Exosomes

Exosomes are small membrane vesicles of endocytic origin secreted by most cell types in vitro. Cells continuously secrete a large number of microvesicles, exosomes and small molecules into the extracellular space. Exosomes have been shown to contain mRNA and miRNA and they are able to mediate communication between cells, by transport of RNA and proteins which can be functionalized in the recipient cell. The ability to transport bioactive cargoes, gives exosomes innate therapeutic potential as a natural drug carrier. Efficient techniques for loading drugs into exosomes to utilize the natural carrier capabilities of these vesicles remains to be elucidated.

Applications of Exosomes

The presence exosomes in blood, saliva, and urine paves the way for the possible application of exosomes as a diagnostic marker for diseases. Many diseases are difficult to detect by the methods and equipment available today and there is a growing demand for development of new non‐invasive methods to detect diseases in early stages. The use of exosomes as markers of disease is being investigated to cement their potential as a non-invasive diagnostic tool for early detection of many diseases, in particular cancers. This, however, relies on thorough characterisation of exosomes in both healthy and diseased states

An important factor to understanding the roles of exosomes is knowledge of their biodistribution. Currently, the number of publications describing the biodistribution of exosomes is limited. The main reason being the lack of a robust fluorescent label that can be visualised in vivo. Mapping the specific cell type derived exosomes can increase our knowledge on the roles of exosomes in the organism, as well as help to identify natural targeting systems.

Isolation, Characterisation and Fluorescent Labelling

The most common isolation protocol uses a differential centrifugation to fractionate cells, microvesicles, and exosomes, which are pelleted by ultracentrifugation. After vesicles are secreted from cells into the extracellular environment, it difficult to separate the different subpopulations of vesicles to obtain a pure sample. We have developed methods to optimise isolation and thorough characterisation procedures to obtain pure and well-characterised exosomes. Furthermore, we have developed a simple method for labelling of exosomes with a robust fluorescent label that can be visualised in vivo, allowing investigation of the biodistribution of different cell derived exosomes.

Collaborators:

Department of Clinical Medicine, Aarhus University Hospital, Denmark

Dan Peer, Department of Cell Research & Immunology, Tel Aviv University, Israel

Moein Moghimi, school of Medicine, Pharmacy and Health, Durham University, UK

Recent Publications

Sort by: Date | Author | Title

Rudnick, G & Sinning, S 2019, 'F1000Prime Recommendation of [Coleman JA et al., Nature 2019 569(7754):141-145].', F1000Prime Reports. https://doi.org/10.3410/f.735602477.793560490

Liu, X, Toprakcioglu, Z, Dear, AJ, Levin, A, Ruggeri, FS, Taylor, CG, Hu, M, Kumita, JR, Andreasen, M, Dobson, CM, Shimanovich, U & Knowles, TPJ 2019, 'Fabrication and Characterization of Reconstituted Silk Microgels for the Storage and Release of Small Molecules', Macromolecular Rapid Communications, vol. 40, no. 8, 1800898. https://doi.org/10.1002/marc.201800898

Zhao, H , Yang, Q , Wang, Z, Zhao, H, Liu, B, Chen, Q & Dong, M 2019, 'Fabrication of 2D Hetero-Complexes With Nucleic-Acid-Base Adenine and Fatty-Acid Stearic Acid at Liquid/Solid Interface', Frontiers in Chemistry, vol. 7, 513. https://doi.org/10.3389/fchem.2019.00513, https://doi.org/10.3389/fchem.2019.00513

Haahr, L 2019, Facebook Contradictions in Municipal Social Media Practices. in A Molnar (ed.), Strategic Management and Innovative Applications of E-Government. IGI global, pp. 51-71. https://doi.org/10.4018/978-1-5225-6204-7.ch003

Cone, L 2019, 'Facebook er en del af byen, akkurat som den lokale bager', Politiken. <https://politiken.dk/debat/debatindlaeg/art7518361/Facebook-er-en-del-af-byen-akkurat-som-den-lokale-bager>

Ravn, I 2019, 'Facebooks kommende bank vil skabe nye finanskriser og kreditbobler', Politiken, pp. 5.

Ondov, B, Jardine, N, Elmqvist, N & Franconeri, S 2019, 'Face to face: Evaluating visual comparison', IEEE Transactions on Visualization and Computer Graphics, vol. 25, no. 1, 8440856, pp. 861-871. https://doi.org/10.1109/TVCG.2018.2864884

Sant, GN, Pilon, LG, La Plante, E, Wang, B, Wei, Z & Castano, SV Feb. 21 2019, Facile, low-energy routes for the production of hydrated calcium and magnesium salts from alkaline industrial wastes, Patent No. WO2019/0366676 (A1). <https://dk.espacenet.com/publicationDetails/biblio?CC=WO&NR=2019036676A1&KC=A1&FT=D&ND=3&date=20190221&DB=EPODOC&locale=dk_DK#>

Christensen, MK & Pedersen, IM 2019, Facilitated Multi-Source Feedback: exploring a new model for professional development of academic staff. in Exploring new fields through the scholarship of teaching and learning: Third EuroSoTL Conference June 13-14, 2019. Universidad del Pais Vasco * Servicio Editorial, Bilbao, pp. 469-477, EuroSoTL19 , Bilbao , Spain, 13/06/2019. <https://www.ehu.eus/en/web/eurosotl-2019/proceedings>

Elkjær, E & OToole, MS 2019, 'Facilitating approach behavior with emotion regulation: A study of healthy individuals', International Convention of Psychological Science (ICPS), Paris, France, 07/03/2019 - 09/03/2019. <https://www.psychologicalscience.org/conventions/icps2019/icps-2019-program>

Arntzen, C, Moe, S, Aadal, L & Pallesen, H 2019, 'Facilitating learning and change in the daily lives of stroke survivors: A comparative analysis of municipal stroke rehabilitation services in Norway and Denmark', Cogent Medicine - PUBLIC HEALTH & PRIMARY CARE, vol. 6, 1608080. https://doi.org/10.1080/2331205X.2019.1608080

Stage, C 2019, Faciliteret deltagelse: en typologi til design, udvikling og analyse af deltagelsesprocesser. in B Eriksson, M Houlberg & A Scott Sørensen (eds), Kunst, kultur og deltagelse. Aarhus Universitetsforlag, Aarhus, pp. 21-35.

Pedersen, AY 2019, 'Factfulness og almendannelse i praksis', Sprogforum, vol. 25, no. 69, pp. 48-56. <https://samfundslitteratur.dk/bog/sprogforum-69-dannelse>

Rosati, B , Teiwes, R , Kristensen, K , Bossi, R , Skov, H , Glasius, M , Pedersen, HB & Bilde, M 2019, 'Factor analysis of chemical ionization experiments: Numerical simulations and an experimental case study of the ozonolysis of α-pinene using a PTR-ToF-MS: Numerical simulations and an experimental case study of the ozonolysis of α-pinene using a PTR-ToF-MS', Atmospheric Environment, vol. 199, pp. 15-31. https://doi.org/10.1016/j.atmosenv.2018.11.012

Niu, J, Carlström, J, Berggren, P, Dietz, R , Sveegaard, S & Teilmann, J 2019, 'Factors affecting bycatch rate of harbour porpoise (Phocoena phocoena) in Swedish waters', World Marine Mammal Conference, Barcelona, Spain, 07/12/2019 - 12/12/2019.

Brewer, GE, Clarkson, JJ, Maurin, O, Zuntini, AR, Barber, V, Bellot, S, Biggs, N, Cowan, RS, Davies, NMJ, Dodsworth, S, Edwards, SL, Eiserhardt, WL, Epitawalage, N, Frisby, S, Grall, A, Kersey, PJ, Pokorny, L, Leitch, IJ, Forest, F & Baker, WJ 2019, 'Factors Affecting Targeted Sequencing of 353 Nuclear Genes From Herbarium Specimens Spanning the Diversity of Angiosperms', Frontiers in Plant Science, vol. 10, 1102, pp. 1-14. https://doi.org/10.3389/fpls.2019.01102

Gebremariam, MK, Arah, OA, Bergh, IH, Andersen, LF, Bjelland, M, Grydeland, M & Lien, N 2019, 'Factors affecting the dose of intervention received and the participant satisfaction in a school-based obesity prevention intervention', Preventive Medicine Reports, vol. 15, 100906. https://doi.org/10.1016/j.pmedr.2019.100906

Chaiklieng, S, Suggaravetsiri, P, Kaminski, N & Autrup, H 2019, 'Factors affecting urinary tt-muconic acid detection among benzene exposed workers at gasoline stations', International Journal of Environmental Research and Public Health, vol. 16, no. 21, 4209. https://doi.org/10.3390/ijerph16214209

Boda, R , Mathiasen, JB & Tambo, T 2019, 'Factors and Motives in Practitioners’ Decision Making in Industrial Sourcing: Local Versus Global Perspectives', Paper presented at 26th EurOMA Conference 2019, Helsinki, Finland, 17/06/2019 - 19/06/2019 pp. 1-12.

Yang, TK, Chang, CC, Chang, HC, Yang, HJ & Huang, KH 2019, 'Factors Associated with Bothersome Lower Urinary Tract Symptoms in Middle-Aged Men Receiving Health Checkup', Scientific Reports, vol. 9, 901. https://doi.org/10.1038/s41598-018-37605-4

Meister, P, Liu, B, Khalili, A, Böttcher, ME & Jørgensen, BB 2019, 'Factors controlling the carbon isotope composition of dissolved inorganic carbon and methane in marine porewater: an evaluation by reaction-transport modelling', Journal of Marine Systems, vol. 200, 103227. https://doi.org/10.1016/j.jmarsys.2019.103227

Eilenberg, J, Saussure, S, Ben Fekih, I, Jensen, AB & Klingen, I 2019, 'Factors driving susceptibility and resistance in aphids that share specialist fungal pathogens', Current Opinion in Insect Science, vol. 33, pp. 91-98. https://doi.org/10.1016/j.cois.2019.05.002

Nuntapaitoon, M, Muns, R, Theil, PK & Tummaruk, P 2019, 'Factors influencing colostrum consumption by piglets and their relationship with survival and growth in tropical climates', Livestock Science, vol. 224, pp. 31-39. https://doi.org/10.1016/j.livsci.2019.04.008

Michalski, M, Pągowska-Klimek, I, Thiel, S, Świerzko, AS, Hansen, AG , Jensenius, JC & Cedzyński, M 2019, 'Factors involved in initiation and regulation of complement lectin pathway influence postoperative outcome after pediatric cardiac surgery involving cardiopulmonary bypass', Scientific Reports, vol. 9, 2930. https://doi.org/10.1038/s41598-019-39742-w

Keizer, E, Christensen, MB , Carlsen, AH, Smits, M, Wensing, M, Senn, O & Huibers, L 2019, 'Factors related to out-of-hours help-seeking for acute health problems: a survey study using case scenarios', BMC Public Health, vol. 19, 33. https://doi.org/10.1186/s12889-018-6332-6

Christoffersen, P , Lunde, A & Olesen, KV 2019, 'Factor structure in commodity futures return and volatility', Journal of Financial and Quantitative Analysis, vol. 54, no. 3, pp. 1083-1115. https://doi.org/10.1017/S0022109018000765

Pyšek, P, Guo, WY, Štajerová, K, Moora, M, Bueno, CG, Dawson, W, Essl, F, Gerz, M, Kreft, H, Pergl, J, van Kleunen, M, Weigelt, P, Winter, M & Zobel, M 2019, 'Facultative mycorrhizal associations promote plant naturalization worldwide', Ecosphere, vol. 10, no. 11, e02937. https://doi.org/10.1002/ecs2.2937

Grosen, AK , Povlsen, J , Lemming, LE , Dahl Jørgensen, SM , Dahlerup, JF & Hvas, CL 2019, 'Faecal Microbiota Transplantation Eradicated Extended-Spectrum Beta-Lactamase-Producing Klebsiella pneumoniae from a Renal Transplant Recipient with Recurrent Urinary Tract Infections', Case Reports in Nephrology and Dialysis, vol. 9, no. 2, pp. 102-107. https://doi.org/10.1159/000502336

Ravn, KS (ed.) & Grundtvig, NFS 2019, Fædrene-Aaret. Dannekvad til Hans Majestæt Kong Frederik den Siette. Grundtvigs Værker. <http://www.xn--grundtvigsvrker-7lb.dk/tekstvisning/18459/0#>

Finderup, J & Lomborg, K 2019, Fælles beslutningstagen og beslutningsstøtte i sygeplejen. in B Nielsen & H Barbesgaard (eds), Klinisk beslutningstagen: sygeplejefaglige perspektiver. FADL's Forlag, København, pp. 69-92.

Lodberg, P 2019, 'Fælles om det væsentlige', Aalborg Stiftsbog, vol. 62, pp. 28-33. <https://aalborgstift.dk/_Resources/Persistent/a/d/7/2/ad7232ca10c3e82e61773b59905597919a6b891d/Aalborg%20Stiftsbog%202019_WEB.pdf>

Hansen, HR 2019, Fælles om fællesskabende didaktikker - men hvordan. EMU - Danmarks Læringsportal.

Broström, S 2019, 'Fællesskab og solidaritet: Vejen til livet - om Makarenkos kollektivopdragelse og dansk gruppepædagogik', Dansk Pædagogisk Tidsskrift, vol. 2019, no. 3, pp. 68-75.

Damsgaard, JB 2019, Fænomenologi og hermeneutik som grundlag for forståelse i sygeplejen: Hvori består dannelsen, perspektiverne og forståelsen? in BH Hansen (ed.), Fænomenologi og hermeneutik: Anvendelse og argumentation i sygeplejen. Samfundslitteratur, Kbh., pp. 17-30.

Damsgaard, JB 2019, Fænomenologi og hermeneutik som grundlag for handling i sygeplejen: Hvordan åbner man sig for den menneskelige eksistens? in B Heimann Hansen (ed.), Fænomenologi og hermeneutik: Anvendelse og argumentation i sygeplejen. Samfundslitteratur, Kbh., pp. 189-204.

Bryld Staunæs, A & Benjamin Asger Krog Møller 2019, 'Fagkritisk Rapport', Monsieur Antipyrine, vol. 4/5, pp. 345-372. <https://www.academia.edu/77989032/Fagkritisk_Rapport>

Markvorsen, S, Christensen, TS, Petersen, CK, Højte, S, Lyndrup, O, Olesen, MN & Rønning, F 2019, Faglighed i gymnasiet: Matematik - Delrapport 2. Gymnasiepædagogik, vol. Særnummer 2019, vol. 2, Syddansk Universitet, Odense. <https://www.uvm.dk/publikationer/2020/200109-delrapport-2-matematik>

Togeby, O, Hobel, P, Qvortrup, A, Haaning, K & Ljungdalh, AK 2019, 'Faglighed i gymnasiet. Dansk. Delrapport 4', Gymnasiepædagogik.

Grøn, CH & Møller, AM 2019, 'Faglig ledelse: Hvad er det? Og hvordan ser det ud i praksis?', Ledelse i Morgen - Tidsskrift for pædagogisk ledelse, vol. 23, no. 1.

Lund, L , Boie, MAK & Andersen, M (eds) 2019, Faglig Pædagogisk Ledelse – Didaktisk refleksion som grundlag for MUS og GRUS. Center for Undervisningsudvikling og Digitale Medier, Aarhus Universitet, Aarhus. <https://tdm.au.dk/fileadmin/tdm/NYT_INDHOLD_2019/Forskning/Projekter/FPL/SAMLET_FPL-LederErfaringsbog.pdf>

Kai, P , Sørensen, JN , Melander, B , Ottosen, C-O , Børsting, CF , Bertelsen, MG , Petersen, KK & Jensen, PK 2019, 'Faglig redegørelse vedrørende teknologiliste 2019 til brug i forbindelse med ordningen om tilskud til investeringer i nye miljøteknologier', No. 2019-760-001067, 55 p., Oct 09, 2019..

Sørensen, P 2019, 'Fagligt grundlag til fastsættelse af nye udnyttelsesprocenter til gødningstyperne under anden organisk gødning end husdyrgødning', No. 2018-760-001240, 3 p., Apr 10, 2019..

Bengtsen, SS , Nordentoft, HM, Jensen, TW, Troelsen, R, Ankersborg, V, Pogner, K-H & Larsen, O 2019, 'Faglig vejledning under forandring: Tendenser i institutionel og vejledningsdidaktisk praksis', DUN Konferencen 2019, Odense, Denmark, 28/05/2019 - 29/05/2019.

Holm, TE 2019, 'Faglig vurdering af et skydearrangements effekt på ynglende vandrefalk', 4 p., Notat fra DCE - Nationalt Center for Miljø og Energi (2011-2019).

Rölfing, JHD, Nørskov, JK, Paltved, C, Konge, L & Andersen, SAW 2019, 'Failure affects subjective estimates of cognitive load through a negative carry-over effect in virtual reality simulation of hip fracture surgery', Advances in Simulation, vol. 4, 26. https://doi.org/10.1186/s41077-019-0114-9

Skovsgård, SPH 2019, 'Failure of composite materials by kink band formation'. https://doi.org/10.7146/aulsps-e.337

Bennedsen, J & Caspersen, ME 2019, 'Failure rates in introductory programming: 12 years later', ACM Inroads, vol. 10, no. 2, pp. 30-36. https://doi.org/10.1145/3324888

Nørskov, S , Damholdt, MF , Ulhøi, JP , Jensen, MB , Mathiasen, MK, Ess, CM & Seibt, J 2019, 'Fairness perceptions in job interviews: Using a teleoperated robot as a fair proxy', Paper presented at 35th European Group for Organizational Studies (EGOS) Colloquium, Edinburgh, United Kingdom, 04/07/2019 - 06/07/2019.

Adrian, L, Druckman, D, Filzmoser, M, Damholdt, MF, Koeszegi, ST, Navarro Guerrero, N , Seibt, J , Smedegaard, CV , Vestergaard, C & Quick, O 2019, 'Fair Proxy Mediation - First Experimental Results with Tele‐Operated Robots', Paper presented at International Conference on Group Decision and Negotiation in 2019 a Joint GDN-EWG/BOR meeting, Loughborough, United Kingdom, 11/06/2019 - 15/06/2019.

Kraglund, RA & Jacobsen, LB 2019, Fake News. in G Agger, P Jauert & K Schrøder (eds), Medie- og Kommunikationsleksikon. Samfundslitteratur, Frederiksberg. <https://medieogkommunikationsleksikon.dk/fake-news/>

Displaying results 97451 to 97500 out of 389685

1950

Revised 08.12.2025

Lise Refstrup Linnebjerg Pedersen