks. Free Radical Biology & Medicine, 227, 629-637. https://doi.org/10.1016/j.freeradbiomed.2024.12.002

Zhang, T., Bär, J., Risberg, L., Gómez Mejia, A., Hammar, H., Löffler, S., Otzen, D. E., Andreasen, M., Meyer, R. L., Melican, K., Zinkernagel, A. S. & Richter-Dahlfors, A. (2025). Dynamic visualization of extracellular matrix components in S. aureus colony biofilms reveals functional amyloids leading to the formation of cap-like structures. Biofilm, 10, Article 100318. https://doi.org/10.1016/j.bioflm.2025.100318

Jin, Z., Olsen, W. P., Mörman, C., Leppert, A., Kumar, R., Møllebjerg, A., Nielsen, L. G., Moshynets, O. V., Frasinyuk, M. S., Elosua, J. Y., Ferreira, D., Abelein, A., Landreh, M., Knight, S. D., Johansson, J., Otzen, D. E. & Chen, G. (2025). Helicobacter pylori CagA protein is a potent and broad-spectrum amyloid inhibitor. Science Advances, 11(24), Article eads7525. https://doi.org/10.1126/sciadv.ads7525

Chrenková, A., Nashier, P., Madsen, C. L., Singh, M., Nielsen, J., Otzen, D. E., Enghild, J. J., Macek, B., Skjerning, R. B. & Brodersen, D. E. (2025). Homologous HipA-like kinases are controlled by internal translational initiation and genetic organisation. FEBS Letters. Advance online publication. https://doi.org/10.1002/1873-3468.70234

Teixeira, L. M. C., Paiva, P., Johansen, M. B., Sommerfeldt, A., Petersen, A. R., Rotilio, L., Sandahl, A., Morth, J. P., Westh, P., Otzen, D. E., Fernandes, P. A. & Ramos, M. J. (2025). Hydrolysis of the Urethane Bond Catalyzed by Pseudomonas sp. MIS38 Lipase: A QM/MM Mechanistic Insight. ACS Catalysis, 15, 14728-14740. https://doi.org/10.1021/acscatal.5c03228

Gothelf, K. V., Kjems, J., Otzen, D. E., Nielsen, N. C., Hornekær, L., Zelikin, A., Pedersen, J. S., Vorup-Jensen, T. & Sutherland, D. S. (2025). Ingen reel mulighed for medarbejdere for at gøre indsigelse mod ledelsens beslutning om at afvikle iNANO. Omnibus, 2025(Juni). https://omnibus.au.dk/arkiv/vis/artikel/aabent-brev-medarbejdere-er-sat-uden-for-indflydelse-i-ledelsens-beslutning-om-at-afvikle-inano

Otzen, D. E., Peña-Díaz, S., Widmann, J., Daugberg, A. O. H., Zhang, Z., Jiang, Y., Mittal, C., Dueholm, M. K. D., Louros, N., Wang, H. & Javed, I. (2025). Interactions between pathological and functional amyloid: A match made in Heaven or Hell? Molecular Aspects of Medicine, 103, 101351. Article 101351. https://doi.org/10.1016/j.mam.2025.101351

Olsen, W. P., Larsen, A.-K. K., Christensen, J. L., Malle, M. G. & Otzen, D. E. (2025). Investigating strategies for creating cross-linked amyloid fibril networks through branching of amyloid growth. Colloids and surfaces. B, Biointerfaces, 251, 114617. Article 114617. https://doi.org/10.1016/j.colsurfb.2025.114617

López Hernández, M., Otzen, D. E. & Pedersen, J. S. (2025). Investigating the interactions between an industrial lipase and anionic (bio)surfactants. Journal of Colloid and Interface Science, 679(B), 294-306. https://doi.org/10.1016/j.jcis.2024.10.060

Nielsen, J., Pedersen, J. N., Kleijwegt, G., Nowak, J. S., Nami, F., Johansen, C., Sassetti, E., Berg, B. B., Lyngsø, N. M., Brøchner, B. H., Holm Carlson, J., Simonsen, A. J., Pallisgaard Olsen, W., Simonsen, B. W., Mikkelsen, J. H., Sereika-Bejder, V., Lauritsen, J. A., Merrild, K. F., Malle, M. G. ... Otzen, D. E. (2025). Nanobodies raised against the cytotoxic α-synuclein oligomer are oligomer-specific and promote its cellular uptake. npj biosensing, 2, Article 23. https://doi.org/10.1038/s44328-025-00042-1

Peña Díaz, S., Zhang, Z., Jiang, Y., Daugberg, A. O. H., López Hernández, M., Nielsen, J., Dueholm, M. K. D., Dong, MD., Pedersen, J. S., Otzen, D. E. & Wang, H. (2025). Natural Design of a Stabilized Cross-β Fold: Structure of the FuA FapC from Pseudomonas Sp. UK4 Reveals a Critical Role for Stacking of Imperfect Repeats. Advanced Materials, 37(34), Article 2505503. https://doi.org/10.1002/adma.202505503

Mirab, F., Pirhaghi, M., Otzen, D. E. & Saboury, A. A. (2025). Parkinson's disease and gut microbiota metabolites: The dual impact of vitamins and functional amyloids. B B A - Molecular Basis of Disease, 1871(6), Article 167862. https://doi.org/10.1016/j.bbadis.2025.167862

Mishra, A., Golbek, T. W., Thomassen, A. B., Zuzic, L., Schmüser, L., Saeed, K. H., Madzharova, F., Nielsen, J., Schiøtt, B., Otzen, D. E. & Weidner, T. (2025). Pathological Folding of α-Synuclein on Polystyrene Nanoplastic Revealed by Sum Frequency Scattering and 2D Infrared Spectroscopy. The Journal of Physical Chemistry Letters, 16(45), 11893-11900. https://doi.org/10.1021/acs.jpclett.5c02526

Jansen, S. A. H., Dreyer, L. S. A., van Basten, J., Yao, Y., Otzen, D. E., de Greef, T. F. A., Knowles, T. P. J., Meijer, E. W. & Erkamp, N. A. (2025). PhaseXplorer Creates High-Dimensional Phase Diagrams with Closed-Loop Active Learning. ACS Nano, 19(45), 38981-38989. https://doi.org/10.1021/acsnano.5c07268

Nowak, J. S., Kruuse, N., Rasmussen, H. Ø., Tian, P., Astono, J., Schultz-Nielsen, S., Thøgersen, M. S., Stougaard, P., Pedersen, J. S. & Otzen, D. E. (2025). Quaternary stabilization of a GH2 β-galactosidase from the psychrophile A. ikkensis, a flexible and unstable dimeric enzyme. Protein Science, 34(5), Article e70141. https://doi.org/10.1002/pro.70141

Golan, N., Parizat, A., Tabachnikov, O., Barnea, E., Olsen, W. P., Otzen, D. E. & Landau, M. (2025). Resilience and Charge-Dependent Fibrillation of functional amyloid: Interactions of Pseudomonas Biofilm-Associated FapB and FapC Amyloids. The Journal of Biological Chemistry, 301(2), Article 108096. https://doi.org/10.1016/j.jbc.2024.108096

Nowak, J. S., Olesen, S., Tian, P., Bærentsen, R. L., Brodersen, D. E. & Otzen, D. E. (2025). Role of electrostatics in cold adaptation: A comparative study of eury- and stenopsychrophilic triose phosphate isomerase. Biochimica et Biophysica Acta - Proteins and Proteomics, 1873(4), Article 141072. https://doi.org/10.1016/j.bbapap.2025.141072

Bro̷chner, B. V., Zhang, X., Nielsen, J., Kjems, J., Otzen, D. E. & Malle, M. G. (2025). Single-vesicle Tracking of α-Synuclein Oligomers Reveals Pore Formation by a Three-Stage Model. ACS Nano, 19(36), 32108-32122. https://doi.org/10.1021/acsnano.5c04005

Rotilio, L., Bayer, T., Meinert, H., Teixeira, L. M. C., Johansen, M. B., Sommerfeldt, A., Petersen, A. R., Sandahl, A., Keller, M. B., Holck, J., Paiva, P., Otzen, D. E., Bornscheuer, U. T., Wei, R., Fernandes, P. A., Ramos, M. J., Westh, P. & Morth, J. P. (2025). Structural and Functional Characterization of an Amidase Targeting a Polyurethane for Sustainable Recycling. Angewandte Chemie International Edition, 64(7), Article e202419535. https://doi.org/10.1002/anie.202419535

Singh, P., Kadam, N. Y., Panigrahi, R., Mehrotra, A., Upadhayay, K., Dey, M., Tyagi, A., Aquib, M., Nielsen, J., Kleijwegt, G., Singh, P., Sharma, A., Rao, A., Otzen, D. E., Kumar, A. & Sharma, D. (2025). Sulfamerazine as a Potential Modulator against α-Synuclein Aggregation and Associated Toxicity. ACS Chemical Neuroscience, 16(5), 880-894. https://doi.org/10.1021/acschemneuro.4c00803

Peña-Díaz, S., Jiang, Y., Zhang, Z., Daugberg, A., Ferreira, P., López Hernández, M., Mittal, C., Ramos, M. J., Pedersen, J. S., Dueholm, M. K. D., Qin, C., Wang, H. & Otzen, D. E. (2025). The Importance of Being Imperfect: Structure and Function of Bacterial Amyloid. Advanced Science, Article e17090. Advance online publication. https://doi.org/10.1002/advs.202517090

Marzookian, K., Aliakbari, F., Hourfar, H., Sabouni, F., Otzen, D. E. & Morshedi, D. (2025). The neuroprotective effect of human umbilical cord MSCs-derived secretome against α-synuclein aggregates on the blood-brain barrier. International Journal of Biological Macromolecules, 304(Part 1), Article 140387. https://doi.org/10.1016/j.ijbiomac.2025.140387

Paiva, P., Teixeira, L. M. C., Ferreira, P., Otzen, D. E., Fernandes, P. A. & Ramos, M. J. (2025). Transforming Computational Power into Environmental Solutions: HPC-driven Research on Urethanase-mediated Plastic Degradation. Procedia Computer Science, 267, 207-217. https://doi.org/10.1016/j.procs.2025.08.247

Paiva, P., Teixeira, L. M. C., Wei, R., Liu, W., Weber, G., Morth, J. P., Westh, P., Petersen, A. R., Johansen, M. B., Sommerfeldt, A., Sandahl, A., Otzen, D. E., Fernandes, P. A. & Ramos, M. J. (2025). Unveiling the enzymatic pathway of UMG-SP2 urethanase: insights into polyurethane degradation at the atomic level. Chemical Science, 16(5), 2437-2452. https://doi.org/10.1039/d4sc06688j

Shiraz, M. G., Nielsen, J., Widmann, J., Chung, K. H. K., Davis, T. P., Rasmussen, C., Scavenius, C., Enghild, J. J., Martin-Gallausiaux, C., Singh, Y., Javed, I. & Otzen, D. E. (2025). Young rat microbiota extracts strongly inhibit fibrillation of α-synuclein and protect neuroblastoma cells and zebrafish against α-synuclein toxicity. Molecules and Cells, 48(1), Article 100161. https://doi.org/10.1016/j.mocell.2024.100161

Otzen, D. E. (2024). Antibodies and α-synuclein: What to target against Parkinson's Disease? Biochimica et Biophysica Acta - Proteins and Proteomics, 1872(2), Article 140943. https://doi.org/10.1016/j.bbapap.2023.140943

Santos, J., Cuellar, J., Pallarès, I., Byrd, E. J., Lends, A., Moro, F., Abdul-Shukkoor, M. B., Pujols, J., Velasco-Carneros, L., Sobott, F., Otzen, D. E., Calabrese, A. N., Muga, A., Pedersen, J. S., Loquet, A., Valpuesta, J. M., Radford, S. E. & Ventura, S. (2024). A Targetable N-Terminal Motif Orchestrates α-Synuclein Oligomer-to-Fibril Conversion. Journal of the American Chemical Society, 146(18), 12702-12711. https://doi.org/10.1021/jacs.4c02262

Otzen, D. & d'Angelo, J. (2024). BBA: 2024 and beyond. Biochimica et Biophysica Acta - General Subjects, 1868(2), Article 130524. https://doi.org/10.1016/j.bbagen.2023.130524

Pirhaghi, M., Mamashli, F., Moosavi-Movahedi, F., Arghavani, P., Amiri, A., Davaeil, B., Mohammad-Zaheri, M., Mousavi-Jarrahi, Z., Sharma, D., Langel, Ü., Otzen, D. E. & Saboury, A. A. (2024). Cell-Penetrating Peptides: Promising Therapeutics and Drug-Delivery Systems for Neurodegenerative Diseases. Molecular Pharmaceutics, 21(5), 2097-2117. https://doi.org/10.1021/acs.molpharmaceut.3c01167

Olsen, W. P., Courtade, G., Peña-Díaz, S., Nagaraj, M., Sønderby, T. V., Mulder, F. A. A., Malle, M. G. & Otzen, D. E. (2024). CsgA gatekeeper residues control nucleation but not stability of functional amyloid. Protein Science, 33(10), Article e5178. https://doi.org/10.1002/pro.5178

Otzen, D. E. (2024). Editorial: Research methods in biochemistry and biophysics. BBA advances, 6, Article 100121. https://doi.org/10.1016/j.bbadva.2024.100121

Serpell, L. C., Otzen, D. & Radford, S. E. (2024). Expanding the Protein Universe. Journal of Molecular Biology, 436(21), 168812. Article 168812. https://doi.org/10.1016/j.jmb.2024.168812

Minero, G. A. S., Møllebjerg, A., Thiesen, C., Johansen, M. I., Jørgensen, N. P., Birkedal, V., Otzen, D. E. & Meyer, R. L. (2024). Extracellular G-quadruplexes and Z-DNA protect biofilms from DNase I, and G-quadruplexes form a DNAzyme with peroxidase activity. Nucleic Acids Research, 52(4), 1575-1590. https://doi.org/10.1093/nar/gkae034

Peña-Díaz, S., Olsen, W. P., Wang, H. & Otzen, D. E. (2024). Functional Amyloids: The Biomaterials of Tomorrow? Advanced Materials, 36(18), Article 2312823. https://doi.org/10.1002/adma.202312823

Dilamian, M., Montazer, M., Yousefi, H., Otzen, D. E. & Morshedi, D. (2024). Functional porous protein nanofibrils/polysaccharides aerogel beads for efficient dyes removal from water. Materials Advances, 5(18), 7199-7221. https://doi.org/10.1039/d4ma00380b

Nowak, J. S. & Otzen, D. E. (2024). Helping proteins come in from the cold: 5 burning questions about cold-active enzymes. BBA advances, 5, Article 100104. https://doi.org/10.1016/j.bbadva.2023.100104

Otzen, D. E., Møllebjerg, A., Hinge, M. & Pathak, G. S. (2024). Hvordan opnår vi en bæredygtig plastikkultur? Aktuel Naturvidenskab, 2024(1), 15-18.

Knudsen, L. J., Nielsen, S. D.-H., Dekker, P., Otzen, D. E., Rauh, V. & Larsen, L. B. (2024). Impact of hydrolysis method and lactase preparation on proteolysis and glycation in long-term stored lactose-hydrolysed UHT milk. International Dairy Journal, 154, Article 105946. https://doi.org/10.1016/j.idairyj.2024.105946

Knudsen, L. J., Rauh, V., Pedersen, J. N., Dekker, P., Otzen, D. E., Larsen, L. B. & Nielsen, S. D. H. (2024). Insight into protein crosslinking and casein polymerization in pre- and posthydrolyzed lactose-free ultra-high-temperature milk during long-term storage. Journal of Dairy Science, 107(12), 10497-10511. https://doi.org/10.3168/jds.2024-25103

Shahsavari, S., Rad, M. B., Hajiaghajani, A., Rostami, M., Hakimian, F., Jafarzadeh, S., Hasany, M., Collingwood, J. F., Aliakbari, F., Fouladiha, H., Bardania, H., Otzen, D. E. & Morshedi, D. (2024). Magnetoresponsive liposomes applications in nanomedicine: A comprehensive review. Biomedicine & Pharmacotherapy, 181, Article 117665. https://doi.org/10.1016/j.biopha.2024.117665

Peña-Díaz, S., Ferreira, P., Ramos, M. J. & Otzen, D. E. (2024). Mining and engineering activity in catalytic amyloids. Methods in Enzymology, 697, 345-422. https://doi.org/10.1016/bs.mie.2024.03.002

Nielsen, J., Lauritsen, J., Pedersen, J. N., Nowak, J. S., Bendtsen, M. K., Kleijwegt, G., Lusser, K., Pitarch, L. C., Moreno, J. V., Schneider, M. M., Krainer, G., Goksøyr, L., Khalifé, P., Kaalund, S. S., Aznar, S., Kjærgaard, M., Sereikaité, V., Strømgaard, K., Knowles, T. P. J. ... Otzen, D. E. (2024). Molecular properties and diagnostic potential of monoclonal antibodies targeting cytotoxic α-synuclein oligomers. npj Parkinson's Disease , 10(1), Article 139. https://doi.org/10.1038/s41531-024-00747-6

Adam, L., Kumar, R., Arroyo-Garcia, L. E., Molenkamp, W. H., Nowak, J. S., Klute, H., Farzadfard, A., Alkenayeh, R., Nielsen, J., Biverstål, H., Otzen, D. E., Johansson, J. & Abelein, A. (2024). Specific inhibition of α-synuclein oligomer generation and toxicity by the chaperone domain Bri2 BRICHOS. Protein Science, 33(8), Article e5091. https://doi.org/10.1002/pro.5091

Žvirblis, M., Sakalauskas, A., Janvand, S. H. A., Dudutienė, V., Žiaunys, M., Sniečkutė, R., Otzen, D. E., Smirnovas, V. & Matulis, D. (2024). Structure-Activity Relationship of Fluorinated Benzenesulfonamides as Inhibitors of Amyloid-β Aggregation. Chemistry - A European Journal, 30(58), Article e202402330. https://doi.org/10.1002/chem.202402330

Displaying results 1 to 50 out of 469

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen