Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Almeida da Paz, M, Yildiz, U, Kim, M, Campos-Fornés, V, Pinkasz, M, Dahlet, T, Noh, KM & Taher, L 2026, 'MER57E3 transposable elements regulate gene expression in a human cell model of neural development', Genome Biology, vol. 27, no. 1, 66. https://doi.org/10.1186/s13059-026-03946-x

Ofosu, GK, Seeberg, MB & Wahman, M 2026, 'Messages matter: How voter education campaigns affect citizens’ willingness to vote for women', The Journal of Politics, vol. 88, no. 1, pp. 145-161. https://doi.org/10.1086/734252

Meng, Y, Liu, X, Li, Y, Huang, L, Tian, X, Ata-Ul-Karim, ST, Yu, K, Zha, H, Liu, X, Tian, Y, Zhu, Y, Cao, W & Cao, Q 2026, 'Meta-analysis of environmental-induced changes in optimal nitrogen rate of wheat at regional scale in China', Field Crops Research, vol. 338, 110295. https://doi.org/10.1016/j.fcr.2025.110295

Dittrich, A , Andersson, SA , Busk, M , Hansen, K , Foldager, CB , Palmfeldt, J , Andersen, A , Pedersen, M , Vendelbo, M , Nielsen, KL & Lauridsen, H 2026, 'Metabolic changes during cardiac regeneration in the axolotl', Developmental Dynamics, vol. 255, no. 1, pp. 66-85. https://doi.org/10.1002/dvdy.70020

Baur, T, Allaart, MT, Zipperle, A, Contreras, G, Fink, C, Angenent, LT & Molitor, B 2026, 'Metabolic engineering of Methanothermobacter thermautotrophicus ΔH for recombinant acetoin production', Metabolic Engineering Communications, vol. 22, e00275. https://doi.org/10.1016/j.mec.2026.e00275

Kjærgaard, U 2026, 'Metabolic Imaging of Hepatocellular Carcinoma with Hyperpolarized MRI', PhD, Aarhus University.

Naik, AS, Alakwaa, FM, Nair, V, McCown, PJ, Schaub, JA, Otto, EA, Menon, R, Annese, F, Choi, YJ, Hampson, HE, Inge, TH, Hartman, J, Eddy, S, Smith, C, Hodgin, JB, Inoki, K, Srivastava, SP, Al-Fagih, K, Yoshida, S, Goodrich, JA, Cree, MG, Narongkiatikhun, P, Yuan, L, Tommerdahl, KL, Prasad, P, van Raalte, DH, Kelsey, MM, Ryder, JR, Dobbs, TJ, Ladd, P, Pennathur, S, Nelson, RG, Okabayashi, Y, Puelles, VG, Ferrence-Salo, J, Beamish, JA, Brosius, FC, Nadeau, KJ, Pyle, L, Kretzler, M & Bjornstad, P 2026, 'Metabolic surgery mitigates early kidney injury in obese youth with diabetes by suppressing mTORC1/JAK/STAT signaling', The Journal of clinical investigation, vol. 136, no. 7. https://doi.org/10.1172/JCI198545

Mikkelsen, ACD , Amby, KK, Lauridsen, MM & Thomsen, KL 2026, 'Metabolisk dysfunktionsassocieret steatotisk leversygdom kan påvirke hjernen', Ugeskrift for Læger, vol. 188, no. 7. https://doi.org/10.61409/V07250544

R⊘nn, M , N⊘rskov, NP , Thorsteinsson, M & Nielsen, MO 2026, 'Metabolism of iodoform in dairy cows: Absorption and excretory pathways', Journal of Dairy Science, vol. 109, no. 4, pp. 3637-3649. https://doi.org/10.3168/jds.2025-27329

Li, X, Wang, Y, Bahram, M, Xian, Y, Huang, R, Tedersoo, L, Wang, C & Li, B 2026, 'Metabolomic responses of free-living diazotrophs inoculated on biochar', Journal of Environmental Chemical Engineering, vol. 14, no. 1, 121068. https://doi.org/10.1016/j.jece.2026.121068

Mazancieux, A, Hat, K, Rutiku, R, Wierzchoń, M & Sandberg, K 2026, 'Metacognition in Decision-Making Across Domains and Modalities: Evidence From Three Studies', Psychological Science, vol. 37, no. 2, pp. 106-124. https://doi.org/10.1177/09567976251415354

Laurent, M , Baysinger, MR, Bartholomäus, A, Windirsch, T, Strauss, J, Sanders, T, Liebner, S & Treat, C 2026, 'Metagenomic Analysis of Thawing Permafrost Highlights Links Between Carbon and Nitrogen Cycling Processes in Abrupt Thaw Simulation', Journal of Geophysical Research: Biogeosciences, vol. 131, no. 3, e2025JG009630. https://doi.org/10.1029/2025JG009630

Bellankimath, AB, Branders, S, Kegel, I, Ali, J, Asadi, F, Johansen, TEB, Imirzalioglu, C, Hain, T, Wagenlehner, F & Ahmad, R 2026, 'Metagenomic sequencing enables accurate pathogen and antimicrobial susceptibility profiling in complicated UTIs in approximately four hours', Nature Communications, vol. 17, no. 1, 187. https://doi.org/10.1038/s41467-025-66865-8

Lin, Q , Cai, X, Zhong, Z, Li, T, Chen, X, Ayalew, W, Xu, Z, Wei, C, Zhang, X, Cheng, H, Zhang, Z, Li, X, Tang, Y, Chen, S, Zhou, J, Si, J, Wu, X, Ning, C, Wang, Q, Pan, Y, Gao, Y, Li, J, Yu, Y, Zhang, Z, Zhao, Y, Fang, L & Zhang, Z 2026, 'Meta-GWAS of Pig Semen Quality Traits Reveals Conserved Genes Regulating Mammalian Fertility', Advanced Science, vol. 13, no. 16, e15203. https://doi.org/10.1002/advs.202515203

Kristensen, LR 2026, 'Metal hydridoborate derivatives as fast solid-state ion conductors', PhD, Aarhus University.

Wang, F , Vig Benfeldt, F , Khazaee, Z , Nygaard Nielsen, ML , Marks, MJ , Ouyang, Y , Lock, N, Gao, R, Guo, Z & Eser, BE 2026, 'Metal–Organic Framework Encapsulation of Ferulic Acid Decarboxylase: Robust Biocomposites Catalyzing Synthetically Useful Decarboxylation and Synergized CO₂Fixation Reactions', ACS Sustainable Chemistry and Engineering, vol. 14, no. 4, pp. 1884-1898. https://doi.org/10.1021/acssuschemeng.5c09355

Liebermann, R 2026, Metaphors of Judgement. in Handbook on the Book of Ezekiel. Equinox Publishing.

Pallesen, LT, Pedersen, A-K, Sankaranarayanan, I, Feldman-Goriachnik, R, Ahlgreen, OA , Pinheiro, AM, He, L, Baake, J , Hansen, MW , Richner, M , Ellegaard, LD, Petersen, KA, Fjord-Larsen, L, Olsen, JV, Munro, G, Hanani, M, Price, TJ & Vægter, CB 2026, 'Meteorin Resolves Nociceptive Hypersensitivity by Reducing Connexin-Mediated Coupling in Satellite Glial Cells', Glia, vol. 74, no. 2, e70105. https://doi.org/10.1002/glia.70105

Lynderup, EM , Vendelbo, M , Kirk, FT , Vase, KH , Alstrup, AKO, Rieder, T, DiSpirito, AA, Semrau, JD, Laursen, TL , Ott, P, Zischka, H & Sandahl, TD 2026, 'Methanobactin rapidly facilitates biliary copper excretion in a Wilson disease rat model visualised by ⁶⁴Cu PET/MRI', British Journal of Pharmacology, vol. 183, no. 2, pp. 268-279. https://doi.org/10.1111/bph.70099

Rivas-Arrieta, MJ , Zoppi, G , Carlslund Høst, NK & Biller, P 2026, 'Methanol-Assisted Hydrothermal Liquefaction of Manure–Straw Mixtures: Reactive Role and Solvent Fate', Energy and Fuels, vol. 40, no. 14, pp. 7510-7522. https://doi.org/10.1021/acs.energyfuels.6c00004

Krzyslak, H, Szejniuk, WM, Falkmer, U, Honoré, B, Jørgensen, MM, Sten, C, Pedersen, S, Christiansen, G & Kristensen, SR 2026, 'Methodological and Short-Term Diurnal Variation in Surface and Cargo Proteins in Plasma Extracellular Vesicles', Current issues in molecular biology, vol. 48, no. 1, 120. https://doi.org/10.3390/cimb48010120

Duin, JJ, Smits, RAL, Krans, T, Dekkers, OM, Trompet, S, de Groot, B, Mooijaart, SP & van den Bos, F 2026, 'Methodological approaches to estimate physical resilience in older adults: a comparison across two clinical settings', Age and Ageing, vol. 55, no. 2, afag025. https://doi.org/10.1093/ageing/afag025

Pedersen, J , Hafner, SD & Pacholski, A 2026, 'Methodological factors affecting ammonia emission measurement with flux chambers from field-applied biogas digestate slurry (Technical note)', Atmospheric Environment: X, vol. 29, 100408. https://doi.org/10.1016/j.aeaoa.2025.100408

Evenhuis, A, Bain, RA, Abuley, IK, Hausladen, H & Schepers, HTAM 2026, 'Methodology to Determine Fungicide Efficacy Ratings for the EuroBlight Tables – Potato Late or Early Blight', Potato Research, vol. 69, no. 3, 68. https://doi.org/10.1007/s11540-026-10014-1

Matousek, C 2026 'Metric completions of triangulated categories from finite dimensional algebras'. https://doi.org/10.48550/arXiv.2409.01828

Matousek, C 2026 'Metric completions of triangulated categories from hereditary rings'. https://doi.org/10.48550/arXiv.2508.20283

Rodriguez-Palomo, A , Didziokas, M , Jacobsen, MSB, Christensen, TEK, Kantor, I, Jørgensen, MRV, Almtoft, KP, Thomsen, JS, Herrel, A, Williams, C & Birkedal, H 2026, 'Micro and Nanostructural Diversity of Lizard Osteoderm Capping Tissue in Relation to Mechanical Performance', Advanced Functional Materials, vol. 36, no. 26, e26169. https://doi.org/10.1002/adfm.202526169

Sakai, Y, Behrends, C, Cuervo, AM, Debnath, J, Izumi, M, Jenny, A, Molinari, M, Nakamura, S, Oku, M, Otegui, MS, Santambrogio, L, Shen, H-M, Taguchi, T, Thumm, M, Ushimaru, T, Xie, Z & Reggiori, F 2026, 'Microautophagy: definition, classification, and the complexity of the underlying mechanisms', Autophagy, vol. 22, no. 1, pp. 3-9. https://doi.org/10.1080/15548627.2025.2559687

Sakai, Y, Behrends, C, Debnath, J, Izumi, M, Jenny, A, Molinari, M, Nakamura, S, Oku, M, Otegui, MS, Santambrogio, L, Shen, H-M, Taguchi, T, Thumm, M, Ushimaru, T, Xie, Z, Cuervo, AM & Reggiori, F 2026, 'Microautophagy: current understanding of its molecular mechanisms and functions', Autophagy Reports, vol. 5, no. 1, 2626661. https://doi.org/10.1080/27694127.2026.2626661

DanGer Shock investigators 2026, 'Microaxial Flow Pump Use in Different Phenotypes of Cardiogenic Shock - a Secondary Analysis of the DanGer Shock Trial', Journal of Cardiac Failure. https://doi.org/10.1016/j.cardfail.2026.02.015

Xu, J, Verma, E, Sanchez, J, Gharaie, S, Jeong, S, Patel, SK, Gooya, M, Rosa, XDL, Gupta, K, Rinschen, MM, Rabb, H & Pluznick, JL 2026, 'Microbes and Glomerular Filtration Rate in Health and CKD in Mice', Journal of the American Society of Nephrology, vol. 37, no. 1, pp. 36-48. https://doi.org/10.1681/ASN.0000000814

Shang, Y , Liang, Z , Siddique, IA, Dippold, M, Abalos, D & Olesen, JE 2026, 'Microbial and plant-derived carbon contributions to particulate and mineral-associated organic carbon in perennial and annual cropping systems', Geoderma, vol. 466, 117688. https://doi.org/10.1016/j.geoderma.2026.117688

Kindtler, NL, Sheikh, S, He, R, Fonseca, RRD, Laursen, KH & Ekelund, F 2026, 'Microbial diversity loss affects old and modern barley cultivars differently under varying nitrogen sources', Microbiological Research, vol. 306, 128458. https://doi.org/10.1016/j.micres.2026.128458

Zhang, W, Lu, Y, Peixoto, L, Ji, C, Yang, H, Wang, K, Zhang, J, Wang, J, Shi, L, Zhou, J, Li, FM & Sánchez-Rodríguez, AR 2026, 'Microbial efficiency drives depth-dependent soil carbon storage under organic fertilization', Journal of Environmental Management, vol. 402, 129010. https://doi.org/10.1016/j.jenvman.2026.129010

Kjeldsen, PL , Madsen, LS , Parbo, P , Ismail, R , Aanerud, JFA , Thomsen, MK , Damholdt, MF , Eskildsen, SF , Østergaard, L & Brooks, DJ 2026, 'Microglial activation is raised in preclinical Alzheimer’s disease and associated with covert memory impairment', Frontiers in dementia, vol. 4, 1745571. https://doi.org/10.3389/frdem.2025.1745571

Cavaletti, F & Heimann, K 2026, 'Micro-Phenomenology as a Method for Informing Experimental Design: A Case Study Regarding Time Perception', Constructivist Foundations, vol. 21, no. 2, pp. 137-149.

Rea, LT, Adamchak, C, Gerson, JR, Franchois, MA, Røy, H & Hinckley, ELS 2026, 'Microsites support microbial sulfate reduction in upland soils', Soil Biology and Biochemistry, vol. 216, 110121. https://doi.org/10.1016/j.soilbio.2026.110121

Knudsen, NM , Thrane, L , Emmanuel, T , Johansen, C , Pedersen, M & Vestergaard, C 2026, 'Microvascular alterations in nonlesional psoriatic skin versus healthy control skin: insights from optical coherence tomography angiography in psoriasis and atopic dermatitis', Skin Health and Disease, vol. 6, no. 1, pp. 45-53. https://doi.org/10.1093/skinhd/vzaf083

Nijkamp, T, Namba, HF, Boerhout, CKM, Lee, JM, de Waard, GA, Mejía-Rentería, H, Hoshino, M, Echavarria-Pinto, M, Meuwissen, M, Matsuo, H, Madera-Cambero, M, Eftekhari, A, Effat, MA, Banerjee, R, Murai, T, Marques, K, Doh, J-H, Jung, J-H, Nam, C-W, Niccoli, G, Nakayama, M, Tanaka, N, Shin, E-S, van Es, R, den Ruijter, HM, van der Harst, P, Knaapen, P, Koo, B-K, Kakuta, T, Escaned, J, van Royen, N, Christiansen, EH, Piek, JJ, Damman, P & van de Hoef, TP 2026, 'Microvascular Resistance Reserve vs Coronary Flow Reserve to Assess Age-Related Trends in Coronary Microvascular Dysfunction', JACC. Cardiovascular interventions, vol. 19, no. 4, pp. 437-448. https://doi.org/10.1016/j.jcin.2025.11.019

Rytter, M 2026, 'Midlertidighed, afmagt og flugt gennem skriften', Jordens Folk.

Rytter, M 2026, 'Midlertidighedens konsekvenser: Flygtninges liv og rettigheder', Samfundsfagsnyt.

Vergin, L , Madsen, J , Fox, AD , Therkildsen, OR & Clausen, KK 2026, 'Migratory geese adjust wintering movements to both short-term weather and long-term climatic change', Scientific Reports, vol. 16, no. 1, 10014. https://doi.org/10.1038/s41598-026-41003-6

Hammershøj, LG & Holsting, VS 2026, Militær professionsdannelse. Samfundslitteratur.

Ehlers, B & Damgaard, C 2026, 'Miljømæssig risikovurdering af ansøgning om udsætning af cisgene stivelseskartofler med flere komplementære resistensgener med øget resistens imod kartoffelskimmel (Phytophthora infestans)', No. 2026-0946614, 6 p., Apr 17, 2026. Rådgivningsnotat fra DCA - Nationalt Center for Fødevarer og Jordbrug.

Ehlers, B & Damgaard, C 2026, 'Miljømæssig risikovurdering af forsøgsudsætning af NGT-kartoffel', No. 2026-0933486, 7 p., Mar 09, 2026. Rådgivningsnotat fra DCA - Nationalt Center for Fødevarer og Jordbrug.

Damgaard, C & Ehlers, B 2026, 'Miljømæssig risikovurdering af genomredigeret byg i sædskifteforsøg', No. 2026-0933486, 6 p., Apr 17, 2026. Rådgivningsnotat fra DCA - Nationalt Center for Fødevarer og Jordbrug.

Adamsen, APS , Nørremark, M , Hansen, MJ , Kai, P , Børsting, CF , Brask, MJ , Ottosen, C-O & Kristensen, EF 2026, Miljø- og Klimateknologi 2026. Rådgivningsrapport fra DCA - Nationalt Center for Fødevarer og Jordbrug, Aarhus University, DCE - Danish Centre for Environment and Energy.

Ehlers, B & Bruus, M 2026, 'Miljørisikovurdering af ansøgning om brug af cisgene kartofler i sædskifteforsøg', No. 2026-0933486, 6 p., Apr 24, 2024. Rådgivningsnotat fra DCA - Nationalt Center for Fødevarer og Jordbrug.

Bohr, EH, Christensen, JPA , Tairova, Z & Timmermann, K (eds) 2026, Miljøtilstand og presfaktorer i Roskilde Fjord og Isefjorden. DTU Aqua-rapport, no. 492-2026, DTU Aqua. https://doi.org/10.11581/e3fcaaeb-7b5c-477d-bbe4-352809f7b6b1

Displaying results 2601 to 2650 out of 390508

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen