Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Matthews, JA, Linge, H, Wilson, P, Mourne, RW, Snook, P, Hill, JL & Olsen, J 2026, 'A comprehensive exposure-age dating approach to interpreting complex glacial and periglacial landscapes: The landform mosaic of Alnesdalen, a Norwegian alpine drainage basin', Holocene. https://doi.org/10.1177/09596836251414044

Larsen, NY , Paulsen, LB , Ahrends, C, Winkler, AM & Vidaurre, D 2026, 'A comprehensive framework for statistical testing of brain dynamics', Nature Protocols. https://doi.org/10.1038/s41596-025-01300-2

Nielsen, MK & Mikkelsen, J 2026, 'A computational study for batch-Level diagnostics in industrial extrusion using interpretable machine learning and counterfactual analysis', Journal of Food Engineering, vol. 413, 112999. https://doi.org/10.1016/j.jfoodeng.2026.112999

Dallasega, P, Kaushal, I, Revolti, A, Tripathi, A & Ma, J 2026, A Conceptual Assessment of Digital Twin Potential Through Discrete Event Simulation for Smart Mobile Factories in Linear Infrastructure Construction Projects. in H Mizuyama, E Morinaga, T Nonaka, T Kaihara, G von Cieminski & D Romero (eds), Advances in Production Management Systems. Cyber-Physical-Human Production Systems: Human-AI Collaboration and Beyond. Springer Nature, Cham, pp. 319-333. https://doi.org/10.1007/978-3-032-03542-4_22

Fusaro, M, Chandran, M, Nickolas, T, Ferrari, S, Cossettini, A, Ebeling, PR, Mc Closkey, E, Harvey, N, Pierroz, DD, Cannata-Andia, J, Cheung, A, Guglielmi, G, Glüer, C, Foldes, J, Lems, W, Trombetti, A, Mithal, A, Napoli, N, Haarhaus, M, Ferraro, PM, Ketteler, M, Bellasi, A, Moyses, R, Evenepoel, P, Jørgensen, HS, Reginster, JY, Halbout, P, Bover, J, Gallieni, M, Salam, S, Eastell, R, Hansen, D, E, C & Brandi, ML 2026, 'A consensus statement on the management of vertebral fractures in CKD stages G4-G5D', Nephrology Dialysis Transplantation, vol. 41, no. 3, pp. 584-598. https://doi.org/10.1093/ndt/gfaf256

Jaeger, SR, Chheang, SL, Hedderley, DI, Roigard, CM & Harker, FR 2026, 'A consumer segmentation study for honey: Mānuka and other mono-floral honey with indigenous provenance', International Journal of Gastronomy and Food Science, vol. 43, 101387. https://doi.org/10.1016/j.ijgfs.2025.101387

Sarswat, N, Ambardekar, AV, Alexander, KM, Cuddy, SA, Stern, L, Poulsen, SH, Tschöpe, C, Sekijima, Y, Sheikh, FH, Griffin, JM, Judge, DP, Gillmore, J & Masri, A 2026, 'Acoramidis in transthyretin amyloid cardiomyopathy: expanding evidence from ATTRibute-CM', Future Cardiology, vol. 22, no. 2, pp. 119-133. https://doi.org/10.1080/14796678.2025.2610641

Fusaroli, R , Weed, E , Cox, CMM, Szabó, BI, Fein, D & Naigles, L 2026, 'Acoustic Accommodation to Caregivers Is Stronger in Autistic Children', INSAR 2026, Prague, Czech Republic, 22/04/2026 - 25/04/2026.

Vestergaard, MB 2026, 'A Credible Change: How Parties Use Election Promises to Counteract The Loss of Reputation When They Dilute Their Policy Positions', Political Communication. https://doi.org/10.1080/10584609.2025.2600004

Sovacool, BK, Griffiths, S, Herman, K & Iskandarova, M 2026, 'A critical meta-survey of the lifecycle greenhouse gas emissions of hydrogen energy systems', Energy Research and Social Science. https://doi.org/10.1016/j.erss.2025.104461

Bai, L, Onwumere, J, Ezugwu, CI , Saad, A, Huang, Z, Xie, Z & Wei, Z 2026, 'Activating PFAS for efficient photocatalytic defluorination using ultralow-dose Ti-doped bismuth coordination catalysts with unsaturated metal sites', Applied Catalysis B: Environment and Energy, vol. 384, 126230. https://doi.org/10.1016/j.apcatb.2025.126230

Jost, ME, Schweizer, M, Henning, P, Gorzelanny, C, Lehners, M, Ellinger, B, Boix-Campos, J, Kux, JM, Singh, S, Fachinger, A, Martinez Pomier, K, VanSchouwen, B, Billing, AM, Biedenweg, D, Schweizer, M, Siegel, S, Reimer, R, Brandt, M, Priesmeier, L, Fuchs, U, Pflaumenbaum, J, Nikolaev, VO, Newbury-Ecob, R, Wilsdon, A, Rybczynski, M, Gehle, P, Zelarayán, LC, Stafforst, T, Feil, R, Rinschen, MM, Pless, O, Eaton, P, Sáez, PJ, Otto, O, Melacini, G, Demal, TJ, Eschenhagen, T, Herberg, FW & Cuello, F 2026, 'Activating PRKG1 Variant Enhances Smooth Muscle Cell Deformability To Cause Aortopathy', JACC: Basic to translational science, vol. 11, no. 2, 101452. https://doi.org/10.1016/j.jacbts.2025.101452

Edholm, P, Wennberg, P, Ballin, M, Östgren, C-J, Daka, B, Berglind, D, Lenander, C, Ahlqvist, VH, Rådholm, K, Henriksson, P & Herraiz-Adillo, Á 2026, 'Active commuting and atherosclerosis in a population-based sample of middle-aged adults: the Swedish CArdioPulmonary bioImage study (SCAPIS)', British Journal of Sports Medicine, vol. 60, no. 4, pp. 248-257. https://doi.org/10.1136/bjsports-2025-110403

Abuley, IK, Meno, LF & Hansen, JG 2026, 'Active Ingredient and Application Timing Determine the Efficacy of Fungicides on Late Blight (Phytophthora infestans)', Potato Research, vol. 69, no. 1, 29. https://doi.org/10.1007/s11540-025-09982-7

Terkelsen, JH, Miscov, R, Jensen, TSR, Schack, A, Grønhøj, MH, Korshøj, AR , Haldrup, M, Poulsen, FR, Fugleholm, K, Bjarkam, CR & Olsen, MH 2026, 'Active subperiosteal vs. passive subdural 24-h drainage following single burr hole evacuation of chronic subdural hematoma: statistical analysis plan for the multicenter, randomized, non-inferiority clinical trial SUPERDURA', Trials, vol. 27, no. 1, 129. https://doi.org/10.1186/s13063-026-09434-8

Pitfield, J , Christiansen, MPV & Hammer, B 2026, 'Active Δ-learning with universal potentials for global structure optimization', Physical Chemistry Chemical Physics, vol. 28, no. 1, pp. 912-926. https://doi.org/10.1039/d5cp04302f

Bromer, FD 2026, 'Activin receptor blockade in the treatment of disuse osteosarcopenia and obesity-related bone deterioration in mice', PhD, Aarhus University.

Alfheim, HB, Svenningsen, H, Holm, A , Ågård, AS, Åkerman, E, Lind, R, Nielsen, AH & Collet, MO 2026, 'Activities and Time Spent on Rehabilitation in Scandinavian ICUs: A Cross-Sectional Survey Among Healthcare Professionals', Acta Anaesthesiologica Scandinavica, vol. 70, no. 4, e70227. https://doi.org/10.1111/aas.70227

Brandi, U 2026, 'Actualising workplace learning opportunities in Danish retail: young adults’ work practices and learning', Journal of Education and Work. https://doi.org/10.1080/13639080.2026.2643591

Ali, K, Shehzad, K, Gul, S, Ajwad, SA & Mehmood, A 2026, 'Actuator Fault-Tolerant Control of Anthropomorphic Manipulator Using Adaptive Backstepping and Neural Estimation of LuGre Friction Torque', Machines, vol. 14, no. 2, 156. https://doi.org/10.3390/machines14020156

Weldingh, FLU , Herlin, MK , Steffensen, EH , Heide-Jørgensen, U , Vogel, I & Christiansen, CF 2026, 'Acute kidney injury and chronic kidney disease in individuals with Down syndrome: a nationwide cohort study', Clinical Kidney Journal, vol. 19, no. 2, sfaf402. https://doi.org/10.1093/ckj/sfaf402

Andersen, OE 2026, 'Acute Metabolic and Functional Effects of β-hydroxybutyrate on Working Skeletal Muscle', PhD, Aarhus University.

Pedersen, CH, Jensen, RD , Rölfing, JD & Gamborg, ML 2026, 'Adaptation and validation of a Danish version of the Physiciansʼ Reactions to Uncertainty Scales', Danish Medical Journal, vol. 73, no. 1, A05250375. https://doi.org/10.61409/A05250375

Eggertsen, CN, Vestergaard, ET, Simonsen, MB, Warner, TC, Frøkjær, JB, Handberg, A, Olesen, LG, Grøntved, A, Olesen, AV, Hagstrøm, S, Brønd, JC & Larsen, RG 2026, 'Adding High-Intensity Interval Training to a Multidisciplinary Lifestyle Intervention for Childhood Obesity: Secondary Analysis of a Randomized Controlled Trial to Examine the Effects on Physical Activity Behavior', Scandinavian Journal of Medicine & Science in Sports, vol. 36, no. 1, e70185. https://doi.org/10.1111/sms.70185

Kasper, P & Feilberg, A 2026, 'Addition of CuEDTA to a full-scale air scrubber eliminates emissions of H2S and methanethiol from livestock facilities', Journal of Environmental Chemical Engineering, vol. 14, no. 2, 121123. https://doi.org/10.1016/j.jece.2026.121123

Kopew, JS, Ritskes-Hoitinga, M, Singer, M, Trunnell, ER, Vidaurre, G & Krebs, CE 2026, 'Addressing animal methods bias through government-led initiatives', Frontiers in Toxicology, vol. 8, 1761759. https://doi.org/10.3389/ftox.2026.1761759

Skovhus Prior, K, Bentsen, KK, Lietzen, LW, Jørgensen, TL, Schnack, TH, Ørum, M, Jeppesen, SS, Michelsen, L, Ryg, J, Winther, SB & Lund, C 2026, 'Addressing geriatric oncology in Danish cancer guidelines to meet future challenges', Danish Medical Journal, vol. 73, no. 1, A06250480. https://doi.org/10.61409/A06250480

Overwijk, RH, Kolbinger, FR, Eijgelsheim, M, Dekkers, OM, Kronbichler, A & Bajema, IM 2026, 'A Decade of C3 Glomerulopathy: A Nationwide Cohort Study', Kidney International Reports, vol. 11, no. 2, 103681. https://doi.org/10.1016/j.ekir.2025.11.007

Blechingberg, J , Skipper, KA , Beck-Nielsen, SS & Gregersen, PA 2026, 'A deep intronic PHEX variant in a large Danish family with hereditary hypophosphatemia and a milder skeletal, but more severe dental phenotype', Bone, vol. 202, 117666. https://doi.org/10.1016/j.bone.2025.117666

Moradi, H, Hashemi Tari, P, Zomorodian, ZS & A. Mirzaei, P 2026, 'A deep learning CGAN-CFD-based model for fast and accurate airflow prediction in indoor spaces', Journal of Building Engineering , vol. 117, 114638. https://doi.org/10.1016/j.jobe.2025.114638

de Castro, AF & Yıldırım, HC 2026, 'A defect-aware fatigue life framework for corroded mooring chains integrating cyclic plasticity and crack growth', International Journal of Fatigue, vol. 204, 109386. https://doi.org/10.1016/j.ijfatigue.2025.109386

the Scientific and Research Committee of the E-AHPBA 2026, 'Adenosquamous Carcinoma of the Pancreas: Outcomes of a Multicenter European Study (ADESQUPAN Project)', Annals of Surgical Oncology. https://doi.org/10.1245/s10434-025-19051-5

Yao, H, Zhou, Y, Li, L, Gillies, MB, Brikell, I, Gao, L, Wimberley, T, Xie, T, Zhang-James, Y, Astrup, A , Dalsgaard, S , Semark, BD, Engeland, A, Faraone, SV, Klungsøyr, K, Larsson, H, Man, KKC, Snieder, H, Wong, ICK, Yuen, ASC, Zoega, H, Hartman, C & Chang, Z 2026, 'ADHD and adherence to antihypertensive medication treatment: a multinational cohort study', BMC Medicine, vol. 24, no. 1, 122. https://doi.org/10.1186/s12916-026-04714-1

CogEx Research Team 2026, 'Adherence and compliance with exercise training in progressive multiple sclerosis: Rates and correlates from the CogEx trial', Multiple Sclerosis and Related Disorders, vol. 105, 106859. https://doi.org/10.1016/j.msard.2025.106859

Rosendal, LB, Bundgaard, C, Zhang, J , Olsen, A, Rostgaard-Hansen, AL, Dahm, CC & Ibsen, DB 2026, 'Adherence to the EAT-Lancet diet and risk of colorectal cancer in the general population and among individuals with diabetes: a cohort study', European Journal of Nutrition, vol. 65, no. 3, 90. https://doi.org/10.1007/s00394-026-03936-6

Notario Barandiaran, L, Bui, LP, Madan, JC, Peacock, JL, Khatchikian, C & Karagas, MR 2026, 'Adherence to the EAT-Lancet Planetary Health Diet during Pregnancy and Associations with Preterm Birth and Infant Size: a prospective analysis from the New Hampshire Birth Cohort', The American Journal of Clinical Nutrition, pp. 101241. https://doi.org/10.1016/j.ajcnut.2026.101241

Norouzi, S, Moldrup, P, Moseley, B, Robinson, D, Or, D, Hohenbrink, TL, Minasny, B, Sadeghi, M, Arthur, E, Tuller, M, Greve, MH & de Jonge, LW 2026, 'A differentiable hybrid modeling approach for learning soil water retention mechanisms from partial knowledge and data', Journal of Hydrology, vol. 668, 135008. https://doi.org/10.1016/j.jhydrol.2026.135008

Bak Herrie, M & Enni, S 2026, 'A Different View: Investigating Image Collections with Juxtapositional Inpainting', Leonardo.

Galsky, MD, Gschwend, JE, Milowsky, MI, Schenker, M, Valderrama, BP, Tomita, Y, Bamias, A, Lebret, T, Shariat, SF, Park, SH, Agerbaek, M, Jha, G, Stenner, F, Ye, D, Giudici, F, Connors, J, Gupta, S, Chhibber, A, Zhang, J, Bajorin, DF & Witjes, JA 2026, 'Adjuvant nivolumab versus placebo for high-risk muscle-invasive urothelial carcinoma: 5-year efficacy and ctDNA results from CheckMate 274^☆', Annals of Oncology, vol. 37, no. 1, pp. 69-78. https://doi.org/10.1016/j.annonc.2025.09.139

Guo, Y, Chen, R, Allaoui, H, Choudhary, A & Li, W 2026, 'A dual attention graph neural network framework for sustainable supply chain optimization under energy performance contracting', Transportation Research Part E: Logistics and Transportation Review, vol. 208, 104702. https://doi.org/10.1016/j.tre.2026.104702

Mortensen, J, Kristensen, L, Munkholm, M, Hanel, B, Abrahamsen, J, Sidenius, K, Grønlund, B, Hilberg, O, Maltbæk, N, Titlestad, IL, Dahl, R , Bibby, BM, Kryger, SN, Weinreich, UM, Schmid, JM , Bendstrup, E , Paludan, JPD , Hyldgaard, C, Danielsen, LM, Dahl, LS, Jørgensen, E, Jensen, TT, Ellingsgaard, TK, Fuglø, D, Hovind, P, Berg, RMG & Madsen, F 2026, 'Adult reference values for spirometry, body plethysmography and diffusing capacity adjusted for sex, age, weight, height or arm span - the Danish lung function material (DALFUMAT) study', European Clinical Respiratory Journal, vol. 13, no. 1, 2606556. https://doi.org/10.1080/20018525.2025.2606556

Grand, J, Lau, FF, Hassager, C, Bro-Jeppesen, J, Møller, JE, Jakobsen, JC, Taccone, FS, Russo, JJ, Ameloot, K, Skrifvars, MB, van Diepen, S & Kjærgaard, J 2026, 'Advanced Hemodynamic Monitoring During Postresuscitation Care After Out-of-Hospital Cardiac Arrest: Protocol for a Scoping Review', Acta Anaesthesiologica Scandinavica, vol. 70, no. 4, e70224. https://doi.org/10.1111/aas.70224

Gregersen, CH, Aggerholm-Pedersen, N, Sandfeld-Paulsen, B & Winther-Larsen, A 2026, 'Advanced Lung Cancer Inflammation Index has Prognostic Impact in Stage I Non-Small Cell Lung Cancer: A Registry-Based Analysis of 934 Patients', Clinical Lung Cancer, vol. 27, no. 1, pp. 24-33. https://doi.org/10.1016/j.cllc.2025.10.013

Abidi, SMH, Hassan, SA, Raza, SM & Beliatis, MJ 2026, 'Advances in Face Recognition: A Comprehensive Review of Feature Extraction and Dataset Evaluation', Electronics, vol. 15, no. 2, 338. https://doi.org/10.3390/electronics15020338

Thomsen, M, Bruhn, A, Kotta, J, Buck, B, Hall, JR, Martin, G, Agüera, A, Maar, M, Chakraborty, M, Ekoule, M, Overton, I & Budhathoki, M 2026, 'Advancing a restorative blue Economy: A DPSIR–Ecosystem services framework for sustainable seaweed aquaculture', Ecosystem Services, vol. 77, 101800. https://doi.org/10.1016/j.ecoser.2025.101800

Sill, M, Schrimpf, D, Patel, A, Sturm, D, Jäger, N, Sievers, P, Schweizer, L, Banan, R, Reuss, D, Suwala, A, Korshunov, A, Stichel, D, Wefers, AK, Hau, AC, Boldt, H, Harter, PN, Abdullaev, Z, Benhamida, J, Teichmann, D, Koch, A, Hench, J, Frank, S, Hasselblatt, M, Mansouri, S, Díaz de Ståhl, T, Serrano, J, Ecker, J, Selt, F, Taylor, M, Ramaswamy, V, Cavalli, F, Berghoff, AS, Bison, B, Blattner-Johnson, M, Buchhalter, I, Buslei, R, Calaminus, G, Dikow, N, Dohmen, H, Euskirchen, P, Fleischhack, G, Gajjar, A, Gerber, NU, Gessi, M, Gielen, GH, Gnekow, A, Gottardo, NG, Haberler, C, Hamelmann, S, Hans, V, Hansford, JR, Hartmann, C, Heppner, FL, Driever, PH, von Hoff, K, Thomale, UW, Tippelt, S, Frühwald, MC, Kramm, CM, Schüller, U, Schittenhelm, J, Schuhmann, MU, Stein, M, Ketteler, P, Ladanyi, M, Jabado, N, Jones, BC, Jones, C, Karajannis, MA, Ketter, R, Kohlhof, P, Kordes, U, Reinhardt, A, Kölsche, C, Lamszus, K, Lichter, P, Maas, SLN, Mawrin, C, Milde, T, Mittelbronn, M, Monoranu, CM, Mueller, W, Mynarek, M, Northcott, PA, Pajtler, KW, Paulus, W, Perry, A, Blümcke, I, Plate, KH, Platten, M, Preusser, M, Pietsch, T, Prinz, M, Reifenberger, G, Kristensen, BW, Kool, M, Hovestadt, V, Ellison, DW, Jacques, TS, Varlet, P, Etminan, N, Acker, T, Weller, M, White, CL, Witt, O, Herold-Mende, C, Debus, J, Krieg, S, Wick, W, Snuderl, M, Aldape, K, Brandner, S, Hawkins, C, Horbinski, C, Thomas, C, Wesseling, P, von Deimling, A, Capper, D, Pfister, SM, Jones, DTW & Sahm, F 2026, 'Advancing CNS tumor diagnostics with expanded DNA methylation-based classification', Cancer Cell, vol. 44, no. 2, pp. 340-354.e2. https://doi.org/10.1016/j.ccell.2025.11.002

Biskjaer, MM , Frich, J & Halskov, K 2026, Advancing Creative Redesign in HCI Education: Introducing the i3 Method. in In Proceedings of the 8th Annual Symposium on HCI Education (EduCH 2026). ACM Press New York, NY, USA.

Turner, A, Kuperberg, M, McGovern, H, Wrobel, A, Dean, OM, Berk, M, Sylvia, L, McElroy, SL, Tohen, M, Shelton, RC, Iosifescu, DV, Thase, ME, Kocsis, JH, Ostacher, MJ, Deckersbach, T, McInnis, MG, Köhler-Forsberg, O & Nierenberg, AA 2026, 'Adverse childhood experiences and cardiometabolic risk factors in people with bipolar disorder', Journal of Affective Disorders, vol. 393, no. Part A, 120270. https://doi.org/10.1016/j.jad.2025.120270

Bjerge, B & Bach, JS 2026, Ældre med rusmiddelbrug. in T Kolind & S Karsberg (eds), Rusmidler i det danske samfund. En grundbog. Akademisk Forlag.

Bresciani, R, Sarti, C, Rizzo, A, Carvalho, PN , Kisielius, V, Rødland, ES, Lasio, F, Martinuzzi, N & Masi, F 2026, 'Aerated wetland for the treatment of combined sewer overflow: Long-term monitoring of Merone full-scale system', Journal of Environmental Management, vol. 401, 128834. https://doi.org/10.1016/j.jenvman.2026.128834

Displaying results 51 to 100 out of 389685

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen