Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

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Mardahl, M, Hansen, EP, Fromm, B, Borup, A , Whitehead, B , Boysen, AT, Stensballe, A, Andersen, KL, Williams, AR, Jex, AR, Gasser, RB, Fredholm, M, Thamsborg, SM & Nejsum, P 2025, 'Analysis of Oesophagostomum dentatum excretory-secretory molecules and extracellular vesicles uncovers a repertoire of microRNAs shared with A. Suum and T. Suis and ES containing Paz and Piwi domain proteins implicated in RNAi signaling', International Journal for Parasitology. https://doi.org/10.1016/j.ijpara.2025.10.006

Hong, Z, Tesic, N & Bofill-De Ros, X 2025, 'Analysis of Processing, Post-Maturation, and By-Products of shRNA in Gene and Cell Therapy Applications', Methods and Protocols, vol. 8, no. 2, 38. https://doi.org/10.3390/mps8020038

Chedevergne, F, Yang, J, Stroh, A & Forooghi, P 2025, 'Analysis of Separation in the Roughness Sublayer Using DNS Data and DANS/DEM Modelling of Roughness Effects', Flow, Turbulence and Combustion, vol. 114, no. 3, 021202, pp. 713-735. https://doi.org/10.1007/s10494-024-00585-9

Sun, Y, Sutton, M & Wei, S 2025, 'Analysis strategy for ultrafast X-ray photon correlation spectroscopy', MRS Communications, vol. 15, no. 3, 100561, pp. 406-413. https://doi.org/10.1557/s43579-025-00716-x

Raj, RK, Kumar, A, Rezaeiyan, Y , Klarskov, P , Moradi, F & Shreya, S 2025, Analytical Approach to Engineer Strain-Gradient for Magnetic Skyrmion-based LeakyIntegrate and Fire Neuronal Dynamics. in IEEE Computer Society Annual Symposium on VLSI, ISVLSI 2025 - Conference Proceedings. IEEE Computer Society Press, Proceedings of IEEE Computer Society Annual Symposium on VLSI, ISVLSI, 28th IEEE Computer Society Annual Symposium on VLSI, ISVLSI 2025, Kalamata, Greece, 06/07/2025. https://doi.org/10.1109/ISVLSI65124.2025.11130237

Müller-Bravo, TE, Galbany, L, Stritzinger, MD, Ashall, C, Baron, E, Burns, CR, Höflich, P, Morrell, N, Phillips, M, Suntzeff, NB & Uddin, SA 2025, 'Analyzing Type Ia supernovae near-infrared light curves with principal component analysis', Astronomy and Astrophysics, vol. 702, A134. https://doi.org/10.1051/0004-6361/202555078

Reed, MS, Jensen, EA, Noles, S, Conneely, D, Kendall, H, Raley, M, Tarrant, A, Oakley, N, Hinson, C, Hoare, V, Marshall, K & Pugliese, L 2025, 'Analyzing who is relevant to engage in environmental decision-making processes by interests, influence and impact: The 3i framework', Journal of Environmental Management, vol. 373, 123437. https://doi.org/10.1016/j.jenvman.2024.123437

Kloster, TK 2025 'An Ambit Field Framework for the Full Panel of Day-ahead Electricity Prices'.

Schaefer, Z, Thomsen, TR, Schulz, H-J, Thayssen, FM, Christensen, M, Lindholdt, L , Svendsen, K , Mathisen, A & Grønbæk, K 2025, An Analysis of COVID-19 Recovery Duration: Smartwatch Activity and Self-Report. in 2025 47th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), Copenhagen, Denmark. vol. 2025, IEEE, Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), pp. 1-7. https://doi.org/10.1109/EMBC58623.2025.11251653

Lim, MH, Chan, HH & Ong, SQ 2025, 'An annotated image dataset of urban insects for the development of computer vision and deep learning models with detection tasks', Data in Brief, vol. 60, 111673. https://doi.org/10.1016/j.dib.2025.111673

Gil Arboleda, S, Kamburjan, E, Talasila, P & Larsen, PG 2025, 'An architecture for coupled digital twins with semantic lifting', Software and Systems Modeling, vol. 24, no. 5, pp. 1379-1404. https://doi.org/10.1007/s10270-024-01221-w

Esmailbeygi, H , Hatamzadeh, E & Moradi, F 2025, 'An Area-Efficient and Low-Power Switched Capacitor Integrator for Discrete-Time Delta Sigma Modulators', pp. 229-232. https://doi.org/10.1109/ESSERC66193.2025.11213960

Aabling, RR , Rusan, M, Møller, AMJ, Munk-Pedersen, N , Holm, C , Elmengaard, B , Pedersen, M & Møller, BK 2025, 'A Narrative Review on Manufacturing Methods Employed in the Production of Mesenchymal Stromal Cells for Knee Osteoarthritis Therapy', Biomedicines, vol. 13, no. 2, 509. https://doi.org/10.3390/biomedicines13020509

Petersen, LB, Bogh, SB, Hansen, PM, Milling, L, Knudsen, JS, Pedersen, H, Christensen, EF , Væggemose, U, Folke, F, Wolthers, SA, Christensen, HC, Brøchner, AC & Mikkelsen, S 2025, 'An assessment of long-term complications following prehospital intraosseous access: A nationwide study', Resuscitation, vol. 206, 110454. https://doi.org/10.1016/j.resuscitation.2024.110454

Liebermann, R 2025, 'Anathea E. Portier-Young, The Prophetic Body. Embodiment and Mediation in Biblical Prophetic Literature. Oxford, Oxford University Press, 2024. 344 p. 15 × 23. $120.00', Biblica, vol. 106, no. 1, pp. 141-144.

Jensen, TSR, Hakon, J, Olsen, MH, Gulisano, HA, Obbekjær, T, Poulsen, FR & Mathiesen, TI 2025, 'A national study of burnout, psychosocial work environment, and moral distress among neurosurgical doctors in Denmark', Acta Neurochirurgica, vol. 167, no. 1, 53. https://doi.org/10.1007/s00701-025-06468-w

Kristensen, K , Gouliaev, A , Rasmussen, TR & Christensen, NL 2025, 'A national study of lung cancer patients below 50 years: Variations in characteristics and outcomes by age', Cancer Epidemiology, vol. 99, 102949. https://doi.org/10.1016/j.canep.2025.102949

Skaarup, KG, Johansen, ND, Brandi, L, Lindhardt, MK, Bech, JN, Svensson, M, Kristensen, T, Thuesen, AD, Knudsen, MG, Kampmann, JD, Hornum, M, Ørts, B, Modin, D, Lassen, MCH, Janstrup, KH, Claggett, BL, Vaduganathan, M, Bhatt, AS, Van Spall, HGC, Jensen, JUS, Zannad, F, Solomon, SD, Møller, A, Borg, R, Birn, H, Hansen, D & Biering-Sørensen, T 2025, 'A Nationwide Factorial Randomized Trial of Electronic Nudges to Patients with Chronic Kidney Disease and Their General Practices for Increasing Guideline-Directed Medical Therapy: The NUDGE-CKD Trial', Circulation, vol. 152, no. 6, pp. 369-383. https://doi.org/10.1161/CIRCULATIONAHA.125.075403

Kallsberg, A, Slotved, HC, Gaini, S & Krogfelt, KA 2025, 'A nationwide study of two decades of invasive pneumococcal disease in the Faroe Islands, 2000–2023', Infectious Diseases, vol. 57, no. 12, pp. 1095-1104. https://doi.org/10.1080/23744235.2024.2440033

Li, H , Zhang, H, Zhao, P & Fang, L 2025, 'An atlas of cell type specific regulatory effects in cattle', Nature Genetics.

Yang, Y, Wang, Y, Liu, T, Wang, M, Sun, M, Song, S, Fan, W & Huang, G 2025, 'Anatomical prior-based vertebral landmark detection for spinal disorder diagnosis', Artificial Intelligence in Medicine, vol. 159, 103011. https://doi.org/10.1016/j.artmed.2024.103011

George, AF, Neidleman, J, Luo, X, Frouard, J, Elphick, N, Yin, K, Young, KC, Ma, T, Andrew, AK, Ezeonwumelu, IJ, Pedersen, JG, Chaillon, A, Porrachia, M, Woodworth, B, Jakobsen, MR, Thomas, R, Smith, DM, Gianella, S & Roan, NR 2025, 'Anatomical, subset, and HIV-dependent expression of viral sensors and restriction factors', Cell Reports, vol. 44, no. 1, 115202. https://doi.org/10.1016/j.celrep.2024.115202

Yang, S, Chung, JW, Park, SH, Zhang, J, Lee, K, Hwang, D, Lee, KS, Na, SH, Doh, JH, Nam, CW, Kim, TH, Shin, ES, Chun, EJ, Choi, SY, Kim, HK, Hong, YJ, Park, HJ, Kim, SY, Husic, M, Lambrechtsen, J, Jensen, JM , Nørgaard, BL, Andreini, D, Maurovich-Horvat, P, Merkely, B, Penicka, M, de Bruyne, B, Ihdayhid, A, Ko, B, Tzimas, G, Leipsic, J, Sanz, J, Rabbat, MG, Katchi, F, Shah, M, Tanaka, N, Nakazato, R, Asano, T, Terashima, M, Takashima, H, Amano, T, Sobue, Y, Matsuo, H, Otake, H, Kubo, T, Takahata, M, Akasaka, T, Kido, T, Mochizuki, T, Yokoi, H, Okonogi, T, Kawasaki, T, Nakao, K, Sakamoto, T, Yonetsu, T, Kakuta, T, Yamauchi, Y, Taylor, CA, Bax, JJ, Shaw, LJ, Stone, PH, Narula, J & Koo, BK 2025, 'Anatomical vs Physiological Lesion Characteristics in Prediction of Acute Coronary Syndrome', JACC: Cardiovascular Interventions, vol. 18, no. 23, pp. 2833-2845. https://doi.org/10.1016/j.jcin.2025.09.006

Nichols, G , Randahl-Beltran, ES , Gentili, M , Sønderskov, M & Melander, B 2025, An attempt to holistically evaluate services and dis-services of fall vegetation in 30 cropping systems. in 20TH EUROPEAN WEED RESEARCH SOCIETY SYMPOSIUM: Joint approaches for sustainable weed management. European Weed Research Society, pp. 43, 20th European Weed Research Society Symposium, Lleida, Spain, 01/07/2025.

Ágústsson, SÝ , Haque, MA , Truong, TT , Bianchi, M, Klyuchnikov, N, Mottin, D , Karras, P & Hofmann, P 2025, 'An autoencoder for compressing angle-resolved photoemission spectroscopy data', Machine Learning: Science and Technology, vol. 6, no. 1, 015019. https://doi.org/10.1088/2632-2153/ada8f2

Arbejdsgruppen om almen praksis 2025, Anbefalinger om de forebyggende sundhedsydelser til børn, unge og deres forældre: Tilrettelæggelse og indhold. Sundhedsstyrelsen, København. <https://www.sst.dk/-/media/Udgivelser/2025/Forebyggende-sundhedsydelser-boern-og-unge/Anbefalinger-om-forebyggende-sundhedsydelser-til-boern-og-unge-og-deres-foraeldre.ashx>

Fjederholt, ET & Sunde, P 2025, 'Anbefalinger til håndtering af ulve i nærheden af menneskelig aktivitet: Med særlig fokus på bymæssig bebyggelse', 26 p., Fagligt notat fra DCE – Nationalt Center for Miljø og Energi (2020-...), no. 54, vol. 2025. <https://dce.au.dk/fileadmin/dce.au.dk/Udgivelser/Notater_2025/N2025_54.pdf>

Jensen, NR 2025, 'Anbragt. Stemmer fra børneforsorgen fra 1900 til i dag: ingen', Uddannelseshistorie: årbog fra Selskabet for dansk Skolehistorie, vol. 58, pp. 183-185.

Neville, M 2025, Andelsboligforeningsloven med kommentarer. in Karnovs Lovsamling . Karnov Group.

Olesen, NW 2025, 'Anders Foghs skuffende selvbiografi efterlader centrale spørgsmål ubesvarede', Altinget.dk. <https://www.altinget.dk/artikel/2-aer-anders-foghs-skuffende-selvbiografi-efterlader-centrale-spoergsmaal-ubesvarede>

Jespersen, A & Hejná, M 2025, 'Andetsprogstaleren får ondt i identiteten, når du skifter over til engelsk', Kognition og Pædagogik, vol. 136, pp. 56-64.

Brøndum, T 2025, '‘And now I feel I don’t have a clear future’: Hauntings and temporal uncertainty in refugee narratives.', Journal of Refugee Studies, vol. 38, no. 3, feaf011, pp. 526-540. https://doi.org/10.1093/jrs/feaf011

Kristensen, HK 2025, Andre danske cistercienserklostre: planer og udformning. in E Rosengren, P Wranning & AH (eds), Ås kloster: Om grundandet, godset och byggnaderna. Hallands länsmuseer, Halmstad, Hallands länsmuseers skriftserie, vol. 14, pp. 33-82.

Kølvraa, CL 2025, Andy Nowicki: Alt-Right novelist and incel storyteller. in B Forchtner & M Feldman (eds), The Bloomsbury Handbook to Far-Right Fiction. Bloomsbury Academic.

de Haro Reyes, B, Palmé, A, Fitzgerald, H, Göransson, M, Lyytikäinen, V, Madsen, B , Normand, S, Thorbjörnsson, H, Treier, UA & Hagenblad, J 2025, 'An east–west distribution of genetic diversity in Nordic populations of caraway (Carum carvi L.) and its consequences for conservation prioritisation', Conservation Genetics, vol. 26, no. 4, e0244666, pp. 771-785. https://doi.org/10.1007/s10592-025-01702-5

Kristensen, SB , Kristensen, TM , Barfoed, S & Handberg, S 2025, 'An Eclectic Collection: Re-Examining the Heroon of Kalydon and its Sculptures', Proceedings of the Danish Institute at Athens, vol. XI, pp. 52-73.

van der Spek, M, Bardow, A, Baum, CM, Bolongaro, V, Dufour-Decieux, V, Esch, C, Fritz , L, Garcia, S, Hamann, C, Hondeborg, D, Kiani, A, Lueck, S, Patel, SK, Peh, SB, Pisciotta, M, Psarras, P, Repke, T, Sáenz-Cavazos, PA, Schulte, I, Shu, D, Shu, Q, Sovacool, B, Strefler, J, Vallejo Castaño, S, Wang, J-Y, Wessling, M, Wilcox, J, Young, J & Minx, JC 2025, 'An ecosystem of carbon dioxide removal reviews: part 1: direct air CO2 capture and storage', Energy & Environmental Science, vol. 18, no. 22, pp. 9713-9785. https://doi.org/10.1039/D5EE01732G

Petersen, AP 2025, 'A need-supportive intervention to increase trial retention', PhD, Aarhus University.

Wu, M 2025, 'An Effective and Practical Self-healing Concept for Cement-based Materials', 5th International Conference on the Chemistry of Construction Materials, Garching, Germany, 08/09/2025 - 10/09/2025.

Liu, L, Domenzain Gonzalez, D, Vela, I , Maurya, PK , Kühl, AK , Bording, T, Ejlskov, P, Auken, E , Larsen, JJ & Christiansen, AV 2025, 'An efficient cross-borehole direct current resistivity monitoring instrument', Geophysics, vol. 90, no. 2, pp. D61-D70. https://doi.org/10.1190/geo2023-0518.1

Schrøder, I , Laage-Thomsen, J & Ratner, HF 2025, An Emerging Welfare Data Economy? in K Asdal & L Dogonova (eds), Value economy: versions, sites, and tools. Mattering Press.

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Enemærke, VJ , Kristensen, A , Rost, LG, Skovsgaard, E & Jørgensen, KA 2025, 'An Enantioselective Nucleophilic Aromatic tele-Substitution', Journal of the American Chemical Society, vol. 147, no. 41, pp. 37854-37863. https://doi.org/10.1021/jacs.5c14328

Cummings, C , Gratz, S, Kirkman, E, Letz, JC, Rock, JD & Špenko, Š 2025 'An equivalence linking CM-types $A_\infty$ and $D_\infty$'.

Soni, S , Rezaeiyan, Y, Boehnert, T, Ferreira, R, Kaushik, BK, Moradi, F & Shreya, S 2025, 'A Neuromorphic Computational Model for Spintronics-based Hopfield Oscillatory Neural Network', IEEE Transactions on Magnetics, vol. 61, no. 9, 7001105. https://doi.org/10.1109/TMAG.2025.3539977

Jadoul, Y, Hersh, TA, Domingos, EF, Gamba, M, Favaro, L & Ravignani, A 2025, 'An evolutionary model of rhythmic accelerando in animal vocal signalling', PLoS Computational Biology, vol. 21, no. 4, e1013011. https://doi.org/10.1371/journal.pcbi.1013011

Hematpur, H, Hosseini, S, Mahmood, SM, Abdollahi, R, Hamdi, Z & Ghamarpoor, R 2025, 'A new approach to foam flooding modelling with novel parameter Estimation techniques', Scientific Reports, vol. 15, no. 1, 22829. https://doi.org/10.1038/s41598-025-05847-8

Fernández, SS & Sadow, L 2025, A new approach to teaching intercultural pragmatics: using minimal languages in the language classroom. in C Ackermann-Boström, B Hellqvist & M Håkansson Ramberg (eds), Alla språk i skolan : perspektiv på språkundervisning. Uppsala university, Uppsala, Studia Didactica Upsaliensia, vol. 16, pp. 57-70. <https://uu.diva-portal.org/smash/get/diva2:1950620/FULLTEXT01.pdf>

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Displaying results 5501 to 5550 out of 391118

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Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen