Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Andersen, MS , Levin, G & Odgaard, MV 2019, 'Economic benefits of reducing agricultural N losses to coastal waters for seaside recreation and real estate value in Denmark', Marine Pollution Bulletin, vol. 140, pp. 146-156. https://doi.org/10.1016/j.marpolbul.2019.01.010

Djuve, VL & Knutsen, CH 2019 'Economic Crisis and Regime Transitions From Within' V-Dem Institute, Department of Political Science, University of Gothenburg, Gothenburg. <https://www.v-dem.net/media/filer_public/2b/65/2b65b593-0029-4dc7-bc84-685b52baac41/wp_92_final.pdf>

Andersen, DDE & Krishnarajan, S 2019, 'Economic Crisis, Bureaucratic Quality, and Democratic Breakdown', Government and Opposition, vol. 54, no. 4, pp. 715-744. https://doi.org/10.1017/gov.2017.37

Krishnarajan, S 2019, 'Economic crisis, natural resources, and irregular leader removal in autocracies', International Studies Quarterly, vol. 63, no. 3, pp. 726-741. https://doi.org/10.1093/isq/sqz006

Knutsen, CH, Gerring, J, Skaaning, S-E, Teorell, J, Maguire, M, Coppedge, M & Lindberg, S 2019, 'Economic Development and Democracy: An Electoral Connection', European Journal of Political Research, vol. 58, no. 1, pp. 292-314. https://doi.org/10.1111/1475-6765.12282

Nianogo, RA, Wang, MC, Basurto-Davila, R, Nobari, TZ, Prelip, M, Arah, OA & Whaley, SE 2019, 'Economic evaluation of California prenatal participation in the Special Supplemental Nutrition Program for Women, Infants and Children (WIC) to prevent preterm birth', Preventive Medicine, vol. 124, pp. 42-49. https://doi.org/10.1016/j.ypmed.2019.04.011

Aarup Duus, L, Gulden, T, Che, C & Graumann, O 2019, 'Economic evaluation of percutaneous cryoablation versus partial nephrectomy of small renal masses. A systematic review', Nordic Congress of Radiology, København, Denmark, 22/05/2019 - 24/05/2019.

Williams, JH, Wezel, A & Ranaldo, M 2019, EcoStack scoping study and review of the current system state: Stacking of ecosystem services, mechanisms and interactions for optimal crop protection, pollination enhancement, and productivity. EU Commission. <https://ec.europa.eu/research/participants/documents/downloadPublic/YWJqTUkxN0R6TktuMjdmVGFhVEkzQ3NzRmpTQmllcG9LYitMMUZOR1NTeDBMTmtkcW1kMG5RPT0=/attachment/VFEyQTQ4M3ptUWNXSEFsRGhPWXYvanFhR2JYeVpqZys=>

Voigt, C, Marushchak, ME, Mastepanov, M, Lamprecht, RE, Christensen, TR, Dorodnikov, M, Jackowicz-Korczyński, M, Lindgren, A, Lohila, A, Nykänen, H, Oinonen, M, Oksanen, T, Palonen, V, Treat, CC, Martikainen, PJ & Biasi, C 2019, 'Ecosystem carbon response of an Arctic peatland to simulated permafrost thaw', Global Change Biology, vol. 25, no. 5, pp. 1746-1764. https://doi.org/10.1111/gcb.14574

Maar, M 2019, 'Ecosystem modelling of environmental effects from mussel farms', Coastal & Marine, vol. 28, no. 1, pp. 12-13. <https://www.eucc.net/uploads/12/coastalandmarine_2019-1.pdf>

Schmidt, NM , Hansen, J , Hansen, LH & Raundrup, KR 2019, Ecosystem-wide reproductive failure. in TR Christensen, MF Arndal & E Topp-Jørgensen (eds), Greenland Ecosystem Monitoring: Annual Report Cards 2018. Aarhus University, pp. 6-7.

Kahan, K, Kar, S, Sanderson, H, Roy, K & Leszczynski, J 2019, 'Ecotoxicological modeling, ranking and prioritization of pharmaceuticals using QSTR and i-QSTTR approaches: Application of 2D and fragment based descriptors', Molecular Informatics, vol. 38, no. 8-9, 1800078. https://doi.org/10.1002/minf.201800078

Andersen, H, Mantegazza, R, Wang, JJ, O’Brien, F, Patra, K, Howard, JF & The REGAIN Study Group 2019, 'Eculizumab improves fatigue in refractory generalized myasthenia gravis', Quality of Life Research, vol. 28, no. 8, pp. 2247-2254. https://doi.org/10.1007/s11136-019-02148-2

Blömer, M 2019, Edessa and Osrhoene. in O Tekin (ed.), Hellenistic and Roman Anatolia. Kings, Emperors, City States. Phoibos Verlag, Istanbul, pp. 196-221.

Liu, J & Zhang, Q 2019, Edge Computing Enabled Mobile Augmented Reality with Imperfect Channel Knowledge. in European Wireless 2019 Conference, EW 2019. VDE Verlag GmbH, pp. 90-95, European Wireless 2019 - 25th European Wireless Conference, Aarhus, Denmark, 02/05/2019. <https://www.vde-verlag.de/proceedings-en/564948016.html>

Wang, Y , Wang, Z , Yang, Q, Hua, A, Ma, S, Zhang, Z & Dong, MD 2019, 'Edge-oriented MoS2 supported on nickel/carbon core–shell nanospheres for enhanced hydrogen evolution reaction performance', New Journal of Chemistry, vol. 43, no. 16, pp. 6146-6152. https://doi.org/10.1039/c9nj00612e, https://doi.org/10.1039/C9NJ00612E

Whitehead, C, Daugbjerg, M & Bozoğlu, G 2019, Edges and centres: the forcefields of European heritage. in C Whitehead, S Eckersley, M Daugbjerg & G Bozoğlu (eds), Dimensions of Heritage and Memory: Multiple Europes and the Politics of Crisis. Routledge, London, pp. 96-121.

Fissore, RA, Burton, A & Lykke-Hartmann, K 2019, 'Editorial: Molecular and Cellular Mechanisms in Reproduction and Early Development', Frontiers in Cell and Developmental Biology, vol. 7, no. MAR, 36, pp. 36. https://doi.org/10.3389/fcell.2019.00036

Pakkenberg, B, Olesen, MV, Kaalund, SS & Dorph-Petersen, K-A 2019, 'Editorial: Neurostereology', Frontiers in Neuroanatomy, vol. 13, 42. https://doi.org/10.3389/fnana.2019.00042

Valette, J, Ronen, I & Jespersen, SN 2019, 'Editorial: Assessing cellular microstructure in biological tissues using in vivo diffusion-weighted magnetic resonance', Frontiers in Physics, vol. 7, 37. https://doi.org/10.3389/fphy.2019.00037

Farrow, K, Isa, T, Luksch, H & Yonehara, K 2019, 'Editorial: The superior colliculus/tectum: Cell types, circuits, computations, behaviors', Frontiers in Neural Circuits, vol. 13, 39. https://doi.org/10.3389/fncir.2019.00039

Cedzyński, M, Thielens, NM, Mollnes, TE & Vorup-Jensen, T 2019, 'Editorial: The Role of Complement in Health and Disease', Frontiers in Immunology, vol. 10, 1869. https://doi.org/10.3389/fimmu.2019.01869

Bergqvist, E, Fauskanger, J, Häggström, J, Hähkiöiemi, M & Højgaard, T 2019, 'Editorial', Nordic Studies in Mathematics Education, vol. 24, no. 1, pp. 1-4. <http://ncm.gu.se/media/nomad/24_1_001004_editorial.pdf>

Bergqvist, E, Fauskanger, J, Häggström, J, Hähkiöiemi, M & Højgaard, T 2019, 'Editorial', Nordic Studies in Mathematics Education, vol. 24, no. 2, pp. 1-3. <http://ncm.gu.se/media/nomad/24_2_001004_editorial.pdf>

Aschner, M, Autrup, HN, Cohen, SM, Dekant, W, Eisenbrand, G, Galli, CL, Gori, GB, Kaminski, NE, Klaassen, CD, Klaunig, JE, Levy, L, Lotti, M, Marquardt, HWJ, Pelkonen, O, Schrenk, DF & Yamazaki, H 2019, 'Editorial', Regulatory Toxicology and Pharmacology, vol. 101, no. February, pp. A1-A2. https://doi.org/10.1016/j.yrtph.2018.12.015

Foo, E, Plett, JM, Lopez-Raez, JA & Reid, D 2019, 'Editorial: The Role of Plant Hormones in Plant-Microbe Symbioses', Frontiers in Plant Science, vol. 10, 1391. https://doi.org/10.3389/fpls.2019.01391

Kristiansen, MS, Iversen, R, Grane, T & Sørensen, L 2019, 'Editorial', Danish Journal of Archaeology, vol. 8, pp. 1-2. https://doi.org/10.7146/dja.v8i0.115607

Lee, BP, Birkedal, H & Lee, H 2019, 'Editorial: Catechol and Polyphenol Chemistry for Smart Polymers', Frontiers in Chemistry, vol. 7, 883. https://doi.org/10.3389/fchem.2019.00883

Mookerjee, RP & Thomsen, KL 2019, 'Editorial: evaluating cirrhosis progression-lessons from the heart. Authors' reply', Alimentary Pharmacology and Therapeutics, vol. 50, no. 6, pp. 710-711. https://doi.org/10.1111/apt.15463

Nissen, P 2019, 'Editorial', Kognition & Pædagogik, vol. 29, no. 111 / 112, pp. 3 -5.

Bergqvist, E, Fauskanger, J, Häggström, J, Hähkiöiemi, M & Højgaard, T 2019, 'Editorial', Nordic Studies in Mathematics Education, vol. 24, no. 3-4, pp. 12.

Bennedsen, J, Edström, K, Kuptasthien, N, Lauritsen, AB, Roslöf, J & Songer, R 2019, 'Editorial', Proceedings of the International CDIO Conference.

Raffnsøe, S, Beaulieu, A, Feldman, A, Cruikshank, B, O'Farrell, C, Lorenzini, D, Taylor, D, McGushin, E, Petersen, EB, Mascaretti, G, Martinez, HCP, Oksala, J, Eliassen, KO, Lawlor, LR, Tazzioli, M, Orellana, RC, Götselius, T, Erlenbusch, V, Gudmand-Høyer, M, Rosenberg, A, Neumann, SH & Staunæs, AB 2019, 'Editorial', Foucault Studies, vol. 27, no. 27, pp. I-VI. https://doi.org/10.22439/FS.V27I27.5887

Kierkegaard, S 2019, 'Editorial Commentary: The Gain in Pain After Hip Arthroscopic Surgery: What Is Clinically Relevant, and Is Pain Related to Function in Patients With Femoroacetabular Impingement Syndrome?', Arthroscopy: The Journal of Arthroscopy & Related Surgery, vol. 35, no. 7, pp. 2070-2071. https://doi.org/10.1016/j.arthro.2019.03.014

Andersen, CU & Cox, G 2019, 'Editorial - Feeling, Failure, Fallacies', A Peer-Reviewed Journal About, vol. 8, no. 1, pp. 5-7. <https://aprja.net//article/download/115409/163720/>

Jørgensen, D 2019, 'Editorial Introduction for the Topical Issue “Experience in a New Key”', Open Philosophy, vol. 2, no. 1, pp. 700-705. https://doi.org/10.1515/opphil-2019-0052

Deleuran, M & Ständer, S 2019, 'Editorial overview: dermatology is moving forward', Current Opinion in Pharmacology, vol. 46, no. June, pp. vi-viii. https://doi.org/10.1016/j.coph.2019.06.002

Ferapontova, EE & Fojta, M 2019, 'Editorial Overview: Bioelectrochemistry Electrochemistry of biopolymers and electrochemical biosensors Dedicated to the Memory of Professor Emil Palecek (*Oct 03, 1930 - dagger Oct 30, 2018)', Current Opinion in Electrochemistry, vol. 14, pp. A1-A3. https://doi.org/10.1016/j.coelec.2019.04.017

Andersen, JH, Carstensen, J, Holmer, M, Krause-Jensen, D & Richardson, K 2019, 'Editorial: Research and management of eutrophication in coastal ecosystems', Frontiers in Marine Science, vol. 6, 768. https://doi.org/10.3389/fmars.2019.00768

Karlsson, S, Andell, P, Mohammad, MA, Koul, S, Olivecrona, GK, James, SK, Fröbert, O & Erlinge, D 2019, 'Editor's Choice- Heparin pre-treatment in patients with ST-segment elevation myocardial infarction and the risk of intracoronary thrombus and total vessel occlusion. Insights from the TASTE trial', European heart journal. Acute cardiovascular care, vol. 8, no. 1, pp. 15-23. https://doi.org/10.1177/2048872617727723

Clough, RE, Barillà, D, Delsart, P, Ledieu, G, Spear, R, Crichton, S, Mounier Vehier, C, Peacock, J, Sobocinski, J & Haulon, S 2019, 'Editor's Choice – Long-term Survival and Risk Analysis in 136 Consecutive Patients With Type B Aortic Dissection Presenting to a Single Centre Over an 11 Year Period', European Journal of Vascular and Endovascular Surgery, vol. 57, no. 5, pp. 633-638. https://doi.org/10.1016/j.ejvs.2018.08.042

Behrendt, C-A, Björck, M, Schwaneberg, T, Debus, ES, Cronenwett, J, Sigvant, B & Acute Limb Ischaemia Collaborators 2019, 'Editor's Choice - Recommendations for Registry Data Collection for Revascularisations of Acute Limb Ischaemia: A Delphi Consensus from the International Consortium of Vascular Registries', European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery, vol. 57, no. 6, pp. 816-821. https://doi.org/10.1016/j.ejvs.2019.02.023

Winding, A , Bang-Andreasen, T , Hansen, LH , Panitz, F , Krogh, PH , Krause-Jensen, D , Stæhr, P , Nicolaisen, M , Hendriksen, NB , Sapkota, R , Santos, S & Andersen, LW 2019, eDNA in environmental monitoring. Teknisk rapport fra DCE - Nationalt Center for Miljø og Energi, no. 133, Aarhus University, DCE - Danish Centre for Environment and Energy, Aarhus. <https://dce2.au.dk/pub/TR133.pdf>

Sparre, SL 2019, Educated hope in Cairo: The formation of Young Muslim Citizens. in JN Dorio, ED Abdou & N Moheyeldine (eds), The Struggle for Citizenship Education in Egypt: (Re)Imagining Subjects and Citizens . Routledge, New York, pp. 77-90.

Jensen, NR 2019, 'Educating children and young people in care: learning placements and caring schools', European Journal of Social Work, vol. 22, no. 6, pp. 1098-1099. https://doi.org/10.1080/13691457.2019.1607021

Jakobsen, M , Mitchell, F , Nørreklit, H & Trenca, M 2019, 'Educating management accountants as business partners: Pragmatic constructivism as an alternative pedagogical paradigm for teaching management accounting at master’s level', Qualitative Research in Accounting and Management, vol. 16, no. 4, pp. 517-541. https://doi.org/10.1108/QRAM-10-2017-0099

Kanareva-Dimitrovska, A 2019, 'Educating the Global Citizen through telecollaboration', Educating the Global Citizen: International Perspectives on Foreign Language Teaching in the Digital Age, Munich, Germany, 25/03/2019 - 28/03/2019.

Morell Hjortshøj, N 2019, 'Educating the Innovative and Entrepreneurial Individual in China', 3E Conference - ECSB Entrepreneurship Education Conference, 2019, Göteborg, Sweden, 07/05/2019 - 10/05/2019.

Doser, K, Kenborg, L, Andersen, EW, Bidstrup, PE, Kroyer, A, Hove, H, Østergaard, J, Sørensen, SA, Johansen, C, Mulvihill, J, Winther, JF & Dalton, SO 2019, 'Educational delay and attainment in persons with neurofibromatosis 1 in Denmark', European Journal of Human Genetics, vol. 27, no. 6, pp. 857-868. https://doi.org/10.1038/s41431-019-0359-8

Poulsen, M , Friesgaard, KD , Seidenfaden, S , Paltved, C & Nikolajsen, L 2019, 'Educational interventions to improve medical students' knowledge of acute pain management: a randomized study', Scandinavian Journal of Pain, vol. 19, no. 3, pp. 619-622. https://doi.org/10.1515/sjpain-2019-0036

Displaying results 96401 to 96450 out of 389685

1929